THE STUDY OF VDR FOKI RS2228570 SNP IN AUTOIMMUNE THYROIDITIS.

Autoimmune thyroid disease (AITD) is a common organ-specific autoimmune disease. A strong influence of genetic and epigenetic modifications has been demonstrated to take part in the development and progression of autoimmune thyroid diseases. The linkage between the Vitamin D receptor (VDR) polymorphism and several autoimmune disorders, including the AITD. In this article, we aim to investigate the Frequency of VDR Fokl (rs2228570) genotypes (CC, CT, TT) and alleles (C,T) in autoimmune thyroiditis. The investigation of VDR Fokl (rs2228570) was conducted on 150 samples (control (75 healthy women) and diseased women (75 diseased with autoimmune thyroiditis)) patients from the Adjara (Georgia) Population. It also examined some clinical and laboratory characteristics of the study population. Autoimmune thyroiditis's disease was diagnosed by measuring blood antibodies, determining the level of thyroperoxidase, and conducting an ultrasound examination. Anti-TPO and TSH were studied using the ELISA method. The genomic DNA was extracted from the peripheral blood. The polymerase chain reaction was evaluated to examine the VDR Fokl rs2228570 SNP polymorphism. According to VDR Fokl (rs2228570) genotypes (CC; CT, TT) frequency, in the control group, the Frequency of CC-genotype is 48%, CT-heterozygous genotype is 29.33%, and TT-genotype is 22.67%; in the diseased population, the Frequency of CC-genotype is 57.33%, CT-genotype is 34.67%, and TT-genotype is 8%. According to VDR Fokl (rs2228570) alleles (C, T), the Frequency of the C-allele is high, and the Frequency of the T-allele is low in both populations. The Frequency of the CC and CT genotypes of VDR Fokl (rs2228570) is high in the population with autoimmune thyroiditis compared to the control group; the TT genotype is relatively low in the population suffering from autoimmune thyroiditis; According to VDR Fokl (rs2228570) alleles (C, T), the Frequency of C-allele is high both population.

[Vaginosis in patients with antenatal foetus death].

Polymerase chain reaction (PCR) analysis of bacterial vaginosis as well as of vaginal anaerobe flora (Mycoplasma Hominis, Ureaplasma urealyticum, Gardnerella vaginalis, Bacteroides spp. and Mobiluncus curtisii) was performed in women with antenatal foetus death. Specimens from forty women with this pathology were studied. Control group consisted of 100 pregnant women of the adequate age groups and gestation periods, but with live foetus. Vaginal smears treated by the use of polymerase chain reaction method showed that in the cases of antenatal foetus death in 70% of women Lactobacillus spp. was completely absent. This bacterium was observed only in 30% of these women. In 75% of women with live foetus Lactobacillus spp. was observed, while in 25% it was not present. Increase of quantity of anaerobic vaginal flora was observed in women, where the antenatal death of foetus was diagnosed. Percentage significance of these indices in women with live foetus was comparatively lower.