Mechanical Properties of Fresh, Frozen and Vitrified Articular Cartilage.

Osteochondral allograft transplantations are typically used to treat focal articular cartilage injuries where the damaged cartilage is replaced with fresh cadaveric donor grafts. Despite the notable success rate of this procedure, it is limited by fresh donor tissue availability which can only be stored for approximately 28 days after harvest. Vitrification, a form of cryopreservation, can extend the storage time of cartilage. Although it has shown to preserve chondrocyte viability, its effect on the mechanical properties of the tissue has not been thoroughly investigated. Therefore, in this study, the mechanical properties of fresh, frozen, and vitrified articular cartilage were evaluated through unconfined compression testing. Results showed that the peak modulus, equilibrium modulus, and relaxation time constants of the vitrified and control samples (tested one day after harvest) were similar and higher than the fresh (tested 21 days after harvest) and frozen samples. This demonstrated that vitrification does not adversely affect the mechanical properties of cartilage and can be used as an alternative to fresh allografts which are limited by storage time. The fresh samples also had inferior mechanical properties compared to the control samples suggesting that vitrified allografts could potentially improve clinical outcomes in addition to increasing donor tissue availability.

Vitrified Particulated Articular Cartilage for Joint Resurfacing: A Swine Model.

BACKGROUND: The use of particulated articular cartilage for repairing cartilage defects has been well established, but its use is currently limited by the availability and short shelf life of donor cartilage. Vitrification is an ice-free cryopreservation technology at ultralow temperatures for tissue banking. An optimized vitrification protocol has been developed for particulated articular cartilage; however, the equivalency of the long-term clinical efficacy of vitrified particulated articular cartilage compared with fresh articular cartilage has not yet been determined. HYPOTHESIS: The repair effect of vitrified particulated cartilage from pigs would be equivalent to or better than that of fresh particulated cartilage stored at 4°C for 21 days. STUDY DESIGN: Controlled laboratory study. METHODS: A total of 19 pigs were randomly divided into 3 experimental groups: fresh particulated cartilage group (n = 8), vitrified particulated cartilage group (n = 8), and negative control group (no particulated cartilage in the defect; n = 3). An additional pig was used as the initial cartilage donor for the first set of surgical procedures. Pigs were euthanized after 6 months to obtain femoral condyles, and the contralateral condyle was used as the positive (no defect) control. Samples were evaluated for gross morphology using the Outerbridge and Osteoarthritis Research Society International (OARSI) scoring systems, histology (safranin O, collagen type I/II, DAPI), and chondrocyte viability using live-dead membrane integrity staining. RESULTS: There were no infections after surgery, and all 19 pigs were followed for the duration of the study. The OARSI grades for the fresh and vitrified particulated cartilage groups were 2.44 ± 1.35 and 2.00 ± 0.80, respectively, while the negative control group was graded significantly higher at 4.83 ± 0.29. Analysis of histological and fluorescent staining demonstrated that the fresh and vitrified particulated cartilage groups had equivalent regeneration within cartilage defects, with similar cell viability and densities and expression of proteoglycans and collagen type I/II. CONCLUSION: The implantation of fresh or vitrified particulated cartilage resulted in the equivalent repair of focal cartilage defects when evaluated at 6 months after surgery. CLINICAL RELEVANCE: The vitrification of particulated cartilage is a viable option for long-term storage for cartilage tissue banking and could greatly increase the availability of donor tissue for transplantation.

Polycarbonate-urethane coating can significantly improve talus implant contact characteristics.

Talus implants can be utilized in cases of talus avascular necrosis and has been regarded as a promising treatment method. However, existing implants are made of stiff materials that directly oppose natural cartilage. The risk of long-term cartilage wear and bone fracture from the interaction between the cartilage and stiff implant surfaces has been documented in post-hemiarthroplasty of the hip, knee and ankle joints. The aim is to explore the effects of adding a layer of compliant material (polycarbonate-urethane; PCU) over a stiff material (cobalt chromium) in talus implants. To do so, we obtained initial ankle geometry from four cadaveric subjects in neutral standing to create the finite element models. We simulated seven models for each subject: three different types of talus implants, each coated with and without PCU, and a biological model. In total, we constructed 28 finite element models. By comparing the contact characteristics of the implant models with their respective biological model counterparts, our results showed that PCU coated implants have comparable contact area and contact pressure to the biological models, whereas stiff material implants without the PCU coating all have relatively higher contact pressure and smaller contact areas. These results confirmed that adding a layer of compliant material coating reduces the contact pressure and increases the contact area which in turn reduces the risk of cartilage wear and bone fracture. The results also suggest that there can be clinical benefits of adding a layer of compliant material coating on existing stiff material implants, and can provide valuable information towards the design of more biofidelic implants in the future.

Quantitative analysis of regional specific pelvic symmetry.

Understanding bilateral pelvic symmetry can be useful for analyzing complex pelvis anatomy and simplifying difficult procedures for pelvic fractures. This paper aims to quantify the degree of regional pelvic symmetry using computer-based methods. CT scans of 30 intact pelvises were digitized into 3D models and regions were defined: the ilium, acetabulum, pubis, and ischium. The right hemipelvis was aligned with the left, and deviations between the two models were quantified using method 1 (global registration) and method 2 (local registration). Symmetry was evaluated using the root mean square (RMS) of the deviations and the percentage of points within preset thresholds of ± 2 mm and ± 1 mm. The results showed that > 86% of points are within the ± 2 mm deviation threshold and average RMS are < 1.33 mm. For all regions, method 2 showed lower deviations than method 1. The pubis and ischium regions showed a large difference in symmetry between the two methods indicating high local symmetry, but a degree of global asymmetry. Conversely, the acetabular and iliac regions showed similar levels of symmetry with the two methods. When evaluated locally, the pelvic regions can be considered highly symmetric; the acetabulum is highly symmetric globally as well. These findings can be used in future studies to assess the feasibility of patient-specific implants using the mirrored contralateral hemipelvis as a template for unilateral pelvic fracture fixation. The left image shows the "cut planes" used to define four pelvic regions: the ilium, acetabulum, pubis, and ischium. The right image shows a deviation color map (DCM) used to quantify bilateral pelvic symmetry. The scale and color illustrate the degree of deviation of the left hemipelvis with the right hemipelvis with the units in millimeters (mm).

Development and application of the average pelvic shape in virtual pelvic fracture reconstruction.

BACKGROUND: With unilateral pelvic fractures, the contralateral hemipelvis can be used as a template in virtual reconstruction; however, this cannot be applied for bilateral fractures. Therefore, statistical shape modelling was used to build average pelvic shapes that can serve as templates when reconstructing bilaterally fractured pelvises. METHODS: Four average shape models were created for male and female, left and right hemipelves from 20 male and 20 female subjects. They were used as templates to reconstruct eight unilaterally fractured pelvises. RESULTS: The average root-mean-square of deviations between the reconstructed and intact hemipelves was 1.46 ± 0.32 mm, which is less than the 2 mm threshold for causing hip joint complications. CONCLUSION: This indicates that the reconstructions are reliable and the average shape models can be used to reconstruct bilaterally fractured pelvises. The proposed technique can potentially provide quick and accurate treatment plans for pelvic fracture patients, which is necessary for recovery.

Virtual reconstruction of unilateral pelvic fractures by using pelvic symmetry.

PURPOSE: Pelvic fractures are known to be one of the most difficult injuries to treat. The objective of this study is to introduce a novel technique for virtual unilateral pelvic fracture reconstruction. Since the pelvis exhibits remarkable left-right symmetry, the contralateral hemipelvis can be used as a template for rebuilding the fractured hemipelvis. METHODS: CT scan data of the pelvic region of eight subjects with acute unilateral pelvic fractures were involved in this study. Computer-aided design software was used to create 3D models of these pelvises. The contralateral hemipelvis of each subject was then reflected across the sagittal plane, and the fractured hemipelvis was rebuilt by aligning the bone fragments with their equivalent location on the reflected side. To evaluate the quality of this reduction process, a 3D deviation analysis was conducted to calculate the differences between the reflected intact hemipelvis and the reconstructed hemipelvis. RESULTS: Results showed that the average root mean square (RMS) of deviations and average percentage of points within a ± 2 mm predefined threshold was 1.32 ± 0.22 mm and 88.4 ± 3.78%, respectively. The deviation color maps obtained indicated that the largest differences were along the fracture lines and on the non-articular surfaces of the pelvises. CONCLUSION: These results allowed us to conclude the validity of this procedure, since the average RMS difference was below 2 mm and the average percentage of points within ± 2 mm was high. The proposed technique will allow surgeons to provide their patients with more accurate reconstruction procedures which can potentially improve surgical outcomes.

Investigation of pelvic symmetry using CAD software.

Severe pelvic fractures often prove difficult for surgeons as they require patient-specific surgical treatment plans and customized equipment. Developing virtual patient-specific 3D pelvis models would ease the surgical planning process and enable development of custom fixation plates. This paper aims to examine pelvic symmetry to conclude whether the contralateral side may be used as a reference model for the fractured side of the pelvis. Fourteen subjects with intact pelvises were involved in this study. CT scans of the pelvises were converted to 3D models and the right sides of the pelvises were reflected and aligned with the left sides. A deviation analysis was then performed for each set of models and results showed that the average root mean square (RMS) of values was 1.14 ± 0.26 mm and the average percentage of points below a deviation threshold of ± 2 mm was 91.9 ± 5.55%. The deviation color maps (DCMs) showed that the largest deviations were on the non-articular surfaces. The volume and surface area of each model were also examined and showed no significant differences between left and right sides. These results indicate that the pelvis displays bilateral symmetry and this concept can be used to develop fully intact patient-specific 3D pelvis models for fracture reconstruction using the unfractured contralateral side. Graphical Abstract.