RESUMEN
Polymerase chain reaction (PCR) for the identification of C.botulinum of type A was developed. As primers, oligonucleotides corresponding to sequences 913 -- 932 and 1852 -- 1871 of the gene of type A botulinic neurotoxin were used. The study revealed that under optimum conditions the positive result of the reaction was registered only when the DNA of C.botulinum strains of type A (11 strains) was used, but not that of C.botulinum strains of other types (11 strains of type B, 5 strains of type C, 2 strains of type D, 6 strains of type E and 1 strain of type G). High sensitivity, specificity and rapidity of PCR open good prospects for its practical use.
Asunto(s)
Clostridium botulinum/genética , Reacción en Cadena de la Polimerasa/métodos , Secuencia de Bases , Toxinas Botulínicas/genética , Cartilla de ADN , ADN Bacteriano/análisis , ADN Bacteriano/genética , Electroforesis en Gel de Agar , Datos de Secuencia Molecular , Sensibilidad y EspecificidadRESUMEN
In the pig heart sarcolemma, a 65 kDa protein is found to be ADP-ribosylated by Clostridium botulinum ADP-ribosyltransferase (exoenzyme C3). ADP-ribosylation of this protein is regulated by guanyl nucleotides and cytosol factor in a fashion similar to that for other C3 substrates. The new exoenzyme C3 substrate was partially purified. This protein is supposed to be a GTP-binding one.