Pedigree analysis of pre-breeding efforts in Trifolium spp. germplasm in New Zealand.

BACKGROUND: Prebreeding in plants is the activity designed to identify useful characteristics from wild germplasm and its integration in breeding programs. Prebreeding aims to introduce new variation into the populations of a species of interest. Pedigree analysis is a valuable tool for evaluation of variation in genebanks where pedigree maps are used to visualize and describe population structure and variation within these populations. Margot Forde Germplasm Centre (MFGC) is New Zealand's national forage genebank and holds a collection of ~ 75 species of the genus Trifolium, of which only a dozen have been taken through prebreeding programs. The main objective of this study was to construct pedigree maps and analyse patterns of relatedness for seven minor Trifolium species accessions contained at the MFGC. These species are Trifolium ambiguum, Trifolium arvense, Trifolium dubium, Trifolium hybridum, Trifolium medium, Trifolium subterraneum and the Trifolium repens x Trifolium occidentale interspecific hybrids. We present a history of Trifolium spp. prebreeding in New Zealand and inform breeders of possible alternative forage species to use. RESULTS: Pedigree data from accessions introduced between 1950 and 2016 were used and filtered based on breeding activity. Kinship levels among Trifolium spp. remained below 8% and no inbreeding was found. Influential ancestors that contributed largely to populations structure were identified. The Australian cultivar 'Monaro' had a strong influence over the whole population of accessions in T. ambiguum. T. subterraneum and T. repens x T. occidentale had the largest number of generations (3). T. ambiguum and T. medium had the highest cumulative kinship across the decades. CONCLUSIONS: We conclude that there are high levels of diversity in the seven Trifolium spp. studied. However, collection and prebreeding efforts must be strengthened to maximize utilization and bring useful genetic variation.

Changes in serum haptoglobin as an acute response to a marathon road race.

In order to assess the possible occurrence of acute haemolysis with prolonged exertion, serum haptoglobin levels were determined from venous blood samples collected from eight male runners immediately preceding (PreRH), immediately following (PRH1), and 6 h following (PRH2) completion of a marathon road race. The subjects' mean age, percentage of body fat, and maximum oxygen uptake (VO2max) were 46 +/- 9 years, 12.1 +/- 3.4% and 54.9 +/- 8.4 ml kg-1 min-1, respectively. The mean race finish time for the subjects was 3:35 +/- 0:18 h:min. The PreRH, PRH1 and PRH2 averaged 129 +/-18, 97 +/- 48 and 86 +/- 35 mg dl-1 respectively. Significant differences of -32.5 mg dl-1 between PreRH versus PRH1 and -42.5 mg dl-1 between PreRH versus PRH2 were found. The difference between PRH1 and PRH2 of -10.6 mg dl-1 was not significant. No significant correlations were found between the decreases in serum haptoglobin and VO2max or race finish time. The data suggests to occurrence of an acute haemolysis with performance of the marathon road race.