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Clin Chim Acta ; 445: 122-6, 2015 May 20.
Artículo en Inglés | MEDLINE | ID: mdl-25824633

RESUMEN

Currently molecular techniques are a broadly accepted tool for diagnosis and are able to benefit patients in clinical practice. The polymerase chain reaction (PCR) has been especially incorporated into practical applications that are already in widespread use across the globe. With regard to the initial DNA extraction from clinically relevant samples, a number of commercially available kits are commonly used and are also designed to be easy to handle and less labor-intensive. In this study, the pressure system extracting DNA in column-based kit was developed, and its utility was compared with the centrifuge method using sputum from patients who were diagnosed with pneumonia. Also, due to the compact size and rapid processing time, the practical application of the pressure-based system incorporated into an automated pipetting machine was evaluated through clinical study. Our data suggests that DNA extraction by pressure was capable of serving as a substitute for the centrifuge method, and the compact and automatic nature of the pressure system device provided rapid and valuable information for clinical practice.


Asunto(s)
ADN Bacteriano/aislamiento & purificación , Neumonía Bacteriana/diagnóstico , Extracción en Fase Sólida/métodos , Esputo/química , Centrifugación , Haemophilus influenzae/química , Haemophilus influenzae/crecimiento & desarrollo , Haemophilus influenzae/patogenicidad , Humanos , Klebsiella pneumoniae/química , Klebsiella pneumoniae/crecimiento & desarrollo , Klebsiella pneumoniae/patogenicidad , Moraxella catarrhalis/química , Moraxella catarrhalis/crecimiento & desarrollo , Moraxella catarrhalis/patogenicidad , Neumonía Bacteriana/microbiología , Presión , Pseudomonas aeruginosa/química , Pseudomonas aeruginosa/crecimiento & desarrollo , Pseudomonas aeruginosa/patogenicidad , Juego de Reactivos para Diagnóstico , Reacción en Cadena en Tiempo Real de la Polimerasa , Extracción en Fase Sólida/instrumentación , Streptococcus pneumoniae/química , Streptococcus pneumoniae/crecimiento & desarrollo , Streptococcus pneumoniae/patogenicidad
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