Levels and Trophic Transfer of Selected Pesticides in the Lake Ziway Ecosystem.

The levels of 30 selected pesticides and trophic biomagnification of DDT were investigated in biota samples of the Lake Ziway in the Rift valley region, Ethiopia. Carbon source and trophic position were calculated by using 13C and 15N stable isotopes, individually, and trophic magnification factors (TMFs) were inferred. Only DDT and its metabolites were quantified in all samples analyzed. The most prominent metabolite was p,p'-DDE with mean concentration ranging from the 0.82-33.69 ng g-1 lipid weight. Moreover, the ratio of DDT/DDD + DDE in all the biota samples was less than 1 signifying historical DDT application. Regression of log [ΣDDT] vs TL (trophic level) among all biota species showed a significant correlation, indicating that DDTs are biomagnifying along with the food web of Lake Ziway with an estimated TMF of 2.75. The concentrations of DDTs and other organochlorine pesticides found in biota from Lake Ziway were, in general, lower than studies found in previous studies carried out in the same lake.

Small-scale Farmer Pesticide Knowledge and Practice and Impacts on the Environment and Human Health in Ethiopia.

BACKGROUND: Inappropriate use and application of pesticides in Ethiopia pose a major threat to the health of farmers and the environment. OBJECTIVE: The present study aimed to assess the level of knowledge, attitudes, and practices of small-scale vegetable farmers towards the use of pesticides in Ethiopia along the Lake Ziway watershed. METHODS: This was a cross-sectional study involving a total of 210 farmers randomly selected during a period of pesticide application from a purposively selected village located in the immediate vicinity of Lake Ziway, Ethiopia. Data were generated through structured in-depth interviews and on-site observations on farms. A Chi-square test was applied to evaluate whether the collected data and their probable associations were significant. RESULTS: World Health Organization (WHO) class II pesticides (moderately toxic) were the most frequently used pesticides in the study area. There was no reported use of WHO classes 1a and 1b and banned or restricted pesticides such as dichlorodiphenyltrichloroethane (DDT) and endosulfan. Most (92%) farmers reported indiscriminately disposing of empty containers in the field, while 86.7% applied the leftover pesticides to other crops. More than 90% of small-scale farmers did not use any personal protective equipment (PPE) when handling pesticides. About 95% of farmers had poor knowledge regarding pesticides. A significant association (p < 0.001) was observed between the knowledge of farmers and their practices related to pesticides. CONCLUSIONS: Generally, the knowledge of small-scale farmers on pesticides was poor. Moreover, the inappropriate disposal of pesticides and pesticide containers poses a risk to the environment. The findings of the present study underline the need to train farmers concerning the safe and proper use of pesticides to mitigate hazards to human health and the environment. PARTICIPANT CONSENT: Obtained. ETHICS APPROVAL: The study was granted an exemption from requiring ethics approval from the Hawassa University College of Natural and Computational Science, Research and Review Committee. COMPETING INTERESTS: The authors declare no competing financial interests.

The effect of freezing and thawing on water flow and MCPA leaching in partially frozen soil.

Limited knowledge and experimental data exist on pesticide leaching through partially frozen soil. The objective of this study was to better understand the complex processes of freezing and thawing and the effects these processes have on water flow and pesticide transport through soil. To achieve this we conducted a soil column irrigation experiment to quantify the transport of a non-reactive tracer and the herbicide MCPA in partially frozen soil. In total 40 intact topsoil and subsoil columns from two agricultural fields with contrasting soil types (silt and loam) in South-East Norway were used in this experiment. MCPA and bromide were applied on top of all columns. Half the columns were then frozen at -3 °C while the other half of the columns were stored at +4 °C. Columns were then subjected to repeated irrigation events at a rate of 5 mm artificial rainwater for 5 h at each event. Each irrigation was followed by 14-day periods of freezing or refrigeration. Percolate was collected and analysed for MCPA and bromide. The results show that nearly 100% more MCPA leached from frozen than unfrozen topsoil columns of Hov silt and Kroer loam soils. Leaching patterns of bromide and MCPA were very similar in frozen columns with high concentrations and clear peaks early in the irrigation process, and with lower concentrations leaching at later stages. Hardly any MCPA leached from unfrozen topsoil columns (0.4-0.5% of applied amount) and concentrations were very low. Bromide showed a different flow pattern indicating a more uniform advective-dispersive transport process in the unfrozen columns with higher concentrations leaching but without clear concentration peaks. This study documents that pesticides can be preferentially transported through soil macropores at relatively high concentrations in partially frozen soil. These findings indicate, that monitoring programs should include sampling during snow melt or early spring in areas were soil frost is common as this period could imply exposure peaks in groundwater or surface water.

An integrated approach for assessing influence of agricultural activities on pesticides in a shallow aquifer in south-eastern Norway.

The study examines the influence of agricultural activities on pesticides in groundwater in an area with fluvial deposits of sand with a top layer of sandy silt and silt, intensive cultivation of potatoes and cereals, and drinking water supplies of households from local groundwater wells. Information about local agricultural practice and washing sites for pesticide spraying equipment, properties of soils and deeper deposits, hydrogeology and groundwater flow, simulations of pesticide leaching, and contents of pesticides and nitrate in groundwater samples from drinking water wells was used to explore extension and reasons of pesticide contamination of groundwater. Pesticides were found in a majority of the sampled wells. Eight different pesticides and metabolites were detected in groundwater samples. The results demonstrate that on fluvial deposits diffuse pollution from spraying of fields with pesticides can result in groundwater contamination in Nordic climate. Higher concentrations of pesticides in some wells can be explained by point source contamination from washing sites. The occurrence of pesticides in drinking water wells touches up the question whether pesticides should be given general approvals, or approvals should include restrictions or recommendations regarding use on areas with high risk of groundwater contamination. Combination of washing sites for pesticide spraying equipment and groundwater wells for drinking water requires attention, proper equipment and practice, and knowledge about pesticides, soil and water to avoid contamination. Samples from wells adjacent to washing sites for pesticide equipment might overestimate average pesticide concentrations in groundwater bodies. In Nordic areas attention should be given to pesticide pollution of shallow groundwater in fluvial deposits. To provide basis for interpretation of results and planning of mitigation measures against pesticide contamination, an integrated approach using information about agronomical practice and point sources, soil properties, hydrogeology and simulations of pesticide leaching is recommended for future surveys and monitoring of pesticides in groundwater.

Organochlorine pesticides and polychlorinated biphenyls in fish from Lake Awassa in the Ethiopian Rift Valley: human health risks.

Dietary intake of fish containing organic contaminants poses a potential threat to human health. In the present work, an assessment has been carried out to look at the human health risk associated with consumption of fish contaminated with organochlorine pesticides (OCPs) and polychlorinated biphenyles (PCBs) in certain fish species collected from Lake Hawassa, Ethiopia. The health risk assessment was made by comparing the concentrations of OCPs and PCBs in fish muscle tissues with reference doses given in the USEPA guidelines. Dichlorodiphenyltrichloroethanes (DDTs), endosulfans, PCBs and chloridanes were identified in fish species collected from Lake Hawassa. The most predominant pesticides were DDTs, with mean concentrations of ΣDDT ranging from 19 to 56 ng g(-1) wet weights. The highest concentrations of DDTs were found in Barbus intermedius, representing the highest trophic level. PCBs, DDT and endosulfan concentrations found in B. intermedius exceeded the reference dose for children between the ages of 0-1 year (with hazard index of above 1.0). Therefore, consumption of fish from a high trophic level (e.g. B. intermedius) from Lake Hawassa may pose a special health risk to children.

Effects of picoxystrobin and 4-n-nonylphenol on soil microbial community structure and respiration activity.

There is widespread use of chemical amendments to meet the demands for increased productivity in agriculture. Potentially toxic compounds, single or in mixtures, are added to the soil medium on a regular basis, while the ecotoxicological risk assessment procedures mainly follow a chemical by chemical approach. Picoxystrobin is a fungicide that has caused concern due to studies showing potentially detrimental effects to soil fauna (earthworms), while negative effects on soil microbial activities (nitrification, respiration) are shown to be transient. Potential mixture situations with nonylphenol, a chemical frequently occurring as a contaminant in sewage sludge used for land application, infer a need to explore whether these chemicals in mixture could alter the potential effects of picoxystrobin on the soil microflora. The main objective of this study was to assess the effects of picoxystrobin and nonylphenol, as single chemicals and mixtures, on soil microbial community structure and respiration activity in an agricultural sandy loam. Effects of the chemicals were assessed through measurements of soil microbial respiration activity and soil bacterial and fungal community structure fingerprints, together with a degradation study of the chemicals, through a 70 d incubation period. Picoxystrobin caused a decrease in the respiration activity, while 4-n-nonylphenol caused an increase in respiration activity concurring with a rapid degradation of the substance. Community structure fingerprints were also affected, but these results could not be directly interpreted in terms of positive or negative effects, and were indicated to be transient. Treatment with the chemicals in mixture caused less evident changes and indicated antagonistic effects between the chemicals in soil. In conclusion, the results imply that the application of the fungicide picoxystrobin and nonylphenol from sewage sludge application to agricultural soil in environmentally relevant concentrations, as single chemicals or in mixture, will not cause irreversible effects on soil microbial respiration and community structure.

Biomagnification of DDT and its metabolites in four fish species of a tropical lake.

The concentrations and biomagnifications of dichlorodiphenyltrichloroethane (DDT) and its metabolites were examined in four fish species (Clarias gariepinus, Oreochromis niloticus, Tilapia zillii, and Carassius auratus) from Lake Ziway, Rift Valley, Ethiopia. Paired stomach content analysis, and stable isotope ratio of nitrogen (δ(15)N, ‰) and carbon (δ(13)C, ‰) were used to study the trophic position of the fish species in the lake. 4,4'-DDE, 4,4'-DDT and 4,4'-DDD were the main DDTs identified in the fish samples, with 4,4'-DDE as the most predominant metabolite, with mean concentration ranging from 1.4 to 17.8 ng g(-1) wet weight (ww). The concentrations of DDTs found in fish from Lake Ziway were, in general lower than those found in most studies carried out in other African Lakes. However, the presence of DDT in all tissue samples collected from all fish species in the lake indicates the magnitude of the incidence. Moreover, the observed mean 4,4'-DDE to 4,4'-DDT ratio below 1 in C. auratus from Lake Ziway may suggest a recent exposure of these species to DDT, indicating that a contamination source is still present. 4,4'-DDE was found to biomagnify in the fish species of the lake, and increases with trophic level, however, the biomagnification rate was generally lower than what has been reported from other areas. Significantly higher concentrations of 4,4'-DDE were found in the top consumer fish in Lake Ziway, C. gariepinus than in O. niloticus (t=2.6, P<0.01), T. zillii (t=2.5, P<0.02) and C. auratus (t=2.2, P<0.03).

Bioaccumulation of persistent organic pollutants (POPs) in fish species from Lake Koka, Ethiopia: The influence of lipid content and trophic position.

The concentrations and bioaccumulation of persistent organic pollutants (POPs) were determined in four fish species from Lake Koka, Ethiopia, representing 2-3 levels in the food chain of the lake. Dichlorodiphenyltrichloroethanes (DDTs), endosulfans, polychlorinated biphenyls (PCBs) and chlorpyrifos were identified, with DDTs as the most predominant pesticide, with concentration ranging from 0.05 to 72.53ngg(-1) wet weight (ww). All fish tissue samples collected from different species of the lake contained residues of DDTs. The maximum level of DDTs was found in the fattiest, African sharptooth catfish (Clarias gariepinus) sampled from the lake, with a mean concentration of 15.15ngg(-1)ww. The significant (P<0.05) relationship between concentrations of DDTs and δ(15)N indicates that DDTs biomagnified in the food web of the lake. The 4,4'-DDE to 4,4'-DDT ratio in Oreochromis niloticus (0.6) and Cyprinus carpio (0.5) were below 1, indicating ongoing use of DDTs in the study area and recent exposure of these fish species.

Metribuzin transport in undisturbed soil cores under controlled water potential conditions: experiments and modelling to evaluate the risk of leaching in a sandy loam soil profile.

BACKGROUND: Mobility of pesticides in soils is often evaluated and characterised in the surface soil layers rather than at different depths where soil characteristics such as soil organic matter, microbial biomass or clay contents can strongly change pesticide behaviour. The objective of this work was to characterise the reactivity of the herbicide metribuzin in three main soil horizons found in the 0-80 cm profile of an alluvial soil of southern Norway under dynamic transport conditions. RESULTS: A laboratory infiltrometer was used to perform percolation experiments in soil cores sampled in the three horizons Ap, Bw and Bw/C, at a fixed matric potential of -10 cm, thus preventing pores of equivalent radii higher than 0.015 cm from contributing to water flow. The physical equilibrium transport model correctly described the transport of water tracer (bromide). The distribution coefficient K(d) values were estimated to be 0.29, 0.17 ± 0.02 and 0.15 ± 0.00 L kg(-1) for horizons Ap, Bw and Bw/C respectively, in close agreement with batch sorption data. Degradation was found only for the surface horizon with a short half-life of about 5 days, in disagreement with longer half-lives found in batch and field degradation data. CONCLUSION: For all horizons, a kinetic sorption model was needed for better description of metribuzin leaching. Chemical non-equilibrium was greatest in the Bw horizon and lowest in the Bw/C horizon. Overall, metribuzin exhibited a greater mobility in the deeper horizons. The risk of metribuzin transfer to groundwater in such alluvial soils should therefore be considered.

Bioaccumulation of organochlorine pollutants in the fish community in Lake Arungen, Norway.

Organochlorine pollutants in the major fish species (pike Esox lucius, perch Perca fluviatilis, and roach Rutilus rutilus) of Lake Arungen, Norway, were investigated after an extensive removal of large pike in 2004. The organochlorine pollutants detected in fish liver samples in 2005 were dichlorodiphenyltrichloroethane (DDTs), polychlorinated biphenyls (PCBs), hexachlorobenzene (HCB), and heptachlor epoxide (HCE). DDTs were the dominant among all analyzed OCs. Sigma PCB and HCB, detected in fish from two clearly distinct trophic levels (prey and predators), give an indication of biomagnification. All OC concentrations in female pike were significantly lower compared to males, which might be due to the removal of high concentrations of pollutants in roe during spawning.

Effect of freezing and thawing on microbial activity and glyphosate degradation in two Norwegian soils.

Little research has been done on pesticide dissipation in cold climates and there is a need to focus on the influence of climate on pesticide degradation in soil. Glyphosate, N-(phosphonomethyl)glycine, is a herbicide frequently used for controlling perennial weeds through application after harvest and was used as a model compound for this study. The effect of freeze-thaw activity on the availability of glyphosate in soil, and consequently its mineralization by soil microorganisms, was studied through laboratory incubations of repacked soil cores treated with 14C-labelled glyphosate and subjected to different freeze-thaw treatments. Winter simulation regimes applied were constant thaw (+5 degrees C), constant freezing (-5 degrees C), unstable conditions with short fluctuations (24 h of -5 degrees C followed by 24 h of +5 degrees C), and long duration fluctuations (3 weeks of -5 degrees C followed by 3 weeks of +5 degrees C). Distribution of 14C-glyphosate was followed during the incubation through measurements of the mineralized fraction (14CO2), soil water fraction, KOH extractable fraction, and non-extractable fraction. Microbial parameters used to characterize the soils were estimates of size of microbial biomass, overall microbial activity and microbial diversity. The constant freezing treatment exhibited the lowest amount of glyphosate mineralization. The constant thawed treatment and the treatments with fluctuating temperature exhibited significantly increased mineralization. These results were in accordance with the observed concentration of glyphosate in soil water; the higher the activity, the lower the concentration. The amount of glyphosate extractable with KOH and the resulting non-extractable fraction, however, were not significantly affected by soil type or temperature regime. The glyphosate mineralization pattern was comparable with the overall microbial activity in the soils. Observed different levels of diversity might explain some of the difference in total glyphosate mineralization between soils.