Unveiling the phytochemical profile, antioxidant and antibacterial activities, acute toxicity insight and analgesic effect of Retama dasycarpa stems: An unexplored endemic plant from Morocco.

Retama dasycarpa is an endemic shrubby leguminous plant of Morocco used in traditional folk medicine. The plant has never been studied for either its phytochemical or pharmacological properties. This study represents the first investigation of the phytochemical profile as well as the antioxidant, the antibacterial, the analgesic effects and the oral acute toxicity of Retama dasycarpa. Watery and hydromethanolic stems plant macerates have been investigated. Secondary metabolites quantitative analysis was achieved through spectrophotometric techniques. Antioxidant effect was explored through DPPH, ABTS and FRAP trials. Antibacterial activity was investigated using a micro-plates dilution assay. Analgesic activity was explored through acetic acid-induced writhing and tail-flick methods. Acute oral toxicity was investigated on mice. Phytochemical analysis was achieved through UHPLC connected to diode array and mass spectrometry detectors. The obtained results showed significant contents in total phenolics, flavonoids and tannins in both extracts especially the hydromethanolic extract whose contents were slighlty higher than the aqueous one resulting in a remarkable antioxidant activity. Compared to the aqueous extract, the H2O:MeOH (1:1) one showed notable antibacterial activity against the tested strains. The acute toxicity in mice revealed the non-toxicity of the extracts along with a promised starting material of central and peripheral analgesics. The UHPLC analysis revealed the presence of several bioactive phytochemicals pertaining to phenolic acids, flavonols, flavones and isoflavones. The obtained results demonstrate the richness of this endemic and unexplored plant in terms of bioactive compounds and their associated activities, making it a promising source of pharmacological ingredients.

Ultrasonic-assisted extraction for phenolic compounds and antioxidant activity of Moroccan Retama sphaerocarpa L. leaves: Simultaneous optimization by response surface methodology and characterization by HPLC/ESI-MS analysis.

This study was designed to optimize the ultrasound-assisted extraction of phenolic compounds and the antioxidant activity of Moroccan Retama sphaerocarpa extracts using response surface methodology (RSM). A central composite design has been conducted to investigate the effects of three factors: extraction period (X1), solvent concentration (X2), and solvent-to-material ratio (X3) on extraction yield, total phenolic content (TPC), flavonoids content (TFC), and antioxidant activity. The obtained results showed that the experimental values agreed with the predicted ones, confirming the capacity of the used model for optimizing the extraction conditions. The best extraction conditions for the simultaneous optimization were an extraction time of 38 min, a solvent concentration of 58%, and a solvent-to-material ratio of 30 mL/g. Under these conditions, the optimized values of yield, TPC, TFC, and DPPH-radical scavenging activity (DPPHIC50) were 18.91%, 154.09 mg GAE/g, 23.76 mg QE/g, and 122.47 µg/mL, respectively. The further HPLC/ESI-MS analysis of the obtained optimized extract revealed the presence of 14 phenolic compounds with piscidic acid, vitexin, and quinic acid as major compounds. These research findings indicate promising applications for efficiently extracting polyphenolic antioxidants, especially in the food industry.