Free medial plantar flap connection with a posterior tibial artery flap and its modification for combined dorsal and plantar foot defects - two case reports.

Reconstruction of large soft tissue foot defects were considered a difficult issue due to weight-bearing function of the foot. The reconstruction becomes more difficult when both plantar and dorsal soft tissues are involved. The options for the reconstruction were variable, in 2016 Hao Wu et al. presented a combined flap for coverage of combined fore-foot plantar and dorsal soft tissue defects. We used combined flaps in two cases and present our experience in this article.

Controlling wind turbine tower vibration under external force by applying control systems combination.

The global focus has recently shifted away from fuel-based power sources, and one of the most important projects for energy production is wind energy. To maintain low costs, the current research examines the problem of vibrations affecting wind turbine towers' performance (WTTs). In particular, the tower, resulting from excessive vibrations, can negatively affect a structure's power output and service life, as it can cause fatigue. Therefore, we conducted numerical tests on various types of controlled systems. Our tests revealed that combining a new technique cubic negative velocity control (CNVC) and linear negative acceleration control (LNAC) was the most effective and cost-efficient option for vibration damping. This solution was derived by using an approximation method for the averaging technique. The external force is an important component of a nonlinear dynamic system and can be characterized by two-degree-of-freedom (2-DOF) differential coupled equations. After implementing the control measures, we conducted a numerical analysis of the vibration values before and after the operation. Stability is studied numerically. The numerical and approximate solutions were confirmed through the frequency response equation and time history with fourth-order Runge-Kutta (RK-4). Finally, we investigated the effect of parameters and compared our results with those of previously published studies.

Molecular detection, risk factors, and phylogenetic analysis of tick-borne pathogens in dogs from northern Vietnam.

In tropical regions, numerous tick-borne pathogens (TBPs) play a crucial role as causative agents of infectious diseases in humans and animals. Recently, the population of companion and pet dogs has significantly increased in Vietnam; however, information on the occurrence of TBPs is still limited. The objectives of this investigation were to determine the occurrence rate, risk factors, and phylogenetic characteristics of TBPs in dogs from northern Vietnam. Of 341 blood samples tested by PCR, the total infection of TBPs was 73.9% (252/341). Babesia vogeli (18SrRNA gene - 30.5%) was detected most frequently in studied dogs followed by Rickettsia spp. (OmpA gene - 27%), Anaplasma platys (groEL gene - 22%), Bartonella spp. (16SrRNA - 18.8%), Mycoplasma haemocanis (16SrRNA - 9.4%) and Hepatozoon canis (18SrRNA gene - 1.2%), respectively. All samples were negative for Ehrlichia canis and Anaplasma phagocytophylum. Co-infection was detected in 31.4% of the samples (107/341) of which, A. platys/Bartonella spp. (34/94,10%), Rickettsia spp./B. vogeli (19/94, 5.6%), and M. haemocanis/B. vogeli (19/94, 5.6%) were recorded as the three most frequent two species of co-infection types. Statistical analysis revealed a significant correlation between TBP infection and several host variables regarding age, breed, and living area in the current study. The recent findings reported herein, for the first time in Vietnam, are essential for local veterinarians when considering the appropriate approaches for diagnosing these diseases. Furthermore, this data can be used to establish control measures for future surveillance and prevention strategies against canine TBPs in Vietnam.

The effect of butyric acid and nucleotides supplementation on broiler (Gallus gallus domesticus) growth performance, immune status, intestinal histology, and serum parameters.

Background: Butyric acid and its derivatives support the immune system, lessen inflammation, and lessen oxidative stress in broilers in addition to preserving gut homeostasis and epithelial integrity. Broiler performance has also been demonstrated to rise with the addition of nucleotides to the diet. Aim: The purpose of the study was to ascertain the effects of butyric acid and nucleotides added to feed on the overall performance, immunity, oxidant/antioxidant enzyme levels, intestinal histology, and hepatic functions of broilers. Methods: Four experimental groups of thirty chickens, each were used in the present study. The groups were assigned as a control group that received normal diet without additives, butyrate (B) group received the diet supplemented with butyric acid (250 g/ton feed), nucleotides (N) group received the diet supplemented with nucleotides (200 g/ton feed), and the fourth group received the diet supplemented with a combination of butyrate and nucleotide (BN) (250 g/ton B feed, and 200 g/ton N feed, respectively). Necrotic enteritis was produced in ten birds from each group to assess the immune-modulatory effect of these supplements, antioxidant status, intestinal histology, and liver functions were measured in all experimental groups. Results: The addition of butyric acid and nucleotides to feed enhanced body weight, growth performance, hepatic functions, and antioxidant capabilities. Histological sections of the gut from challenged or unchallenged (with necrotic enteritis) groups in the BN group showed considerable improvement, as shown by strong proliferation in intestinal crypts and villus enterocytes. Conclusion: Nucleotides and butyric acid can be added to broiler feeding regimens to enhance growth and health.

Different controllers for suppressing oscillations of a hybrid oscillator via non-perturbative analysis.

To arrive at an equivalent linear differential equation, the non-perturbative approach (NPA) is established. The corresponding linear equation is employed for performing the structural analysis. A numerical computation demonstrates a high consistency with the precise frequency. The correlation with the numerical solution explains the reasonableness of the obtained solutions. For additional nonlinear kinds of oscillation, the methodology gives an exact simulation. The stable construction of the prototype is shown in a series of diagrams. Positive position feedback (PPF), integral resonant control (IRC), nonlinear integral positive position feedback (NIPPF), and negative derivative feedback (NDF) are proposed to get rid of the damaging vibration in the system. It is found that the NDF control is more efficient than other controllers for vibration suppression. The theoretical methodology is applied by using the averaging method for getting a perturbed solution. The stability and influence of various parameters of the structure are established at main and 1:1 internal resonance, which is presented as one of the worst resonance cases. Association concerning mathematical solution and computational simulation is achieved.

Molecular detection, risk factors, and phylogenetic analysis of tick-borne pathogens in dogs from northern Vietnam

@#In tropical regions, numerous tick-borne pathogens (TBPs) play a crucial role as causative agents of infectious diseases in humans and animals. Recently, the population of companion and pet dogs has significantly increased in Vietnam; however, information on the occurrence of TBPs is still limited. The objectives of this investigation were to determine the occurrence rate, risk factors, and phylogenetic characteristics of TBPs in dogs from northern Vietnam. Of 341 blood samples tested by PCR, the total infection of TBPs was 73.9% (252/341). Babesia vogeli (18SrRNA gene – 30.5%) was detected most frequently in studied dogs followed by Rickettsia spp. (OmpA gene – 27%), Anaplasma platys (groEL gene – 22%), Bartonella spp. (16SrRNA – 18.8%), Mycoplasma haemocanis (16SrRNA – 9.4%) and Hepatozoon canis (18SrRNA gene – 1.2%), respectively. All samples were negative for Ehrlichia canis and Anaplasma phagocytophylum. Co-infection was detected in 31.4% of the samples (107/341) of which, A. platys/Bartonella spp. (34/94,10%), Rickettsia spp./B. vogeli (19/94, 5.6%), and M. haemocanis/B. vogeli (19/94, 5.6%) were recorded as the three most frequent two species of co-infection types. Statistical analysis revealed a significant correlation between TBP infection and several host variables regarding age, breed, and living area in the current study. The recent findings reported herein, for the first time in Vietnam, are essential for local veterinarians when considering the appropriate approaches for diagnosing these diseases. Furthermore, this data can be used to establish control measures for future surveillance and prevention strategies against canine TBPs in Vietnam.

Hydrocotyle umbellata L.; a natural source of bioactives to mitigate diabetes mellitus and its complications.

Metabolomics study of Hydrocotyle umbellata L. revealed the richness of its aerial parts in phenolics primarily; quercetin and its glycoside derivatives, which are well-reported to exert antidiabetic activity owing to their powerful antioxidant capacity. Hence, the antioxidant and antidiabetic potentials of the quercetin standardized ethanolic extract of H. umbellata aerial parts were investigated. The antioxidant activity was examined by using in-vitro 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay, while the antidiabetic activity was examined by using in-vitro α-glucosidase inhibitory assay and further confirmed by in-vivo experiments using streptozotocin-induced diabetes in rat model. Interestingly, the standardized ethanolic extract showed significant in-vitro antioxidant activity, and effectively inhibited Saccharomyces cerevisiae α-glucosidase enzyme activity. Moreover, it significantly reduced fasting blood glucose, triglycerides, and cholesterol levels. Thus, H. umbellata is a potential natural candidate to attenuate diabetes mellitus and its altered lipid profile complications, which could be attributed to its quercetin and quercetin glycosides content.

Circulating microRNAs as novel biomarkers for measuring the potency of ginger extract against cyclophosphamide toxicity in rat renal tissues: molecular and histopathological study.

OBJECTIVE: This study aims to explore underlying molecular variations in the expression of miRNAs in kidney tissues of ginger-treated and non-treated cyclophosphamide (CP)-intoxicated rats. MATERIALS AND METHODS:   A total of 40 adult male Wistar rats were randomly divided into four groups of 10 each: Group I (control: received normal food and water), Group II (received ginger at a dose of 300 mg/kg), Group III (received CP 75 mg/kg, i.p.), and Group IV (received the same dose of CP and ginger extract).  Rats received a single injection of 75 mg/kg CP on days 3, 4, 5, 19, 20, and 21. In CP-intoxicated rats, the treatment with ginger extract at a dose of 300 mg/kg was received by oral gavage starting seven days before CP and continuing throughout the duration of the experiment for four weeks. Molecular variations in the expression of miRNAs, apoptotic genes, histological kidney damage, and abnormal kidney function in control, ginger, and CP-intoxicated rats were identified by using real-time RT-PCR Analysis, immunohistochemical, and colorimetric assays. In addition, HPLC analysis and liquid chromatography spectrophotometry analysis using Diphenyl-1-picrylhydrazyl (DPPH) radical, and Β-Carotene-linoleic acid reagents were applied respectively for in-vitro screening of phytoconstituents and antioxidant activity for ginger extract. RESULTS: The kidney tissues of CP-intoxicated rats displayed an increase in lipid peroxidation marker malonaldehyde (MDA), DNA damage, and fibrosis markers like hyaluronic acid (HA) and hydroxyproline Hypx) with a decrease in the superoxide dismutase (SOD) and total antioxidant capacity (TAC). In addition, molecular expressions of mRNA fibrotic genes such as collagen, type 1, alpha 1 (COL1A1), and α-smooth muscle actin (αSMA). Molecular expressions of levels of B-cell lymphoma 2 (BCl-2) mRNA gene were down-regulated, and the expression of mRNA apoptotic; BCL2 associated X gene (Bax), caspase-3, Bax/BCl-2 ratio genes were significantly up-regulated respectively. Moreover, cellular oxidative genes, erythroid 2-related factor (Nrf2), and heme oxygenase-1 (HO-1) were down-regulated, respectively. The miR-155-5p, miR-34a-5p, miR-21-5p significantly increased while the miR-193b-3p, miR-455-3p, and miR-342-3p significantly decreased. Ginger also increased the expression of Nrf2, HO-1, and BCl-2 genes in the kidneys of rats induced with CP. In addition, active phytoconstituents, particularly 6]]-shogaol and 6]]-gingerol, were significantly identified in ginger extract using HPLC analysis. Antioxidant activity of these active metabolites were shown to be higher against in vitro free radicals (DPPH and Β-Carotene-linoleic acid), suggesting the potential antioxidant and antiapoptotic properties of ginger against CP-toxicity. CONCLUSIONS: Treatment with ginger in rats induced with CP resulted in significant improvement in the expression of certain molecular miRNAs. The kidney tissues of these rats showed a marked decrease in the expression of miR-155-5p, miR-34a-5p, and miR-21-5p, while the levels of miR-193b-3p, miR-455-3p, and miR-342-3p were observed to increase significantly. In conclusion, ginger can protect rats from CP-induced nephrotoxicity.

Microplastics in marine invertebrates from the Red Sea Coast of Egypt: Abundance, composition, and risks.

This study marked the first exploration of microplastics in marine invertebrates in the Red Sea Coast of Egypt. 110 individuals from 11 different species, including Bivalvia, Gastropoda, Echinoidea, and Holothuroidrea, were collected near a popular tourist destination. The average concentrations of microplastics varied among species, ranging from 8.2 to 136.5 items per individual or 0.2 to 18.1 items per gram of tissue wet weight, with 100 % occurrence. Bivalves had higher concentrations per gram of tissue compared to sediment dwellers and grazers, with Brachidontes pharaonis showing the highest levels. Actinopyga crassa, a sea cucumber, displayed the highest abundance per individual due to its large size and behavior. The identified plastic polymers suggested sources associated with tourism and maritime activities. The estimated human exposure to microplastics through bivalve consumption was minimal. Further research is needed to examine microplastics contamination in the Red Sea and its potential impacts on ecosystems and human well-being.

Protective effect of vitamin C against thiamethoxam-induced toxicity in male rats.

Background: Thiamethoxam (THM) is a neonicotinoid insecticide used to control different insect pests on fruits, vegetables, and field crops. The misuse and continuous exposure to THM cause many harmful effects on health and the reproductive system. Aim: This work aims to investigate the efficiency of vitamin C (vit C) in reducing or eliminating the harmful effects of THM on the testes, liver, and kidney of male rats. Methods: Forty-eight sexually mature male Wister albino rats (weight: 170-190 g; age: 10-11 weeks) were randomly allocated into six groups (8 males/group). The control group was orally given distilled water, vit C group was orally treated with 200 mg/kg b.wt of vit C, group 1/10 of THM LD50 orally treated with 156.3 mg/kg b.wt of THM, group 1/20 of THM LD50 orally treated with 78.15 mg/kg b.wt of THM, group 1/10 of THM LD50 + vit C orally treated with 156.3 mg/kg b.wt of THM + 200 mg/kg b.wt of vit C, and group 1/20 of THM LD50 + vit C orally treated with 78.15 mg/kg b.wt of THM + 200 mg/kg b.wt of vit C. All groups were treated for five days per week for a whole period of 58 days. Blood samples were collected at the end of the experiment, and serum was extracted for liver and kidney functions and antioxidant measurements. Reproductive organs (testis, epididymis, and seminal vesicles) were collected and weighed at the end of the experiment. Results: The results showed that groups exposed to 1/10 and 1/20 of THM LD50 significantly (p < 0.05) decreased the body weight, the reproductive organ weights (testis, epididymis, and seminal vesicles), spermatid count, sperm (count and motility), and testosterone concentration with an increase in abnormalities. In addition, the groups exposed to THM showed a decrease in protein concentration, albumin, and globulin, and caused an increase in glucose concentration. The activities of alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate transaminase (AST), creatinine, urea, and malondialdehyde (MDA) were increased while caused decrease in total antioxidant capacity (TAC) due to exposure to THM. The co-administration of vit C with HM modulated the harmful effects of the insecticide on testicular, liver, and kidney parameters, which confirmed in histopathological examination of testis. Groups orally treated with vit C showed a significant increase in spermatogenesis, spermatid numbers, and the weight of seminal vesicles. Conclusion: This study showed the importance of vit C in reducing toxic effects from exposure to THM. Accordingly, the intake of vit C by individuals who regularly handle this insecticide will be beneficial in reducing the adverse effects that may occur in the liver and kidney.

Assessing vulnerability of densely populated areas to air pollution using Sentinel-5P imageries: a case study of the Nile Delta, Egypt.

Air pollution represents one of the major environmental stressors with serious implications on human health and ecosystem health. Recently remote sensing imageries; as an alternative cost and time-effective method compared with regular monitoring techniques, were used for provision of appropriate data concerning air quality over large areas. In this context, Sentinel-5P satellite provides high-resolution images of atmospheric pollutants including nitrogen dioxide, ozone, carbon monoxide (CO) and particulate matter (PM). The current work aims to delineate vulnerability of densely populated areas in Northern-Egypt to air pollution through retrieving CO and PM2.5 from Sentinel-5P images and validate the retrieved data through simultaneous In-Situ measurements. For this purpose, our approach comprised four-step methodology; data acquisition on study area, data manipulation, validation of retrieved air quality data and mapping the vulnerability to air pollution. Based on the data retrieved from the imagery, a composite vulnerability index for each CO and PM2.5 value was developed delineating the most vulnerable areas to air pollution in the Northern Nile Delta region. Such results revealed that Sentinel-5P imagery can serve as a valuable tool for monitoring air quality and assessing vulnerability of densely populated areas to air pollution. Accordingly, it can be concluded that the applied Sentinel-5P based model can be applied effectively for other air pollutants and can be extrapolated to other areas with similar and/or different environmental settings.

Experimental, analytical, and simulation studies of modified concrete mix for radiation shielding in a mixed radiation field.

The current study assessed two concrete mixes prepared using dolomite and barite/limonite aggregates to shield against both energetic photons and neutrons. After that, a designed mix which comprised barite/goethite aggregates plus fine-powdered boron carbide additive, was proposed to improve the overall radiation shielding properties and in the same time, doesn't compromise or even improve the physic-mechanical properties of the mature concrete. The assessment started first with intensive experimental investigations to investigate the prepared mixes' shielding capabilities against both γ-rays and fast neutrons. Then, analytical computations were performed via number of reliable software programs such as; Phy-X, NXCom, MRCsC, JANIS-4, and MCNP5, in order to confirm the experimental results and to validate the created Monte-Carlo models. Finally, an intensive radiation shielding assessment for all concrete mixes understudy using, mainly, the validated MCNP models, was performed. The obtained results have revealed the superiority of barite mixes over the dolomite mix concerning attenuating photons moreover, the proposed designed mix has shown superiority over the other two prepared mixes considering shielding against; energetic photons, fast/thermal neutrons, and secondary emitted γ-rays, which nominates this mix to be a suitable universal shield that can be used even in mixed radiation fields.

New Cytotoxic Monoalkyl Glycerol Ether from the Red Sea Soft Coral Nephthea mollis.

A new monoalkyl glycerol ether, 3-(n-henicosyloxy)propane-1,2-diol (1), was isolated from the CH2 Cl2 /MeOH crude extract of the Red Sea soft coral Nephthea mollis. Additionally, three known related analogs were identified: chimyl alcohol (2), batyl alcohol (3), and 3-(icosyloxy)propane-1,2-diol (4). The chemical structure of 3-(n-henicosyloxy)propane-1,2-diol was determined using advanced spectroscopic analyses, including 1D, 2D Nuclear Magnetic Resonance (NMR), Electron Ionization mass spectra (EI-MS), and High-Resolution Electron Spray Ionization mass spectra (HR-ESI-MS) analyses. Furthermore, the identification of chimyl alcohol, batyl alcohol and 3-(icosyloxy)propane-1,2-diol was achieved by studying their EI mass fragmentation analyses and comparing their mass data with those previously reported in the literature. The cytotoxic activity of the Nephthea mollis crude extract and 3-(n-henicosyloxy)propane-1,2-diol was evaluated against five human cancer cell lines: HepG2 (hepatocellular carcinoma), MCF-7 (breast carcinoma), NCI-1299 (lung carcinoma), HeLa (cervical cancer cell), and HT-29 (colon adenocarcinoma). Moreover, 3-(n-henicosyloxy)propane-1,2-diol revealed moderate cytotoxicity against the HeLa cell lines with an IC50 value of 24.1 µM, while showing inactivity against the remaining cell lines (IC50 >100 µM).

Prevalence of multidrug-resistant and extended-spectrum ß-lactamase-producing Escherichia coli from chicken farms in Egypt.

Background and Aim: Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains exhibit antibiotic resistance and are known to infect humans worldwide. This study assessed the phenotypic and genotypic prevalence of ESBL-resistant E. coli isolates recovered from the respiratory tracts of chickens in El-Sharkia Governorate, Egypt. Materials and Methods: We obtained 250 lung samples (one lung/bird) from 50 chicken farms (5 chickens/farm) to isolate, identify, and serotype E. coli. Antimicrobial resistance susceptibility was determined using the disk diffusion method, while the ESBL phenotype was identified using double disk synergy. We detected the ß-lactamase genes, blaTEM, and blaSHV, using a polymerase chain reaction. Results: The results showed that 140/250 (56%) were infected with E. coli. All the serogroups of isolated E. coli exhibited high multi-antimicrobial resistance index values (>0.2), and 65.7% were confirmed to have ESBL. Among the isolates with the ESBL phenotypes, 55 (60%) and 32 (35%) contained the blaTEM and blaSHV genes, respectively. Conclusion: The widespread distribution of multidrug-resistant and ESBL-producing E. coli among poultry farms is a significant human health hazard. These results will help the Egyptian authorities to implement a national one-health approach to combat the antimicrobial resistance problem.

Development of a sandwich ELISA for the specific quantitation of hemagglutinin (HA)-tagged proteins during their inducible expression in Escherichia coli.

Heavy single-chain antibodies (VHH or nanobodies) are popular in the medical and analytical fields due to its small size, high solubility, stability, and other advantageous features. However, the usage of VHHs is limited by the low yield of its production and purification. In order to determine the optimal purification strategy for VHH to improve the yield, a method to monitor purification at the intermediate steps is needed. In this study, a simple, sensitive, low-cost sandwich enzyme-linked immunosorbent assay (ELISA) was developed to quantitate VHHs throughout the purification steps. Under optimized conditions, the assay has a sensitivity of 0.149 OD·mL/ng and a limit of detection (LOD) of 0.029 ng/mL. The average recoveries of the assay against the spiked samples were 101.9-106.0% and 100.7-108.0%. The method was applied to a variety of real samples for the detection of different VHHs in bacterial cell media. High amount of VHHs (up to 41.3 mg/mL), which are comparable to the average yield of VHH in standard production protocols, were detected in the media. This study raises attention to the problem of protein losses in cell culture supernatants and provides a method for the continuous detection of the protein abundance to optimize the expression and purification protocols especially for nanobodies.

A semi-empirical method for efficiency calibration of an HPGe detector against different sample densities.

In this work, a semi-empirical equation in terms of γ-energy, and sample density is derived, proposed, benchmarked, and applied for the peak efficiency calibration of an HPGe detector with respect to an axial source-to-detector configuration. The samples are in the form of cone-shaped Marinelli beakers of different densities in the range 0.7-1.6 g/cm3. The method employs the experimental measurements with the ANGLE-3 software calculations using the efficiency transfer method. The peak efficiency curve of an HPGe detector is calculated using the experimental measurements of point-like sources (133Ba, 137Cs, and 60Co). The ANGLE-3 software is then used to calculate the peak efficiency curves for samples with different densities in the γ-energy range 81-1332 keV. The peak efficiency curves are then fitted to get the energy coefficient; in addition, a linear relationship is then constructed between the energy coefficients and sample densities to get the density coefficients, and the derived equation as well. The derived equations are benchmarked using the peak efficiency curves by ANGLE-3 software in comparison with that the equation results. The results are found to be in agreement with an average relative error of about 1.5%. In addition, the derived equations are applied to estimate the activity concentration of radionuclides present in 5 cone-shaped samples with different densities using experimental measurements. The activity results are found to be in agreement with the certified values with an average relative error of about 2%. The limitation of the proposed equations is also discussed with respect to different material densities and different chemical compositions and correction factors for material composition self-attenuation for various materials are also presented.

Pre and postharvest characteristics of Dahlia pinnata var. pinnata, cav. As affected by SiO2 and CaCO3 nanoparticles under two different planting dates.

Agriculture faces many challenges because of climate changes. The nutrients present in nano-sized form improve plant productivity, especially when used at the appropriate planting time. Field experiments were conducted as a factorial experiment for evaluating two planting dates (20th September and 20th October), foliar application with nanoparticles (NPs) including silica nanoparticles (SiO2-NPs) at 1.5 and 3 mM, calcium carbonate nanoparticles (CaCO3-NPs) at 5 and 10 mM and distilled water (control) on pre- and post-harvest characteristics of Dahlia pinnata var. pinnata Cav. The results indicate that the interactions during the late planting time (20th October) and exogenous applications of SiO2-NPs at 1.5 mM or CaCO3-NPs at 10 mM have improved plant growth including plant height, stem diameter, fresh and dry weights of plant, leaf area, inflorescence diameter, inflorescence stalk length, branches number, tuber numbers, inflorescences number on the plant, and the vase life. At the same time, insignificant differences appeared in the interaction during the planting dates and SiO2 or CaCO3 -NPs concentrations on inflorescence stalk diameter, total soluble solids, membrane stability index, maximum increase in fresh weight (FW), and Si and Ca contents. In addition, all exogenous applications of NPs at the late planting time promoted the plant growth characteristics like lignin %, cellulose %, inflorescence water content, change in FW, and total water uptake. Moreover, the controls through the two planting dates recorded the maximum change in water uptake and water loss values. In short, it can be recommended to use SiO2-NPs at 1.5 mM or CaCO3-NPs at 10 mM as a foliar application at the late planting time (20th October) for obtaining the optimum quantitative and qualitative parameters of D. pinnata.

Survey and population dynamics of land snails at Sharkia Governorate, Egypt.

Land snails are a destructive agricultural pest in economic crops, but the populations for that pest are highly influenced by temperature and humidity, therefore climatic changes affected their behavior, distribution and population dynamics, for these reasons researchers should follow up on the changes in their behavior, distribution, and population dynamics. In this study, a survey study was conducted to define land snail species existing at Sharkia Governorate, Egypt, from January 2019 to March 2022 in horticultural, vegetable, and field crops and study the population dynamics for the dominant land snail species. Results showed that there are six species of land snails, these species were, Monacha cartusiana, Succinea putris, Eobania vermiculata, Theba pisana, Helicella vestalis, and Cochlicella acuta. The population dynamics of two prevalent land snail species (M. cartusiana and S. putris), were conducted during two successive growing seasons, the Egyptian clover harbored the highest number of M. cartusiana followed by wheat, while the onion was the lowest one during the study period. In the same trends, the land snail S. putris recorded the highest numbers during the growing season of the Egyptian clover crop. Finally, it is concluded that the land snail M. cartusiana considered the first grade of infestation existed everywhere at Sharkia Governorate, and the population density of the land snail is increase gradually after winter till reach the maximum density in spring.

Residue Analysis and Risk Exposure Assessment of Multiple Pesticides in Tomato and Strawberry and Their Products from Markets.

Pesticides are used on fruit and vegetable crops to obtain greater yield and quality. Residues can be detected in these crops or their products if applied pesticides do not degrade naturally. Therefore, this study aimed to estimate pesticide residues in some strawberry and tomato-based products available in the market for human consumption and associated dietary risks. Contamination with 3-15 pesticides in the tested samples was found. The total number of pesticides detected in the tested samples was 20, belonging to the group of insecticides (84%) and fungicides (16%). Pesticides of cypermethrin, thiamethoxam, chlorpyrifos, and lambda-cyhalothrin appeared at 100% in a number of samples, where the most detected was cypermethrin followed by thiamethoxam. The average values of pesticide residues detected in the tested samples ranged from 0.006 to 0.568 mg kg-1, where it was found that cypermethrin had the highest residue value and appeared in strawberry jam obtained from the market. The recovery rate of pesticides from fortified samples with pyrethroids ranged from 47.5% (fenvalerate) to 127% (lambda-cyhalothrin). Home processing of fortified tomato and strawberry samples had a significant effect on reducing residues in tomato sauce and strawberry jam, where the reduction reached 100%. The results of acute and chronic risk assessment showed that their values were much lower than 100%, indicating minimal risk of dietary intake.