RESUMEN
BACKGROUND: Literature survey proved the use of the powdered sun-dried bark and roots of Celtis africana for the treatment of cancer in South Africa. OBJECTIVE: The aim of this study was to do further isolation work on the ethyl acetate fraction and to investigate the cytotoxic activities of the various fractions and isolated compound. MATERIALS AND METHODS: Cytotoxicity of petroleum ether, chloroform, ethyl acetate, n-butanol fractions and compound 1 were tested on mouse lymphoma cell line L5178Y using the microculture tetrazolium assay. RESULTS: One new glucosphingolipid 1 was isolated from the aerial parts of C. africana. The structure of the new compound was determined by extensive analysis by one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy and mass spectrometry. The ethyl acetate fraction and compound 1 showed strong cytotoxic activity with an EC50 value of 8.3 µg/mL and 7.8 µg/mL, respectively, compared with Kahalalide F positive control (6.3 µg/mL). CONCLUSION: This is the first report of the occurrence of a cytotoxic glucosphingolipid in family Ulmaceae.
RESUMEN
Nine compounds have been isolated for the first time from Celtis africana, namely trans-N-coumaroyltyramine (1), trans-N-feruloyltyramine (2), trans-N-caffeoyltyramine (3), lauric acid (4), oleic acid (5), palmitic acid (6), lupeol (7), ß-sitosterol (8) and oleanolic acid (9), respectively. Their structures have been elucidated by different spectroscopic techniques. The isolated compounds were screened for their antioxidant, anti-inflammatory and acetylcholinestrease enzyme inhibitory activities. Compounds 1-3 showed significant antioxidant and anti-inflammatory activities and weak to moderate acetylcholinestrease enzyme inhibition activity.
Asunto(s)
Cannabaceae/química , Inhibidores de la Colinesterasa/aislamiento & purificación , Depuradores de Radicales Libres/aislamiento & purificación , Fenoles/aislamiento & purificación , Componentes Aéreos de las Plantas/química , Extractos Vegetales/aislamiento & purificación , Aminas/aislamiento & purificación , Aminas/farmacología , Animales , Carragenina , Inhibidores de la Colinesterasa/química , Inhibidores de la Colinesterasa/farmacología , Edema/inducido químicamente , Edema/tratamiento farmacológico , Ácidos Grasos/química , Ácidos Grasos/aislamiento & purificación , Ácidos Grasos/farmacología , Femenino , Depuradores de Radicales Libres/química , Depuradores de Radicales Libres/farmacología , Masculino , Ácido Oleanólico/química , Ácido Oleanólico/aislamiento & purificación , Ácido Oleanólico/farmacología , Triterpenos Pentacíclicos/química , Triterpenos Pentacíclicos/aislamiento & purificación , Triterpenos Pentacíclicos/farmacología , Fenoles/farmacología , Extractos Vegetales/química , Extractos Vegetales/farmacología , Ratas , Ratas Wistar , Sitoesteroles/química , Sitoesteroles/aislamiento & purificación , Sitoesteroles/farmacologíaRESUMEN
Two new C-glycosylflavonoids celtisides A (1) and B (2) have been isolated from n-butanol-soluble fraction of Celtis africana, along with five known C-glycosylflavonoids vitexin (3), orientin (4), isoswertiajaponin (5), isoswertisin (6), and 2â³-O-rhamnosyl vitexin (7) reported for the first time from this species. Their structures were assigned from 1D and 2D NMR spectra. These compounds were investigated for biological activities and showed significant antioxidant and urease inhibitory activities.