RESUMEN
Time-course of induced accumulation of callose in tomato cells has been studied. Localization of callose in L. esculenthum cells was investigated by fluorescent microscopy technique, and the optimal time for its determination was found. Callose accumulation in tomato cells treated with different biotic elicitors was determined. Nonlinear dependence between callose accumulation and concentration of chitin oligomers (with 3-5 N-acetylglucosamine fragments) was established. Increasing of callose accumulation in tomato cells was proportional to the increase of concentration ofchitin dimer and chitosan in the culture medium.
Asunto(s)
Acetilglucosamina/farmacología , Membrana Celular/metabolismo , Quitosano/farmacología , Glucanos/metabolismo , Solanum lycopersicum/efectos de los fármacos , Solanum lycopersicum/citología , Solanum lycopersicum/genética , Solanum lycopersicum/metabolismo , Microscopía Fluorescente , Factores de TiempoRESUMEN
The levels of chitinase activity induced with elicitors in tomato cells have been detected. It was shown that enzymatic activity depended on degree of polymerization and concentration of biotic elicitors.
