RESUMEN
The ability of animals to perceive and respond to sensory information is essential for their survival in diverse environments. While much progress has been made in understanding various sensory modalities, the sense of hygrosensation, which involves the detection and response to humidity, remains poorly understood. In this study, we focused on the hygrosensory, and closely related thermosensory, systems in the vinegar fly Drosophila melanogaster to unravel the molecular profile of the cells of these senses. Using a transcriptomic analysis of over 37,000 nuclei, we identified twelve distinct clusters of cells corresponding to temperature-sensing arista neurons, humidity-sensing sacculus neurons, and support cells relating to these neurons. By examining the expression of known and novel marker genes, we validated the identity of these clusters and characterized their gene expression profiles. We found that each cell type could be characterized by a unique expression profile of ion channels, GPCR signaling molecules, synaptic vesicle cycle proteins, and cell adhesion molecules. Our findings provide valuable insights into the molecular basis of hygro- and thermosensation. Understanding the mechanisms underlying hygro- and thermosensation may shed light on the broader understanding of sensory systems and their adaptation to different environmental conditions in animals.
Asunto(s)
Ascomicetos , Drosophilidae , Animales , Drosophila melanogaster/genética , Ácido Acético , Neuronas , Núcleo CelularRESUMEN
Although the vinegar fly Drosophila melanogaster is arguably the most studied organism on the planet, fundamental aspects of this species' natural ecology have remained enigmatic [1]. We have here investigated a wild population of D. melanogaster from a mopane forest in Zimbabwe. We find that these flies are closely associated with marula fruit (Sclerocarya birrea) and propose that this seasonally abundant and predominantly Southern African fruit is a key ancestral host of D. melanogaster. Moreover, when fruiting, marula is nearly exclusively used by D. melanogaster, suggesting that these forest-dwelling D. melanogaster are seasonal specialists, in a similar manner to, e.g., Drosophila erecta on screw pine cones [2]. We further demonstrate that the main chemicals released by marula activate odorant receptors that mediate species-specific host choice (Or22a) [3, 4] and oviposition site selection (Or19a) [5]. The Or22a-expressing neurons-ab3A-respond strongly to the marula ester ethyl isovalerate, a volatile rarely encountered in high amounts in other fruit. We also show that Or22a differs among African populations sampled from a wide range of habitats, in line with a function associated with host fruit usage. Flies from Southern Africa, most of which carry a distinct allele at the Or22a/Or22b locus, have ab3A neurons that are more sensitive to ethyl isovalerate than, e.g., European flies. Finally, we discuss the possibility that marula, which is also a culturally and nutritionally important resource to humans, may have helped the transition to commensalism in D. melanogaster.
Asunto(s)
Anacardiaceae/química , Quimiotaxis , Drosophila melanogaster/fisiología , Frutas/química , Receptores Odorantes/fisiología , África Austral , Animales , Conducta Alimentaria , Femenino , Estaciones del AñoRESUMEN
Humidity is an omnipresent climatic factor that influences the fitness, reproductive behavior and geographic distribution of animals. Insects in particular use humidity cues to navigate the environment. Although the sensory neurons of this elusive sense were first described more than fifty years ago, the transduction mechanism of humidity sensing (hygrosensation) remains unknown. Recent work has uncovered some of the key molecules involved, opening up for novel approaches to study hygrosensory transduction. In this review, I will discuss this progress made toward understanding hygrosensation in insects.
Asunto(s)
Humedad , Proteínas de Insectos/metabolismo , Insectos/fisiología , Transducción de Señal , Animales , Sensación , Células Receptoras Sensoriales/fisiologíaRESUMEN
The Drosophila antenna contains receptor neurons for mechanical, olfactory, thermal, and humidity stimuli. Neurons expressing the ionotropic receptor IR40a have been implicated in the selection of an appropriate humidity range [1, 2], but although previous work indicates that insect hygroreceptors may be made up by a "triad" of neurons (with a dry-, a cold-, and a humid-air-responding cell [3]), IR40a expression included only cold- and dry-air cells. Here, we report the identification of the humid-responding neuron that completes the hygrosensory triad in the Drosophila antenna. This cell type expresses the Ir68a gene, and Ir68a mutation perturbs humidity preference. Next, we follow the projections of Ir68a neurons to the brain and show that they form a distinct glomerulus in the posterior antennal lobe (PAL). In the PAL, a simple sensory map represents related features of the external environment with adjacent "hot," "cold," "dry," and "humid" glomeruli-an organization that allows for both unique and combinatorial sampling by central relay neurons. Indeed, flies avoided dry heat more robustly than humid heat, and this modulation was abolished by silencing of dry-air receptors. Consistently, at least one projection neuron type received direct synaptic input from both temperature and dry-air glomeruli. Our results further our understanding of humidity sensing in the Drosophila antenna, uncover a neuronal substrate for early sensory integration of temperature and humidity in the brain, and illustrate the logic of how ethologically relevant combinations of sensory cues can be processed together to produce adaptive behavioral responses.
Asunto(s)
Drosophila melanogaster/fisiología , Sensación Térmica , Animales , Encéfalo/fisiología , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Humedad , TemperaturaRESUMEN
When activating muscles, motor neurons in the spinal cord also activate Renshaw cells, which provide recurrent inhibitory feedback to the motor neurons. The tight coupling with motor neurons suggests that Renshaw cells have an integral role in movement, a role that is yet to be elucidated. Here we used the selective expression of the nicotinic cholinergic receptor α2 (Chrna2) in mice to genetically target the vesicular inhibitory amino acid transporter (VIAAT) in Renshaw cells. Loss of VIAAT from Chrna2Cre -expressing Renshaw cells did not impact any aspect of drug-induced fictive locomotion in the neonatal mouse or change gait, motor coordination, or grip strength in adult mice of both sexes. However, motor neurons from neonatal mice lacking VIAAT in Renshaw cells received spontaneous inhibitory synaptic input with a reduced frequency, showed lower input resistance, and had an increased number of proprioceptive glutamatergic and calbindin-labeled putative Renshaw cell synapses on their soma and proximal dendrites. Concomitantly, Renshaw cells developed with increased excitability and a normal number of cholinergic motor neuron synapses, indicating a compensatory mechanism within the recurrent inhibitory feedback circuit. Our data suggest an integral role for Renshaw cell signaling in shaping the excitability and synaptic input to motor neurons.SIGNIFICANCE STATEMENT We here provide a deeper understanding of spinal cord circuit formation and the repercussions for the possible role for Renshaw cells in speed and force control. Our results suggest that while Renshaw cells are not directly required as an integral part of the locomotor coordination machinery, the development of their electrophysiological character is dependent on vesicular inhibitory amino acid transporter-mediated signaling. Further, Renshaw cell signaling is closely associated with the molding of motor neuron character proposing the existence of a concerted maturation process, which seems to endow this particular spinal cord circuit with the plasticity to compensate for loss of the Renshaw cell in adult circuit function.
Asunto(s)
Envejecimiento/fisiología , Retroalimentación Fisiológica/fisiología , Neuronas Motoras/fisiología , Inhibición Neural/fisiología , Células de Renshaw/fisiología , Transmisión Sináptica/fisiología , Adaptación Fisiológica/fisiología , Animales , Células Cultivadas , Femenino , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Vías Nerviosas/fisiología , Plasticidad Neuronal/fisiología , Sinapsis/fisiologíaRESUMEN
Feces is an abundant, rich source of energy, utilized by a myriad of organisms, not least by members of the order Diptera, i.e., flies. How Drosophila melanogaster reacts to fecal matter remains unclear. Here, we examined oviposition behavior toward a range of fecal samples from mammals native to the putative Southeast African homeland of the fly. We show that D. melanogaster display a strong oviposition aversion toward feces from carnivorous mammals but indifference or even attraction toward herbivore dung. We identify a set of four predictor volatiles, which can be used to differentiate fecal from non-fecal matter, as well as separate carnivore from herbivore feces. Of these volatiles, phenol-indicative of carnivore feces-confers egg-laying aversion and is detected by a single class of sensory neurons expressing Or46a. The Or46a-expressing neurons are necessary and sufficient for oviposition site aversion. We further demonstrate that carnivore feces-unlike herbivore dung-contain a high rate of pathogenic bacteria taxa. These harmful bacteria produce phenol from L-tyrosine, an amino acid specifically enriched in high protein diets, such as consumed by carnivores. Finally, we demonstrate that carnivore feces, as well as phenol, is also avoided by a ball-rolling species of dung beetle, suggesting that phenol is a widespread avoidance signal because of its association with pathogenic bacteria.
Asunto(s)
Reacción de Prevención , Drosophila melanogaster/fisiología , Heces/química , Oviposición , Fenoles/metabolismo , Animales , Dieta , Femenino , Mamíferos/fisiología , Células Receptoras Sensoriales/metabolismoRESUMEN
Environmental humidity influences the fitness and geographic distribution of all animals [1]. Insects in particular use humidity cues to navigate the environment, and previous work suggests the existence of specific sensory mechanisms to detect favorable humidity ranges [2-5]. Yet, the molecular and cellular basis of humidity sensing (hygrosensation) remains poorly understood. Here we describe genes and neurons necessary for hygrosensation in the vinegar fly Drosophila melanogaster. We find that members of the Drosophila genus display species-specific humidity preferences related to conditions in their native habitats. Using a simple behavioral assay, we find that the ionotropic receptors IR40a, IR93a, and IR25a are all required for humidity preference in D. melanogaster. Yet, whereas IR40a is selectively required for hygrosensory responses, IR93a and IR25a mediate both humidity and temperature preference. Consistent with this, the expression of IR93a and IR25a includes thermosensory neurons of the arista. In contrast, IR40a is excluded from the arista but is expressed (and required) in specialized neurons innervating pore-less sensilla of the sacculus, a unique invagination of the third antennal segment. Indeed, calcium imaging showed that IR40a neurons directly respond to changes in humidity, and IR40a knockdown or IR93a mutation reduced their responses to stimuli. Taken together, our results suggest that the preference for a specific humidity range depends on specialized sacculus neurons, and that the processing of environmental humidity can happen largely in parallel to that of temperature.
Asunto(s)
Proteínas de Drosophila/genética , Drosophila melanogaster/fisiología , Humedad , Receptores Ionotrópicos de Glutamato/genética , Sensación , Animales , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/genética , Masculino , Neuronas/metabolismo , Receptores Ionotrópicos de Glutamato/metabolismo , Sensilos/metabolismoRESUMEN
Alarm pheromones are important semiochemicals used by many animal species to alert conspecifics or other related species of impending danger. In this review, we describe recent developments in our understanding of the neural mechanisms underlying the ability of fruit flies, zebrafish and mice to mediate the detection of alarm pheromones. Specifically, alarm pheromones are detected in these species through specialized olfactory subsystems that are unique to the chemosensitive receptors, second messenger-signaling and physiology. Thus, the alarm pheromones appears to be detected by signaling mechanisms that are distinct from those seen in the canonical olfactory system.
Asunto(s)
Comunicación Animal , Células Quimiorreceptoras/diagnóstico por imagen , Células Quimiorreceptoras/metabolismo , Feromonas/fisiología , Sistemas de Mensajero Secundario , Animales , Percepción Olfatoria , Olfato , UltrasonografíaRESUMEN
Motor neurons become hyperexcitable during progression of amyotrophic lateral sclerosis (ALS). This abnormal firing behavior has been explained by changes in their membrane properties, but more recently it has been suggested that changes in premotor circuits may also contribute to this abnormal activity. The specific circuits that may be altered during development of ALS have not been investigated. Here we examined the Renshaw cell recurrent circuit that exerts inhibitory feedback control on motor neuron firing. Using two markers for Renshaw cells (calbindin and cholinergic nicotinic receptor subunit alpha2 [Chrna2]), two general markers for motor neurons (NeuN and vesicular acethylcholine transporter [VAChT]), and two markers for fast motor neurons (Chondrolectin and calcitonin-related polypeptide alpha [Calca]), we analyzed the survival and connectivity of these cells during disease progression in the Sod1(G93A) mouse model. Most calbindin-immunoreactive (IR) Renshaw cells survive to end stage but downregulate postsynaptic Chrna2 in presymptomatic animals. In motor neurons, some markers are downregulated early (NeuN, VAChT, Chondrolectin) and others at end stage (Calca). Early downregulation of presynaptic VAChT and Chrna2 was correlated with disconnection from Renshaw cells as well as major structural abnormalities of motor axon synapses inside the spinal cord. Renshaw cell synapses on motor neurons underwent more complex changes, including transitional sprouting preferentially over remaining NeuN-IR motor neurons. We conclude that the loss of presynaptic motor axon input on Renshaw cells occurs at early stages of ALS and disconnects the recurrent inhibitory circuit, presumably resulting in diminished control of motor neuron firing. J. Comp. Neurol. 521:1449-1469, 2013. © 2012 Wiley Periodicals, Inc.
RESUMEN
The vast diversity of GABAergic interneurons is believed to endow hippocampal microcircuits with the required flexibility for memory encoding and retrieval. However, dissection of the functional roles of defined interneuron types has been hampered by the lack of cell-specific tools. We identified a precise molecular marker for a population of hippocampal GABAergic interneurons known as oriens lacunosum-moleculare (OLM) cells. By combining transgenic mice and optogenetic tools, we found that OLM cells are important for gating the information flow in CA1, facilitating the transmission of intrahippocampal information (from CA3) while reducing the influence of extrahippocampal inputs (from the entorhinal cortex). Furthermore, we found that OLM cells were interconnected by gap junctions, received direct cholinergic inputs from subcortical afferents and accounted for the effect of nicotine on synaptic plasticity of the Schaffer collateral pathway. Our results suggest that acetylcholine acting through OLM cells can control the mnemonic processes executed by the hippocampus.
Asunto(s)
Región CA1 Hipocampal/citología , Región CA3 Hipocampal/citología , Interneuronas/fisiología , Red Nerviosa/fisiología , Neuronas/fisiología , 6-Ciano 7-nitroquinoxalina 2,3-diona/farmacología , Animales , Animales Recién Nacidos , Estimulación Eléctrica , Antagonistas de Aminoácidos Excitadores/farmacología , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Potenciales Postsinápticos Excitadores/genética , Antagonistas del GABA/farmacología , Proteínas Fluorescentes Verdes , Técnicas In Vitro , Interneuronas/efectos de los fármacos , Potenciación a Largo Plazo/efectos de los fármacos , Potenciación a Largo Plazo/genética , Proteínas Luminiscentes/genética , Proteínas Luminiscentes/metabolismo , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Neuronas/clasificación , Nicotina/farmacología , Agonistas Nicotínicos/farmacología , Técnicas de Placa-Clamp , Picrotoxina/farmacología , Lectinas de Plantas/genética , Lectinas de Plantas/metabolismo , ARN Mensajero/metabolismo , Receptores Nicotínicos/genética , Valina/análogos & derivados , Valina/farmacología , Imagen de Colorante Sensible al Voltaje , Ácido gamma-Aminobutírico/metabolismoRESUMEN
Gamma motor neurons (MNs), the efferent component of the fusimotor system, regulate muscle spindle sensitivity. Muscle spindle sensory feedback is required for proprioception that includes sensing the relative position of neighboring body parts and appropriately adjust the employed strength in a movement. The lack of a single and specific genetic marker has long hampered functional and developmental studies of gamma MNs. Here we show that the serotonin receptor 1d (5-ht1d) is specifically expressed by gamma MNs and proprioceptive sensory neurons. Using mice expressing GFP driven by the 5-ht1d promotor, we performed whole-cell patch-clamp recordings of 5-ht1d::GFP⺠and 5-ht1d::GFP⻠motor neurons from young mice. Hierarchal clustering analysis revealed that gamma MNs have distinct electrophysiological properties intermediate to fast-like and slow-like alpha MNs. Moreover, mice lacking 5-ht1d displayed lower monosynaptic reflex amplitudes suggesting a reduced response to sensory stimulation in motor neurons. Interestingly, adult 5-ht1d knockout mice also displayed improved coordination skills on a beam-walking task, implying that reduced activation of MNs by Ia afferents during provoked movement tasks could reduce undesired exaggerated muscle output. In summary, we show that 5-ht1d is a novel marker for gamma MNs and that the 5-ht1d receptor is important for the ability of proprioceptive circuits to receive and relay accurate sensory information in developing and mature spinal cord motor circuits.
Asunto(s)
Retroalimentación Sensorial/fisiología , Neuronas Motoras gamma/fisiología , Husos Musculares/fisiología , Neuronas Aferentes/fisiología , Receptor de Serotonina 5-HT1D/fisiología , Animales , Ratones , Ratones Noqueados , Neuronas Motoras gamma/citología , Receptor de Serotonina 5-HT1D/análisis , Serotonina/fisiología , Médula Espinal/citología , Médula Espinal/fisiologíaRESUMEN
Spinal cholinergic neurons are critical for motor function in both the autonomic and somatic nervous systems and are affected in spinal cord injury and in diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy. Using two screening approaches and in situ hybridization, we identified 159 genes expressed in typical cholinergic patterns in the spinal cord. These include two general cholinergic neuron markers, one gene exclusively expressed in motor neurons, and nine genes expressed in unknown subtypes of somatic motor neurons. Further, we present evidence that chondrolectin (Chodl) is expressed by fast motor neurons and that estrogen-related receptor beta (ERRbeta) is a candidate marker for slow motor neurons. In addition, we suggest paired-like homeodomain transcription factor 2 (Pitx2) as a marker for cholinergic partition cells.
Asunto(s)
Proteínas de Homeodominio/metabolismo , Lectinas Tipo C/metabolismo , Neuronas Motoras/fisiología , Receptores de Estrógenos/metabolismo , Médula Espinal/fisiología , Factores de Transcripción/metabolismo , Animales , Técnica del Anticuerpo Fluorescente , Proteínas de Homeodominio/genética , Hibridación in Situ , Lectinas Tipo C/genética , Ratones , Ratones Endogámicos C57BL , Neuronas Motoras/citología , Análisis de Secuencia por Matrices de Oligonucleótidos , Técnicas de Placa-Clamp , Receptores de Estrógenos/genética , Médula Espinal/citología , Factores de Transcripción/genética , Proteína del Homeodomínio PITX2RESUMEN
Glutamate-induced excitotoxicity has been suggested to influence pathogenesis in amyotrophic lateral sclerosis (ALS). Vesicular glutamate transporters (VGLUTs) are responsible for transport of glutamate into synaptic vesicles. Nerve terminals that envelop motor neurons in the spinal cord contain VGLUT2 and are likely responsible for most glutamate release on motor neurons. The role of VGLUT2 in ALS and its potential role to influence motor neuron survival have not previously been studied. Here, in a mouse model of ALS, we show that genetic reduction of VGLUT2 protein levels rescues motor neurons in the lumbar spinal cord and in the brainstem as well as neuromuscular junctions in tibialis anterior. Although the number of remaining motor neurons increased, neither disease onset nor life span was affected. We also show that the motor neuron subpopulation-specific markers calcitonin/calcitonin-related polypeptide alpha (Calca) and estrogen related receptor beta (ERRbeta) respond in a similar way to reduced VGLUT2 as the whole motor neuron population suggesting that the rescued motor neurons are not of a particular motor unit type. Taken together, this suggests that reduced levels of VGLUT2 decrease motor neuron degeneration but do not prevent loss of motor neuron function in the SOD1(G93A) mouse model for ALS.
Asunto(s)
Neuronas Motoras/fisiología , Degeneración Nerviosa/fisiopatología , Superóxido Dismutasa/metabolismo , Proteína 2 de Transporte Vesicular de Glutamato/metabolismo , Esclerosis Amiotrófica Lateral/fisiopatología , Animales , Tronco Encefálico/fisiopatología , Calcitonina/metabolismo , Péptido Relacionado con Gen de Calcitonina , Supervivencia Celular/fisiología , Modelos Animales de Enfermedad , Receptor beta de Estrógeno/metabolismo , Femenino , Humanos , Masculino , Ratones , Ratones Transgénicos , Músculo Esquelético/inervación , Músculo Esquelético/fisiopatología , Unión Neuromuscular/fisiopatología , Precursores de Proteínas/metabolismo , Médula Espinal/fisiopatología , Superóxido Dismutasa/genética , Proteína 2 de Transporte Vesicular de Glutamato/genéticaRESUMEN
Neuronal network formation in the developing nervous system is dependent on the accurate navigation of nerve cell axons and dendrites, which is controlled by attractive and repulsive guidance cues. Ephrins and their cognate Eph receptors mediate many repulsive axonal guidance decisions by intercellular interactions resulting in growth cone collapse and axon retraction of the Eph-presenting neuron. We show that the Rac-specific GTPase-activating protein alpha2-chimaerin binds activated EphA4 and mediates EphA4-triggered axonal growth cone collapse. alpha-Chimaerin mutant mice display a phenotype similar to that of EphA4 mutant mice, including aberrant midline axon guidance and defective spinal cord central pattern generator activity. Our results reveal an alpha-chimaerin-dependent signaling pathway downstream of EphA4, which is essential for axon guidance decisions and neuronal circuit formation in vivo.
Asunto(s)
Diferenciación Celular/genética , Sistema Nervioso Central/anomalías , Sistema Nervioso Central/metabolismo , Quimerina 1/metabolismo , Conos de Crecimiento/metabolismo , Receptor EphA4/metabolismo , Animales , Animales Recién Nacidos , Encéfalo/anomalías , Encéfalo/metabolismo , Encéfalo/fisiopatología , Comunicación Celular/genética , Células Cultivadas , Sistema Nervioso Central/citología , Quimerina 1/genética , Regulación hacia Abajo/genética , Trastornos Neurológicos de la Marcha/genética , Trastornos Neurológicos de la Marcha/metabolismo , Trastornos Neurológicos de la Marcha/fisiopatología , Regulación del Desarrollo de la Expresión Génica/genética , Conos de Crecimiento/ultraestructura , Ratones , Ratones Noqueados , Vías Nerviosas/anomalías , Vías Nerviosas/metabolismo , Vías Nerviosas/fisiopatología , Unión Proteica/genética , Tractos Piramidales/anomalías , Tractos Piramidales/metabolismo , Tractos Piramidales/fisiopatología , Transducción de Señal/genética , Médula Espinal/anomalías , Médula Espinal/citología , Médula Espinal/metabolismoRESUMEN
Glutamatergic excitatory neurotransmission is dependent on glutamate release from presynaptic vesicles loaded by three members of the solute carrier family, Slc17a6-8, which function as vesicular glutamate transporters (VGLUTs). Here, we show that VGLUT2 (Slc17a6) is required for life ex utero. Vglut2 null mutant mice die immediately after birth because of the absence of respiratory behavior. Investigations at embryonic stages revealed that neural circuits in the location of the pre-Bötzinger (PBC) inspiratory rhythm generator failed to become active. However, neurons with bursting pacemaker properties and anatomical integrity of the PBC area were preserved. Vesicles at asymmetric synapses were fewer and malformed in the Vglut2 null mutant hindbrain, probably causing the complete disruption of AMPA/kainate receptor-mediated synaptic activity in mutant PBC cells. The functional deficit results from an inability of PBC neurons to achieve synchronous activation. In contrast to respiratory rhythm generation, the locomotor central pattern generator of Vglut2 null mutant mice displayed normal rhythmic and coordinated activity, suggesting differences in their operating principles. Hence, the present study identifies VGLUT2-mediated signaling as an obligatory component of the developing respiratory rhythm generator.
Asunto(s)
Tipificación del Cuerpo , Actividad Motora/fisiología , Neuronas Motoras/fisiología , Centro Respiratorio/citología , Mecánica Respiratoria/fisiología , Proteína 2 de Transporte Vesicular de Glutamato/fisiología , Animales , Animales Recién Nacidos , Bicuculina/farmacología , Embrión de Mamíferos , Antagonistas del GABA/farmacología , Regulación del Desarrollo de la Expresión Génica/fisiología , Glicinérgicos/farmacología , Inmunohistoquímica/métodos , Hibridación in Situ/métodos , Técnicas In Vitro , Potenciales de la Membrana/efectos de los fármacos , Potenciales de la Membrana/genética , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Microscopía Electrónica de Transmisión/métodos , Neuronas Motoras/efectos de los fármacos , Neuronas Motoras/ultraestructura , Periodicidad , Receptores de Neuroquinina-1/metabolismo , Mecánica Respiratoria/genética , Médula Espinal/fisiología , Estricnina/farmacología , Tirosina 3-Monooxigenasa/metabolismo , Proteína 2 de Transporte Vesicular de Glutamato/deficienciaRESUMEN
Central pattern generators (CPGs) are defined as neuronal circuits capable of producing a rhythmic and coordinated output without the influence of sensory input. The locomotor and respiratory neuronal circuits are two of the better-characterized CPGs, although much work remains to fully understand how these networks operate. Glutamatergic neurons are involved in most neuronal circuits of the nervous system and considerable efforts have been made to study glutamate receptors in nervous system signaling using a variety of approaches. Because of the complexity of glutamate-mediated signaling and the variety of receptors triggered by glutamate, it has been difficult to pinpoint the role of glutamatergic neurons in neuronal circuits. In addition, glutamate is an amino acid used by every cell, which has hampered identification of glutamatergic neurons. Glutamatergic excitatory neurotransmission is dependent on the release from glutamate-filled presynaptic vesicles loaded by three members of the solute carrier family, Slc17a6-8, which function as vesicular glutamate transporters (VGLUTs). Recent data describe that Vglut2 (Slc17a6) null mutant mice die immediately after birth due to a complete loss of the stable autonomous respiratory rhythm generated by the pre-Bötzinger complex. Surprisingly, we found that basal rhythmic locomotor activity is not affected in Vglut2 null mutant embryos. With this perspective, we discuss data regarding presence of VGLUT1, VGLUT2 and VGLUT3 positive neuronal populations in the spinal cord.