A Comparative Study of Methods for Detecting Extraterrestrial Life in Exploration Missions to Mars and the Solar System II: Targeted Characteristics, Detection Techniques, and Their Combination for Survey, Detection, and Analysis.

We present a comparative study of the methods used in the search for extraterrestrial microorganism life, including a summary table where different life-detection techniques can be easily compared as an aid to mission and instrument design aimed at life detection. This is an extension of previous study, where detection techniques for a series of target characteristics and molecules that could constitute a positive life detection were evaluated. This comparison has been extended with a particular consideration to sources of false positives, the causes of negative detection, the results of detection techniques when presented regarding terrestrial life, and additional science objectives that could be achieved outside the primary aim of detecting life. These additions address both the scientific and programmatic side of exploration mission design, where a successful proposal must demonstrate probable outcomes and be able to return valuable results even if no life is found. The applicability of the life detection techniques is considered for Earth life, Earth-independent life (life emerging independently from that on Earth,) and Earth-kin life (sharing a common ancestor with life on Earth), and techniques effective in detecting Earth life should also be useful in the detection of Earth-kin life. However, their applicability is not guaranteed for Earth-independent life. As found in our previous study, there exists no realistic single detection method that can conclusively determine the discovery of extraterrestrial life, and no method is superior to all others. In this study, we further consider combinations of detection techniques and identify imaging as a valuable addition to molecule detection methods, even in cases where there is insufficient resolution to observe the detailed morphology of a microbial cell. The search for extraterrestrial life is further divided into a survey-and-detection and analysis-and-conclusion step. These steps benefit from different detection techniques, but imaging is necessary for both parts.

Comparative study of methods for detecting extraterrestrial life in exploration mission of Mars and the solar system.

The detection and analysis of extraterrestrial life are important issues of space science. Mars is among the most important planets to explore for extraterrestrial life, owing both to its physical properties and to its ancient and present environments as revealed by previous exploration missions. In this paper, we present a comparative study of methods for detecting extraterrestrial life and life-related substances. To this end, we have classified and summarized the characteristics targeted for the detection of extraterrestrial life in solar system exploration mission and the methods used to evaluate them. A summary table is presented. We conclude that at this moment (i) there is no realistic single detection method capable of concluding the discovery of extraterrestrial life, (ii) no single method has an advantage over the others in all respects, and (iii) there is no single method capable of distinguishing extraterrestrial life from terrestrial life. Therefore, a combination of complementary methods is essential. We emphasize the importance of endeavoring to detect extraterrestrial life without overlooking possible alien life forms, even at the cost of tolerating false positives. Summaries of both the targets and the detection methods should be updated continuously, and comparative studies of both should be pursued. Although this study assumes Mars to be a model site for the primary environment for life searches, both the targets and detection methods described herein will also be useful for searching for extraterrestrial life in any celestial environment and for the initial inspection of returned samples.

In situ biochemical characterization of Venus cloud particles using a life-signature detection microscope.

Much of the information about the size and shape of aerosols forming haze and the cloud layer of Venus is obtained from indirect inferences from nephelometers on probes and from the analysis of the variation of polarization with the phase angle and the glory feature from images of Venus. The microscopic imaging of Venus' aerosols has recently been advocated. Direct measurements from a fluorescence microscope can provide information on the morphology, density, and biochemical characteristics of the particles; thus, fluorescence microscopy is attractive for in situ particle characterization of the Venus cloud layer. Fluorescence imaging of Venus cloud particles presents several challenges owing to the sulfuric acid composition and corrosive effects. In this article, we identify the challenges and describe our approach to overcoming them for a fluorescence microscope based on an in situ biochemical and physical characterization instrument for use in the clouds of Venus from a suitable aerial platform. We report that pH adjustment using alkali was effective for obtaining fluorescence images and that fluorescence attenuation was observed after the adjustment, even when the acidophile suspension in concentrated sulfuric acid was used as a sample.

Laboratory experiment of ATP measurement using Mars soil simulant: as a method for extraterrestrial life detection.

We present a laboratory experiment of ATP measurement using Mars soil simulant and Escherichia coli (E. coli) with a point of view for future application to searching extraterrestrial life. We used Mars Global Simulant MGS-1 (Exolith Lab) as soil simulant, added E. coli suspension to it, then the soil simulant with E. coli was dried. Various configurations of samples with different E. coli density, 1.75 × 102, 1.75 × 103, 1.75 × 104, 1.75 × 105, and 1.75 × 106 cells (g soil)-1, were prepared together with controls. ATP extraction reagent and luminescence reagent were added to the sample, and bioluminescence measurement was performed. The result suggests significant detection of ATP for samples with E. coli density used in this work. Similar experiments but without the soil simulant were carried out, and results with and without the soil simulant are compared. Based on the ATP measurement studied in this work, we discussed extraterrestrial life search, planetary protection, relation with the panspermia hypothesis, and also other applications.

Design for stray-light reduction to a Martian ionospheric imager.

We demonstrate a new design high-contrast optics suitable for use on a stray-light reduction. Two-dimensional distribution of diffracted light was numerically analyzed and confirmed by a laboratory measurement to determine its characteristics. Laboratory measurements confirm stray-light performance with ∼10-8 rejection viewing as close as 1.0° to a bright-light source. The present improvement replaces the conventional normal-edge vane by an edge with microscopic Gaussian-shaped structures. This permits simplified fabrication, provides even more weight reduction, and delivers improved performance for a given size.

Laboratory demonstration of a cryogenic deformable mirror for wavefront correction of space-borne infrared telescopes.

This paper demonstrates a cryogenic deformable mirror (DM) with 1020 actuators based on micro-electrical mechanical systems (MEMS) technology. Cryogenic space-borne infrared telescopes can experience a wavefront error due to a figure error of their mirror surface, which makes the imaging performance worse. For on-orbit wavefront correction as one solution, we developed a MEMS-processed electro-static DM with a special surrounding structure for use under the cryogenic temperature. We conducted a laboratory demonstration of its operation in three cooling cycles between 5 K and 295 K. Using a laser interferometer, we detected the deformation corresponding to the applied voltages under the cryogenic temperature for the first time. The relationship between voltages and displacements was qualitatively expressed by the quadratic function, which is assumed based on the principle of electro-static DMs. We also found that it had a high operating repeatability of a few nm root-mean-square and no significant hysteresis. Using the measured values of repeatability, we simulated the improvement of the point spread function (PSF) by wavefront correction with our DM. These results show that our developed DM is effective in improving imaging performance and PSF contrast of space-borne infrared telescopes.

Quality evaluation of spaceborne SiC mirrors (II): evaluation technology for mirror accuracy using actual measurement data of samples cut out from a mirror surface.

The authors studied the quality evaluation technology of a spaceborne large-scale lightweight mirror that was made of silicon carbide (SiC)-based material. To correlate the material property of a mirror body and the mirror accuracy, the authors evaluated the mirror surface deviation of a prototype mirror by inputting actually measured coefficient of thermal expansion (CTE) data into a finite element analysis model. The CTE data were obtained by thermodilatometry using a commercial grade thermal dilatometer for the samples cut from all over the mirror surface. The computationally simulated contour diagrams well reproduced the mirror accuracy profile that the actual mirror showed in cryogenic testing. Density data were also useful for evaluating the mirror surface deviation because they had a close relationship with the CTE.

Quality evaluation of spaceborne SiC mirrors (I): analytical examination of the effects on mirror accuracy by variation in the thermal expansion property of the mirror surface.

The Japan Aerospace Exploration Agency has studied a large-scale lightweight mirror constructed of reaction-bonded silicon carbide-based material as a key technology in future astronomical and earth observation missions. The authors selected silicon carbide as the promising candidate due to excellent characteristics of specific stiffness and thermal stability. One of the most important technical issues for large-scale ceramic components is the uniformity of the material's property, depending on part and processing. It might influence mirror accuracy due to uneven thermal deformation. The authors conducted systematic case studies for the conditions of CTE by finite element analysis to know the typical influence of material property nonuniformity on mirror accuracy and consequently derived a comprehensive empirical equation for the series of CTE's main factors. In addition, the authors computationally reproduced the mirror accuracy profile of a small prototype mirror shown in cryogenic testing and hereby verified wide-range practical computational evaluation technology of mirror accuracy.

Cryogenic optical testing of an 800 mm lightweight C/SiC composite mirror mounted on a C/SiC optical bench.

We tested the optical performance at cryogenic temperatures of an 800 mm diameter lightweight mirror, consisting of carbon-fiber reinforced silicon carbide and with a mass of 11.2 kg. The ceramic composite of the mirror was HB-Cesic, developed by ECM, Germany, and Mitsubishi Electric Corporation, Japan. The test was carried out while the mirror was mounted, via Invar stress relief supports, on a lightweight optical bench also made of HB-Cesic. During the test, both the mirror and the optical bench were cooled to 18 K in a liquid-helium chamber. The test consisted of measuring the mirror's change of surface figure with an interferometer installed outside the cryo-chamber. The cryogenic deformation of the mirror was 110 nm RMS with no significant residual deformation after cooling, which is very promising for the applicability of the HB-Cesic composite to large lightweight cryogenic space optics.

Cryogenic optical measurements of 12-segment-bonded carbon-fiber-reinforced silicon carbide composite mirror with support mechanism.

A 720 mm diameter 12-segment-bonded carbon-fiber-reinforced silicon carbide (C/SiC) composite mirror has been fabricated and tested at cryogenic temperatures. Interferometric measurements show significant cryogenic deformation of the C/SiC composite mirror, which is well reproduced by a model analysis with measured properties of the bonded segments. It is concluded that the deformation is due mostly to variation in coefficients of thermal expansion among segments. In parallel, a 4-degree-of-freedom ball-bearing support mechanism has been developed for cryogenic applications. The C/SiC composite mirror was mounted on an aluminum base plate with the support mechanism and tested again. Cryogenic deformation of the mirror attributed to thermal contraction of the aluminum base plate via the support mechanism is highly reduced by the support, confirming that the newly developed support mechanism is promising for its future application to large-aperture cooled space telescopes.

Microscopic surface structure of C/SiC composite mirrors for space cryogenic telescopes.

We report on the microscopic surface structure of carbon-fiber-reinforced silicon carbide (C/SiC) composite mirrors that have been improved for the Space Infrared Telescope for Cosmology and Astrophysics (SPICA) and other cooled telescopes. The C/SiC composite consists of carbon fiber, silicon carbide, and residual silicon. Specific microscopic structures are found on the surface of the bare C/SiC mirrors after polishing. These structures are considered to be caused by the different hardness of those materials. The roughness obtained for the bare mirrors is 20 nm rms for flat surfaces and 100 nm rms for curved surfaces. It was confirmed that a SiSiC slurry coating is effective in reducing the roughness to 2 nm rms. The scattering properties of the mirrors were measured at room temperature and also at 95 K. No significant change was found in the scattering properties through cooling, which suggests that the microscopic surface structure is stable with changes in temperature down to cryogenic values. The C/SiC mirror with the SiSiC slurry coating is a promising candidate for the SPICA telescope.

Cryogenic optical performance of the ASTRO-F SiC telescope.

The lightweight cryogenic telescope on board the Japanese infrared astronomical satellite, ASTRO-F, which is scheduled to be launched early in 2006, forms an F/6 Ritchey-Chretien system with a primary mirror of 710 mm in diameter. The mirrors of the ASTRO-F telescope are made of sandwich-type silicon carbide (SiC) material, comprising a porous core and a chemical-vapor-deposited coat of SiC on the surface. To estimate the optical performance of the flight model telescope, the telescope assembly was tested at cryogenic temperatures, the total wavefront errors of which were measured by an interferometer from outside a liquid-helium chamber. As a result, the wavefront error obtained at 9 K shows that the imaging performance of the ASTRO-F telescope is diffraction limited at a wavelength of 6.2 microm, which is a little worse than our original goal of diffraction-limited performance at 5.0 microm.