RESUMEN
Human polymorphonuclear leukocytes (PMN) demonstrated the ability to detach Actinomyces viscosus, A. naeslundii and Streptococcus sanguis from saliva-coated hydroxyapatite beads (SHA). Between 60 to 80% of bacteria were detached within 1 hour at PMN-to-bacteria ratios between 1:10 to 1:22. Detachment was enhanced by treating bacteria with fresh but not heat-inactivated normal human serum. Detachment of serum-treated A. viscosus was inhibited by cytochalasin B, L-1-tosylamide-2-phenylethylchloromethyl ketone (TPCK), and deoxyglucose but not colchicine, phenylmethylsulfonyl fluoride (PMSF), N-carbobenzoxy-L-phenylalanine chloromethyl ketone (ZPCK), and sodium azide. In the absence of serum treatment, the detachment of A. viscosus was insensitive to lactose, galactose, and mannose. We conclude that PMN can efficiently detach bacteria from SHA, this detachment is enhanced by serum, and this enhancement is probably dependent upon complement. Additionally, detachment of A. viscosus bound to SHA by PMN (1) does not appear to involve bacterial lectin activity, (2) seems to be dependent upon glycolytic metabolism, microfilament formation, and the activity of a TPCK-sensitive serine protease, and (3) is not sensitive to inhibitors of tubulin polymerization or heme-protein activity.
Asunto(s)
Actinomyces/fisiología , Adhesión Bacteriana , Hidroxiapatitas , Neutrófilos/fisiología , Saliva/fisiología , Streptococcus sanguis/fisiología , Adulto , Adhesión Bacteriana/efectos de los fármacos , Sangre , Citocalasina B/farmacología , Desoxiglucosa/farmacología , Humanos , Cinética , Masculino , Neutrófilos/efectos de los fármacos , Inhibidores de Proteasas/farmacologíaRESUMEN
This study examined the initial attachment of Streptococcus sanguis G9-B and Actinomyces viscosus T14V to saliva-treated powdered enamel and titanium surfaces. Using an in vitro adherence model, significantly lower numbers of Actinomyces viscosus T14V bound to the saliva-treated titanium surface when compared to that of the similarly treated enamel. The binding of Streptococcus sanguis G9-B to titanium or enamel did not vary significantly. A comparison of the percentage of cells bound to the titanium surface revealed that S sanguis cells attached in significantly higher numbers when compared to the A viscosus cells.