Microcontaminants and reproductive impairment of the Forster's tern on Green Bay, Lake Michigan--1983.

For the 1983 nesting season, Forster's tern (Sterna forsteri) reproductive success was significantly impaired on organochlorine contaminated Green Bay, Lake Michigan compared to a relatively uncontaminated inland location at Lake Poygan, Wisconsin. Compared with tern eggs from Lake Poygan, eggs from Green Bay had significantly higher median concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), other polychlorinated dibenzo-p-dioxins (PCDDs), total polychlorinated biphenyls (PCBs), total (three congeners) non-ortho, ortho' PCBs, five individual PCB congeners known to induce aryl hydrocarbon hydroxylase (AHH) and several other organochlorine contaminants. Conversions of analytical concentrations of TCDD and PCB congeners based on relative AHH induction potencies allowed for estimation of total 2,3,7,8-TCDD equivalents. Two PCB congeners, 2,3,3',4,4'- and 3,3',4,4',5-pentachlorobiphenyl (PeCB) accounted for more than 90% of the median estimated TCDD equivalents at both Green Bay and Lake Poygan. The median estimated TCDD equivalents were almost 11-fold higher in tern eggs from Green Bay than in eggs from Lake Poygan (2175 and 201 pg/g). The hatching success of Green Bay sibling eggs from nests where eggs were collected for contaminant analyses was 75% lower at Green Bay than at Lake Poygan. Hatchability of eggs taken from other nests and artificially incubated was about 50% lower for Green Bay than for Lake Poygan. Among hatchlings from laboratory incubation, those from Green Bay weighed approximately 20% less and had a mean liver weight to body weight ratio 26% greater than those from Lake Poygan. In both field and laboratory, mean minimum incubation periods were significantly longer for eggs from Green Bay compared to Lake Poygan (8.25 and 4.58 days, respectively). Mean minimum incubation time for Green Bay eggs in the field was 4.37 days longer than in the laboratory. Hatchability was greatly improved when Green Bay eggs were incubated by Lake Poygan adults in an egg-exchange experiment, but was sharply decreased in Lake Poygan eggs incubated in Green Bay nests. Nest abandonment and egg disappearance were substantial at Green Bay but nil at Lake Poygan. Thus, not only factors intrinsic to the egg, but also extrinsic factors (parental attentiveness), impaired reproductive outcome at Green Bay. The epidemiological evidence from this study strongly suggested that contaminants were a causal factor. AHH-active PCB congeners (intrinsic effects) and PCBs in general (extrinsic effects) appeared to be the only contaminants at the concentrations measured in eggs, capable of producing the effects that were observed at Green Bay.

Contaminant levels in colonial waterbirds from Green Bay and Lake Michigan, 1975-80.

Residues of organochlorine pesticides, polychlorinated biphenyls (PCBs), polybrominated biphenyls (PBBs), polychlorinated styrenes (PCSs), and mercury were measured in the eggs of 10 species of colonial waterbirds nesting in areas around Green Bay or Lake Michigan from 1975 to 1980. Residues also were measured in the carcasses and brains of black-crowned night-herons (Nycticorax nycticorax). The highest residues were of PCBs, DDE, mercury, and dieldrin; for some species, levels of these chemicals possibly were high enough to have caused reproductive effects. Other organochlorine pesticides were found at low levels. Only trace amounts of PCSs and PBBs were found. Eggs of herring gulls (Larus argentatus) collected in 1977 from an island in Lake Michigan contained an average of 100 ppm PCBs and 33 ppm DDE; this was the most-contaminated species. Cattle egrets (Bubulcus ibis), the only bird that is not a fish eater, contained only small quantities of DDE and mercury.