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Vox Sang ; 82(3): 137-40, 2002 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-11952988

RESUMEN

BACKGROUND AND OBJECTIVES: A molecular method for analysing whole-blood samples should be established for quality control of plasma sample logistics. MATERIALS AND METHODS: DNA profiles of retention samples (plasma) were compared to profiles of recent donations (whole blood). DNA extraction, amplification and detection were performed using the Qiagen DNA Blood Mini kit, the AmpFFISTR Profiler Plus Kit and capillary electrophoresis, respectively. RESULTS: Matched pairs of full profiles were obtained for all samples investigated, therefore no deviation from the standardized procedures was detected. CONCLUSIONS: Modified extraction and amplification protocols enabled DNA profiling to be used for the quality control of plasma samples. Hence, DNA profiling can be used in the blood bank as a safe and easy method for quality control of sample logistics.


Asunto(s)
Bancos de Sangre/normas , Donantes de Sangre , Dermatoglifia del ADN , ADN/clasificación , Electroforesis Capilar , Amplificación de Genes , Humanos , Reacción en Cadena de la Polimerasa , Control de Calidad , Sensibilidad y Especificidad , Manejo de Especímenes
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