Utilización de la citomorfología junto con la citometría de flujo en material obtenido por PAAF en lesiones linfoproliferativas. Comparación de coste frente a la biopsia / Use of cytomorphology in combination with flow cytometry in material obtained through FNA in lymphoproliferative lesions. Costs compared with biopsy

Introducción: El uso de la punción-aspiración con aguja fina (PAAF) junto con la citometría de flujo (CMF) constituye una alternativa diagnóstica frente a la biopsia. Objetivos: Se compara la eficacia diagnóstica de la citología más CMF en enfermedades linfoproliferativas y se calcula el coste de la PAAF frente a la biopsia. Resultados: La sensibilidad de la CCF es del 96 por ciento en procesos reactivos y la especificidad del 97 por ciento. En el diagnóstico de los linfomas no Hodgkin, teniendo en cuenta el tipo, la sensibilidad es del 89 por ciento, la especificidad del 100 por ciento y la eficacia del 95 por ciento. La citología unida a la CMF resulta cuatro veces más barata que la biopsia. Conclusiones: Las técnicas de CMF e inmunocitoquímica para aplicar a las PAAF son aconsejables en determinados casos por su rapidez, economía, menor morbilidad y menores molestias para el paciente. Otra perspectiva a considerar es la aplicación de técnicas de biología molecular (AU)

[Interactive morphometry and densitometry for the study of epidermoid carcinoma of the larynx. Preliminary results]. / Aplicación de la morfometría y densitometría interactivas al estudio del carcinoma epidermoide laríngeo. Resultado preliminar.

At present the utilization of quantitative techniques in anatomopathological studies is becoming more and more frequent, so adding the possibility of gaining measures and numerical assessments in front of conventional quantitative evaluations. In the paper the AA. report their first attempt with conventional slides of epidermoid carcinomas of the larynx, taken from the files. The aim has been to check out the contribution of this procedure compared with other traditional pathological techniques.

[Prognostic value of the degree of differentiation and the peri-tumor lymphoid infiltration in epidermoid carcinoma of the larynx]. / Valor pronóstico del grado de diferenciación y el infiltrado linfoide peritumoral en el carcinoma epidermoide de laringe.

By multiple linear regression the authors show a closed relationship between survival and conventional anatomopathologic data as grade of differentiation and of lymphoid infiltration of the primary tumor. This retrospective pathological study showed that various degrees of squamous cell differentiation may be recognized for malignant laryngeal lesions and that such distinction may be of prognostic significance.

[Vascular microinvasion in laryngeal epidermoid carcinoma]. / Microinvasión vascular en el carcinoma epidermoide laríngeo.

Microvascular invasion by carcinoma cells is one of the first steps for the potential development of metástasis. The presence of squamous cell carcinoma within capillaries and/or venules in the immediate vicinity of primary lesions of the oral cavity and oropharynx may be related to regional lymph node metastasis. To evaluate this possibility in laryngeal carcinomas we have reviewed the histopathologic features of 48 cases of this type of tumor with simultaneous surgical treatment of the primary neoplasms and the neck. In our opinion the histologic finding of such invasion is not pathognomonic for establishment of regional metástasis in epidermoid carcinoma of the larynx.

[The importance of laryngeal compartmentation in the limitation of tumor extension]. / Importancia de la compartimentación laríngea en la limitación de la extensión tumoral.

Histologic specimens from 75 patients with squamous cell carcinoma of the larynx treated exclusively by surgery were studied with special attention paid to the growth of the tumor in relation with the different laryngeal compartments. Some aspects traditionally admitted are discussed. The result show a not very close relation between the classic anatomic descriptions and the tumoral growing.

[Prognostic value of lymph node histologic pattern in epidermoid carcinoma of the larynx]. / Interés pronóstico del patrón histológico ganglionar en el carcinoma epidermoide de laringe.

Histologic specimens from 48 patients with squamous cell carcinoma of the larynx treated exclusively by surgery were studied with special attention paid to the reactivity of regional lymph nodes. The lymph nodes were classified into four microscopic patterns of immune response: lymphocytic predominance, sinus histiocytosis, germinal center predominance and unstimulated or lymphocytic depletion. As a result no correlation were then made between the patterns and survival rate at 5 year follow-up.

[A morpho-photometric study of laryngeal epidermoid carcinoma]. / Estudio morfofotométrico del carcinoma epidermoide de laringe.

At the moment the use of quantitative techniques in anatomopathology is more and more frequent. In this study the authors use for the first time the morpho-photometry approach in epidermoid carcinomas of the larynx coming from filed material. In this way they are checking if this method adds anything to conventional anatomopathology.

[A comparative study of the prognostic value of morphometric data and of conventional pathological anatomy in laryngeal epidermoid carcinoma]. / Estudio comparativo del valor pronóstico de los datos morfofotométricos y de la anatomía patológica convencional en el carcinoma epidermoide de laringe.

At the moment the use of quantitative techniques is not very frequent in standard anatomopathologic studies of epidermoid carcinoma of the larynx. By "multiple linear regression" the authors show a closed relationship between survival and conventional anatomopathologic data as grade of differentiation and of lymphoid infiltration of the primary tumor.

[Study of histological preparations of laryngeal epidermoid carcinoma using image analysis]. / Estudio de preparaciones histológicas de carcinoma epidermoide laríngeo mediante análisis de imagen.

At the present application of quantitative techniques in anatomopathologic studies is becoming more and more frequent, adding the possibility of numeric and quantitative evaluation. In this study the authors make a first attempt with one of these methods, the image analysis using conventional slides of epidermoid carcinomas from larynx.

[Prognostic value of the grade of malignancy in epidermoid carcinoma of the larynx]. / Valor pronóstico del grado de malignidad en el carcinoma epidermoide de la laringe.

At the moment the use of quantitative techniques in anatomopathology are becoming more and more frequent. In this study, with the mathematical transformation of morphometric data obtained from cell populations of epidermoid carcinoma of the larynx, the authors arrive at the "grade of malignancy" for every patient which has in itself prognostic value without taking into account the other anatomopathologic or clinical parameters.

[Use of morphophotometry in the study of the kinetics of cell populations in laryngeal epidermoid carcinoma]. / Aplicación de la morfofotometría al estudio de la cinética de poblaciones celulares en el carcinoma epidermoide laríngeo.

The use of morpho-photometric techniques in anatomopathology is, on the whole, not very frequent at the moment. In this study the authors show the possible applications of these quantitative methods for the evaluation of kinetics characteristics of cell population in epidermoid carcinomas of the larynx.

[Bone marrow biopsy in myeloproliferative syndromes: prognostic value of cellular quantification]. / Biopsia medular en los síndromes mieloproliferativos: valor pronóstico de la cuantificación celular.

The proportion of granulocytes, erythroblasts, immature cells and megakaryocytes was evaluated when revising the bone-marrow biopsy specimens of 36 patients with myeloproliferative syndromes. These proportions were compared between themselves, a so-called Z value being thus attained; such value resulted from summing the proportion of granulocytes, megakaryocytes and immature cells and subtracting the proportion of erythroblasts. According to its value, above or below the mean, the Z figure correlates with the survival, the difference between both groups appearing as statistically significant (p = 0.02).