RESUMEN
A universal mathematical approach (algorithm) to assessment of the efficacy of new preparations for the diagnosis of rotavirus infection, analysis and processing of the results involves assessment of the detection rate of infection, specificity of preparations, probability of positive and negative diagnoses, total index of coincidence of results of diagnosis with the reference method, and chi 2 and phi2 coefficients indicating the reliability of relation to the reference method and the strength of this relation. Diatest software is proposed for accelerating statistical processing of the results. Use of the algorithm and software permits assessing the efficacy of diagnostic agents for rotavirus and other infections.
Asunto(s)
Técnicas para Inmunoenzimas , Infecciones por Rotavirus/diagnóstico , Algoritmos , Distribución de Chi-Cuadrado , Humanos , Sensibilidad y Especificidad , Programas InformáticosRESUMEN
The diagnostic value of the first experimental production batches of assay kit "DIAGN-A-HEP", produced at the Institute of Poliomyelitis and Viral Encephalitides (USSR Acad. Med. Sci.) and intended for the determination of IgM to hepatitis A virus (HAV) in the enzyme immunoassay (EIA), has been studied in comparison with that of the internationally known and widely approved commercial EIA system "HAVAB-MEIA" for the determination of antibodies to HAV (the product of Abbott, USA). The study has revealed that the EIA kit "DIAGN-A-HEP" is highly sensitive and specific, and the diagnostic value of this kit is not inferior to that of the commercial assay system "HAVAB-MEIA". On the basis of this study the use of the EIA kits "DIAGN-A-HEP" in medical practice has been allowed by the decree of the Ministry of Health of the USSR.