Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Más filtros










Base de datos
Intervalo de año de publicación
1.
Elife ; 102021 09 29.
Artículo en Inglés | MEDLINE | ID: mdl-34586065

RESUMEN

Sensory neurons with cell bodies in dorsal root ganglia (DRG) represent a useful model to study axon regeneration. Whereas regeneration and functional recovery occurs after peripheral nerve injury, spinal cord injury or dorsal root injury is not followed by regenerative outcomes. Regeneration of sensory axons in peripheral nerves is not entirely cell autonomous. Whether the DRG microenvironment influences the different regenerative capacities after injury to peripheral or central axons remains largely unknown. To answer this question, we performed a single-cell transcriptional profiling of mouse DRG in response to peripheral (sciatic nerve crush) and central axon injuries (dorsal root crush and spinal cord injury). Each cell type responded differently to the three types of injuries. All injuries increased the proportion of a cell type that shares features of both immune cells and glial cells. A distinct subset of satellite glial cells (SGC) appeared specifically in response to peripheral nerve injury. Activation of the PPARα signaling pathway in SGC, which promotes axon regeneration after peripheral nerve injury, failed to occur after central axon injuries. Treatment with the FDA-approved PPARα agonist fenofibrate increased axon regeneration after dorsal root injury. This study provides a map of the distinct DRG microenvironment responses to peripheral and central injuries at the single-cell level and highlights that manipulating non-neuronal cells could lead to avenues to promote functional recovery after CNS injuries or disease.


Asunto(s)
Ganglios Espinales/citología , Células Receptoras Sensoriales/fisiología , Animales , Axones , Biomarcadores/metabolismo , Proliferación Celular , Microambiente Celular , Fenofibrato/administración & dosificación , Ganglios Espinales/metabolismo , Macrófagos/citología , Ratones , PPAR alfa/metabolismo , Células Receptoras Sensoriales/citología , Células Receptoras Sensoriales/metabolismo , Análisis de la Célula Individual , Traumatismos de la Médula Espinal/patología , Traumatismos de la Médula Espinal/fisiopatología
2.
Neuron ; 88(4): 720-34, 2015 Nov 18.
Artículo en Inglés | MEDLINE | ID: mdl-26526390

RESUMEN

Injured peripheral neurons successfully activate a proregenerative transcriptional program to enable axon regeneration and functional recovery. How transcriptional regulators coordinate the expression of such program remains unclear. Here we show that hypoxia-inducible factor 1α (HIF-1α) controls multiple injury-induced genes in sensory neurons and contribute to the preconditioning lesion effect. Knockdown of HIF-1α in vitro or conditional knock out in vivo impairs sensory axon regeneration. The HIF-1α target gene Vascular Endothelial Growth Factor A (VEGFA) is expressed in injured neurons and contributes to stimulate axon regeneration. Induction of HIF-1α using hypoxia enhances axon regeneration in vitro and in vivo in sensory neurons. Hypoxia also stimulates motor neuron regeneration and accelerates neuromuscular junction re-innervation. This study demonstrates that HIF-1α represents a critical transcriptional regulator in regenerating neurons and suggests hypoxia as a tool to stimulate axon regeneration.


Asunto(s)
Axones/metabolismo , Ganglios Espinales/citología , Regulación de la Expresión Génica , Subunidad alfa del Factor 1 Inducible por Hipoxia/genética , Hipoxia/genética , Neuronas Motoras/metabolismo , Regeneración Nerviosa/genética , Traumatismos de los Nervios Periféricos/genética , Células Receptoras Sensoriales/metabolismo , Animales , Células Cultivadas , Ganglios Espinales/metabolismo , Técnicas de Silenciamiento del Gen , Hipoxia/metabolismo , Técnicas In Vitro , Ratones , Unión Neuromuscular , Traumatismos de los Nervios Periféricos/metabolismo , Nervio Ciático/lesiones , Nervio Ciático/metabolismo , Factor A de Crecimiento Endotelial Vascular/metabolismo
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...