[Experimental studies to culture bacteria from blood specimen with an addition of ampicillin in various nutrient media (author's transl)]. / Experimentelle Untersuchungen zur Anzüchtung von Bakterien aus Blutproben unter Ampicillinzugabe in verschiedenen Nährmedien.

Ampicillin-sensitive or -resistant Staphylococcus aureus and Klebsiella strains were cultured in various nutrient media as prototypes of the agents of sepsis isolated in bacteriological routine analysis. In each case, 2 ml of defibrinated human blood containing 100 and 1000 cells, 8 gamma and 80 gamma ampicillin/1 ml blood respectively were added to 50 ml of nutrient medium. The following media were used. 1. casein soya-bean meal peptone broth (Oxoid), 2. tryptose-phosphate medium (Oxoid), 3. dextrose broth (Oxoid), 4. brain-heart-dextrose medium (Oxoid), 5. brain-heart infusion, autoclaved (Difco), 6. brain-heart infusion, after sterile filtration (Difco), 7. vacutainer culture bottles (BD) prepared medium, 8. micrognost blood culture bottles (Biotest) prepared medium. While the sensitive staphylococcus strain exhibited a slower growth than the sensitive Klebsiella strain in all nutrient media, the growth rate of the two resistant variants was approximately the same for an initial count of 100 cells per ml of blood. Among the resistant staphylococci the higher initial count of the inoculum resulted in an improved growth. After addition of 8 gamma or 80 gamma ampicillin/1 ml blood the sensitive staphylococcus strain did not show any grwoth irrespective of the inoculated number of cells while the sensitive Klebsiella strain multiplied irrespective of the initial number of cells. After 24 hours the resistant staphylococci and Klebsiella strains of which 1000 cells each had been used for inoculation exhibited growth in almost all media used.

[Useful information on exchange and humidification of air in rooms (author's transl)]. / Wissenswertes über Raumluft-Wechsel und Raumluft-Befeuchtung

The physical and also to some extent physiological facts which underlie the quality of the air we breathe-primarily exchange and humidification of the air in rooms - are presented to make clear what hygienic significance is attributable to these climatic factors. It is essentially a matter of the air in densely populated rooms, particularly under the complicating conditions of the cold and cool seasons. The ways which can be adopted to make the indoor climate tolerable by economic and technically simple means are shown. For this, the co-operation of all offices of the civil service is absolutely necessary to promote the quality of this vital condition of the air in rooms.

[Bactericidal activity of serum and chemotherapy in sensitive and resistant exciter (author's transl)]. / Blutbakterizidie und Chemotherapie bei empfindlichen und resistenten Erregern

Comparing examinations with Ampicillin sensitive and resistant bacteria-strains show that the bactericidal activity of serum is dependent on the bacteria-strains, on the Ampicillin sensitivity of the particular exciter and on the number of bacteria/ml (germ count). Bactericide effect could always be obtained with sensitive strains as a result of additional chemotherapy. With several resistant strains a bactericide effect could not be obtained in this case the continuous optimal Ampicillin addition was the decisive factor. Because of the extremely complicated process of the bactericide one should not make general conclusions from the individual experimental results.

[A device for safe handling of vacuum-dried microbes (author's transl)]. / Eine Einrichtung zum gefahrlosen Umgang mit vacuumgetrockneten Mikroben

By analogous application of the way of packing which is common for the transportation of infectious material (glass tubes in protective covers) in the handling of vaccuum-dried microbes (ampoule within the ampoule) the risk encountered when opening such receptacles may be reduced to an almost complete absence. Technical details are explained by a schematic representation. The method is suitable for the preservation of freeze-dried vaccines. All processes involved in the practical performance of the method which is ready for routine application are considered in detailed instructions for use.

[Optimal preservation of function in vacuum-dried bacteria (author's transl)]. / Ein Verfahren zur optimalen Funktionserhaltung vacuumgetrockneter Bakterien

Experience has shown that there are natural processes assuring a good preservation of virulence of delicate bacteria over intervals between epidemic outbreaks. Similarly, fetal bovine serum enriched with 10% saccharose was found to be an ideal medium for rendering bacteria well preservable without losing essential functional characteristics by means of vacuum-drying. Cotton wool DAB 7 has proved to be a good support. The available findings would let also delicate aerobic and anaerobic bacteria appear to be suitable for this kind of preservation, retaining their full capacity to multiply for at least one year inclusive of sensitive functional parameters. Results have been grouped in a number of synoptic tables, Bacteria that would not admit of the method of preservation described, have not been found thus far.