RESUMEN
To study the effect of ischemia preconditioning (IP) on renal ischemia/reperfusion (I/R)-associated functional injury and expression of renal adhesion molecules in rats. The ischemia preconditioning plan adopted in this experiment involved renal warm ischemia for 6 min. and blood flow for 4 min., repeated four times. The Wistar rat kidneys used for warm ischemia preconditioning were subjected to 60 min of renal warm ischemia followed by reperfusion. The rat kidneys with ischemia/reperfusion were compared with the ischemia preconditioning group to observe rat renal function and changes in the expression of renal adhesion molecules ICAM-1, P--Selectin, and E-Selectin. The expression of rat renal adhesion molecules (ICAM-1, P-Selectin, and E-Selectin) with ischemia preconditioning was significantly lower than that of the ischemia/reperfusion group. Serum creatinine was significantly lower than that in the ischemia/reperfusion group after 48 hours. Ischemia preconditioning has a protective effect on renal function. Reduced expression of renal adhesion molecules is likely a mechanism involved in the observed protection.
Asunto(s)
Animales , Femenino , Masculino , Ratas , Precondicionamiento Isquémico/métodos , Riñón/irrigación sanguínea , Daño por Reperfusión/prevención & control , Moléculas de Adhesión Celular/análisis , Creatinina/sangre , Modelos Animales de Enfermedad , Selectina E/análisis , Inmunohistoquímica , Riñón/patología , Selectina-P/análisis , Ratas Wistar , Daño por Reperfusión/patología , Factores de TiempoRESUMEN
OBJECTIVE: To study the effect of ischemia preconditioning (IP) on renal ischemia/reperfusion (I/R)-associated functional injury and expression of renal adhesion molecules in rats. MATERIALS AND METHODS: The ischemia preconditioning plan adopted in this experiment involved renal warm ischemia for 6 min. and blood flow for 4 min., repeated four times. The Wistar rat kidneys used for warm ischemia preconditioning were subjected to 60 min of renal warm ischemia followed by reperfusion. The rat kidneys with ischemia/reperfusion were compared with the ischemia preconditioning group to observe rat renal function and changes in the expression of renal adhesion molecules ICAM-1, P-Selectin, and E-Selectin. RESULTS: The expression of rat renal adhesion molecules (ICAM-1, P-Selectin, and E-Selectin) with ischemia preconditioning was significantly lower than that of the ischemia/reperfusion group. Serum creatinine was significantly lower than that in the ischemia/reperfusion group after 48 hours. CONCLUSIONS: Ischemia preconditioning has a protective effect on renal function. Reduced expression of renal adhesion molecules is likely a mechanism involved in the observed protection.
Asunto(s)
Precondicionamiento Isquémico/métodos , Riñón/irrigación sanguínea , Daño por Reperfusión/prevención & control , Animales , Moléculas de Adhesión Celular/análisis , Creatinina/sangre , Modelos Animales de Enfermedad , Selectina E/análisis , Femenino , Inmunohistoquímica , Riñón/patología , Masculino , Selectina-P/análisis , Ratas , Ratas Wistar , Daño por Reperfusión/patología , Factores de TiempoRESUMEN
OBJECTIVE: To investigate the effects of the second renal transplantation on sexual function. METHODS: Thirty kidney graft recipients, including 29 cases of the second renal transplantation and 1 case of simultaneous dual kidney transplantation, responded to the questionnaire. The penis cavernosal artery flow of these patients were examined by color doppler ultrasonography. Of the 30 recipients, 9 underwent bilateral kidney transplantation with their bilateral external iliac arteries anastomosed to the donors' renal arteries (Group A), 10 recipients with their unilateral external iliac arteries and the other internal iliac arteries anastomosed to the donors' renal arteries (Group B), the other 10 with their internal iliac arteries anastomosed to the donors' renal arteries (Group C). RESULTS: Eight recipients of Group A, 7 of Group B, and 5 of Group C were restored to normal sexual function 6 months after kidney transplantation. The peak systole velocity (PSV) in Group C was slower than in Groups A and B. CONCLUSION: Kidney transplantation with the second internal iliac arteries anastomosed to donors' renal arteries may affect the sexual function of the recipients, but some might enjoy satisfactory sexual life some time after the establishment of lateral branch circulation.