RESUMEN
Aim: To evaluate the histologic and histomorphometric effects of hyaluronic acid (HA) gel with or without acellular dermal matrix allograft (ADMA) on periodontal regeneration in Class II furcation defects in dogs. Materials and methods: Class II furcation defects were surgically created in the mandibular first and second premolars bilaterally in eight beagle dogs. The Class II furcation defects were assigned randomly, using the split-mouth design, into the test and control sides. The teeth on the test sides were equally and randomly divided into the HA/ADMA group (n = 8) treated with 0.8% HA gel followed by ADMA, and the HA-only group (n = 8) treated with 0.8% HA only. The furcation defects of the control sides (n = 16) were subjected to open flap debridement (OFD group). The animals were euthanized for histologic and histomorphometric analyses after one month (n = 4) and three months (n = 4). Results: At one month, the newly formed bone area (NFBA) was larger in the HA/ADMA (6.23 ± 1.41 mm2) and HA-only (5.90 ± 1.43 mm2) groups than in the OFD group (2.42 ± 1.62 mm2) (p < 0.05). The newly formed cementum (NFAC) and periodontal ligament (NFPL) were similar in the HA/ADMA and HA-only groups but significantly lesser in the OFD group (p < 0.05.) At three months, the NFBA, NFAC, and NFPL were greater in the HA/ADMA group than in the HA-only group (p < 0.05). New regenerative tissue was significantly greater in both the test groups than in the OFD group (p < 0.05), while epithelial downgrowth predominated the healing in the latter. Conclusions: These results suggest that HA with ADMA positively affects the periodontal regeneration and wound healing in Class II furcation defects.
RESUMEN
OBJECTIVE: The present work evaluated histologically and immunohistochemically the expression of leptin during healing of the incisional oral mucosal wound in diabetic rats as compared to healthy rats. METHODS: Twenty-four adult male Sprague-Dawley rats weighing on average 150-200 g were allocated equally into two groups: Group I (control) and Group II (diabetic). Diabetes was induced by a single intraperitoneal injection of streptozotocin dissolved in distilled water. Each animal received experimental incision in buccal mucosa and sutured, and the specimens were collected from the buccal mucosa of each animal at intervals of 7, 14, and 21 days and routinely processed for H and E and immunohistochemical staining for leptin. All measurement data were calculated as a mean ± standard deviation. RESULTS: Leptin expression was observed in the epithelium and the vascular endothelial cells in both groups. In both the control and diabetic groups, the expression of leptin was significantly increased with time, and there was an extreme highly significant increase in the control group than in diabetic group after 7, 14, and 21 days (P = 0.000). CONCLUSION: The results of the present study suggested that leptin may promote wound healing in rat's normal oral mucosa more than in diabetic. Further studies are needed to clarify the exact molecular mechanisms of leptin's effects on wound healing and to determine the usefulness of leptin as a treatment to promote wound healing in the oral mucosa in diabetic and non-diabetic patients.