RESUMEN
Analysis of factors that lead to the functionality of transcriptional activation domains remains a crucial and yet challenging task owing to the significant diversity in their sequences and their intrinsically disordered nature. Almost all existing methods that have aimed to predict activation domains have involved traditional machine learning approaches, such as logistic regression, that are unable to capture complex patterns in data or plain convolutional neural networks and have been limited in exploration of structural features. However, there is a tremendous potential in the inspection of the structural properties of activation domains, and an opportunity to investigate complex relationships between features of residues in the sequence. To address these, we have utilized the power of graph neural networks which can represent structural data in the form of nodes and edges, allowing nodes to exchange information among themselves. We have experimented with two kinds of graph formulations, one involving residues as nodes and the other assigning atoms to be the nodes. A logistic regression model was also developed to analyze feature importance. For all the models, several feature combinations were experimented with. The residue-level GNN model with amino acid type, residue position, acidic/basic/aromatic property and secondary structure feature combination gave the best performing model with accuracy, F1 score and AUROC of 97.9%, 71% and 97.1% respectively which outperformed other existing methods in the literature when applied on the dataset we used. Among the other structure-based features that were analyzed, the amphipathic property of helices also proved to be an important feature for classification. Logistic regression results showed that the most dominant feature that makes a sequence functional is the frequency of different types of amino acids in the sequence. Our results consistent have shown that functional sequences have more acidic and aromatic residues whereas basic residues are seen more in non-functional sequences.
RESUMEN
Lung cancer is a leading cause of death throughout the world. Because the prompt diagnosis of tumors allows oncologists to discern their nature, type, and mode of treatment, tumor detection and segmentation from CT scan images is a crucial field of study. This paper investigates lung tumor segmentation via a two-dimensional Discrete Wavelet Transform (DWT) on the LOTUS dataset (31,247 training, and 4458 testing samples) and a Deeply Supervised MultiResUNet model. Coupling the DWT, which is used to achieve a more meticulous textural analysis while integrating information from neighboring CT slices, with the deep supervision of the model architecture results in an improved dice coefficient of 0.8472. A key characteristic of our approach is its avoidance of 3D kernels (despite being used for a 3D segmentation task), thereby making it quite lightweight.
Asunto(s)
Procesamiento de Imagen Asistido por Computador , Neoplasias Pulmonares , Humanos , Procesamiento de Imagen Asistido por Computador/métodos , Neoplasias Pulmonares/diagnóstico por imagen , Tomografía Computarizada por Rayos X , Análisis de OndículasRESUMEN
The coronavirus disease 2019 (COVID-19) has resulted in an ongoing pandemic worldwide. Countries have adopted non-pharmaceutical interventions (NPI) to slow down the spread. This study proposes an agent-based model that simulates the spread of COVID-19 among the inhabitants of a city. The agent-based model can be accommodated for any location by integrating parameters specific to the city. The simulation gives the number of total COVID-19 cases. Considering each person as an agent susceptible to COVID-19, the model causes infected individuals to transmit the disease via various actions performed every hour. The model is validated by comparing the simulation to the real data of Ford County, KS, USA. Different interventions, including contact tracing, are applied on a scaled-down version of New York City, USA, and the parameters that lead to a controlled epidemic are determined. Our experiments suggest that contact tracing via smartphones with more than 60% of the population owning a smartphone combined with city-wide lockdown results in the effective reproduction number (R t ) to fall below 1 within 3 weeks of intervention. For 75% or more smartphone users, new infections are eliminated, and the spread is contained within 3 months of intervention. Contact tracing accompanied with early lockdown can suppress the epidemic growth of COVID-19 completely with sufficient smartphone owners. In places where it is difficult to ensure a high percentage of smartphone ownership, tracing only emergency service providers during a lockdown can go a long way to contain the spread. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at (10.1007/s12559-020-09801-w).