RESUMEN
The phase diagram of the Langmuir film of diacetylene alcohol-henicosa-5,7-diyn-1-ol-is investigated by means of surface pressure versus surface area isotherms, Brewster angle microscopy, X-ray reflectivity, and grazing incident X-ray diffraction. Among the usual phases described in the generic phase diagram of small head group molecules, one observes an unexpected reversible transition from an ordered condensed phase to a disordered one upon increasing the surface pressure. We postulate that the origin of this unusual, unprecedented transition results from the competition between the interactions between the diacetylene blocks in the hydrophobic chain and the hydrogen bonds between head groups and water.
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In this work, Langmuir films of two highly fluorinated fatty alcohols, CF3(CF2)12CH2OH (F14OH) and CF3(CF2)16CH2OH (F18OH), were studied. Atomic Force Microscopy (AFM) images of the films transferred at zero surface pressure and low surface density onto the surface of silicon wafers by the Langmuir-Blodgett technique revealed, for the first time, the existence of solid-like domains with well-defined mostly hexagonal (starry) shapes in the case of F18OH, and with an entangled structure of threads in the case of F14OH. A (20:80) molar mixture of the two alcohols displayed a surprising combination of the two patterns: hexagonal domains surrounded by zigzagging threads, clearly demonstrating that the two alcohols segregate during the 2D crystallization process. Grazing Incidence X-Ray Diffraction (GIXD) measurements confirmed that the molecules of both alcohols organize in 2D hexagonal lattices. Atomistic Molecular Dynamics (MD) simulations provide a visualization of the structure of the domains and allow a molecular-level interpretation of the experimental observations. The simulation results clearly showed that perfluorinated alcohols have an intrinsic tendency to aggregate, even at very low surface density. The formed domains are highly organized compared to those of hydrogenated alcohols with similar chain length. Very probably, this tendency is a consequence of the characteristic stiffness of the perfluorinated chains. The diffraction spectrum calculated from the simulation trajectories compares favorably with the experimental spectra, fully validating the simulations and the proposed interpretation. The present results highlight for the first time an inherent tendency of perfluorinated chains to aggregate, even at very low surface density, forming highly organized 2D structures. We believe these findings are important to fully understand related phenomena, such as the formation of hemi-micelles of semifluorinated alkanes at the surface of water and the 2D segregation in mixed Langmuir films of hydrogenated and fluorinated fatty acids.
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Due to the characteristic chain rigidity and weak intermolecular interactions of perfluorinated substances, the phase diagram of Langmuir monolayer formed by perfluorinated molecules has been interpreted so far as displaying only two phases, a 2D gas (G) and a liquid condensed (LC). However, in this work, we presented Grazing Incidence X-ray Diffraction measurements, which exhibit two diffraction peaks on the transition plateau: One is the signature of the hexagonal structure of the LC phase, the second one is associated to the low-density fluid phase and is thus more ordered than expected for a 2D gas or a typical fluid phase. Atomistic molecular dynamics simulations, performed on the transition plateau, revealed the existence of clusters in which domains of vertical molecules organized in a hexagonal lattice coexist with domains of parallel lines formed by tilted molecules, a new structure that could be described as a "2D smectic C" phase. Moreover, the diffraction spectrum calculated from the simulation trajectories compared favorably with the experimental spectra, fully validating the simulations and the proposed interpretation. The results were also in agreement with the thermodynamic analysis of the fluid phase and X-ray Reflectivity experiments performed before and after the transition between these two phases.
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Ácidos Grasos/química , Tensoactivos/química , Adsorción , Halogenación , Simulación de Dinámica Molecular , Transición de Fase , Termodinámica , Difracción de Rayos XRESUMEN
We studied by means of Grazing Incidence X-ray Diffraction (GIXD) coupled with X-ray fluorescence spectroscopy the structure of a behenic acid monolayer spread at the surface of Mg2+/Mn2+ mixed aqueous solutions. For the pure Mg2+ and Mn2+ aqueous solutions, the cations induce at the surface different 2D lattice superstructures of the organic monolayer. These superstructures correspond to an inorganic organized monolayer anchored to the hydrophilic group of the ordered behenic acid monolayer. Among the various diffraction peaks, we focused on those characteristics of the behenic acid oblique cell. As the Mg2+ mole fraction x increases in the Mg2+/Mn2+ mixed subphase, a continuous evolution of the oblique cell parameters is observed indicating the insertion of Mg2+ cations in the Mn2+ ordered monolayer. Then, a further increase leads to the appearance of a coexistence between two oblique surface phases. The cell parameters of both phases evolve continuously along the x range of the transition until a single Mg-rich ordered phase is detected. However, although the intensities of the peaks in the coexistence region are in agreement with a first-order phase transition, the cell parameters evolve simultaneously. Considering a thermodynamics analysis, this evidences that, apart from the concentration, another unidentified intensive parameter is varying. We suggest that it is the ionic strength, which appears to be strongly related to the concentrations.
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Semifluorinated alkanes are known to form peculiar nano-structured monolayers on the surface of water. A comprehensive analysis of in situ Grazing Incidence Small Angle X-ray Scattering (GISAXS) proves that the structure of their condensed phase consists of domains of upright molecules surrounded by lying down molecules. Such a model explains the non-coalescence of the domains and is in agreement with the high resolution AFM images of monolayers at the surfaces of solid substrates. The interaction of the lying molecule dipoles with the dipole of the water surface is proposed to explain the observed structuration.
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We describe the surface behavior of PS-b-PAA monolayers at the air/water interface using N,N-dimethyformamide (DMF) as spreading solvent. At low pH, when the PAA blocks are neutral, the surface pressure versus molecular area isotherm shows a pseudoplateau associated with the presence of remaining spreading solvent molecules in the monolayer, as we described in a former study (Guennouni et al., Langmuir, 2016). We show here that the width of the plateau decreases when increasing pH up to its complete disappearance at high pH, when PAA blocks are fully charged, although two regimes of compressibilities on the isotherm still exist. A refined structural study at pH 9 combining specular neutron reflectivity (SNR), grazing-incidence small-angle X-ray scattering (GISAXS), and atomic force microscopy (AFM) in liquid measurements shows that (i) PAA blocks are stretched in solution, as expected from polyelectrolyte brushes in the osmotic regime; (ii) the system undergoes a spinodal decomposition during deposit at the air/water interface in the presence of DMF. Upon compression, the Qxy* position of the peak associated with the spinodal structure remains almost constant but its intensity evolves strongly and passes through a maximum at intermediate pressures. This reveals two operating processes in the system: strong electrostatic repulsions between chains that prevent in-plane reorganizations and force such reorganizations to occur from the surface to the volume and progressive expulsion of the DMF molecules from the monolayer. These processes have antagonist effects on the intensity of the peak: the increase of the repulsions makes it more pronounced, whereas the expulsion of solvent makes it vanish due to the loss of contrast.
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We present an in situ structural study of the surface behavior of PS-b-PAA monolayers at the air/water interface at pH 2, for which the PAA blocks are neutral and using N,N-dimethyformamide (DMF) as spreading solvent. The surface pressure versus molecular area isotherm shows a perfectly reversible pseudoplateau over several cycles of compression/decompression. The width of such plateau enlarges when increasing temperature, conversely to what is classically observed in the case of an in-plane first order transition. We combined specular neutron reflectivity (SNR) experiments with contrast variation to solve the profile of each block perpendicular to the surface with grazing-incidence small-angle scattering (GISAXS) measurements to determine the in-plane structure of the layer. SNR experiments showed that both PS and PAA blocks remain adsorbed on the surface for all surface pressure probed. A correlation peak at Q(xy)* = 0.021 Å(-1) is evidenced by GISAXS at very low surface pressure which intensity first increases on the plateau. When compressing further, its intensity decays while Q(xy)* is shifted toward low Q(xy). The peak fully disappears at the end of the plateau. These results are interpreted by the formation of surface aggregates induced by DMF molecules at the surface. These DMF molecules remain adsorbed within the PS core of the aggregates. Upon compression, they are progressively expelled from the monolayer, which gives rise to the pseudoplateau on the isotherm. The intensity of the GISAXS correlation peak is set by the amount of DMF within the monolayer as it vanishes when all DMF molecules are expelled. This result emphizes the role of the solvent in Langmuir monolayer formed by amphiphilic copolymers which hydrophobic and hydrophilic parts are composed by long polymer chains.
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In order to form a nanostructured metallic layer below a Langmuir monolayer, radiolysis synthesis was carried out in an adapted geometry that we call surface X-ray radiolysis. In this procedure, an X-ray beam produced by a synchrotron beamline intercepts the surface of an aqueous metal-ion solution covered by a Langmuir monolayer at an angle of incidence below the critical angle for total internal reflection. Underneath the organic layer, the X-ray beam induces the radiolytic synthesis of a nanostructured metal-organic layer whose ultrathin thickness is defined by the vertical X-ray penetration depth. We have shown that increasing the X-ray flux on the surface, which considerably enhances the kinetics of the silver layer formation, results in a second growth regime of silver nanocrystals. Here the formation of the oriented thin layer is followed by the appearance of a 3D powder of silver clusters.
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Multilayer films of semifluorinated alkanes (SFAs) at the air/water interface were studied in situ by grazing incidence small-angle X-ray scattering (GISAXS). The results provide evidence that the first layer in contact with the water subphase, buried below the overlayers, exhibits the same supramolecular hexagonal structure that is observed in the monolayer before the collapse, at non-zero surface pressure. We believe this result clearly demonstrates the major role of the interactions between the first layer of SFAs and the water subphase to the formation of the structure.
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The adsorption of zinc cations under behenic acid Langmuir monolayers was investigated by means of isotherm measurements, grazing incidence X-ray diffraction and Brewster angle microscopy. The structure of the films was characterized as a function of Zn(2+) concentration, for three different counterions (chloride, iodide, bromide) and at two subphase pHs (5.5 and 7.5). At pH 5.5 and in the studied concentration range, Zn(2+) adsorption leads to a condensation of the fatty acid monolayer with the same phase transitions as over pure water. In contrast, at higher pH the organic X-phase is evidenced immediately above a concentration threshold without any ion organization. Even though Cu(2+) and Zn(2+)cations induce both the fatty acid X-phase, the kinetics of its formation appears strongly different. Indeed, as for Mg(2+) and Cd(2+), the intermediate new I-structure is evidenced in the course of Zn(2+) adsorption although superstructures are observed only for Mg(2+) and Cd(2+). However, for Zn(2+), the I-phase evolves to the final state through a new structure called X' and a continuous X'-X transition. Finally, any effect of the counterion is evidenced neither during the kinetic process nor in the final state.
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Ácidos Grasos/química , Zinc/química , Cationes Bivalentes , Concentración de Iones de Hidrógeno , Cinética , Estructura Molecular , Soluciones , Difracción de Rayos XRESUMEN
We have determined the structure formed at the air-water interface by semifluorinated alkanes (C(8)F(17)C(m)H(2m+1) diblocks, F8Hm for short) for different lengths of the molecule (m = 14, 16, 18, 20) by using surface pressure versus area per molecule isotherms, Brewster angle microscopy (BAM), and grazing incidence x-ray experiments (GISAXS and GIXD). The behavior of the monolayers of diblocks under compression is mainly characterized by a phase transition from a low-density phase to a condensed phase. The nonzero surface pressure phase is crystalline and exhibits two hexagonal lattices at two different scales: a long-range-order lattice of a few tens of nanometers lateral parameter and a molecular array of about 0.6 nm parameter. The extent of this organization is sufficiently large to impact larger scale behavior. Analysis of the various compressibilities evidences the presence of non organized molecules in the monolayer for all 2D pressures. At room temperature, the self-assembled structure appears generic for all the F8Hm investigated.
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Alcanos/química , Flúor/química , Nanotecnología , Microscopía de Fuerza Atómica , Dispersión de Radiación , Propiedades de Superficie , Tensoactivos/química , Difracción de Rayos XRESUMEN
We studied at the molecular level the interaction between neutral detergent Triton X-100 aqueous solution and a phospholipid Langmuir monolayer deposited on top using surface pressure measurement and grazing incidence X-ray diffraction (GIXD). Macroscopically, the detergent-phospholipid system follows the Gibbs law. However, GIXD shows that the detergent and the phospholipid segregate at the interface. The molecular organization of pure phospholipid domains is imposed by the detergent through surface pressure. Compression and expansion of the surface monolayer system in its final state reveal the stability of the phospholipids domains against dissolution by the detergent in the subphase, even above the detergent cmc. This resistance to dissolution is suppressed by an expansion of the monolayer.
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Octoxinol/química , Fosfolípidos/química , Tensoactivos/química , Soluciones , Agua/química , Difracción de Rayos XRESUMEN
Membrane fusion is an important process in cell biology. While the molecular mechanisms of fusion are actively studied at a very local scale, the consequences of fusion at a larger scale on the shape and stability of the membrane are still not explored. In this Letter, the evolution of the membrane tension during the fusion of positive small unilamellar vesicles with a negative giant unilamellar vesicle has been experimentally investigated and compared to an existing theoretical model. The tension has been deduced using videomicroscopy from the measurement of the fluctuation spectrum and of the time correlation function of the fluctuations. We show that fusion induces a strong decrease in the effective tension of the membrane which eventually reaches negative values. Under these conditions, we show that localized instabilities appear on the vesicle. The membrane finally collapses, forming dense lipid structures.
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Lípidos/química , Algoritmos , Vesículas Cubiertas , Membrana Dobles de Lípidos , Microscopía por Video , UltrasonidoRESUMEN
We report the first direct evidence for the formation of circular surface micelles (hemimicelles) on the surface of water. These highly monodisperse 30 nm hemimicelles, made from a semifluorinated alkane deposited as a Langmuir monolayer, form organized hexagonal arrays as determined by small-angle X-ray diffraction conducted directly on the water surface at grazing incidence.
RESUMEN
An application of the radiolysis method using an X-ray synchrotron beam is developed as a novel approach to the synthesis of metal-organic films with controlled shapes and thickness. We demonstrate that a Langmuir monolayer deposited onto a silver ion containing subphase, irradiated by an incident beam impinging below the critical angle for total reflection, induces the synthesis of a stable nanostructured silver-organic ultrathin film at the air-water interface. The X-ray scattering is also used to monitor in situ the structure of the silver layer during the synthesis process. The layer is observed by atomic force microscopy after its transfer onto a silicon substrate. One observes a film thickness of 4.6 nm, in good agreement with the X-ray penetration depth, about 4.5 nm. The silver structure is oriented by the initial organic film phase. This experiment demonstrates the considerable potential of this approach to produce various controlled metal-organic films with a surfactant self-assembly as a template.