RESUMEN
The modern cephalopod mollusks (coleoids) are considered the most behaviorally advanced invertebrate, yet little is known about the neurophysiological basis of their behaviors. Previous work suggested that the vertical lobe (VL) of cephalopods is a crucial site for the learning and memory components of these behaviors. We are therefore studying the neurophysiology of the VL in Octopus vulgaris and have discovered a robust activity-dependent long-term potentiation (LTP) of the synaptic input to the VL. Moreover, we have shown that the VL and its LTP are involved in behavioral long-term memory acquisition. To advance our understanding of the VL as a learning neural network we explore the possible involvement of neuromodulation in VL function. Here we examine whether the well studied serotonergic modulation in simple models of learning in gastropods mollusks is conserved in the octopus VL. We demonstrate histochemically that the VL is innervated by afferent terminals containing 5-HT immunoreactivity (5-HT-IR). Physiologically, 5-HT has a robust facilitatory effect on synaptic transmission and activity-dependent LTP induction. These results suggest that serotonergic neuromodulation is a part of a reinforcing/reward signaling system conserved in both simple and complex learning systems of mollusks. However, there are notable functional differences. First, the effective concentration of 5-HT in the VL is rather high (100 microM); secondly, only neuropilar regions but not cell bodies in the VL are innervated by terminals containing 5-HT-IR. Thirdly, repetitive or long exposures to 5-HT do not lead to a clear long-term facilitation. We propose that in the octopus VL, while the basic facilitatory properties of molluscan 5-HT system are conserved, the system has adapted to convey signals from other brain areas to reinforce the activity-dependent associations at specific sites in the large connections matrix in the VL.
Asunto(s)
Encéfalo/fisiología , Potenciación a Largo Plazo/fisiología , Memoria/fisiología , Octopodiformes/fisiología , Refuerzo en Psicología , Serotonina/fisiología , Transmisión Sináptica/fisiología , 1-Metil-3-Isobutilxantina/farmacología , Vías Aferentes/efectos de los fármacos , Vías Aferentes/fisiología , Animales , Encéfalo/efectos de los fármacos , Química Encefálica , AMP Cíclico/fisiología , GMP Cíclico/fisiología , Relación Dosis-Respuesta a Droga , Potenciación a Largo Plazo/efectos de los fármacos , Memoria/efectos de los fármacos , Neurópilo/efectos de los fármacos , Técnicas de Cultivo de Órganos , Serotonina/farmacología , Transmisión Sináptica/efectos de los fármacosRESUMEN
The opener muscle of the dactyl of the walking leg of crayfish is innervated by one excitatory axon releasing glutamate and one inhibitory axon releasing GABA. Functional GABA(A) receptors are present postsynaptically on the muscle and presynaptically on terminals and release boutons of the excitatory axon, whereas presynaptic GABA(A) autoreceptors have not been reported on terminals or release boutons of the inhibitory axon. Using antibodies raised against mammalian GABA(A) receptor subunits alpha3 and beta(2/3), we obtained highly specific staining of the presynaptic membrane of the inhibitory bouton and of the postsynaptic membrane of the muscle. Using pre- and postembedding techniques, staining was localized to only presynaptic and postsynaptic membranes of synaptic active zones. We also found extrasynaptic receptor subunit immunoreactivity near (up to 100 nm) to the active zones. Staining with antibodies for the alpha3 and beta(2/3) subunits showed colocalization of particles of the two subunits. We suggest that presynaptic inhibitory boutons of the crayfish possess GABA(A)-like autoreceptors composed of at least the alpha3 and beta(2/3) subunits.
Asunto(s)
Astacoidea/fisiología , Músculo Esquelético/inervación , Músculo Esquelético/metabolismo , Terminales Presinápticos/metabolismo , Receptores de GABA-A/biosíntesis , Animales , Anticuerpos Monoclonales , Membrana Celular/inmunología , Membrana Celular/metabolismo , Membrana Celular/ultraestructura , Estimulación Eléctrica , Substitución por Congelación , Inmunohistoquímica , Microscopía Electrónica , Músculo Esquelético/ultraestructura , Inhibición Neural/fisiología , Unión Neuromuscular/metabolismo , Unión Neuromuscular/ultraestructura , Terminales Presinápticos/ultraestructura , Subunidades de Proteína/biosíntesis , Subunidades de Proteína/inmunología , Receptores de GABA-A/inmunologíaRESUMEN
The purpose of this pilot study was to evaluate the effectiveness of a parent-focused intervention program (COPE) on infant cognitive development and maternal coping. A randomized clinical trial was conducted with 42 mothers of low-birth-weight (LBW) premature infants hospitalized in a neonatal intensive care unit (NICU), with follow-up at 3 months' and 6 months' corrected ages. COPE mothers received the four-phase educational-behavioral program that began 2-4 days postbirth and continued through 1 week following discharge from the NICU. Comparison mothers received audiotaped information during the same four time frames. Results indicated that COPE infants had significantly higher mental development scores at a 3 months' corrected age (M = 100.3) than did the comparison infants (M = 93.9), and this difference widened at 6 months' corrected age, with COPE infants scoring 14 points higher. COPE mothers were significantly less stressed by the NICU sights and sounds and had significantly stronger beliefs about what behaviors and characteristics to expect from their premature infants. Findings from this study support the need for further testing of early NICU interventions with parents to determine their effectiveness on parental coping and infant developmental outcomes.
Asunto(s)
Adaptación Psicológica , Desarrollo Infantil , Cognición , Recién Nacido de Bajo Peso , Relaciones Madre-Hijo , Educación del Paciente como Asunto , Adolescente , Adulto , Niño Hospitalizado , Femenino , Humanos , Recién Nacido , Unidades de Cuidado Intensivo Neonatal , Entrevistas como Asunto , Masculino , Proyectos PilotoRESUMEN
The purpose of this study was to determine whether maternal anxiety and mothers' participation in their children's care during hospitalization mediated the effects of a child behavior informational intervention for mothers on their children's posthospital negative behavioral change. Participants were 49 mothers and their young children, ages 24-68 months, who were unexpectedly hospitalized with unplanned medical or surgical conditions. These participants were drawn from a larger study of the separate and combined effects of child behavior information and parent role information on the process and outcomes of maternal and child coping with unplanned hospitalization. Findings indicated that the effects of child behavior information on children's posthospital negative behavioral change were mediated by maternal anxiety and participation in their children's care during hospitalization. Results of this study provide support for targeting mothers with informational interventions in order to enhance outcomes in hospitalized children.
Asunto(s)
Adaptación Psicológica , Ansiedad/psicología , Trastornos de la Conducta Infantil/etiología , Trastornos de la Conducta Infantil/psicología , Conducta Infantil/psicología , Niño Hospitalizado/psicología , Educación en Salud/organización & administración , Madres/educación , Madres/psicología , Adulto , Ansiedad/diagnóstico , Ansiedad/prevención & control , Trastornos de la Conducta Infantil/prevención & control , Preescolar , Femenino , Humanos , Lactante , Masculino , Evaluación de Procesos y Resultados en Atención de Salud , Responsabilidad Parental , Análisis de Regresión , Encuestas y CuestionariosRESUMEN
This article describes the methods used and findings obtained in establishing the HIV/AIDS Prevention Program Archive (HAPPA), a collection of effective HIV/AIDS prevention programs in a box. The HAPPA collection builds on a previously established collection of 13 effective HIV/AIDS/sexually transmitted disease (STD) prevention programs for adolescents known as PASHA, the Program Archive on Sexuality, Health, & Adolescence. Together, the HAPPA and PASHA collections provide a rich source of 23 promising programs designed to prevent the spread of HIV/AIDS. The HAPPA and PASHA programs are available for use by communities, schools, family planning clinics, STD clinics, mental health centers, and drug rehabilitation centers throughout the country.
Asunto(s)
Infecciones por VIH/prevención & control , Educación en Salud/organización & administración , Prevención Primaria/organización & administración , Desarrollo de Programa/métodos , Síndrome de Inmunodeficiencia Adquirida/epidemiología , Síndrome de Inmunodeficiencia Adquirida/prevención & control , Femenino , Infecciones por VIH/epidemiología , Conocimientos, Actitudes y Práctica en Salud , Humanos , Masculino , Evaluación de Programas y Proyectos de Salud/normas , Abuso de Sustancias por Vía IntravenosaRESUMEN
Focus groups can be used to gather rich, detailed descriptions of shared individual experiences and beliefs. Group process enhances the richness of the data obtained via this method. Nurses are skilled in gathering detailed and often sensitive information and applying therapeutic communication and interviewing techniques within groups. They can take advantage of these skills by using focus groups to collect qualitative data. To maximize the collection of high-quality data, pay specific attention to the selection and training of the moderator, the development of the interview guide, and the analysis that addresses intragroup and intergroup processes.
Asunto(s)
Grupos Focales/métodos , Interpretación Estadística de Datos , Procesos de Grupo , Humanos , Relaciones Interpersonales , Entrevistas como Asunto/métodos , Proyectos de InvestigaciónRESUMEN
Estimates indicate that approximately 31% of children are affected by one or more chronic illnesses. Furthermore, caring for a chronically ill child imposes a host of long-term stressors for parents that need to be addressed by sensitive, evidence-based interventions. This article will: (a) review stressors of childhood chronic illness for parents over time; (b) provide a summary of tools that can be used to assess parental coping, (c) delineate important nursing assessments, (d) review interventions that have resulted in improved parental coping outcomes, and (e) describe a theoretical framework that can be used to assess and intervene with parents of chronically ill children.
Asunto(s)
Adaptación Psicológica , Enfermedad Crónica/psicología , Padres/psicología , Niño , Enfermedad Crónica/enfermería , Humanos , Evaluación en Enfermería , Relaciones Profesional-FamiliaRESUMEN
Intermittent auscultation (IA) has been reported as equivalent to electronic fetal monitoring (EFM) as a fetal surveillance method in terms of neonatal outcomes based on randomized controlled trials and meta-analyses. Despite recommendations to include IA as a primary method for fetal evaluation, EFM use predominates. Understanding the equipment, method, benefits and limitations, and strategies for implementing IA may assist nurses in providing informed choices for low-risk pregnant women.
Asunto(s)
Monitoreo Fetal/métodos , Auscultación Cardíaca/métodos , Frecuencia Cardíaca Fetal , Evaluación en Enfermería/métodos , Algoritmos , Femenino , Monitoreo Fetal/enfermería , Auscultación Cardíaca/instrumentación , Auscultación Cardíaca/enfermería , Humanos , EmbarazoRESUMEN
In heat-acclimated rock pigeons, cutaneous water evaporation is the major cooling mechanism when exposed at rest to an extremely hot environment of 50-60 degrees C. This evaporative pathway is also activated in room temperature by a beta-adrenergic antagonist (propranolol) or an alpha-adrenergic agonist (clonidine) and inhibited by a beta-adrenergic agonist (isoproterenol). In contrast, neither heat exposure nor drug administration activates cutaneous evaporation in cold-acclimated pigeons. To elucidate the mechanisms underlying this phenomenon, we studied the role of the ultrastructure and permeability of the cutaneous vasculature. During both heat stress and the administration of propranolol and clonidine, we observed increased capillary fenestration and endothelial gaps. Similarly, propranolol increased the extravasation of Evans blue-labeled albumin in the skin tissue. We concluded that heat acclimation reinforces a mechanism by which the activation of adrenergic signal transduction pathways alters microvessel permeability during heat stress. Consequently the flux of plasma proteins and water into the interstitial space is accelerated, providing an interstitial source of water for sustained cutaneous evaporative cooling.
Asunto(s)
Aclimatación/fisiología , Columbidae/fisiología , Trastornos de Estrés por Calor/patología , Calor , Piel/irrigación sanguínea , Animales , Temperatura Corporal/fisiología , Capilares/metabolismo , Capilares/ultraestructura , Permeabilidad Capilar , Colorantes/farmacocinética , Columbidae/anatomía & histología , Azul de Evans/farmacocinética , Trastornos de Estrés por Calor/fisiopatología , Microscopía Electrónica , Piel/metabolismo , Pérdida Insensible de AguaRESUMEN
Functional and immunocytochemical identification of glutamate autoreceptors of an NMDA type in crayfish neuromuscular junction. J. Neurophysiol. 80: 2893-2899, 1998. N-Methyl--aspartate (NMDA) reduces release from crayfish excitatory nerve terminals. We show here that polyclonal and monoclonal antibodies raised against the mammalian postsynaptic NMDA receptor subunit 1 stain specifically the presynaptic membrane of release boutons of the crayfish neuromuscular junction. In crayfish ganglionic membranes, the polyclonal antibody recognizes a single protein band that is somewhat larger (by approximately 30 kD) than the molecular weight of the rat receptor. Moreover, the monoclonal (but not the polyclonal) antibody abolishes the physiological effect of NMDA on glutamate release. The monoclonal antibody did not prevent the presynaptic effects of glutamate, which also reduces release by activation of quisqualate presynaptic receptors. Only when 6-cyano-7-nitroquinoxatine-2,3,dione (CNQX) was added together with the monoclonal antibody was the presynaptic effect of glutamate blocked. These results show that presynaptic glutamate receptors of the crayfish NMDA type are involved in the regulation of neurotransmitter release in crayfish axon terminals. Although the crayfish receptor differs in its properties from the mammalian NMDA receptor, the two receptors retained some structural similarity.
Asunto(s)
Astacoidea/fisiología , Autorreceptores/metabolismo , Unión Neuromuscular/fisiología , Receptores de N-Metil-D-Aspartato/metabolismo , Animales , Anticuerpos Bloqueadores/farmacología , Autorreceptores/efectos de los fármacos , Autorreceptores/ultraestructura , Western Blotting , Electrofisiología , Agonistas de Aminoácidos Excitadores/farmacología , Inmunohistoquímica , Técnicas In Vitro , Microscopía Electrónica , Músculos/citología , Músculos/efectos de los fármacos , Músculos/metabolismo , N-Metilaspartato/farmacología , Unión Neuromuscular/efectos de los fármacos , Unión Neuromuscular/ultraestructura , Terminales Presinápticos/efectos de los fármacos , Terminales Presinápticos/fisiología , Ratas , Receptores de N-Metil-D-Aspartato/efectos de los fármacos , Receptores de N-Metil-D-Aspartato/ultraestructuraRESUMEN
Peripheral axons of lobsters can survive for many months after axotomy. We have investigated the structural and ultrastructural changes seen after axotomy using confocal microscopy and electron microscopy. While the proximal stump had a normal appearance, the distal part of the cut axon became lobulated, and glial cells penetrated the original glial tube (axon tube) in which the axon normally runs. The changes proceeded from the cut end towards the muscle. As time elapsed, the axon tube seemed to be filled with glial cells, but interposed small profiles of the original axon could be identified by injection of a fluorescent dye into the axon. The glial cells send cytoplasmic projections deep into folds of the axolemma, and nuclei were found at the end of these long processes. Proliferation of glial cells was also seen.
Asunto(s)
Axones/fisiología , Nephropidae/anatomía & histología , Nephropidae/fisiología , Abdomen/inervación , Animales , Axotomía , Microscopía Confocal , Microscopía Electrónica , Neuroglía/fisiología , Neuroglía/ultraestructuraRESUMEN
The muscarinic acetylcholine receptors are important in a variety of physiological processes such as induction of secretion from various glands and regulation of pacemaker activity, muscle tone, and neurotransmission. To date, the muscarinic receptor family includes five members (designated m1-m5), of which m1-m4 are abundant in brain and in peripheral tissues, and m5 is found exclusively in brain, and even there at very low levels. The expression of m1-m5 receptor subtypes was studied in neurons derived from the murine embryonal carcinoma cell line P19. These cells serve as a model system for differentiation and maturation of neurons resembling CNS neurons. Our results show that P19 neurons express mainly the m2, m3, and m5 subtypes. Low levels of m1 receptors are also detected and m4 subtype is practically absent. Furthermore, muscarinic receptors in P19 neurons are functional in activating second messenger signaling pathways. The localization of m2 receptors is predominantly presynaptic, whereas the m5 subtype is mainly postsynaptic. Consequently, P19 cells provide a model system for the study of pre- and postsynaptic muscarinic acetylcholine-receptor subtypes in a proper neuronal context. This is particularly valid for the rare m5 receptors.
Asunto(s)
Neuronas/metabolismo , Receptores Muscarínicos/biosíntesis , Receptores Muscarínicos/metabolismo , Inhibidores de Adenilato Ciclasa , Animales , Carcinoma Embrionario , AMP Cíclico/antagonistas & inhibidores , Activación Enzimática/efectos de los fármacos , Inmunohistoquímica , Ratones , Antagonistas Muscarínicos/metabolismo , N-Metilescopolamina/metabolismo , Neuronas/patología , Neuronas/ultraestructura , Receptor Muscarínico M2 , Receptor Muscarínico M4 , Receptores Muscarínicos/fisiología , Fracciones Subcelulares/metabolismo , Fracciones Subcelulares/ultraestructura , Células Tumorales Cultivadas , Fosfolipasas de Tipo C/metabolismoRESUMEN
A Drosophila cell-free system was used to characterize proteins that are required for targeting vesicles to chromatin and for fusion of vesicles to form nuclear envelopes. Treatment of vesicles with 1 M NaCl abolished their ability to bind to chromatin. Binding of salt-treated vesicles to chromatin could be restored by adding the dialyzed salt extract. Lamin Dm is one of the peripheral proteins whose activity was required, since supplying interphase lamin isoforms Dm1, and Dm2 to the assembly extract restored binding. As opposed to the findings in Xenopus, okadaic acid had no effect on vesicle binding. Trypsin digestion of the salt-stripped vesicles eliminated their association with chromatin even in the presence of the dialyzed salt extract. One vesicles attached to chromatin surface, fusion events took place were found to be sensitive to guanosine 5'-[gamma-thio]triphosphate (GTP gamma S). These chromatin-attached vesicles contained lamin Dm and otefin but not gp210. Thus, these results show that in Drosophila there are two populations of nuclear vesicles. The population that interacts first with chromatin contains lamin and otefin and requires both peripheral and integral membrane proteins, whereas fusion of vesicles requires GTPase activity.
Asunto(s)
Cromatina/metabolismo , Guanosina 5'-O-(3-Tiotrifosfato)/farmacología , Proteínas de la Membrana/metabolismo , Membrana Nuclear/metabolismo , Animales , Sistema Libre de Células , Cromatina/efectos de los fármacos , Cromatina/ultraestructura , Drosophila , Laminina/efectos de los fármacos , Proteínas de la Membrana/efectos de los fármacos , Microscopía Electrónica , Ácido Ocadaico/farmacologíaRESUMEN
Synaptotagmins are a gene family of membrane proteins with distinct expression patterns. Synaptotagmin I is an abundant protein of the synaptic vesicle membrane and was implicated as the Ca2+ sensor in fast responding synapses. Yet, its precise role along the synaptic vesicle life cycle is not fully understood. In this report we show that synaptotagmin I is not exclusively confined to neuronal and neuroendocrine systems, rather, it is also expressed in the exocrine system of the parotid gland. The gene for synaptotagmin I was isolated and sequenced from rat parotid cDNA. The identity of synaptotagmin I protein was further confirmed by several independent antibodies. The protein is exclusively found in the membranous fraction of purified granules, similarly to VAMP-2, another major integral membrane protein of synaptic vesicles. Synaptotagmin I represents 0.4% of the total membrane protein mass of the granule. Using immunoelectron microscopy the two proteins were also localized primarily to the granules' membranes. These findings suggest that synaptotagmin I which regulates Ca(2+)-dependent neurotransmitter release also plays a role which is common to all secretory organelles-neuronal, endocrine and exocrine. A role for synaptotagmin I in integrating signals with protein secretion in the parotid gland is suggested.
Asunto(s)
Proteínas de Unión al Calcio , Glicoproteínas de Membrana/biosíntesis , Glicoproteínas de Membrana/metabolismo , Proteínas del Tejido Nervioso/biosíntesis , Proteínas del Tejido Nervioso/metabolismo , Glándula Parótida/metabolismo , Animales , Gránulos Citoplasmáticos/química , Gránulos Citoplasmáticos/metabolismo , Gránulos Citoplasmáticos/ultraestructura , Precursores Enzimáticos/química , Precursores Enzimáticos/ultraestructura , Membranas Intracelulares/enzimología , Membranas Intracelulares/ultraestructura , Masculino , Glicoproteínas de Membrana/genética , Proteínas de la Membrana/química , Proteínas de la Membrana/aislamiento & purificación , Datos de Secuencia Molecular , Proteínas del Tejido Nervioso/genética , Glándula Parótida/citología , Glándula Parótida/ultraestructura , Proteínas R-SNARE , ARN Mensajero/química , ARN Mensajero/aislamiento & purificación , Ratas , Ratas Sprague-Dawley , Sinaptotagmina I , Sinaptotagminas , Transcripción GenéticaRESUMEN
Otefin is a 45-kDa nuclear envelope protein with no apparent homology to other known proteins. It includes a large hydrophilic domain, a single carboxyl-terminal hydrophobic sequence of 17 amino acids, and a high content of serine and threonine residues. Cytological labeling located otefin on the nucleoplasmic side of the nuclear envelope. Chemical extraction of nuclei from Drosophila embryos revealed that otefin is a peripheral protein whose association with the nuclear envelope is stronger than that of lamin. Deletion mutants of otefin were expressed in order to identify regions that direct otefin to the nuclear envelope. These experiments revealed that the hydrophobic sequence at the carboxyl terminus is essential for correct targeting to the nuclear envelope, whereas additional regions in the hydrophilic domain of otefin are required for its efficient targeting and stabilization in the nuclear envelope.
Asunto(s)
Proteínas de la Membrana/metabolismo , Membrana Nuclear/metabolismo , Proteínas Nucleares/metabolismo , Animales , Células COS , Drosophila , Proteínas de Drosophila , Operón Lac , Microscopía Electrónica , Microscopía Inmunoelectrónica , Peso Molecular , Mutagénesis Sitio-Dirigida , Membrana Nuclear/ultraestructura , Conformación Proteica , Eliminación de Secuencia , Solubilidad , Relación Estructura-Actividad , TransfecciónRESUMEN
In this report we show that alpha-latrotoxin from black widow spider venom is a potent activator of neurotransmitter release in synaptosomes from the Torpedo electric organ. Binding of the purified toxin (5 nM) to the synaptosomal fraction occurs already at 4 degrees C and is dependent on the presence of divalent ions. However, neurotransmitter release commences only after temperature elevation (22 degrees C) and is completed within 2 min. The effect of alpha-latrotoxin on release is achieved at 1 nM and is already saturated at 5 nM. The release is stimulated by the presence of Ca2+ ions. Activation of release by alpha-latrotoxin is accompanied by morphological changes in electric organ synaptosomes. The synaptosomes swell, resulting in a 55% increase in section area. Moreover, the number of synaptic vesicles per unit area decreases about three-fold, and rows of docked synaptic vesicles are rarely detected as opposed to control synaptosomes. These morphological changes indicate that the massive release is mainly due to synaptic vesicle fusion. alpha-Latrotoxin binding sites are highly concentrated in the innervated face of the electrocytes. Immunoelectron microscopy on electric organ sections reveals alpha-latrotoxin binding sites over the entire plasma membrane at release sites and facing Schwann cells surrounding Torpedo nerve terminals. Surprisingly, a high concentration of binding sites is also found at structures surrounding branching unmyelinated axons. This staining is in close proximity to Schwann cell envelopes and to the basal lamina around axonal tips. The mode of action of alpha-latrotoxin in view of the localization of its binding sites is discussed.