RESUMEN
The biotrophic fungal pathogen Blumeria graminis f. sp. tritici (Bgt) is a crucial factor causing reduction in global wheat production. Wild wheat relatives, for example Thinopyrum intermedium, is one of the wild-used parents in wheat disease-resistant breeding. From T. intermedium line, we identified the aspartic protease gene, TiAP1, which is involved in resistance against Bgt. TiAP1 is a secreted protein that accumulates in large amounts at the infection sites of Bgt and extends to the intercellular space. Yeast two-hybrid, luciferase complementation imaging and bimolecular florescent complimentary analysis showed that TiAP1 interacted with the chitin deacetylase (BgtCDA1) of Bgt. The yeast expression, purification and in vitro test confirmed the chitin deacetylase activity of BgtCDA1. The bombardment and VIGS-mediated host-induced gene silencing showed that BgtCDA1 promotes the invasion of Bgt. Transcriptome analysis showed the cell wall xylan metabolism, lignin biosynthesis-related and defence genes involved in the signal transduction were up-regulated in the transgenic TiAP1 wheat induced by Bgt. The TiAP1 in wheat may inactivate the deacetylation function of BgtCDA1, cause chitin oligomers expose to wheat chitin receptor, then trigger the wheat immune response to inhibit the growth and penetration of Bgt, and thereby enhance the resistance of wheat to pathogens.
Asunto(s)
Enfermedades de las Plantas , Triticum , Amidohidrolasas , Ascomicetos , Quitina/metabolismo , Resistencia a la Enfermedad/genética , Fitomejoramiento , Enfermedades de las Plantas/microbiología , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Saccharomyces cerevisiae , Triticum/metabolismoRESUMEN
Auxin is an important endogenous hormone in plants. The YUCCA gene encodes a flavin monooxygenase, which is an important rate-limiting enzyme in the auxin synthesis pathway and involved in the regulation of plant growth and development. In the study, we identified 63 wheat TaYUCCA genes; among them, some genes appeared in clusters. By constructing phylogenetic trees, we found that the TaYUCCA genes could be divided into six groups. In the WheatExp database, there were 22 differential expressed TaYUCCA genes, among which the TaYUCCA10 gene was abundantly expressed in the endosperm and medium milk stage, the TaYUCCA2 gene was abundantly expressed in the roots of three leaves and meiosis and transfer cells at 20 days post anthesis and the others 16 TaYUCCA genes had different expression level at different developmental stages in wheat, and there were 15 TaYUCCA genes induced by drought and heat stress, among which the TaYUCCA2-D, TaYUCCA3-B, and TaYUCCA9-D might be upregulated induced by drought stress, TaYUCCA10.1 might be upregulated induced drought and heat stress, TaYUCCA6-A was upregulated induced both drought and heat stress and the others 9 TaYUCCA genes were downregulated induced by drought and heat stress. Transcriptome and qRT-PCR analysis showed that TaYUCCA7-A was upregulated significantly after induced by powdery mildew. The comprehensive annotation and expression profiling of the TaYUCCA genes in this study enhanced our understanding of TaYUCCA family gene expression in wheat growth and development and laid the foundation for the further study of TaYUCCA gene mechanism.
Asunto(s)
Hojas de la Planta/genética , Proteínas de Plantas/genética , Transcriptoma/genética , Triticum/genética , Sequías , Regulación de la Expresión Génica de las Plantas/genética , Genoma de Planta/genética , Ácidos Indolacéticos/metabolismo , Familia de Multigenes/genética , Filogenia , Reguladores del Crecimiento de las Plantas , Hojas de la Planta/crecimiento & desarrollo , Raíces de Plantas , Estrés Fisiológico/genética , Triticum/crecimiento & desarrolloRESUMEN
KEY MESSAGE: Present study revealed that specific expression of TaYUC10.3 in wheat young seeds could increase the content of auxin, and protein. Auxin is a vital endogenous hormone in plants, which is involved in the regulation of various physiological and biochemical processes in plants. The flavin-containing monooxygenase encoded by the YUCCA gene is a rate-limiting enzyme in the tryptophan-dependent pathway of auxin synthesis. TaYUC10.3 was identified, cloned and found that it was abundantly expressed in wheat young seeds. In this study, a seed-specific expression vector of TaYUC10.3 was constructed with the promoter of 1Bx17 glutenin subunit gene and transformed wheat using the particle bombardment method. The quantitative RT-PCR showed that TaYUC10.3 was expressed in a large amount in young seeds of the transgenic lines. Plant hormone-targeted metabolomics showed that the auxin content of the transgenic lines was significantly increased compared with controls. The GC / MS non-targeted metabolite multiple statistical analyses showed that the variable importance in projection (VIP) of tryptophan reduced in the transgenic lines. Simultaneously, the VIP of indole acetic acid increased. The precursor amino acids for synthesizing some proteins and carbohydrates were upregulated in the transgenic lines. Subsequently, it was found that the protein content of the seeds of the transgenic TaYUC10.3 wheat was significantly higher than that of the control. The wet gluten content and sedimentation value of the transgenic TaYUC10.3 wheat were also high. This result indicated that TaYUC10.3 might participate in auxin synthesis and affects the protein content of wheat seeds.
Asunto(s)
Ácidos Indolacéticos/metabolismo , Reguladores del Crecimiento de las Plantas/metabolismo , Proteínas de Plantas/metabolismo , Triticum/genética , Regulación de la Expresión Génica de las Plantas , Glútenes/análisis , Glútenes/genética , Glútenes/metabolismo , Especificidad de Órganos , Proteínas de Plantas/genética , Plantas Modificadas Genéticamente , Regiones Promotoras Genéticas/genética , Semillas/genética , Semillas/metabolismo , Triticum/metabolismoRESUMEN
Aspartic proteases (APs) are one of the four main protease super families. In plants, they are involved in many biological processes, such as biotic and abiotic stress resistance, protein processing and degradation, senescence, and programmed cell death. By performing a database (TGACv1) search and domain prediction, we identified 263 wheat AP (TaAP) proteins and observed 38 TaAP genes exhibiting alternative splicing. Moreover, by constructing a phylogenetic tree, we found that the TaAP proteins can be divided into three families and have a certain close evolutionary relationship to Arabidopsis thaliana and rice AP proteins. Transcriptome analysis showed that 29 genes in the TaAP family were up-regulated after being induced by powdery mildew. The expression of TaAP224 showed the most significant difference in transcriptome and qRT-PCR analyses. Subsequently, the promoters of these 29 genes were analysed, and we found that they contained multiple disease resistance and hormone elements, such as WRKY71OS, a common disease resistance element that is also involved in the GA signalling pathway and inhibits starch hydrolysis. The comprehensive annotation and expression profiling performed in this study increased our understanding of the TaAP family genes in wheat growth and development, and the results can be used as a basis for further study of candidate TaAP genes involved in powdery mildew resistance mechanisms.
Asunto(s)
Proteasas de Ácido Aspártico/genética , Resistencia a la Enfermedad/genética , Genoma de Planta/genética , Enfermedades de las Plantas/genética , Proteínas de Plantas/genética , Triticum/genética , Ascomicetos/fisiología , Proteasas de Ácido Aspártico/clasificación , Proteasas de Ácido Aspártico/metabolismo , Perfilación de la Expresión Génica/métodos , Regulación Enzimológica de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Interacciones Huésped-Patógeno , Isoenzimas/clasificación , Isoenzimas/genética , Isoenzimas/metabolismo , Familia de Multigenes/genética , Filogenia , Enfermedades de las Plantas/microbiología , Proteínas de Plantas/clasificación , Proteínas de Plantas/metabolismo , Triticum/enzimología , Triticum/microbiologíaRESUMEN
KEY MESSAGE: Expression of TaRUB1 gene in Arabidopsis thaliana elevates the level of disease-related genes in response to pathogen invasion through the accumulation of callose, necrotic cells, and the outbreak of ROS. Ubiquitin (Ub) and ubiquitin-like proteins are highly conserved in sequence and can covalently bind and modify many intracellular proteins which can be recognized and degraded by 26S proteasome. Post-translational modification of proteins has become a hot research spot today. In the previous study, a cDNA of related-to-ubiquitin protein belonged to ubiquitin-like proteins, whose spatial structure comprised Ub and NEDD8, was obtained from wheat SN6306 by suppression-subtractive hybridization and was named TaRUB1. TaRUB1 is induced by wheat powdery mildew and significantly upregulated in resistant wheat SN6306. In this study, heterologous expression of TaRUB1 in A. thaliana was used to study the function of this gene in response to pathogen Pseudomonas syringae pv. Tomato DC3000 (Pst DC3000). Transgenic A. thaliana showed relatively fewer disease symptoms, accompanied by common inhibition of living body parasitic defense responses, accumulation of more callose and reactive oxygen species (ROS), and concentrated cell death, simultaneously antioxidant enzyme activities of superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase were higher than those in wild-type (WT) plant after infection with Pst DC3000. Meanwhile, hypersensitive cell death, which was possibly ROS burst, was also observed in transgenic A. thaliana. By quantitative reverse transcription-polymerase chain reaction analysis, some marker genes for hypersensitive response showed significantly higher transcriptional expression level in transgenic A. thaliana, which activates system-acquired resistance, than that of WT plants. Heterologous expression of TaRUB1 can significantly enhance resistance to Pst DC3000 in A. thaliana, suggesting that TaRUB1 is related to plant disease resistance.
Asunto(s)
Arabidopsis/metabolismo , Plantas Modificadas Genéticamente/metabolismo , Plantas Modificadas Genéticamente/microbiología , Triticum/metabolismo , Triticum/microbiología , Arabidopsis/genética , Arabidopsis/microbiología , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Resistencia a la Enfermedad/genética , Resistencia a la Enfermedad/fisiología , Regulación de la Expresión Génica de las Plantas , Enfermedades de las Plantas/genética , Enfermedades de las Plantas/microbiología , Plantas Modificadas Genéticamente/genética , Procesamiento Proteico-Postraduccional , Pseudomonas syringae/patogenicidad , Especies Reactivas de Oxígeno/metabolismo , Triticum/genética , Ubiquitinas/metabolismoRESUMEN
Wheat powdery mildew, which is mainly caused by Blumeria graminis f. sp. tritici (Bgt), seriously damages wheat production. The wheat-Thinopyrum intermedium alien addition disomic line germplasm SN6306, being one of the important sources of genes for wheat resistance, is highly resistant to Bgt E09 and to many other powdery mildew physiological races. However, knowledge on the resistance mechanism of SN6306 remains limited. Our study employed high-throughput RNA sequencing based on next-generation sequencing technology (Illumina) to obtain an overview of the transcriptome characteristics of SN6306 and its parent wheat Yannong 15 (YN15) during Bgt infection. The sequencing generated 104,773 unigenes, 9909 of which showed varied expression levels. Among the 9909 unigenes, 1678 unigenes showed 0 reads in YN15. The expression levels in Bgt-inoculated SN6306 and YN15 of exactly 39 unigenes that showed 0 or considerably low reads in YN15 were validated to identify the genes involved in Bgt resistance. Among the 39 unigenes, 12 unigenes were upregulated in SN6306 by 3-45 times. These unigenes mainly encoded kinase, synthase, proteases, and signal transduction proteins, which may play an important role in the resistance against Bgt. To confirm whether the unigenes that showed 0 reads in YN15 are really unique to SN6306, 8 unigenes were cloned and sequenced. Results showed that the selected unigenes are more similar to SN6306 and Th. intermedium than to the wheat cultivar YN15. The sequencing results further confirmed that the unigenes showing 0 reads in YN15 are unique to SN6306 and are most likely derived from Th. intermedium (Host) Nevski. Thus, the genes from Th. intermedium most probably conferred the resistance of SN6306 to Bgt.
Asunto(s)
Resistencia a la Enfermedad/genética , Enfermedades de las Plantas/genética , Proteínas de Plantas/genética , Transcriptoma/inmunología , Triticum/genética , Ascomicetos/patogenicidad , Ascomicetos/fisiología , Mapeo Cromosómico , Cromosomas de las Plantas/química , Resistencia a la Enfermedad/inmunología , Perfilación de la Expresión Génica , Biblioteca de Genes , Ontología de Genes , Secuenciación de Nucleótidos de Alto Rendimiento , Interacciones Huésped-Patógeno , Anotación de Secuencia Molecular , Enfermedades de las Plantas/inmunología , Enfermedades de las Plantas/microbiología , Inmunidad de la Planta/genética , Proteínas de Plantas/inmunología , Triticum/inmunología , Triticum/microbiologíaRESUMEN
YUCCA protein participates in a key rate-limiting step in the tryptophan-dependent pathway for auxin biosynthesis and is involved in numerous processes during plant development. In this study, the genomic and cDNA sequences of three TaYUC10 homoeologous genes were isolated. These sequences showed a very high conservation in coding region and the exon/intron structure, whereas their intron lengths were different. The cDNA and polypeptide chains of the three TaYUC10 genes were highly similar. These genes were most homologous to BdYUC10. Location analysis showed that TaYUC10.1 was present in chromosome 5BL. TaYUC10.3 was expressed in all parts of the wheat, but was predominant in the reproductive organs of mature wheat, such as flowering spikelets or fertilized embryos. In the fertilized embryos 28d post-anthesis, expression of TaYUC10.3 was clearly increased with the development of seeds. This indicates that TaYUC genes may play a vital role in seed development. TaYUC10.3 overexpressed in Arabidopsis had a typical phenotype, excessive auxin accumulation also seen in higher plants, and showed increased spacing of silique and downward curling of the blade margin. Sterility was observed in adult transgenic plants, becoming more severe in late development. The floral structures of sterile plants were not integrated. TaYUC10 may be required for numerous wheat growth processes, including flower and seed development.
Asunto(s)
Genes de Plantas/fisiología , Semillas/genética , Triticum/genética , Arabidopsis/genética , Arabidopsis/metabolismo , Cromosomas de las Plantas/genética , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/metabolismoRESUMEN
Grain protein content in wheat (Triticum aestivum L.) is generally considered a highly heritable character that is negatively correlated with grain yield and yield-related traits. Quantitative trait loci (QTL) for protein content was mapped using data on protein content and protein content conditioned on the putatively interrelated traits to evaluate possible genetic interrelationships between protein content and yield, as well as yield-related traits. Phenotypic data were evaluated in a recombinant inbred line population with 302 lines derived from a cross between the Chinese cultivar Weimai 8 and Luohan 2. Inclusive composite interval mapping using IciMapping 3.0 was employed for mapping unconditional and conditional QTL with additives. A strong genetic relationship was found between protein content and grain yield, and yield-related traits. Unconditional QTL mapping analysis detected seven additive QTL for protein content, with additive effects ranging in absolute size from 0.1898% to 0.3407% protein content, jointly accounting for 43.45% of the trait variance. Conditional QTL mapping analysis indicated two QTL independent from yield, which can be used in marker-assisted selection for increasing yield without affecting grain protein content. Three additional QTL with minor effects were identified in the conditional mapping. Of the three QTLs, two were identified when protein content was conditioned on yield, which had pleiotropic effects on those two traits. Conditional QTL mapping can be used to dissect the genetic interrelationship between two traits at the individual QTL level for closely correlated traits. Further, conditional QTL mapping can reveal additional QTL with minor effects that are undetectable in unconditional mapping.
Asunto(s)
Mapeo Cromosómico/métodos , Grano Comestible/crecimiento & desarrollo , Proteínas de Plantas/metabolismo , Sitios de Carácter Cuantitativo , Cromosomas de las Plantas/genética , Grano Comestible/genética , Grano Comestible/metabolismo , Reproducibilidad de los Resultados , Triticum/genética , Triticum/crecimiento & desarrollo , Triticum/metabolismoRESUMEN
Arabidopsis cryptochrome 2 (CRY2) is a blue-light receptor mediating blue-light inhibition of hypocotyl elongation and photoperiodic promotion of floral initiation. CRY2 is a constitutive nuclear protein that undergoes blue-light-dependent phosphorylation, ubiquitination, photobody formation, and degradation in the nucleus, but the relationship between these blue-light-dependent events remains unclear. It has been proposed that CRY2 phosphorylation triggers a conformational change responsible for the subsequent ubiquitination and photobody formation, leading to CRY2 function and/or degradation. We tested this hypothesis by a structure-function study, using mutant CRY2-GFP fusion proteins expressed in transgenic Arabidopsis. We show that changes of lysine residues of the NLS (Nuclear Localization Signal) sequence of CRY2 to arginine residues partially impair the nuclear importation of the CRY2K541R and CRY2K554/5R mutant proteins, resulting in reduced phosphorylation, physiological activities, and degradation in response to blue light. In contrast to the wild-type CRY2 protein that forms photobodies exclusively in the nucleus, the CRY2K541R and CRY2K554/5R mutant proteins form protein bodies in both the nucleus and cytosol in response to blue light. These results suggest that photoexcited CRY2 molecules can aggregate to form photobody-like structure without the nucleus-dependent protein modifications or the association with the nuclear CRY2-interacting proteins. Taken together, the observation that CRY2 forms photobodies markedly faster than CRY2 phosphorylation in response to blue light, we hypothesize that the photoexcited cryptochromes form oligomers, preceding other biochemical changes of CRY2, to facilitate photobody formation, signal amplification, and propagation, as well as desensitization by degradation.
Asunto(s)
Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Arabidopsis/efectos de la radiación , Criptocromos/química , Criptocromos/metabolismo , Luz , Proteolisis/efectos de la radiación , Secuencia de Aminoácidos , Arabidopsis/genética , Arabidopsis/crecimiento & desarrollo , Núcleo Celular/metabolismo , Núcleo Celular/efectos de la radiación , Hipocótilo/crecimiento & desarrollo , Hipocótilo/efectos de la radiación , Lisina/metabolismo , Datos de Secuencia Molecular , Proteínas Mutantes/química , Proteínas Mutantes/metabolismo , Mutación/genética , Señales de Localización Nuclear/química , Señales de Localización Nuclear/metabolismo , Fosforilación/efectos de la radiación , Plantas Modificadas Genéticamente , Estructura Cuaternaria de Proteína , Transporte de Proteínas/efectos de la radiaciónRESUMEN
Plant height (PH) in wheat is a complex trait; its components include spike length (SL) and internode lengths. To precisely analyze the factors affecting PH, two F(8:9) recombinant inbred line (RIL) populations comprising 485 and 229 lines were generated. Crosses were performed between Weimai 8 and Jimai 20 (WJ) and between Weimai 8 and Yannong 19 (WY). Possible genetic relationships between PH and PH components (PHC) were evaluated at the quantitative trait locus (QTL) level. PH and PHC (including SL and internode lengths from the first to the fourth counted from the top, abbreviated as FIITL, SITL, TITL, and FOITL, respectively) were measured in four environments. Individual and the pooled values from four trials were used in the present analysis. A QTL for PH was mapped using data on PH and on PH conditioned by PHC using IciMapping V2.2. All 21 chromosomes in wheat were shown to harbor factors affecting PH in two populations, by both conditional and unconditional QTL mapping methods. At least 11 pairwise congruent QTL were identified in the two populations. In total, ten unconditional QTL and five conditional QTL that could be detected in the conditional analysis only have been verified in no less than three trials in WJ and WY. In addition, three QTL on the short arms of chromosomes 4B, 4D, and 7B were mapped to positions similar to those of the semi-dwarfing genes Rht-B1, Rht-D1 and Rht13, respectively. Conditional QTL mapping analysis in WJ and WY proved that, at the QTL level, SL contributed the least to PH, followed by FIITL; TITL had the strongest influence on PH, followed by SITL and FOITL. The results above indicated that the conditional QTL mapping method can be used to evaluate possible genetic relationships between PH and PHC, and it can efficiently and precisely reveal counteracting QTL, which will enhance the understanding of the genetic basis of PH in wheat. The combination of two related populations with a large/moderate population size made the results authentic and accurate.
Asunto(s)
Sitios de Carácter Cuantitativo/genética , Triticum/anatomía & histología , Triticum/genética , Mapeo Cromosómico , Cruzamientos Genéticos , Marcadores Genéticos/genética , Genotipo , FenotipoRESUMEN
Kernel dimensions (KD) contribute greatly to thousand-kernel weight (TKW) in wheat. In the present study, quantitative trait loci (QTL) for TKW, kernel length (KL), kernel width (KW) and kernel diameter ratio (KDR) were detected by both conditional and unconditional QTL mapping methods. Two related F(8:9) recombinant inbred line (RIL) populations, comprising 485 and 229 lines, respectively, were used in this study, and the trait phenotypes were evaluated in four environments. Unconditional QTL mapping analysis detected 77 additive QTL for four traits in two populations. Of these, 24 QTL were verified in at least three trials, and five of them were major QTL, thus being of great value for marker assisted selection in breeding programmes. Conditional QTL mapping analysis, compared with unconditional QTL mapping analysis, resulted in reduction in the number of QTL for TKW due to the elimination of TKW variations caused by its conditional traits; based on which we first dissected genetic control system involved in the synthetic process between TKW and KD at an individual QTL level. Results indicated that, at the QTL level, KW had the strongest influence on TKW, followed by KL, and KDR had the lowest level contribution to TKW. In addition, the present study proved that it is not all-inclusive to determine genetic relationships of a pairwise QTL for two related/causal traits based on whether they were co-located. Thus, conditional QTL mapping method should be used to evaluate possible genetic relationships of two related/causal traits.
Asunto(s)
Cromosomas de las Plantas/genética , Sitios de Carácter Cuantitativo , Semillas/crecimiento & desarrollo , Triticum/genética , Mapeo Cromosómico , Ambiente , Ligamiento Genético , Marcadores Genéticos , Genotipo , Endogamia , Fenotipo , Semillas/genética , Triticum/crecimiento & desarrolloRESUMEN
Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the major diseases of common wheat (Triticum aestivum) worldwide. The powdery mildew resistance gene Pm23, identified in the common wheat Line 81-7241 and originally assigned to wheat chromosome 5A, was relocated on chromosome 2AL with the aid of molecular markers. Mapping of microsatellite markers in two wheat crosses segregating for Pm23 and Pm4b, respectively, in combination with the reported mapping of Pm4a, indicated that the three genes were all linked to the marker Xgwm356 with a distance of 3-5 cM. Allelism between Pm4b and Pm23 was then confirmed, when the progenies of a cross between VPM1 (Pm4b) and Line 81-7241, were shown to be all resistant to a B. graminis isolate avirulent to the both parents. Pm23 is therefore a new allele of the Pm4 locus, and was redesignated as Pm4c.
Asunto(s)
Cromosomas de las Plantas , Genes de Plantas , Triticum/genética , Alelos , Mapeo Cromosómico , ADN de Plantas/genética , Repeticiones de Microsatélite , Enfermedades de las Plantas/genética , Triticum/microbiologíaRESUMEN
The transgenic wheat of improved resistance to the storage pest was production. We have introduced the cowpea trypsin inhibitor gene (CpTI) into cultured embryonic callus cells of immature embryos of wheat elite line by Agrobacterium-mediated method. Independent plantlets were obtained from the kanamycin-resistant calli after screening. PCR and real time PCR analysis, PCR-Southern and Southern blot hybridization indicated that there were 3 transgenic plants viz. transformed- I, II and III (T- I, T-II and T-III). The transformation frequencies were obviously affected by Agrobacterium concentration, the infection duration and transformation treatment. The segregations of CpTI in the transgenic wheat progenies were not easily to be elucidated, and some transgenic wheat lines (T- I and T-III) showed Mendelian segregations. The determinations of insect resistance to the stored grain insect of wheat viz. the grain moth (Sitotroga cerealella Olivier) indicated that the 3 transgenic wheat progeny seeds moth-resistance was improved significantly. The seed moth-eaten ratio of T- I, T-II, T-III and nontransformed control was 19.8%, 21.9%, 32.9% and 58.3% respectively. 3 transgenic wheat T1 PCR-positive plants revealed that the 3 transgenic lines had excellent agronomic traits. They supplied good germplasm resource of insect-resistance for wheat genetic improvement.
Asunto(s)
Proteínas de Plantas/genética , Triticum/genética , Inhibidores de Tripsina/genética , Agrobacterium tumefaciens/genética , Animales , Himenópteros/crecimiento & desarrollo , Lepidópteros/crecimiento & desarrollo , Pisum sativum/genética , Plantas Modificadas Genéticamente/genética , Transformación Genética , Inhibidores de Tripsina/biosíntesis , alfa-Amilasas/antagonistas & inhibidoresRESUMEN
To study the usefulness of low-molecular-weight glutenin subunits (LMW-GS) of Agropyron elongatum (Host) Nevski to wheat (Triticum aestivum L.) quality improvement, we characterized LMW-GS genes of A. elongatum. Nine LMW-GS genes of A. elongatum, which were named AeL1 to AeL9, were cloned by genomic PCR. After sequencing, we obtained complete open reading frames from AeL2 to AeL8 and partial genes of AeL1 and AeL9. All nine sequences are homoeologous to those of wheat and related grasses. Comparison of the deduced amino acid sequences with those of published LMW-GS suggests that the basic structures of all the subunits are very similar. However, except for AeL4 and AeL5, which contain the identical N-terminal sequence with LMW-m, other LMW-GS sequences separated from A. elongatum cannot be classified according to previous criteria for the three types: LMW-m (methionine), LMW-s (serine), and LMW-i (isoleucine), and then 12 groups. In addition, there are some characters in the LMW-GS sequences of A. elongatum: AeL2, AeL3, and AeL6 involve a Cys residue in the signal peptide respectively, which is absent in most of LMW-GS; AeL3, AeL6, AeL8, and AeL9 start their first Cys residues in the N-terminal repetitive domains, respectively; both AeL2 and AeL5 have nine Cys residues, with an extra Cys residue in the N-terminal repetitive domain and the repetitive and glutamine-rich domain; AeL2, AeL3, AeL6, and AeL9 comprise long repetitive domains. Phylogenetic analysis indicates that there is a relatively weak sequence identity between the LMW-GS genes from A. elongatum cloned in this study and those reported from other plants. Three LMW-GS sequences, AeL2, AeL3, and AeL6, are clustered to Glu-A3 from wheat than to those from other plants. The possible use of these genes in relation to the high quality of hybrid wheat is discussed.
Asunto(s)
Agropyron/genética , Cruzamiento/métodos , Glútenes/genética , Secuencia de Aminoácidos , Secuencia de Bases , Clonación Molecular , Análisis por Conglomerados , Cartilla de ADN , Datos de Secuencia Molecular , Análisis de Secuencia de ADN , Triticum/genéticaRESUMEN
High-molecular-weight glutenin subunits (HMW-GSs) from hybrid line II-12 between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nivski were characterized with SDS-PAGE. Out of these HMW-GSs, two subunits, h1Bx and h1By, had mobilities similar to the subunits 1Bx13 and 1By16 from common wheat 4072, which was used as control. Polyclonal antibodies (pAbs) of h1Bx and h1By were prepared, and Western blotting showed that the pAbs had strong affinities for h1Bx and h1By, separately. The specificity of h1Bx-pAb was further checked; it preferentially recognized subunits h1Bx and 1Bx13. HMW-GS gene coding sequences were amplified by genomic polymerase chain reaction from hybrid II-12. Two of the five amplicons, marked II2a and II31b, were sequenced. Their coding sequences are clustered to Glu-1Bx7 and Glu-1By9 of common wheat. Three discrepant regions in deduced amino acid sequences of II2a and 31b repeated one time more than Glu-1Bx7 and Glu-1By9. N-terminal sequences of h1Bx and h1By were determined, which were identical to the published sequences of 1Bx13 and 1By16 and in agreement with that deduced from II2a and II31b, respectively. These results indicated that the two novel genes separated from the hybrid wheat derived from the allelic variation of 1Bx7 and 1By9 of the parent wheat. There is an additional cysteine residue positioned at 271st amino acid of the mature peptide of II2a, which may be related to the high quality of the flour.