Revealing the interaction forms between Hg(II) and group types (-Cl, -CN, -NH2, -OH, -COOH) in functionalized Poly(pyrrole methane)s for efficient mercury removal.

To explore the impact of different functional groups on Hg(II) adsorption, a range of poly(pyrrole methane)s functionalized by -Cl, -CN, -NH2, -OH and -COOH were synthesized and applied to reveal the interaction between different functional groups and mercury ions in water, and the adsorption mechanism was revealed through combined FT-IR, XPS, and DFT calculations. The adsorption performance can be improved to varying degrees by the incorporation of functional groups. Among them, the oxygen-containing functional groups (-OH and -COOH) exhibit stronger affinity for Hg(II) and can increase the adsorption capacity from 180 mg g-1 to more than 1400 mg g-1 at 318 K, with distribution coefficient (Kd) exceeding 105 mL g-1. The variations in the capture and immobilization capabilities of functionalized poly(pyrrole methane)s predominantly stem from the unique interactions between their functional groups and mercury ions. In particular, oxygen-containing -OH and -COOH effectively capture Hg(OH)2 through hydrogen bonding, and further deprotonate to form the -O-Hg-OH and -COO-Hg-OH complexes which are more stable than those obtained from other functionalized groups. Finally, the ecological safety has been fully demonstrated through bactericidal and bacteriostatic experiments to prove the functionalized poly(pyrrole methane)s can be as an environmentally friendly adsorbent for purifying contaminated water.

Selective Adsorption Behavior of Sulfuric Acid Oxidized and Doped Conjugated Microporous Poly(aniline)s toward Lead Ions in an Aqueous Environment.

The coexistence of lead, zinc, and copper ions in wastewater constitutes an environmental challenge of pressing concern. This research delves into the preparation of innovative oxidation-doped conjugated microporous poly(aniline) frameworks, exploring their prospective efficacy in regulating lead ion adsorption from aqueous solutions. H2SO4-CMPTA demonstrates the capability to reach adsorption equilibrium within 15 min at a lead concentration of 50 ppm. Even at a lead concentration of 20 ppm, it still efficaciously attenuates these levels to sub-10 ppb, a value surpassing extant standard. H2SO4-CMPTA retains over 78.8% adsorption efficiency after six cycles. Analytical characterization coupled with computational calculations suggests that sulfate-coordinated nitrogen cationic structure plays a crucial role in adsorption. A deeper investigation reveals the cardinal role of electrostatic attraction and exclusive chelation adsorption underpinning the efficient capture of lead ions by doped sulfate ions. Intriguingly, in a mixed heavy metal solution containing lead, zinc, and copper ions, H2SO4-CMPTA exhibits an initial predilection toward zinc ions, yet an eventual ion-exchange adsorption gravitating toward lead ions was discerned, governed by the latter's superior binding energy. Our study elucidates a promising material as an efficacious tool for the remediation of aquatic environments tainted with lead contaminants.

Tuning Electromagnetic Parameters Induced by Synergistic Dual-Polarization Enhancement Mechanisms with Amorphous Cobalt Phosphide with Phosphorus Vacancies for Excellent Electromagnetic Wave Dissipation Performance.

The understanding of amorphous and heterojunction materials has been widely used in the field of electromagnetic wave absorption due to their unique physical and chemical properties. However, the effectiveness of individual strategies currently used is still limited. Well-designed compositions and amorphous structures simplify the effect of different polarization mechanisms on the absorption of electromagnetic waves. In this work, through the carbonization and controlled phosphating of one-dimensional Co Metal-Organic Framework (Co-MOF) nanorods, the synthesis of complex components and amorphous CoPx with phosphorus vacancies is successfully accomplished, thus adjusting the optimization of electromagnetic parameters. Phosphorus-vacancy-induced defective polarization loss and enhanced-electronegativity-differences-induced dipole polarization loss synergistically as a dual-polarization strategy significantly improved the electromagnetic parameters and impedance matching. In conclusion, the electromagnetic parameters of the Co@CoPx@C composites are indeed significantly regulated, with reflection losses of -55 dB and a bandwidth of up to 5.5 GHz. These innovative research ideas provide instructive thinking for the development of amorphous absorbers with vacancies.

Synthesis, characterization, antibacterial and antifungal activities of 1-O-alkylglycerols.

1-O-alkylglycerols are a class of natural existing lipids with broad biological activities. However, their use in food or agricultural fields remains to be investigated, especially for their antimicrobial activity. In this work, three 1-O-alkylglycerols, 1-O-octanylglycerol (C8Gly1), 1-O-dodecylglycerol (C12Gly1), and 1-O-hexadecylglycerol (C16Gly1), were synthesized in the isolated yields of 71.3-89.8 % and characterized by 1H NMR, 13C NMR, FITR, mass spectra, and HPLC-ESLD. The critical micelle concentration (CMC) of 1-O-alkylglycerols was determined to be 1.65 mmol/L (C8Gly1), 0.33 mmol/L (C12Gly1), and 0.23 mmol/L (C16Gly1) using the pyrene method. C12Gly1 and C16Gly1 had similar surface tensions that are lower than C8Gly1. C8Gly1 can form micelles in aqueous solution with excellent nano-dispersed uniformity and stability. Furthermore, C8Gly1 and C12Gly1 not only displayed good antibacterial activity against Staphylococcus aureus, but they also inhibited the growth of Botryosphaeria dothidea, Monilia fructigena, and Phytophthora capsicum at 400 µg/mL. Thus, the C8Gly1 and C12Gly1 can serve as novel antimicrobial agents in food preservation.

The in vivo and in vitro corrosion behavior of MgO/Mg-Zn-Ca composite with different Zn/Ca ratio.

The effect of Zn/Ca ratio on the corrosion behavior of Mg-3Zn-0.2Ca-1.0MgO (3ZX) and Mg-1Zn-0.2Ca-1.0MgO (ZX) was investigated on the as-extruded specimens. Microstructure observations revealed that the low Zn/Ca ratio led to the grain growth from 1.6 µm in 3ZX to 8.1 µm in ZX. At the same time, the low Zn/Ca ratio changed the nature of second phase from the existence of Mg-Zn and Ca2Mg6Zn3 phases in 3ZX to the dominated Ca2Mg6Zn3 phase in ZX. The local galvanic corrosion caused by the excessive potential difference was alleviated obviously due to the missing of MgZn phase in ZX. Besides, the in vivo experiment also showed that ZX composite exhibited a good corrosion performance and the bone tissue around the implant grew well.

Ideal vitamin D and handgrip strength counteracts the risk effect of APOE genotype on dementia: a population-based longitudinal study.

BACKGROUND: Higher vitamin D concentrations and grip strength contribute to lower individual-level risk of dementia, while apolipoprotein 4 (APOE e4) genotype carries increases dementia risk, but whether combination of ideal vitamin D and grip strength counteracts the risk effect of dementia related to APOE e4 genotype remains unclear. We aimed to investigate the interactions between vitamin D/grip strength and APOE e4 genotype and their association with dementia. METHODS: The UK Biobank cohort comprised 165,688 dementia-free participants (aged at least 60 years) for the dementia analysis. Dementia was ascertained using hospital inpatient, mortality, and self-reported data until 2021. Vitamin D and grip strength were collected at baseline and divided into tertiles. APOE genotype was coded as APOE e4 non-carries and APOE e4 carries. Data were analyzed using Cox proportional hazard models and restricted cubic regression splines, with adjusted for known confounders. RESULTS: Over the follow-up (median: 12.0 years), 3917 participants developed dementia. In women and men, respectively, compared with to the lowest tertile of vitamin D, the HRs (95% CIs) of dementia were lower in the middle [0.86 (0.76-0.97)/0.80 (0.72-0.90)] and the highest tertile [0.81 (0.72-0.90)/0.73 (0.66-0.81)]. Tertiles of grip strength showed similar patterns. In women and men, respectively, participants who had both highest tertile of vitamin D and grip strength was associated with a lower risk of dementia compared to those with both lowest tertile of these two exposures among APOE e4 genotype carries (HR = 0.56, 95% CI 0.42-0.76, and HR = 0.48, 95% CI 0.36-0.64) and APOE e4 genotype non-carries (HR = 0.56, 95% CI 0.38-0.81, and HR = 0.34, 95% CI 0.24-0.47). There were significant additive interactions between lower vitamin D/grip strength and APOE e4 genotype on dementia among women and men. CONCLUSIONS: Higher vitamin D and grip strength were associated with a lower risk of dementia, and seemed to halve the adverse effects of APOE e4 genotype on dementia. Our findings suggested that vitamin D and grip strength may be imperative for estimating the risks of dementia, especially among APOE e4 genotype carries.

Association of laxatives use with incident dementia and modifying effect of genetic susceptibility: a population-based cohort study with propensity score matching.

BACKGROUND: Constipation was associated with incidence of dementia and cognitive decline. Laxatives are the mainstay of constipation management and are commonly used among older populations for both treatment and prevention of constipation. However, the association between use of laxatives and incident dementia, and whether laxatives use may modify the effect of genetic predisposition on dementia remains unclear. METHODS: We applied 1:3 propensity score matching to balance the baseline characteristics of the laxative users versus non-users and to reduce potential confounders using multi-variates adjusted Cox hazards regression models. We categorized genetic risk into three groups (low, middle, and high) through a genetic risk score of common genetic variants. Information on laxatives use was assessed at baseline and categories into four varieties, including bulk forming laxatives, softeners and emollients, osmotic laxatives, and stimulant laxatives. RESULTS: Of 486,994 participants, there were 14,422 laxatives users in UK Biobank. After propensity score matching, participants with use of laxatives (n = 14,422) and matched non-laxative (n = 43,266) exposed individuals were enrolled. Over follow-up to 15 years, there were 1377 participants developed dementia (539 for Alzheimer's disease, and 343 for vascular dementia). The use of laxatives had greater risk of dementia (HR, 1.72; 95% CI:1.54-1.92), Alzheimer's disease (HR, 1.36; 95% CI: 1.13-1.63), and vascular dementia (HR, 1.53; 95% CI: 1.23-1.92). Compared to non-laxative exposed participants, those with use of softeners and emollients drugs, stimulant laxatives, and osmotic laxatives were associated with 96% (HR, 1.96; 95 CI: 1.23-3.12; P = 0.005), 80% (HR, 1.80; 95% CI: 1.37-2.37; P < 0.001), and 107% (HR, 2.07; 95% CI: 1.47-2.92; P < 0.001) higher risk of developed incident dementia, respectively. In joint effect analysis, compared to participants with low/middle genetic susceptibility and non-laxatives use, the HR (95% CIs) of dementia was 4.10 (3.49-4.81) for those with high genetic susceptibility plus use of laxatives. There was an additive interaction between laxatives use and genetic susceptibility on dementia (RERI: 0.736, 95% CI: 0.127 to 1.246; AP: 0.180, 95% CI: 0.047 to 0.312). CONCLUSIONS: Use of laxatives was associated with higher risk of dementia and modify the effect of genetic susceptibility on dementia. Our findings suggested that attention should be paid to the relationship between laxatives use and dementia, especially in people at high genetic susceptibility.

Evolution of Local Structural Motifs in Colloidal Quantum Dot Semiconductor Nanocrystals Leading to Nanofaceting.

Colloidal nanocrystals (NCs) have shown remarkable promise for optoelectronics, energy harvesting, photonics, and biomedical imaging. In addition to optimizing quantum confinement, the current challenge is to obtain a better understanding of the critical processing steps and their influence on the evolution of structural motifs. Computational simulations and electron microscopy presented in this work show that nanofaceting can occur during nanocrystal synthesis from a Pb-poor environment in a polar solvent. This could explain the curved interfaces and the olivelike-shaped NCs observed experimentally when these conditions are employed. Furthermore, the wettability of the PbS NCs solid film can be further modified via stoichiometry control, which impacts the interface band bending and, therefore, processes such as multiple junction deposition and interparticle epitaxial growth. Our results suggest that nanofaceting in NCs can become an inherent advantage when used to modulate band structures beyond what is traditionally possible in bulk crystals.

Selective mercury adsorption and enrichment enabled by phenylic carboxyl functionalized poly(pyrrole methane)s chelating polymers.

Mercury decontamination from water requires highly effective and efficient methods for maintaining public health and environmental protection. Herein, based on the coordination theory between functional groups and metal ions, we proposed phenylic carboxyl group-based poly(pyrrole methane)s (PPDCBAs) as highly efficient mercury removal materials for environmental remediation applications. It was found that PPDCBAs can efficiently adsorb and remove mercury(II) from aqueous solutions by functionalizing the molecular structure with phenylic carboxyl groups. Among the as-prepared PPDCBAs, poly[pyrrole-2, 5-diyl (4-carboxybenzylidane)] (PPD4CBA) with the carboxyl group at the para position can not only adsorb mercury over 1400 mg⋅g-1 but also achieve a 92.5 % mercury(II) uptake within 100 min by a very low dosage of 0.1 g⋅L-1. In addition, PPDCBAs exhibited excellent adsorption selectivity for mercury(II) compared with copper(II), cadmium(II), zinc(II) and lead(II). Furthermore, as determined by Fourier transform infrared (FT-IR) spectra, X-ray photoelectron spectroscopy (XPS) and the density functional theory (DFT) calculation, the mercury removal was found to be mainly dependent on the high density of chelating sites, the phenylic carboxyl moieties, which helped us to realize an ultra-trace amount mercury removal (from 10.8 µg⋅L-1 to 0.6-0.8 µg⋅L-1) for meeting drinking water standard requirements (1.0 µg⋅L-1).

Design, synthesis, high algicidal potency, and putative mode of action of new 2-cyclopropyl-4-aminopyrimidine hydrazones.

Control of cyanobacteria harmful algal blooms remains a global challenge. In the present study, a series of novel 2-cyclopropyl-4-aminopyrimidine hydrazones were designed and synthesized as potential algicides. Compounds 4a, 4b, 4h, 4j, 4k, 4l, and 4m showed potent inhibition against Synechocystis sp. PCC6803 (median effective concentration, EC50 = 1.1 to 1.7 µM) and Microcystis aeruginosa FACHB905 (EC50 = 1.2 to 2.0 µM), more potent than, or comparably with, copper sulfate (PCC6803, EC50 = 1.8 µM; FACHB905, EC50 = 2.2 µM) and prometryne (PCC6803, EC50 = 12.3 µM; FACHB905, EC50 = 7.2 µM). Compound 4k exhibited algicidal activity in an expanded culture system, and was less toxic than copper sulfate to zebrafish. Electron microscope analyses showed that 4k damaged cyanobacterial cells and decreased the number of thylakoid lamellae. Transcriptomic and qPCR analyses suggest that 4k interfered photosynthesis-related pathways. Treatment with 4k significantly decreased the maximum quantum yield of photosystem II and the photosynthetic electron transfer rate, and the resulting reactive oxygen species damaged thylakoid membranes and photosystem I. The results suggest that 4k is a potential lead for further development of effective and safe algicides.

Synergistic Fluoride Adsorption by Composite Adsorbents Synthesized From Different Types of Materials-A Review.

The reduction of fluoride concentrations in water is one of many concerns. Adsorption is the most widely used technology for fluoride removal and the center to development of adsorption technology is the improvement of adsorbents. This review classifies the typical fluoride removal adsorbents into four types: metal oxides/hydroxides, biopolymers, carbon-based, and other adsorbents. The exploitation of new materials and the synthesis of composite materials are two ways of developing new adsorbents. In comparison to the discovery of novel adsorbents for fluoride adsorption, research into the composite synthesis of different types of conventional adsorbents has proliferated in recent years. The traditional adsorbents used the earliest, metal oxides, can act as active centers in a wide range of applications for modifying and compounding with other types of adsorbents. This study emphasizes reviewing the research on fluoride removal by composite adsorbents synthesized from different types of metal-modified materials. Seven factors were compared in terms of material characterization, initial fluoride concentration, adsorbent dose, pH, temperature, reaction time, and maximum adsorption capacity. The modification of composite adsorbents is facile and the synergistic effect of the different types of adsorbents significantly improves fluoride adsorption capacity. Metal composite adsorbents are synthesized by facile coprecipitation, hydrothermal, or impregnation modification methods. The adsorption mechanisms involve electrostatic attraction, ion exchange, complexation, and hydrogen bonding. The fluoride adsorption capacity of composite adsorbents has generally improved, indicating that most modifications are successful and have application prospects. However, to achieve significant breakthroughs in practical applications, numerous issues such as cost, separation/regeneration performance, and safety still need to be considered.

Preparation of Templated Materials and Their Application to Typical Pollutants in Wastewater: A Review.

As the pollution and destruction of global water resources become more and more severe, the treatment of wastewater has attracted significant attention. The template method is a synthetic method in which the template is the main configuration to control, influence, and modify the morphology as well as control the dimensions of the material, thus achieving the properties that determine the material. It is simple, highly reproducible, and predictable, and more importantly, it can effectively control the pore structure, size, and morphology of the material, providing a novel platform for the preparation of adsorbent materials with excellent adsorption properties. This review focuses on the classification of the templates according to their properties and spatial domain-limiting capabilities, reviews the types of hard and soft template materials and their synthetic routes, and further discusses the modulation of the morphological structure of the materials by the introduction of templates. In addition, the application and adsorption mechanisms of heavy metal ions and dyes are reviewed based on the regulatory behavior of the template method.

Insight into the ion exchange in the adsorptive removal of fluoride by doped polypyrrole from water.

In this study, the polypyrrole (PPy) samples doped with Cl- (PPy-Cl), SO42- (PPy-SO4) and SO42-+Cl- (PPy-SO4+Cl) were synthesized by chemical polymerization for the adsorptive removal of fluoride ion from water. The structure and morphology of the as-prepared PPy samples were characterized by FT-IR, BET, SEM, XPS, and zeta potential. The adsorption experiments revealed that the PPy-Cl exhibited faster kinetics and higher adsorption capacity (13.98 mg/g), more than 4 times that of PPy-SO4 (3.08 mg/g) and PPy-SO4+Cl (3.17 mg/g). The kinetics of the adsorption followed the pseudo-second-order model and the adsorption isotherm data fitted well to the Langmuir model. FT-IR, EDX, and XPS tests for PPy samples before and after fluoride adsorption demonstrated that anion exchange between F- and Cl- or SO42- was the prior mechanism for fluoride ion removal from water. Cl- was more favorable than SO42- in the ion exchange with F-. Meanwhile, the Cl- or SO42- exchanged with F- was mainly bound to the active nitrogen that accounts for 6% of the total nitrogen in PPy molecular matrix. Further study of zeta potential and pH influence experiment demonstrated the electrostatic interaction is auxiliary interaction for the fluoride removal by doped PPy samples.

Design, Synthesis, and Antifungal Activity of 2,6-Dimethyl-4-aminopyrimidine Hydrazones as PDHc-E1 Inhibitors with a Novel Binding Mode.

A series of novel 2,6-dimethyl-4-aminopyrimidine hydrazones 5 were rationally designed and synthesized as pyruvate dehydrogenase complex E1 (PDHc-E1) inhibitors. Compounds 5 strongly inhibited Escherichia coli (E. coli) PDHc-E1 (IC50 values 0.94-15.80 µM). As revealed by molecular docking, site-directed mutagenesis, enzymatic, and inhibition kinetic analyses, compounds 5 competitively inhibited PDHc-E1 and bound in a "straight" pattern at the E. coli PDHc-E1 active site, which is a new binding mode. In in vitro antifungal assays, most compounds 5 at 50 µg/mL showed more than 80% inhibition against the mycelial growth of six tested phytopathogenic fungi, including Botrytis cinerea, Monilia fructigena, Colletotrichum gloeosporioides, andBotryosphaeria dothidea. Notably, 5f and 5i were 1.8-380 fold more potent against M. fructigena than the commercial fungicides captan and chlorothalonil. In vivo, 5f and 5i controlled the growth of M. fructigena comparably to the commercial fungicide tebuconazole. Thus, 5f and 5i have potential commercial value for the control of peach brown rot caused by M. fructigena.

Effective removal of ammonium nitrogen using titanate adsorbent: Capacity evaluation focusing on cation exchange.

Cation exchange is one of the dominant mechanisms in the adsorption of cationic ammonium nitrogen (NH4+) from water. In this study, we focus on the role of counter cations in cation exchange process of NH4+ to enhance the adsorption capacity. Five amorphous titanates namely lithium titanate (LiT), sodium titanate (NaT), potassium titanate (KT), strontium titanate (SrT) and barium titanate (BaT) with different counter cation were facilely synthesized. The adsorption performance for NH4+ by these samples is in the order of LiT> NaT > KT> > SrT > BaT. The maximum adsorption capacity of LiT calculated by Langmuir is as high as 50.31 mg·g-1. According to the experimental results and theoretical analysis, the electrostatic interaction between counter ions (cations in framework or eternal solution) and charged framework (fixed ions) is the main influence factor during cation exchange process in general. The cation valence and the hydrated ionic radius of the counter ions can inversely affect the ion exchange equilibrium and the affinity of counter ions to titanates. Therefore, a definition of a brief parameter, affinity coefficient Kf (relating to ion valence and distance between opposite charged ions), is introduced and used to explain the difference in adsorption performance of five titanates for NH4+. The conclusion about cation exchange and ions affinity may provide possible strategies for enhancement of cationic contaminant adsorption from water or wastewater.

Insight into the effect of surfactant modification on the versatile adsorption of titanate-based materials for cationic and anionic contaminants.

The new challenges to adsorption are imposed for the diversity of contaminants in wastewater in recent years. Herein, titanate-based materials (peroxide sodium titanate, PST) were modified by three different kinds of surface charged surfactant: dodecyl dimethyl betaine (BS-PST), sodium dodecyl sulphate (SDS-PST) and dodecyltrimethyl ammonium chloride (DTAC-PST) to enhance the versatile adsorption performance for four typical contaminants including ammonia nitrogen (NH4+, inorganic and cationic), phosphate (H2PO4-, inorganic and anionic), methylene blue (MB, organic and cationic) and Acid Red G (ARG, organic and anionic). The batch adsorption experiments showed that the DTAC-PST exhibited better in the removal of MB, ARG and H2PO4- than that of other adsorbents. The theoretical maximum adsorption capacity of DTAC-PST is 49.28 mg g-1 for NH4+, 34.74 mg g-1 for TP, 81.87 mg g-1 for MB and 545.81 mg g-1 for ARG. The simultaneous adsorption results showed that the concentration (10 mg L-1 of NH4+, 3 mg L-1 of TP, 50 mg L-1 of MB and 50 mg L-1 of ARG) of all the four chemicals in simulated wastewater could be controlled to be below the discharge levels in China (GB, 18918-2002) by DTAC-PST at the pH of 3.0. The FT-IR spectra demonstrated that ion exchange was the main way for NH4+ removal, however, electrostatic attraction and ligand exchange were the reason for MB adsorption. In addition, C-N+ from DTAC modification made main contribution to the excellent adsorption performance for ARG and H2PO4-. The saturated DTAC-PST could be conveniently regenerated by 0.5 mol L-1 NaOH solution and maintained about 80% of adsorption capacity after five cycles.

Does Revision Anterior Cruciate Ligament (ACL) Reconstruction Provide Similar Clinical Outcomes to Primary ACL Reconstruction? A Systematic Review and Meta-Analysis.

More revisionary reconstruction procedures are required following failing anterior cruciate ligament (ACL) reconstructions, which are often regarded as a technique challenge with very limited goals. This study will be performed to compare the outcomes between groups of primary and revision knee reconstruction. Two observers conducted the literature retrieval from the platforms of PubMed, Embase, and CENTRAL. Studies which compared knee function and stability between primary and revisionary reconstructions were included. The data was synthesized by meta-analysis with fixed- or random-effects models as appropriate. A total of 10 eligible studies were included with 954 subjects in the primary group and 378 in the revision group. The International Knee Documentation Committee International Knee Documentation Committee (IKDC) subscores, side-to-side difference, and Lysholm score were demonstrated to be significantly improved at final follow-up in both groups, while Tegner score was not. The overall IKDC, Knee injury and Osteoarthritis Outcome Score (KOOS), and Lysholm scores were significantly inferior in the revision group compared to the primary group. However, knee laxity according to side-to-side difference was demonstrated to be similar between the two groups. Revision ACL reconstruction (RACLR) could provide patients with excellent restoration of knee outcomes compared to the status before revision. Also, while knee function in the revision group was inferior to the primary group, knee stability was equivalent between the two groups at the final follow-up.

Clinical Outcomes of the Central Third Patellar Tendon Versus Four-strand Hamstring Tendon Autograft Used for Anterior Cruciate Ligament Reconstruction: A Systematic Review and Subgroup Meta-analysis of Randomized Controlled Trials.

BACKGROUND: The most frequently used auto-grafts for ACL reconstruction (ACLR) are bone-patellar tendon-bone (BPTB) and four-strand hamstring tendon (4S-HT), but significant controversy exists about which graft is better. This study aimed to compare the knee outcomes following ACLR with BPTB and 4S-HT autografts. METHODS: Subgroup meta-analyses were performed basing on available randomised controlled trials (RCTs) according to different follow-up intervals. 23 RCTs were eligible for inclusion, involving 933 participants in each treatment group. RESULTS: Only short-term (≤2 years) knee anteroposterior and rotational stability were significantly poorer with 4S-HT when compared to BPTB autograft, as judged by side-to-side difference on knee laxity (WMD=-0.50, 95%CI -0.81~-0.18, p=0.002) and pivot-shift test (OR=0.58, 95%CI 0.39~0.88, p=0.010), respectively. The IKDC objective grade was evident to be in favour of 4S-HT group in mid-term (3-5 years) follow-up (OR=0.63, 95%CI 0.43~0.92, p=0.017). Several donor-site related complications, including anterior knee pain, pain with kneeling and extension deficit, were more frequent in BPTB autograft group, while lower short-term iskinetic peak flexion torques both at 60°/S (WMD=8.93, 95%CI 7.88~9.99, p<0.001) and 240°/S (WMD=11.63, 95%CI 9.92~13.34, p<0.001) were caused by HT harvesting. CONCLUSION: BPTB was associated with inferior mid- to long-term (>5 years) knee functional outcomes, and increased frequency of donor-site complications. In contrast, only short-term knee laxity was increased for 4S-HT autograft, which is expected to be improved by providing more conservative rehabilitation protocol and aperture fixation methods.

Visceral Metastasis: A Prognostic Factor of Survival in Patients with Spinal Metastases.

OBJECTIVE: To characterize the visceral metastasis as a predictive tool for the survival of patients with spinal metastases through an exploratory meta-analysis. METHODS: Two investigators independently searched PubMed and Embase databases for eligible studies from 2000-2016. The effect estimates for the hazard ratio (HR) or risk ratio (RR) and 95% confidence interval (CI) were collected and pooled with a random- or fixed-effect model. RESULTS: In total, 18 eligible studies were retrieved with 5468 participants from nine countries. The overall pooled effect size for HR and RR was 1.50 and 3.79, respectively, which was proved to be statistically significant. In the subgroup of prostate cancer (PCa) and non-small cell lung cancer (NSCLC), statistical significance and marginal statistical significance was presented for the pooled HR (HR = 1.76, 95% CI 1.35-2.29) and (RR = 1.56, 95% CI 0.99-2.48), respectively. However, in the subgroup of thyroid cancer, breast cancer, and renal cancer, statistical significance was not achieved (HR = 1.17, 95% CI 0.75-1.83, Z = 0.70, P = 0.486). The results did not show any evidence of publication bias. CONCLUSIONS: This study demonstrated that visceral metastasis was a significant prognostic factor in patients with spinal metastases as a whole. Interestingly, the onset of visceral metastases differentially impacted the survival in different primary tumors. Therefore, the prognostic value of visceral metastasis might be related to the type of primary tumor.

Assessment of the Defatting Efficacy of Mechanical and Chemical Treatment for Allograft Cancellous Bone and Its Effects on Biomechanics Properties of Bone.

OBJECTIVE: To assess the defatting efficacy of high pressure washing and gradient alcohol and biomechanical properties of defatted bone. METHODS: Fresh cancellous bone was obtained from the femoral condyle and divided into six groups according to different defatting treatments, which were: high pressure washing for 10 s (10S group), 20 s (20S group), and 30 s (30S group), gradient alcohol immersion (Alcohol group), acetone immersion (Acetone group), and non-defatted (Fresh group). The appearance of six groups was observed, and the appearance of defatted bone and fresh bone was compared. The residual lipid content and infrared spectrum were used to compare the efficacy of defatting, the DNA content was used to compare the cell content after defatting, and the maximum stress and elastic modulus were used to compare the effects of defatting treatment on biomechanical properties. RESULTS: The fresh bone was yellow and the pores contained a lot of fat. The defatted bone was white and the porous network was clear. There was no difference in residual lipid content among the three groups with high pressure washing (1.45% ± 0.16%, 1.40% ± 0.13%, and 1.46% ± 0.11%, respectively) (P = 0.828). There was no difference in residual lipid content among the 10S, alcohol, and acetone groups (1.45% ± 0.16%, 1.28% ± 0.07%, and 1.13% ± 0.22%, respectively) (P = 0.125). Infrared spectra showed that the fat content of the five defatting groups was significantly lower than that of the fresh group. There was no difference in residual lipid content among the three groups with high pressure washing (4.53 ± 0.23 ug/mL, 4.61 ± 0.18 ug/mL, and 4.66 ± 0.25 ug/mL, respectively) (P = 0.645). There was no difference in residual lipid content among the 10S, alcohol, and acetone groups (4.53 ± 0.23 ug/mL, 4.29 ± 0.24 ug/mL, and 4.27 ± 0.29 ug/mL, respectively) (P = 0.247). The maximum stress of the bone decreased significantly with the increase of the washing time (9.95 ± 0.31 Mpa, 9.07 ± 0.45 Mpa, and 8.17 ± 0.35 Mpa, respectively) (P = 0.003). The elastic modulus of the bone decreased significantly with the increase of the washing time (116.40 ± 3.54 Mpa, 106.10 ± 5.29 Mpa, and 95.63 ± 4.08 Mpa, respectively) (P = 0.003). There was no statistical difference in the maximum stress between the fresh group, the 10S group, the alcohol group, and the acetone group (10.09 ± 0.67 Mpa, 9.95 ± 0.31 Mpa, 10.11 ± 0.07 Mpa, and 10.09 ± 0.39 Mpa, respectively) (P = 0.963). There was no statistical difference in the maximum stress between the fresh group, the 10S group, the alcohol group and the acetone group (119.93 ± 4.94 Mpa, 116.40 ± 3.54 Mpa, 118.27 ± 0.85 Mpa, 118.10 ± 4.52 Mpa, respectively) (P = 0.737). CONCLUSION: The defatting efficiency was satisfactory at a time of 10 s under high pressure washing. In terms of defatting efficiency and its effect on biomechanical properties of bone, high pressure washing and gradient alcohol were similar to conventional acetone solvent extraction defatting.