[Changes of brain CT imaging of cerebral cysticercosis patients during the period of anti-cysticercus chemotherapy].

OBJECTIVE: To investigate the changes of brain CT imaging in patients with cerebral cysticercosis during the period of anti-cysticercus chemotherapy. METHODS: A total of 380 inpatients that were conformed to the practical diagnostic criteria of cerebral cysticercosis were enrolled in this study in the Third Affiliated Hospital of Shandong Academy of Medical Sciences from May 2010 to May 2015. All the patients were given anti-cysticercus chemotherapy (albendazole and praziquantel). All the patients received brain CT scan, and of which 210 received enhanced scan. The data of CT imaging were systematically reviewed. RESULTS: Before the treatment, the CT images of the patients showed single or multiple small cystic (s) with low density and small nodule-like cephalomere with high density. The re-examinations of CT showed that there were 81.58% (310/380) of the patients whose low density foci were completely absorbed, there were 16.32% (62/380) of the patients whose foci were mostly absorbed, and there were 2.11% (8/380) of the patients whose foci were calcified. Along with the prolongation of treatment time, the side effects were gradually reduced, and in the third course of treatment, the foci were absorbed or calcified in most of the patients. CONCLUSIONS: CT examination can diagnose the lesion site, range and classification of cerebral cysticercosis, and can evaluate the effect of the therapy according to the changes of CT imaging during the period of anti-cysticercus chemotherapy.

Changes of brain CT imaging of cerebral cysticercosis patients during the period of anti-cysticercus chemotherapy / 中国血吸虫病防治杂志

Objective To investigate the changes of brain CT imaging in patients with cerebral cysticercosis during the period of anti-cysticercus chemotherapy. Methods A total of 380 inpatients that were conformed to the practical diagnostic criteria of cerebral cysticercosis were enrolled in this study in the Third Affiliated Hospital of Shandong Academy of Medical Sciences from May 2010 to May 2015. All the patients were given anti-cysticercus chemotherapy (albendazole and praziquantel). All the patients received brain CT scan, and of which 210 received enhanced scan. The data of CT imaging were systematically reviewed. Results Before the treatment, the CT images of the patients showed single or multiple small cystic (s) with low density and small nodule-like cephalomere with high density. The re-examinations of CT showed that there were 81.58% (310/380) of the patients whose low density foci were completely absorbed, there were 16.32% (62/380) of the patients whose foci were mostly absorbed, and there were 2.11% (8/380) of the patients whose foci were calcified. Along with the prolongation of treatment time, the side effects were gradually reduced, and in the third course of treatment, the foci were absorbed or calcified in most of the patients. Conclusion CT examination can diagnose the lesion site, range and classification of cerebral cysticercosis, and can evaluate the effect of the therapy according to the changes of CT imaging during the period of anti-cysticercus chemotherapy.

Changes of brain CT imaging of cerebral cysticercosis patients during the period of anti-cysticercus chemotherapy / 中国血吸虫病防治杂志

Objective To investigate the changes of brain CT imaging in patients with cerebral cysticercosis during the period of anti-cysticercus chemotherapy. Methods A total of 380 inpatients that were conformed to the practical diagnostic criteria of cerebral cysticercosis were enrolled in this study in the Third Affiliated Hospital of Shandong Academy of Medical Sciences from May 2010 to May 2015. All the patients were given anti-cysticercus chemotherapy (albendazole and praziquantel). All the patients received brain CT scan, and of which 210 received enhanced scan. The data of CT imaging were systematically reviewed. Results Before the treatment, the CT images of the patients showed single or multiple small cystic (s) with low density and small nodule-like cephalomere with high density. The re-examinations of CT showed that there were 81.58% (310/380) of the patients whose low density foci were completely absorbed, there were 16.32% (62/380) of the patients whose foci were mostly absorbed, and there were 2.11% (8/380) of the patients whose foci were calcified. Along with the prolongation of treatment time, the side effects were gradually reduced, and in the third course of treatment, the foci were absorbed or calcified in most of the patients. Conclusion CT examination can diagnose the lesion site, range and classification of cerebral cysticercosis, and can evaluate the effect of the therapy according to the changes of CT imaging during the period of anti-cysticercus chemotherapy.

[Allele genetypes and homology analysis of MSP-1 and CSP gene of Plasmodium vivax in Shandong Province].

OBJECTIVE: To analyze the genotypes and homology of MSP-1 and CSP gene of Plasmodium vivax in Shandong Province, so as to provide the evidence for case traceability. METHODS: A total of 12 blood samples were collected from P. vivax-infected cases in Shandong Province in 2011. Parasite genomic DNA was extracted. Primers were designed according to MSP-1 and CSP gene sequences of P. vivax. Then Nested PCR, enzyme digestion, sequencing and sequence alignment, and homologous analysis were performed. RESULTS: The MSP-1 gene of all the 12 samples from P. vivax-infected cases were detected with a 470 bp PCR amplification band, and 350 bp and 120 bp enzyme digestion fragments, which were identified as type Sal-1. An analysis of phylogenetic tree of MSP-1 gene showed that the sequences of 9 indigenous case samples in Shandong Province were located in the same branch, one case sample infected from India was located in the same branch with India strains. All the 12 P. vivax-infected samples covered GDRA (D/A) GQPA sequences in CSP gene, which were identified as type PV-Ⅰ. Of the CSP gene among 12 P. vivax-infected samples, 10 samples of indigenous case in Shandong Province and one sample of the case infected in Guangdong Province were detected with both 560-840 bp and 150-230 bp PCR amplification bands, which were identified as temperate zone family strain of type PV-Ⅰ. However, one sample from the case infected in India was detected only with a 560-840 bp band, which was identified as tropical zone family strain of PV-Ⅰ. An analysis of phylogenetic tree of CSP gene showed that the sequences of 10 samples from the indigenous cases in Shandong Province and one sample from the case infected in Guangdong Province were located in the same branch, one sample from the case infected in India was located in the same branch with India and Indonesia strains. CONCLUSIONS: Of all the indigenous isolates in Shandong Province, MSP-1 gene is genotyped type Sal-1, CSP gene is genotyped temperate zone family strain of type PV-Ⅰ, with a high homology found among the indigenous isolates.