Graphene oxide-based nanomaterial interaction with human breast cancer cells.

Graphene and graphene-based nanomaterials have great potential for various biomedical applications due to their unique physicochemical properties. However, how graphene-based nanomaterials interact with biological systems has not been thoroughly studied. This study shows that 24, 48, and 72 hr exposure of 2.4 µg/cm2 of graphene oxide (GOX) and GOX modified with DAB-AM-16 and PAMAM dendrimers (GOXD and GOXP, respectively) did not exhibit toxicity to MCF-7 cells. However, higher graphene concentrations, such as 24 and 48 µg/cm2 , induced low cytotoxic effects. The GOX, GOXD, and GOXP particles have a strong affinity with the cellular membrane. Cells that internalized the nanomaterials presented morphological alterations and modifications in the organization of microfilaments and microtubules compared with control cells. Then, cells were treated with 24 µg/cm2 of GOX, GOXD or GOXP for 24 hr and recovered for an additional period of 24 hr in normal medium. Nanoparticles remained in the cytoplasm of some cells, apparently with no effect on cellular morphology, being consistent with the data found in the cell proliferation experiment, which showed that the cells remained alive up to 72 hr.

Motivo do atendimento odontológico na primeira infância / Reason for odontological appointment in the fi rst childhood

Preocupado com a falta de conscientização dos pais com relação à saúde bucal de seus filhos, este trabalho avaliou, por meio de uma revisão em 80 prontuários de atendimento, o motivo da consulta odontológica de crianças de zero a três anos de idade na Clínica de Bebês da ULBRA/Torres. Os dados coletados demonstraram que o motivo da consulta foi por prevençãoem 52,3% dos casos, 32,5% por cárie dentária, 11,2% por traumatismo e somente 5% das crianças procuraram atendimento por afecções. Conclui-se que, embora já exista algum conhecimento com relação ao atendimento do bebê, o Cirurgião-Dentista deve desempenhar papel ativo de educador e, junto com o pediatra, abordar a criança precocemente, levando mais informações para a conscientização e manutenção da saúde bucal das crianças.

Concerned with the parent´s awareness related to the children´s mouth health, this work evaluated by the means of 80 charts reviewing. The reason for appointment children from 0 to 3years old on Baby Clinic ULBRA Torres Campus. The data collected show that in 52,3% of the cases the reason of appointment was prevention, 32,5% was caries, 11,2% was traumatism and only 5% of patients searched for treatment due to affections. It was concluded that, although there isalready some knowledge related to baby’s appointments, the Surgeon-Dentist must execute activerole of educator and together with the pediatrician, approach the children precociously, bringing more information to the population´s awareness and maintenance of children´s mouth health.