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1.
Children (Basel) ; 11(5)2024 Apr 30.
Artículo en Inglés | MEDLINE | ID: mdl-38790530

RESUMEN

The scientific literature supports that practicing positive coparenting leads to the healthy development of children. Consequently, professional interest in parenting and coparenting has experienced significant growth, and evaluating coparenting is crucial in family psychology for establishing action protocols in clinical practice. An instrument highly regarded within the scientific community for evaluating coparenting dynamics is The Coparenting Relationship Scale (CRS). This research aims to achieve two objectives: first, to adapt the CRS for the Spanish population of both engaged and separated/divorced parents and to ascertain its reliability, validity, and factorial invariance psychometric properties; second, to assess the effectiveness of the total coparenting measure in categorizing sample participants. A cross-sectional non-experimental investigation was conducted to address these objectives. The first objective was answered by conducting an instrumental study, and the second by an exploratory study using classification techniques and a causal-comparative study using multivariate inferential methods. It was concluded that the model comprising 20 items across two factors, Positive Coparenting and Negative Perception of Coparenting, is the simplest and best fit for the Spanish parent sample; it is invariant regarding gender and marital status, and the measures derived from each factor demonstrate reliability and convergent and discriminant validity. The resulting questionnaire for Spanish parents is named CRS-SEg-S&D. The Coparental Vitality measure calculated using the total weighted measure of CRS-SEg-S&D allows the sample of participants to be divided into three differentiated clusters called Coparental Robustness, Moderate Coparenting, and Coparenting Rickets.

2.
Brain Spine ; 4: 102798, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38596449

RESUMEN

Background: Patients with multiple sclerosis (MS) are more likely to develop trigeminal neuralgia (TN) compared to the regular population, due to scarring of the nerve and development of a demyelination plaque. Despite treatment, approximately 10% of MS patients treated for TN experience symptom recurrence, including the development of MS-like symptoms such as optic neuritis and bilateral facial pain. Methods: A computed tomography (CT) scan was performed preoperatively on two patients diagnosed with multiple sclerosis (MS) who experienced secondary trigeminal neuralgia (TN). A precise reference frame was strapped firmly to the patient's forehead during the intraoperative procedure. Preliminary CT images were registered using the navigation system and the bony landmarks were set. Case description: Two patients diagnosed with multiple sclerosis (MS) who experienced refractory trigeminal neuralgia (TN) underwent percutaneous balloon compression. Initial conservative treatment and one dosage of Gamma Knife Radiosurgery (GKR) resulted in symptom control for a few weeks. Both patients had an acute recurrence of pain; thus, percutaneous retrogasserian balloon compression was performed. During follow-up, the patients reported a 70% decrease in pain after the procedure, with minimal recurrence of shooting episodes. Conclusion: Management of trigeminal neuralgia secondary to drug-resistant multiple sclerosis presents a persistent challenge. The percutaneous technique for retrogasserian balloon compression may offer a solution for some patients, but it presents unique challenges for neurosurgeons. Given the complexity of the pathogenesis, target identification, and the potential absence of neurovascular conflict, microvascular decompression remains a debated approach for this patient population. While stereotactic radiosurgery may be a promising alternative.

3.
Behav Sci (Basel) ; 13(12)2023 Nov 27.
Artículo en Inglés | MEDLINE | ID: mdl-38131831

RESUMEN

Difficulty in performing instrumental activities of daily living (IADLs) is currently considered an important indicator of cognitive impairment in the elderly. A non-experimental case-control investigation was conducted to assess the convergent validity of the PA-IADL with traditional (standard) cognitive assessment tests in its ability to identify adults with mild cognitive impairment. The analysis of the data was carried out by means of various multivariate statistical tests, and the sequence in its execution led to the conclusion that 8 of the 12 Tasks that make up the PA-IADL allow for the identification of people with mild cognitive impairment (MCI) to the same extent as traditional cognitive assessment tests and regardless of age. Age was found to be a moderating variable in the performance of the eight tasks; however, the results allow us to hypothesize that people with MCI experience a significant decline when it happens but thereafter, the deterioration that occurs does so at the same rate as the deterioration experienced by healthy people. They also allow us to hypothesize that the difference in the cognitive skills required by the eight functional tasks, and therefore also in the cognitive skills required by the traditional (standard) tests of a person with MCI compared to a person of the same age without MCI (Healthy), is approximately 10 years. These hypotheses have remarkable relevance and should be tested via longitudinal research. In the meantime, the results highlight the importance of the IADL assessment for the diagnosis of MCI as a complement to the standard cognitive assessment.

5.
Psychol Methods ; 2023 Feb 16.
Artículo en Inglés | MEDLINE | ID: mdl-36795436

RESUMEN

This article discusses the robustness of the multivariate analysis of covariance (MANCOVA) test for an emergent variable system and proposes a modification of this test to obtain adequate information from heterogeneous normal observations. The proposed approach for testing potential effects in heterogeneous MANCOVA models can be adopted effectively, regardless of the degree of heterogeneity and sample size imbalance. As our method was not designed to handle missing values, we also show how to derive the formulas for pooling the results of multiple-imputation-based analyses into a single final estimate. Results of simulated studies and analysis of real-data show that the proposed combining rules provide adequate coverage and power. Based on the current evidence, the two solutions suggested could be effectively used by researchers for testing hypotheses, provided that the data conform to normality. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

6.
Cancers (Basel) ; 14(14)2022 Jul 09.
Artículo en Inglés | MEDLINE | ID: mdl-35884408

RESUMEN

BACKGROUND: Laryngectomized patients have communicative limitations when they lose their phonatory function after surgery. The scale "Self-Evaluation of Communication Experiences after Laryngectomy" (SECEL) assesses the impact of total laryngectomy on quality of life related to communication. The aim of this study was to translate and adapt the SECEL from English to Spanish and to apply this first version to a group of patients to check its reliability and validity. MATERIALS AND METHODS: One-hundred-and-one laryngectomized patients completed the SECEL scale adjusted to Spanish, the Voice Handicap Index (VHI-30), the Hospital Anxiety and Depression Scale (HADS) and the EORTC QLQ-H&N35 questionnaire. RESULTS: The Spanish version of the SECEL questionnaire has the same format and conceptual, semantic and idiomatic meaning as the original. The resulting model is composed of 21 items dimensioned in two highly correlated factors that are clear, meaningful, and replicable leading to measures that are reliable enough to be used in clinical evaluation. CONCLUSION: The Spanish translation and validation of the SECEL questionnaire were performed. It can be considered an appropriate tool to assess communication skills from laryngectomized people.

7.
Front Psychol ; 13: 889201, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35645884

RESUMEN

An experimental study was designed to analyze the effect of school-based training in self-regulation learning strategies on academic performance (Mathematics, Sciences, Language, and English). Class-level variables (i.e., gender, the teacher's teaching experience, class size) were considered and the effects of the intervention were measured at the end of the intervention and 3 months later. A sample of 761 students from 3rd and 4th grades (356 in the control condition and 405 in the experimental condition), from 14 schools, participated in the study. Data were analyzed using three-level analysis with within-student measurements at level 1, between-students within-classes at level 2, and between-classes at level 3. Data showed a positive effect of the intervention on student performance, both at post-test (d = 0.25) and at follow-up (d = 0.33) considering the four school subjects together. However, the effect was significant just at follow-up when subjects were considered separately. Student performance was significantly related to the students' variables (i.e., gender, level of reading comprehension) and the context (teacher gender and class size). Finally, students' gender and level of reading comprehension, as well as the teacher's gender, were found to moderate the effect of the intervention on students' academic performance. Two conclusions were highlighted: first, data emphasize the importance of considering time while conducting intervention studies. Second, more teaching experience does not necessarily translate into improvements in the quality of students' instruction.

8.
Case reports (Universidad Nacional de Colombia. En línea) ; 8(1): 41-50, Jan.-June 2022. tab, graf
Artículo en Inglés | LILACS-Express | LILACS | ID: biblio-1421081

RESUMEN

ABSTRACT Introduction: Tufting enteropathy is a rare cause of congenital diarrhea in neonates. It is characterized by the abnormal distribution of epithelial adhesion molecules, which causes enterocytes to shed into the lumen, forming the characteristic tufts. Case summary: A 15-day-old female neonate was taken by her parents to the emergency department of a tertiary care hospital due to diarrheal stools she had been experiencing since birth. The patient presented with dehydration, abnormal weight loss, metabolic acidosis, and acute kidney failure. She received treatment with alizapride, loperamide, zinc sulfate, and probiotics, but after 75 days of treatment she was still symptomatic. An upper tract endoscopy and colonoscopy were performed, finding flattening of the villi and lymphoid cells in the lamina propria. However, the symptoms persisted, and she died at the age of ten months. A post-mortem exome sequencing reported tufting enteropathy. Conclusions. When congenital diarrhea is present, tufting enteropathy should be considered. An early molecular study would allow to evaluate the possibility of performing an intestinal transplant or modifying the treatment to meet the patient's palliative care needs.


RESUMEN Introducción. La enteropatía en penacho es una causa rara de diarrea congénita en neonatos; esta se caracteriza por una alteración de la adhesión epitelial que ocasiona desprendimiento de enterocitos hacia el lumen y, en consecuencia, forma los característicos penachos. Se describe el caso de una paciente con esta patología. Presentación del caso. Neonata de 15 días de vida, quien fue llevada por sus padres al servicio de urgencias de un hospital de tercer nivel debido a que desde su nacimiento tuvo deposiciones diarreicas y a causa de esto presentó deshidratación, pérdida de peso, acidosis metabólica e insuficiencia renal aguda. La paciente recibió manejo con alizaprida, loperamida, sulfato de zinc y probióticos, pero a los 75 días de tratamiento continuaba sintomática. Se le practicó una endoscopia de vías digestivas y una colonoscopia que mostraron aplanamiento de las vellosidades e infiltrado de células linfoides en la lámina propia. Los síntomas continuaron y la menor falleció a los 10 meses de nacida. El resultado del exoma post mortem reportó enteropatía en penacho. Conclusiones. Ante la presencia de diarrea congénita, se debe sospechar de una enteropatía en penacho y considerar el estudio molecular temprano, pues este permite evaluar la posibilidad de realizar un trasplante intestinal o modificar el tratamiento según las necesidades de cuidado paliativo del paciente.

9.
Food Chem ; 371: 131199, 2022 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-34598122

RESUMEN

Antiglycation activities of herbs and spices, have been described in relation to their in vivo anti-diabetic or anti-aging activity at physiological temperature. Under the hypothesis that those natural antioxidants may inhibit the formation of Maillard intermediates, the behavior of several hydroalcoholic plant extracts was analyzed in sugar-protein systems. Allspice, thyme, green pepper and black pepper extracts were the most efficient inhibitors, decreasing furosine formation by 60, 45, 40 and 30%, respectively. 5-hydroxymethyl-2-furfural formation decreased in the presence of the extracts and protein glycation was inhibited by the thyme extract in advanced stages. Antiglycation activities were related to polyphenols content, to radical scavenging and to iron-reducing power. In the protein-sugar systems studied at the time in which 4000 ppm of furosine were formed, the antioxidant activity dropped between 30 and 40%. Polyphenols inhibit Maillard intermediates formation, revealing the incidence of oxidative pathways, but they are depleted as a function of time.


Asunto(s)
Antioxidantes , Especias , Antioxidantes/análisis , Extractos Vegetales , Plantas Comestibles , Azúcares
10.
Psicothema (Oviedo) ; 34(3): 479-487, 2022. tab
Artículo en Inglés | IBECS | ID: ibc-207344

RESUMEN

Background: Young adolescents and pre-adolescents are the population most vulnerable to disorders derived from a distorted Body Image (BI). In this study, the Multidimensional Body Self Relations Questionnaire, MBSRQ®, was adapted and validated for young Spanish people between 9 and 16 years old. Method: 719 young people of both sexes participated. The internal structure, the invariance of the measure according to sex and age, and evidence of validity and reliability of the measure were examined. Results: the dimensional model found in adults was not replicated in young people. The simplest, best-fitting BI construct in young people, examined from the perspective of the BI construct contained in the MBSRQ®, consisted of 20 items in 4 differentially correlated factors. The internal consistency of the factors contained in the MBSRQ-SA-a was shown to be satisfactory, as was the evidence of concurrent validity. Factor invariance was demonstrated as a function of gender and three age groups. Conclusions: the MBSRQ-SA-a is reliable and valid for the study of BI in young people aged 9-16 years to the extent permitted by the content of the 4 factors making it up.(AU)


Antecedentes: los jóvenes adolescentes y preadolescentes son la población más vulnerable a los trastornos derivados de una Imagen Corporal (IC) distorsionada. En esta investigación se realiza la adaptación y validación del Multidimensional Body Self Relations Questionnaire, MBSRQ®, para jóvenes españoles entre 9 y 16 años. Método: participan 719 jóvenes de ambos sexos. Se estudia la estructura interna, la invarianza de la medida en función del sexo y de la edad, y la evidencia de validez y la fiabilidad de la medida. Resultados: el modelo dimensional hallado en los adultos no se replica en los jóvenes. El modelo más simple y mejor ajustado del constructo IC que tienen los jóvenes, examinado desde la óptica del constructo de IC contenida en el MBSRQ®, está formado por 20 ítems dimensionados en 4 factores diferencialmente correlacionados. Se demostró que la consistencia interna de los factores contenidos en el MBSRQ-SA-a es satisfactoria, y también lo es la evidencia de validez concurrente. Se demostró invarianza factorial en sexo y edad. Conclusiones: se concluye que el MBSRQ-SA-a es fiable y válido para el estudio de la IC en jóvenes entre 9-16 años en el alcance que permite el contenido de los 4 factores que lo conforman.(AU)


Asunto(s)
Humanos , Masculino , Femenino , Adolescente , Imagen Corporal , Reproducibilidad de los Resultados , Análisis Factorial , Satisfacción Personal , Encuestas y Cuestionarios , Psicometría/métodos
11.
Am J Ophthalmol ; 206: 17-31, 2019 10.
Artículo en Inglés | MEDLINE | ID: mdl-31163136

RESUMEN

PURPOSE: To evaluate whether high-resolution optical coherence tomography (HR-OCT) can detect histologic tumor margins of ocular surface squamous neoplasia (OSSN). METHODS: Eight eyes of 8 patients with OSSN undergoing excision were studied prospectively. Immediately before surgery, the tumor was imaged using commercially available HR-OCT to identify the conjunctival margins of the neoplastic lesion. The tumor borders of the lesion determined by HR-OCT were mapped in relation to an anatomic reference point and transferred intraoperatively. The tumor was excised with 4-mm margins from the visible edge of the lesion with a "no-touch" technique. The specimens were sent for pathologic analysis and the histologic tumor margin was compared to the HR-OCT predicted tumor border. RESULTS: Mean age of the 8 patients was 67 ± 9.9 years. Seven were male, 7 were white, and, ethnically, 3 were Hispanic. All 8 tumors were bulbar and in the exposure zone. Seven tumors were limbal. Corneal extension was present in 5. Mean tumor area was 17.5 ± 11.1 mm2. Clinically, 2 of the tumors were leukoplakic, 1 papillomatous, and 3 gelatinous. A conjunctival tumor margin identified with the HR-OCT coincided with the pathologically confirmed margin mark in all eyes. CONCLUSIONS: HR-OCT has the potential to predict histologic tumor margins in OSSN. Optical identification of tumor margins could potentially decrease the incidence of residual positive margins and minimize healthy tissue removal. Advances in HR-OCT technology and integration into a microscope for "real-time" imaging are needed to further improve this technique. NOTE: Publication of this article is sponsored by the American Ophthalmological Society.


Asunto(s)
Carcinoma de Células Escamosas/diagnóstico , Conjuntiva/patología , Córnea/patología , Neoplasias del Ojo/diagnóstico , Procedimientos Quirúrgicos Oftalmológicos/métodos , Cirugía Asistida por Computador/métodos , Tomografía de Coherencia Óptica/métodos , Anciano , Carcinoma de Células Escamosas/cirugía , Neoplasias del Ojo/cirugía , Femenino , Estudios de Seguimiento , Humanos , Imagenología Tridimensional , Masculino , Persona de Mediana Edad , Proyectos Piloto , Estudios Prospectivos
12.
Sci Rep ; 8(1): 17207, 2018 11 21.
Artículo en Inglés | MEDLINE | ID: mdl-30464290

RESUMEN

Willow (Salix spp.) seeds are able to tolerate desiccation, but differ from typical orthodox seeds in that they lose viability in a few days at room temperature, and in that the chloroplasts in embryo tissues do not dedifferentiate during maturation drying, thus retaining chlorophyll and maintaining intact their thylakoid membranes. In the present study, we investigated the damage generated in willow seeds during storage under appropriate conditions to exclude the eventual generation of reactive oxygen species by photooxidation. To this end, we measured different indicators of molecular damage, such as changes in the fatty acid profile, protein degradation, nuclease activities, and DNA damage, and evaluated normal germination and total germination in seeds stored for one, ten and sixteen years. We found: (i) a decrease in the fraction of unsaturated fatty acids; (ii) changes in the protein profile due to a decrease in protein solubility; (iii) activation of nucleases; and (iv) DNA fragmentation. Taken together, our findings identified programmed cell death as a key mechanism in seed deterioration during storage. We also found that, although the seeds maintained high percentages of total germination, the death program had already started in the seeds stored for ten years and was more advanced in those stored for sixteen years.


Asunto(s)
Germinación , Preservación Biológica , Salix/fisiología , Semillas/fisiología , ADN de Plantas/análisis , Desecación , Exposición a Riesgos Ambientales , Ácidos Grasos/análisis , Proteínas de Plantas/análisis , Salix/química , Semillas/química , Análisis de Supervivencia , Temperatura
13.
Food Chem ; 265: 86-95, 2018 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-29884399

RESUMEN

Thyme (Thymus vulgaris) has been demonstrated to extend the shelf-life of food products, being also a potential source of bioactive compounds. The aim of this research was to optimize the ultrasound assisted extraction employing ß-cyclodextrin aqueous solutions as no-contaminant technology and Response Surface Methodology to obtain thyme extracts with the maximum antioxidant capacity. The optimal extraction conditions were: a solution of ß-ciclodextrin 15 mM, an ultrasonic treatment time of 5.9 min at a temperature of 36.6 °C. They resulted in an extract with a polyphenolic content of 189.3 mg GAE/mL, an antioxidant activity (DPPH) of 14.8 mg GAE/mL, and ferric reducing/antioxidant power (FRAP) of 3.3 mg GAE/mL. Interestingly, the extract demonstrated to inhibit the production of Maillard browning products and can be considered a potential antiglycant agent. The obtained data is important for developing eco-friendly technologies in order to obtain natural antioxidant extracts with a potential inhibitory capacity of Maillard glycation reaction.


Asunto(s)
Fraccionamiento Químico/métodos , Reacción de Maillard , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Hojas de la Planta/química , Thymus (Planta)/química , beta-Ciclodextrinas/química , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Ondas Ultrasónicas
14.
Ophthalmic Surg Lasers Imaging Retina ; 47(10): 952-955, 2016 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-27759862

RESUMEN

A 6-day-old female baby with known diagnosis of congenital Zika infection was referred for ophthalmologic examination. The mother (37 years old) was referred for a pruritic rash, conjunctival hyperemia, and malaise at 12 weeks of gestation while still living in Venezuela. Upon arrival to Miami, Zika virus (ZIKV) exposure was confirmed during prenatal screening. At birth, due to the known exposure, a complete congenital ZIKV workup was performed, including brain ultrasound and MRI, which disclosed calcifications in the frontal lobe. Fundus examination revealed a hypopigmented retinal lesion in the left eye that was documented with retinal imaging. [Ophthalmic Surg Lasers Imaging Retina. 2016;47:952-955.].


Asunto(s)
Encéfalo/diagnóstico por imagen , Nervio Óptico/diagnóstico por imagen , Viaje , Infección por el Virus Zika/congénito , Virus Zika , Adulto , Femenino , Florida/epidemiología , Humanos , Recién Nacido , Imagen por Resonancia Magnética , Microcefalia , Síndrome , Venezuela/etnología , Infección por el Virus Zika/diagnóstico , Infección por el Virus Zika/etnología
15.
Plant Sci ; 238: 178-87, 2015 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-26259186

RESUMEN

During leaf senescence, degradation of chloroplasts precede to changes in nuclei and other cytoplasmic organelles, RuBisCO stability is progressively lost, grana lose their structure, plastidial DNA becomes distorted and degraded, the number of plastoglobuli increases and abundant senescence-associated vesicles containing electronically dense particles emerge from chloroplasts pouring their content into the central vacuole. This study examines quinoa leaf tissues during development and senescence using a range of well-established markers of programmed cell death (PCD), including: morphological changes in nuclei and chloroplasts, degradation of RuBisCO, changes in chlorophyll content, DNA degradation, variations in ploidy levels, and changes in nuclease profiles. TUNEL reaction and DNA electrophoresis demonstrated that DNA fragmentation in nuclei occurs at early senescence, which correlates with induction of specific nucleases. During senescence, metabolic activity is high and nuclei endoreduplicate, peaking at 4C. At this time, TEM images showed some healthy nuclei with condensed chromatin and nucleoli. We have found that DNA fragmentation, induction of senescence-associated nucleases and endoreduplication take place during leaf senescence. This provides a starting point for further research aiming to identify key genes involved in the senescence of quinoa leaves.


Asunto(s)
Chenopodium quinoa/citología , Chenopodium quinoa/crecimiento & desarrollo , Hojas de la Planta/citología , Hojas de la Planta/crecimiento & desarrollo , Núcleo Celular/metabolismo , Núcleo Celular/ultraestructura , Chenopodium quinoa/genética , Chenopodium quinoa/ultraestructura , Cloroplastos/metabolismo , Cloroplastos/ultraestructura , Fragmentación del ADN , Desoxirribonucleasas/metabolismo , Citometría de Flujo , Hojas de la Planta/genética , Hojas de la Planta/ultraestructura , Ploidias , Ribulosa-Bifosfato Carboxilasa/metabolismo
16.
J Integr Plant Biol ; 57(12): 996-1002, 2015 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-25953251

RESUMEN

During the diversification of angiosperms, seeds have evolved structural, chemical, molecular and physiologically developing changes that specially affect the nucellus and endosperm. All through seed evolution, programmed cell death (PCD) has played a fundamental role. However, examples of PCD during seed development are limited. The present review examines PCD in integuments, nucellus, suspensor and endosperm in those representative examples of seeds studied to date.


Asunto(s)
Apoptosis , Magnoliopsida/citología , Magnoliopsida/embriología , Semillas/citología , Endospermo/citología , Endospermo/embriología , Magnoliopsida/enzimología , Semillas/enzimología
17.
Rev. Univ. Ind. Santander, Salud ; 46(3): 259-276, Diciembre 18, 2014. graf, tab
Artículo en Español | LILACS-Express | LILACS | ID: lil-742707

RESUMEN

Introducción: La ergonomía puede contribuir al diseño de puestos, entornos y sistemas para hacerlos compatibles con las necesidades, capacidades y limitaciones de las personas. Como respuesta a las necesidades actuales y al desafío que tienen los diseñadores y ergónomos de facilitar y mejorar la calidad de vida de las personas. Objetivos: Elaborar un diagnóstico de la accesibilidad de 34 espacios públicos privados de la ciudad de Temuco. Materiales y Métodos: Se diseñó para este fin un instrumento tipo ficha que permitió registrar las observaciones hechas en terreno teniendo incorporados los criterios de accesibilidad contemplados en la legislación y normativa vigente, y aquellos recomendados por especialistas internacionales que van más allá de la norma chilena. Resultados: Se obtuvieron datos objetivos que permitieron realizar un análisis de accesibilidad de índole cuantitativo y cualitativo representativo de la ciudad que ilustra la realidad actual de la capital regional de la novena región. El instrumento permitió anexar fotografías y recomendaciones para mejorar la accesibilidad, sistematizando de forma ordenada y gráfica la información. Conclusiones: Desde un enfoque holístico se dan pautas para lograr el ajuste y la adecuación de los recintos observados en las dimensiones de entorno, entrada, zona de atención al público, ascensor y aseos públicos a los requerimientos y capacidades de personas con discapacidad.


Introduction: The ergonomics can contribute to job design, environments and systems to make them compatible with needs, abilities and limitations of people. In response to the current needs and the challenge that are facing designers and ergonomists to facilitate and improve the quality of life of people. Objectives: Elaborate a diagnosis of the accessibility of 34 public and private spaces of the city of Temuco. Materials and Methods: We designed for this purpose an instrument type record card that allowed recording observations made in the field, having incorporated accessibility criteria under the laws and regulations actives, and those recommended by international experts who go beyond the Chilean norm. Results: Objective data were obtained that allowed an analysis of accessibility of quantitative and qualitative nature representative of the city that illustrate the current reality of the regional capital of the Ninth region. The tool enables to attach photographs and recommendations for improving accessibility, systematizing the information in an orderly and graphical way. Conclusions: From a holistic approach are given guidelines to ensure the fit and adequacy of the enclosures observed in the dimensions of environment, entry, customer service area, elevators and public toilets the needs and abilities of people with disabilities.

18.
Ophthalmic Surg Lasers Imaging Retina ; 44 Online(6): e7-10, 2013 Oct 09.
Artículo en Inglés | MEDLINE | ID: mdl-24094528

RESUMEN

After being exposed to an industrial Nd:YAG laser, an 11-year-old boy developed a full-thickness macular hole in the right eye with resultant visual acuity of 20/100. Five months after the initial injury, the vision was stable and the hole was still present. The patient underwent pars plana vitrectomy, internal limiting membrane peeling, and gas tamponade with prone positioning. Three months postoperatively, visual acuity improved to 20/25 with closure of the macular hole; however, there was a focal photoreceptor deficit. A review of the English-language literature identified 15 eyes with similar macular hole injuries after Nd:YAG exposure. Three eyes were surgically managed. While all holes were successfully closed, only two experienced visual improvement.


Asunto(s)
Láseres de Estado Sólido/efectos adversos , Perforaciones de la Retina/etiología , Niño , Humanos , Masculino , Resultado del Tratamiento , Vitrectomía
19.
J Exp Bot ; 64(11): 3313-25, 2013 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-23833197

RESUMEN

At seed maturity, quinoa (Chenopodium quinoa Willd.) perisperm consists of uniform, non-living, thin-walled cells full of starch grains. The objective of the present study was to study quinoa perisperm development and describe the programme of cell death that affects the entire tissue. A number of parameters typically measured during programmed cell death (PCD), such as cellular morphological changes in nuclei and cytoplasm, endoreduplication, DNA fragmentation, and the participation of nucleases and caspase-like proteases in nucleus dismantling, were evaluated; morphological changes in cytoplasm included subcellular aspects related to starch accumulation. This study proved that, following fertilization, the perisperm of quinoa simultaneously accumulates storage reserves and degenerates, both processes mediated by a programme of developmentally controlled cell death. The novel findings regarding perisperm development provide a starting point for further research in the Amaranthaceae genera, such as comparing seeds with and without perisperm, and specifying phylogeny and evolution within this taxon. Wherever possible and appropriate, differences between quinoa perisperm and grass starchy endosperm--a morphologically and functionally similar, although genetically different tissue--were highlighted and discussed.


Asunto(s)
Chenopodium quinoa/citología , Chenopodium quinoa/metabolismo , Semillas/citología , Semillas/metabolismo , Apoptosis/genética , Apoptosis/fisiología , Chenopodium quinoa/genética , Fragmentación del ADN , Péptido Hidrolasas/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Semillas/genética
20.
Plant Cell Rep ; 31(12): 2139-49, 2012 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-22868443

RESUMEN

UNLABELLED: The objective of the present study was to determine dehydrin protein levels in sugarcane var. SP80-3280 during somatic embryogenesis. Dehydrins from embryogenic and non-embryogenic cell cultures were analyzed using western blot and in situ immunolocalization microscopy. Both techniques employ antibodies raised against a highly conserved lysine-rich 15-amino acid sequence termed the K-domain, which is extensively used to recognize proteins immunologically related to the dehydrin family. In embryogenic cultures, western blot analysis of the heat-stable protein fraction revealed eleven major bands ranging from 52 to 17 kDa. They were already visible on the first days, gradually increasing until reaching peak values around day 14, when organogenesis begins, to later decrease in concurrence with the appearance of green plantlets (around day 28). These fluctuations indicate that this pattern of accumulation is under developmental control. Dehydrins were mainly immunolocalized in the nuclei. A phosphatase treatment of protein extracts caused a mobility shift of the 52, 49, and 43 kDa dehydrin bands suggesting a putative modulation mechanism based on protein phosphorylation. In sugarcane embryogenic cultures, presence of dehydrins is a novel finding. Dehydrins were absent in non-embryogenic cultures. The novel findings regarding accumulation, nuclear localization, and phosphorylation of dehydrins provide a starting point for further research on the role of these proteins in the induction and/or maintenance of embryogenesis. KEY MESSAGE: The novel findings regarding accumulation, nuclear localization, and phosphorylation of dehydrins provide a starting point for further research on the role of these proteins in the induction and/or maintenance of embryogenesis.


Asunto(s)
Proteínas de Plantas/metabolismo , Técnicas de Embriogénesis Somática de Plantas/métodos , Saccharum/metabolismo , Secuencia de Aminoácidos , Western Blotting , Núcleo Celular/metabolismo , Inmunohistoquímica , Lisina/metabolismo , Meristema/crecimiento & desarrollo , Meristema/metabolismo , Microscopía Electrónica de Transmisión , Fosforilación , Estabilidad Proteica , Estructura Terciaria de Proteína , Saccharum/anatomía & histología , Saccharum/crecimiento & desarrollo , Semillas/crecimiento & desarrollo , Semillas/metabolismo , Semillas/ultraestructura
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