Detection of carbapenemase- and ESBL-producing Klebsiella pneumoniae from bovine bulk milk and comparison with clinical human isolates in Italy.

Klebsiella pneumoniae is the most common Klebsiella species infecting animals and is one of the causing agents of mastitis in cows. The rise of antimicrobial resistance in K. pneumoniae, particularly in strains producing extended-spectrum ß-lactamases (ESBLs) and/or carbapenemases, is of concern worldwide. Recently (Regulation UE No 2022/1255), carbapenems and cephalosporins in combination with ß-lactamase inhibitors have been reserved only to human treatments in the European Union. The aim of this study was to investigate the role of cattle as carrier of human pathogenic carbapenem-resistant (CR) and ESBL-producing K. pneumoniae. On this purpose, a study involving 150 dairy farms in Parma province (Northern Italy) and 14 non replicate K. pneumoniae isolates from patients admitted at Parma University-Hospital was planned. Four multidrug resistant (MDR) K. pneumoniae strains were detected from 258 milk filters collected between 2019 and 2021. One carbapenemase KPC-3-positive K. pneumoniae ST307 (0.4 %; 95 % CI - 0.07 - 2.2) was detected in milk filters. The isolate also harboured OXA-9, CTX-M-15 and SHV-106 determinants, together with genes conferring resistance to aminoglycosides (aac(3')-IIa, aph (3″)-Ib, aph (6)-Id), fluoroquinolones (oqxA, oqxB, qnrB1), phosphonic acids (fosA6), sulphonamides (sul2), tetracyclines (tet(A)6) and trimethoprim (dfrA14). One KPC-3-producing K. pneumoniae ST307 was identified also among the human isolates, thus suggesting a possible circulation of pathogens out of the clinical settings. The remaining three bovine isolates were MDR ESBL-producing K. pneumoniae characterized by different genomic profiles: CTX-M-15, TEM-1B and SHV-187 genes (ST513); CTX-M-15 and SHV-145 (ST307); SHV-187 and DHA-1 (ST307). Occurrence of ESBL-producing K. pneumoniae in milk filters was 1.2 % (95 % CI 0.4-3.4). All the isolates showed resistance to aminoglycosides, 3rd-generation cephalosporins, and fluoroquinolones. Among the human isolates, two multidrug resistant ESBL-producing K. pneumoniae ST307 were found, thus confirming the circulation of this high-risk lineage between humans and cattle. Our findings suggest that food-producing animals can carry human pathogenic microorganisms harboring resistance genes against carbapenems and 3rd-generation cephalosporins, even if not treated with such antimicrobials. Moreover, on the MDR K. pneumoniae farms, the antimicrobial use was much higher than the Italian median value, thus highlighting the importance of a more prudent use of antibiotics in animal productions.

Efeitos da obesidade na distribuição de pressão plantar em crianças / Effects of obesity on plantar pressure distribution in children

OBJETIVO: O estudo teve como objetivo determinar se há diferenças na distribuição de pressão plantar estática e dinâmica entre crianças obesas e eutróficas. MÉTODO: Foram avaliadas vinte crianças, divididas em dois grupos (grupo de obesos e grupo de eutróficos), com idades entre nove e onze anos. As avaliações incluíram medidas das variáveis de pressão plantar na postura ereta e na marcha por meio do sistema Pedar (Novel GMbH). RESULTADOS: Constatou-se que as crianças obesas apresentaram maiores áreas de contato, picos de pressão, pressões médias máximas e integrais pressão-tempo, quando comparadas às eutróficas, com diferenças significativas, principalmente nas regiões do médio-pé e antepé. CONCLUSÕES: As diferenças observadas entre os grupos indicam que crianças obesas podem apresentar modificações importantes nos pés em função da sobrecarga excessiva e repetitiva à qual estão expostas, aumentando o risco para o desenvolvimento de lesões e patologias nos pés. Portanto, é necessário que programas de intervenção sejam implantados a fim de interferir também na progressão de problemas de natureza estrutural e funcional relacionados à obesidade.

OBJECTIVE: The aim of this study was to determine whether there were differences in static and dynamic plantar pressure distribution between obese and non-obese children. METHOD: Twenty children aged from nine to eleven years were assessed and divided into two groups (obese and non-obese groups). The assessments included measurements of plantar pressure variables while standing and walking, by means of the Pedar System (Novel GMbH). RESULTS: The obese children presented greater contact area, peak pressure, maximum mean pressure and pressure-time integral, in comparison with the non-obese children, with significant differences particularly in the midfoot and forefoot areas. CONCLUSION: The differences observed between the groups indicated that obese children may present significant modifications to their feet because of the excessive and repetitive loads that they are exposed to, which increases the risk of developing foot injuries and pathologies. It is suggested that there is a need to implement intervention programs with the aim of interfering with the progression of obesity-related problems from a structural and functional perspective.