RESUMEN
River water temperatures are increasing globally, particularly in urban systems. In winter, wastewater treatment plant (WWTP) effluent inputs are of particular concern because they increase water temperatures from near freezing to ~7-15 °C. Recent laboratory studies suggest that warm overwinter temperatures impact the reproductive timing of some fishes. To evaluate winter water temperature's influence in the wild, we sampled Johnny Darter Etheostoma nigrum from three urban South Platte River tributaries in Colorado upstream and downstream of WWTP effluent discharge sites. Fish were collected weekly during the spring spawning season of 2021 and reproductive development was determined from histological analysis of the gonads. Winter water temperatures were approximately 5-10 °C greater ~300 m downstream of the WWTP effluent compared to upstream sites, and approximately 3°C warmer at sampling sites ~5000 m downstream of the effluent discharge. Females collected downstream of WWTP effluent experienced accelerated reproductive development compared to upstream by 1-2 weeks. Water quality, including total estrogenicity, and spring water temperatures did not appear to explain varying reproductive development. It appears that small increases in winter water temperature influence the reproductive timing in E. nigrum. Further investigations into how shifts in reproductive timing influence other population dynamics are warranted.
RESUMEN
Fisheries biologists have been hesitant to use passive integrated transponder (PIT) tags in small-bodied fishes (40-200 mm TL) such as darters (Percidae: Etheostomatinae) because of the fishes' size and potential effect on swimming performance. The authors used constant acceleration trials to evaluate the swimming performance of Arkansas darters Etheostoma cragini in control (no incision or tag), sham (incision and suture) or PIT tagged (surgically implanted 8 × 1.4 mm intra-peritoneal PIT tag) treatments. Tag retention and fish survival were monitored for up to 199 days post-tagging. Maximum swimming velocity did not differ between control, sham and PIT tag treatments, nor was maximum swimming velocity affected by the tagging procedure. Tag retention was 100%, and the overall survival of tagged fish was 88% in the swimming study, and 100% in the long-term study, suggesting that small PIT tags are suitable for use in darters. The authors include a brief meta-analysis on the results reported by 20 studies that PIT tagged small-bodied fishes, representing 38 species and nine families of freshwater fish.
Asunto(s)
Percas , Natación , Aceleración , Animales , Explotaciones PesquerasRESUMEN
Context: Autonomic dysreflexia is a potentially lethal condition experienced by spinal cord injury (SCI) patients. It has a wide range of causes, most of which are genitourinary in nature. However, there has been no documented case of primary bladder cancer as a trigger for recurrent autonomic dysreflexia. Findings: We present a case of a 51-year-old spinal cord injury patient with multiple presentations of autonomic dysreflexia. Work up revealed locally invasive bladder cancer, and following definitive surgery, his symptoms were alleviated. Conclusions: In SCI patients with recurrent AD, comprehensive work up with involvement of a specialized Urologist is required as AD may be the only presenting symptom of a serious underlying medical condition.
Asunto(s)
Disreflexia Autónoma , Traumatismos de la Médula Espinal , Neoplasias de la Vejiga Urinaria , Disreflexia Autónoma/diagnóstico , Disreflexia Autónoma/etiología , Humanos , Músculos , Neoplasias de la Vejiga Urinaria/complicaciones , Neoplasias de la Vejiga Urinaria/diagnósticoRESUMEN
An in-person multidisciplinary continuing medical education (CME) program was designed to address previously identified knowledge gaps regarding quality indicators of care in kidney cancer. The objective of this study was to develop a CME program and determine if the program was effective for improving participant knowledge. CME programs for clinicians were delivered by local experts (uro-oncologist and medical oncologist) in four Canadian cities. Participants completed knowledge assessment tests pre-CME, immediately post-CME, and 3-month post-CME. Test questions were related to topics covered in the CME program including prognostic factors for advanced disease, surgery for advanced disease, indications for hereditary screening, systemic therapy, and management of small renal masses. Fifty-two participants attended the CME program and completed the pre- and immediate post-CME tests. Participants attended in Ottawa (14; 27%), Toronto (13; 25%), Québec City (18; 35%), and Montréal (7; 13%) and were staff urologists (21; 40%), staff medical oncologists (9; 17%), fellows (5; 10%), residents (16; 31%), and oncology nurses (1; 2%). The mean pre-CME test score was 61% and the mean post-CME test score was 70% (p = 0.003). Twenty-one participants (40%) completed the 3-month post-CME test. Of those that completed the post-test, scores remained 10% higher than the pre-test (p value 0.01). Variability in test scores was observed across sites and between French and English test versions. Urologists had the largest specialty-specific increase in knowledge at 13.8% (SD 24.2, p value 0.02). The kidney cancer CME program was moderately effective in improving provider knowledge regarding quality indicators of kidney cancer care. These findings support continued use of this CME program at other sites.
Asunto(s)
Carcinoma de Células Renales/diagnóstico , Carcinoma de Células Renales/terapia , Detección Precoz del Cáncer/estadística & datos numéricos , Educación Médica Continua/normas , Neoplasias Renales/diagnóstico , Neoplasias Renales/terapia , Investigación Biomédica Traslacional , Canadá/epidemiología , Carcinoma de Células Renales/epidemiología , Implementación de Plan de Salud , Humanos , Neoplasias Renales/epidemiologíaRESUMEN
INTRODUCTION: In competency-based models of residency training, work-based assessments of residents' technical skills are essential both for providing formative feedback and for assessing surgical competence. The Ottawa Surgical Competency Operating Room Evaluation (O-SCORE) is a previously validated paper-based evaluation tool created to assess a surgical trainees' operative competence. To address some of the barriers to assessment, we developed and implemented a mobile application that combines the O-SCORE with a surgical case log. METHODS: A description of the development implementation process for the mobile O-SCORE and case log is provided. Following implementation, a survey was developed and administered electronically to all faculty and residents within the University of Ottawa's Division of Urology to assess user perceptions and utilization of the application. The survey was administered and data collected via Survey Monkey. RESULTS: The overall response rate was 94%. The majority of residents (94%) reported that it was easy to log cases with the application and 81% felt that it had a positive impact on their training; 75% of faculty were willing or very willing to complete evaluations when assigned and 66% felt that the application had a positive effect on the quality of feedback they provided. CONCLUSIONS: Overall, faculty and residents felt that our mobile O-SCORE application was user-friendly and valuable as both a surgical log and assessment tool. With surgical programs moving towards competency-based models of training and assessment, the O-SCORE mobile application represents a practical electronic surgical log and work-based assessment instrument that can be easily adopted into any surgical training program.
RESUMEN
PURPOSE: The holmium laser is used increasingly for a wide array of urological procedures. Laser safety goggles are mandatory at many centers for individuals within the nominal hazard zone, as set out by the institution. Recent ex vivo studies suggest standard eye wear may be equally as protective. We sought to evaluate the perceptions and practice patterns of laser safety goggles in urology. MATERIALS AND METHODS: A 24-question survey was sent out through e-mail to an international e-mail list of â¼2000 urologists that were members of the Endourological Society. Data were collected anonymously using Survey Monkey. RESULTS: A total of 264 (14%) urologists completed the survey. Thirty-four percent worked in the community, whereas 63% worked at an academic institution. Ninety-seven percent routinely used the holmium laser. The most common uses were lithotripsy (99%), tissue incision (71%), tumor ablation (58%), and prostate ablation (26%). Formal laser training and institutional laser safety policies were reported in 76% and 64%, respectively. Forty percent of respondents routinely wore laser safety goggles. Laser adverse events were witnessed by 19%, but there were no eye injuries reported. Seventy percent of surgeons felt that laser safety goggles may impair their vision. When presented with the information that regular eye glasses may be as effective as laser goggles for preventing harm, the majority (86%) would opt for regular eye wear. CONCLUSIONS: Laser safety eyewear practice patterns vary greatly. Many centers have adopted policies for universal mandatory laser goggles in the operating room. With over two thirds of surgeons suggesting laser goggles impair their vision, and recent literature suggesting regular eye wear is equivalent in preventing laser-associated eye injuries, laser goggle safety policies should be updated to better match the potential hazards inherent to the device.
Asunto(s)
Lesiones Oculares/prevención & control , Láseres de Estado Sólido/efectos adversos , Pautas de la Práctica en Medicina , Urólogos , Canadá , Estudios de Cohortes , Femenino , Humanos , Masculino , Encuestas y CuestionariosRESUMEN
Amyloidosis is a protein folding disorder characterized by the deposition of fibrillar proteins into solid organs or tissues. Primary localized amyloidosis of the bladder is very rare and can mimic bladder cancer in its presentation with hematuria, lower urinary tract symptoms or a mass on imaging. A case of localized amyloidosis of the bladder in a 48-year-old man with painless gross hematuria and evidence of bladder mass on ultrasound is presented. Amyloidosis is a rare but important non-malignant process of the bladder. We present a review of the literature and suggestions for management of this rare bladder disease.
Asunto(s)
Amiloidosis/diagnóstico por imagen , Enfermedades de la Vejiga Urinaria/diagnóstico por imagen , Neoplasias de la Vejiga Urinaria/diagnóstico por imagen , Amiloidosis/complicaciones , Amiloidosis/patología , Biopsia , Cistoscopía , Diagnóstico Diferencial , Hematuria/etiología , Humanos , Masculino , Persona de Mediana Edad , Tomografía Computarizada por Rayos X , Ultrasonografía , Enfermedades de la Vejiga Urinaria/complicaciones , Enfermedades de la Vejiga Urinaria/patología , UrografíaRESUMEN
INTRODUCTION: In this study we determined self-perceived knowledge gaps and continuing medical education preferences among Canadian urologists and medical oncologists related to the treatment of patients with kidney cancer. METHODS: A needs assessment survey was created by the Quality Initiative group of the Kidney Cancer Research Network of Canada using an iterative feedback process. The survey determined knowledge gaps and continuing medical education preferences pertaining to 23 previously validated quality indicators of kidney cancer care. Topics included screening, diagnosis, prognosis, surgical management, systemic therapies and followup care. The survey was distributed via e-mail to Canadian urologists and medical oncologists. RESULTS: Among the 164 respondents 121 (74%) were urologists and 43 (26%) were medical oncologists. The majority of respondents practice in academic (72, 57%) or large urban community centers (40, 32%). Of the 23 quality indicators examined 14 were designated as priority continuing medical education topics based on perceived inadequate knowledge or high interest in the topic. Priority topics were similar for urologists and medical oncologists, and covered the spectrum of kidney cancer care with an emphasis on hereditary kidney cancer and management of advanced disease. Most respondents preferred that continuing medical education be delivered through in person, case based group discussions. CONCLUSIONS: Canadian urologists and medical oncologists report similar knowledge gaps and continuing medical education preferences regarding kidney cancer care. Priority topics include screening for hereditary kidney cancer and management of advanced disease.
RESUMEN
Keratinizing desquamative squamous metaplasia (KDSM) in the renal pelvis is a rare condition with unclear malignant potential. Recent reports suggest it is likely benign and favor endoscopic treatment approaches. Medical record review was completed on two cases at our center to obtain history, physical examination, radiographic findings, and management. A literature review was completed to identify all published cases of KDSM. Both patients at our center suffered recurrent urolithiasis, hypothesized to be secondary to KDSM. Both were managed with a percutaneous approach to ensure complete stone and KDSM plaque removal. Our cases highlight that percutaneous surgery is an excellent management option for stone and KDSM eradication from the collecting system. This approach also allows adequate oncologic surveillance of the underlying urothelium.
Asunto(s)
Pelvis Renal , Nefrolitiasis , Urotelio , Biopsia/métodos , Femenino , Humanos , Queratinas/metabolismo , Pelvis Renal/diagnóstico por imagen , Pelvis Renal/patología , Litotricia/métodos , Masculino , Metaplasia , Persona de Mediana Edad , Nefrolitiasis/complicaciones , Nefrolitiasis/diagnóstico , Nefrolitiasis/fisiopatología , Nefrolitiasis/cirugía , Recurrencia , Tomografía Computarizada por Rayos X/métodos , Resultado del Tratamiento , Urotelio/diagnóstico por imagen , Urotelio/metabolismo , Urotelio/patologíaRESUMEN
While multi-species occupancy models (MSOMs) are emerging as a popular method for analyzing biodiversity data, formal checking and validation approaches for this class of models have lagged behind. Concurrent with the rise in application of MSOMs among ecologists, a quiet regime shift is occurring in Bayesian statistics where predictive model comparison approaches are experiencing a resurgence. Unlike single-species occupancy models that use integrated likelihoods, MSOMs are usually couched in a Bayesian framework and contain multiple levels. Standard model checking and selection methods are often unreliable in this setting and there is only limited guidance in the ecological literature for this class of models. We examined several different contemporary Bayesian hierarchical approaches for checking and validating MSOMs and applied these methods to a freshwater aquatic study system in Colorado, USA, to better understand the diversity and distributions of plains fishes. Our findings indicated distinct differences among model selection approaches, with cross-validation techniques performing the best in terms of prediction.
Asunto(s)
Biodiversidad , Peces/fisiología , Modelos Estadísticos , Animales , Teorema de Bayes , Colorado , Conservación de los Recursos Naturales , Ecosistema , Peces/clasificaciónRESUMEN
Gene duplication and positive selection can be important determinants of the evolution of venom, a protein-rich secretion used in prey capture and defense. In a typical model of venom evolution, gene duplicates switch to venom gland expression and change function under the action of positive selection, which together with further duplication produces large gene families encoding diverse toxins. Although these processes have been demonstrated for individual toxin families, high-throughput multitissue sequencing of closely related venomous species can provide insights into evolutionary dynamics at the scale of the entire venom gland transcriptome. By assembling and analyzing multitissue transcriptomes from the Western black widow spider and two closely related species with distinct venom toxicity phenotypes, we do not find that gene duplication and duplicate retention is greater in gene families with venom gland biased expression in comparison with broadly expressed families. Positive selection has acted on some venom toxin families, but does not appear to be in excess for families with venom gland biased expression. Moreover, we find 309 distinct gene families that have single transcripts with venom gland biased expression, suggesting that the switching of genes to venom gland expression in numerous unrelated gene families has been a dominant mode of evolution. We also find ample variation in protein sequences of venom gland-specific transcripts, lineage-specific family sizes, and ortholog expression among species. This variation might contribute to the variable venom toxicity of these species.
Asunto(s)
Glándulas Endocrinas/metabolismo , Evolución Molecular , Duplicación de Gen , Selección Genética , Venenos de Araña/genética , Arañas/genética , Transcriptoma , Animales , Femenino , Arañas/metabolismoRESUMEN
OBJECTIVE: To determine cervical cancer screening coverage and the knowledge, attitudes and barriers toward screening tests among women in rural and urban areas of Tanzania, as well as explore how they view the acceptability of the HPV vaccine and potential barriers to vaccination. SETTING: A cross-sectional study using interview-administered questionnaires was conducted using multistage random sampling within urban and rural areas in Kilimanjaro Region, Tanzania. PARTICIPANTS: Women aged 18-55 were asked to participate in the survey. The overall response rate was 97.5%, with a final sample of 303 rural and 272 urban dwelling women. PRIMARY AND SECONDARY OUTCOME MEASURES: Descriptive and simple test statistics were used to compare across rural and urban strata. Multivariate logistic regression models were used to estimate ORs and 95% CIs. RESULTS: Most women (82%) reported they had heard of cervical cancer, while self-reported cervical cancer screening among women was very low (6%). In urban areas, factors associated with screening were: older age (OR=4.14, 95% CI 1.86 to 9.24 for ages 40-49, and OR=8.38, 95% CI 2.10 to 33.4 for >50â years), having health insurance (OR=4.15, 95% CI 1.52 to 11.4), and having knowledge about cervical cancer (OR=5.81, 95% CI 1.58 to 21.4). In contrast, among women residing in rural areas, only condom use (OR=6.44, 95% CI 1.12 to 37.1) was associated with screening. Women from both rural and urban areas had low vaccine-related knowledge; however, most indicated they would be highly accepting if it were readily available (93%). CONCLUSIONS: The current proportion of women screened for cervical cancer is very low in Kilimanjaro Region, and our study has identified several modifiable factors that could be addressed to increase screening rates. Although best implemented concurrently, the availability of prophylactic vaccination for girls may provide an effective means of prevention if they are unable to access screening in the future.