Aplicación de un programa Psico-organizacional y su efecto en personal con Síndrome de Burnout / Aplicação de um programa Psico-organizacional e seu efeito em pessoas com Síndrome de Burnout / Application of a Psycho-organizational program and its effect on personnel with Burnout Syndrome

Resumen El síndrome psicosocial en estudio se genera por la presencia de ambientes con elevados niveles de estrés por la relación perenne con diferentes individuos durante el tiempo en que está en su actividad profesional. El diseño de la investigación fue pre experimental, teniéndose como variable independiente el programa Psico-organizacional aplicado en las dimensiones efectividad de la conducta humana, efectividad organizacional y desarrollo cultural en el trabajo, y la variable dependiente el Síndrome de Burnout aplicado en las dimensiones Agotamiento personal, despersonalización y realización personal. Se determinó el efecto del programa Psico-organizacional en el personal que laboraba en la Micro Red Baños del Inca perteneciente al Ministerio de Salud de Perú, 2019 quienes mostraron síntomas de padecer del síndrome. La muestra estuvo constituida por 65 empleados. Según el Pre test antes de la aplicación del programa Psico-organizacional los empleados presentaron en que el 93,8% presentó un nivel medio/alto del síndrome, en comparación de un 64.6% post aplicación del programa que presentó un nivel bajo del síndrome. La aplicación del programa Psico-organizacional en los empleados de la Micro Red de Salud Baños del Inca, mejoró sus niveles de estrés expresado en la reducción de los niveles del síndrome de Burnout.

Resumo A síndrome psicossocial em estudo é gerada pela presença de ambientes com elevados níveis de stress devido à relação perene com diferentes indivíduos durante o tempo em que estes se encontram na sua atividade profissional. O desenho da investigação foi pré-experimental, tendo como variável independente o programa Psico-organizacional aplicado nas dimensões eficácia do comportamento humano, eficácia organizacional e desenvolvimento cultural no trabalho, e a variável dependente a Síndrome de Burnout aplicada nas dimensões Exaustão Pessoal, Despersonalização e Realização Pessoal. Foi determinado o efeito do programa Psico-organizacional sobre o pessoal que trabalha no Micro Red Baños del Inca pertencente ao Ministério da Saúde do Peru, em 2019, que apresentava sintomas de sofrimento por esta síndrome. A amostra era composta por 65 trabalhadores. De acordo com o pré-teste antes da aplicação do programa Psico-organizacional, 93,8% dos colaboradores apresentavam um nível médio/alto da síndrome, contra 64,6% após a aplicação do programa, que apresentavam um nível baixo da síndrome. A aplicação do programa Psico-organizacional nos colaboradores da Micro Red de Salud Baños del Inca melhorou os seus níveis de estresse expressos na redução dos níveis da Síndrome de Burnout.

Abstract The psychosocial syndrome under study is generated by the presence of environments with high levels of stress due to the perennial relationship with different individuals during the time they are in their professional activity. The design of the investigation was pre-experimental, having the Psycho-organizational program as an independent variable applied in the effectiveness of human behavior, organizational effectiveness, and cultural development at work dimensions, and the dependent variable the Burnout Syndrome applied in the Personal Exhaustion, Depersonalization, and Personal Achievement dimensions. The effect of the Psycho-organizational program on the personnel working in the Micro Red Baños del Inca belonging to the Ministry of Health of Peru, 2019 who showed symptoms of suffering from the syndrome was determined. The sample consisted of 65 employees. According to the pre-test before the application of the Psycho-organizational program, 93.8% of the employees presented a medium/high level of the syndrome, compared to 64.6% after the application of the program who presented a low level of the syndrome. The application of the Psycho-organizational program in the employees of the Micro Red de Salud Baños del Inca improved their stress levels expressed in the reduction of the levels of the Burnout syndrome.

Plasma alterations in cholinergic and serotonergic systems in early Alzheimer Disease: Diagnosis utility.

BACKGROUND: Alzheimer Disease (AD) is the most common cause of dementia and it involves a high social and economic cost worldwide, and the health system still does not count with an effective treatment. This may be explained by the lack of a reliable early diagnosis and the complex physiological mechanisms involved in the disease development. In this sense, the cholinergic and serotonergic systems may be altered in the disease course. METHODS: In this study, metabolites from these pathways were determined in order to develop a non-invasive and early diagnosis model, as well as to advance in the knowledge of the physiopathological mechanisms of the disease. For this, plasma samples from mild cognitive impairment due to AD patients (MCI-AD, n = 25) and healthy controls (n = 25) were analysed. RESULTS: choline and tryptophan pathways were deregulated in MCI-AD. Therefore, a model based on betaine, cytidine, uridine, choline, acetylcholine, serotonin and tryptophan was developed, showing an AUC-ROC of 0.862, and sensitivity and specificity of 96% and 72%, respectively. CONCLUSION: Alterations in metabolites from these pathways are related to cognitive impairment and neurodegeneration, and they could be useful in AD diagnosis. Nevertheless, further research is required in order to validate this diagnosis model.

Rabbit haemorrhagic disease virus 2 (RHDV2) outbreak in Azores: Disclosure of common genetic markers and phylogenetic segregation within the European strains.

Rabbit haemorrhagic disease virus 2 (RHDV2) is widespread in several countries of Western Europe, but it has not been introduced to other continents. However, between late 2014 and early 2015, the presence of RHDV2 was confirmed outside of the European continent, in the Azores, initially in the islands of Graciosa, Flores, S. Jorge and Terceira. In this study we report the subsequent detection of RHDV2 in wild rabbits from the islands of Faial, St. Maria and S. Miguel, and display the necropsy and microscopic examination data obtained, which showed lesions similar to those induced by classical strains of RHDV, with severe affection of lungs and liver. We also disclose the result of a genetic investigation carried out with RHDV2 positive samples from wild rabbits found dead in the seven islands. Partial vp60 sequences were amplified from 27 tissue samples. Nucleotide analysis showed that the Azorean strains are closely related to each other, sharing a high genetic identity (>99.15%). None of the obtained sequences were identical to any RHDV2 sequence publically known, hampering a clue for the source of the outbreaks. However, Bayesian and maximum likelihood phylogenetic analyses disclosed that Azorean strains are more closely related to a few strains from Southern Portugal than with any others presently known. In the analysed region comprising the terminal 942 nucleotides of the vp60 gene, four new single nucleotide polymorphisms (SNP) were identified. Based on the present data, these four SNPs, which are unique in the strains from Azores, may constitute putative molecular geographic markers for Azorean RHDV2 strains, if they persist in the future. One of these variations is a non-synonymous substitution that involves the replacement of one amino acid in a hypervariable region of the capsid protein.

MLVA16 typing of Portuguese human and animal Brucella melitensis and Brucella abortus isolates.

To investigate the epidemiological relationship of isolates from different Portuguese geographical regions and to assess the diversity among isolates, the MLVA16(Orsay) assay (panels 1, 2A and 2B) was performed with a collection of 126 Brucella melitensis (46 human and 80 animal isolates) and 157 B. abortus field isolates, seven vaccine strains and the representative reference strains of each species. The MLVA16(Orsay) showed a similar high discriminatory power (HGDI 0.972 and 0.902) for both species but panel 1 and 2A markers displayed higher diversity (HGDI 0.693) in B. abortus compared to B. melitensis isolates (HGDI 0.342). The B. melitensis population belong to the "Americas" (17%) and "East Mediterranean" (83%) groups. No isolate belonged to the "West Mediterranean" group. Eighty-five percent of the human isolates (39 in 46) fit in the "East-Mediterranean" group where a single lineage known as MLVA11 genotype 116 is responsible for the vast majority of Brucella infections in humans. B. abortus isolates formed a consistent group with bv1 and bv3 isolates in different clusters. Four MLVA11 genotypes were observed for the first time in isolates from S. Jorge and Terceira islands from Azores. From the collection of isolates analysed in this study we conclude that MLVA16(Orsay) provided a clear view of Brucella spp. population, confirming epidemiological linkage in outbreak investigations. In particular, it suggests recent and ongoing colonisation of Portugal with one B. melitensis lineage usually associated with East Mediterranean countries.