Neutral Formazan Ligands Bound to the fac-(CO)3Re(I) Fragment: Structural, Spectroscopic, and Computational Studies.

Metal complexes with ligands that coordinate via the nitrogen atom of azo (N═N) or imino (C═N) groups are of interest due to their π-acceptor properties and redox-active nature, which leads to interesting (opto)electronic properties and reactivity. Here, we describe the synthesis and characterization of rhenium(I) tricarbonyl complexes with neutral N,N-bidentate formazans, which possess both N═N and C═N fragments within the ligand backbone (Ar1-NH-N═C(R3)-N═N-Ar5). The compounds were synthesized by reacting equimolar amounts of [ReBr(CO)5] and the corresponding neutral formazan. X-ray crystallographic and spectroscopic (IR, NMR) characterization confirmed the generation of formazan-type species with the structure fac-[ReBr(CO)3(κ2-N2,N4(Ar1-N1H-N2═C(R3)-N3═N4-Ar5))]. The formazan ligand coordinates the metal center in the 'open' form, generating a five-membered chelate ring with a pendant NH arm. The electronic absorption and emission properties of these complexes are governed by the presence of low-lying π*-orbitals on the ligand as shown by DFT calculations. The high orbital mixing between the metal and ligand results in photophysical properties that contrast to those observed in fac-[ReBr(CO)3(L,L)] species with α-diimine ligands.

Effectiveness of an mHealth intervention to increase adherence to triage of HPV DNA positive women who have performed self-collection (the ATICA study): A hybrid type I cluster randomised effectiveness-implementation trial.

Background: Human Papillomavirus (HPV) self-collection offered by community health workers (CHWs) during home visits has been hampered by low levels of triage Pap among HPV-positive women. We investigated effectiveness of a mHealth intervention to increase adherence to triage Pap. Methods: We conducted a hybrid type I cluster randomised effectiveness-implementation trial in Jujuy, Argentina. CHWs (clusters) were eligible if actively offering HPV self-collection and served at least 26 women aged 30 years and over. Women were eligible if they conducted self-collection and provided a mobile phone number. 260 CHWs were randomly allocated (3:2 ratio) to a multi-component intervention (Up to four SMS messages sent to HPV-positive women, and one SMS message to CHWs to prompt a visit of women with no triage Pap 60 days after a positive-test), or control group (Usual care: Women instructed to attend their health centre 30 days after HPV self-collection to pick-up results). The primary effectiveness outcome was percentage of HPV-positive women with triage 120 days after the HPV-test result. We evaluated implementation of the intervention using the RE-AIM framework. Findings: 221 CHWs (132 intervention, 89 control group) contacted 5389 women; and 5351 agreed to participate (3241 intervention, 2110 control group). In total 314/445 (70·5%) HPV-positive women of the intervention group had triage at 120 days after the HPV result, compared to 163/292 (55·1%) in the control group: 15·5% point improvement; 95%CI: 6·8-24·1; relative risk: 1·28; 95%CI: 1·11-1·48. 97·2% of women accepted the intervention and 86·9% of CHWs agreed to its adoption. Interpretation: The multicomponent mHealth intervention was effective in increasing the percentage of HPV-positive women who had triage Pap, allowing for many more women at risk of cervical cancer to receive timely follow-up. Funding: National Cancer Institute of the National Institutes of Health (USA) under Award Number R01CA218306.

Nursing before COVID-19, a key point for the prevention, control and mitigation of the pandemic.

The SARS-CoV-2 or COVID-19 outbreak originated in China has reached Mexico. However, the scientific community, including nursing, has generated vertiginous evidence that can help make decisions in the care of the affected population. The purpose of this study is to describe the nursing experience before COVID-19 as a key point for the prevention, control and mitigation of the pandemic. Based on the recommendations issued by the Ministry of Health, the clinical evidence and the resources available in the health institutions, the disease prevention measures have been initiated and maintained both in the community and in the health institutions. The reconversion of hospitals and care protocols adapted to our context are trying to strengthen the control and mitigation of the disease.

El brote de SARS-CoV-2 o COVID-19 originado en China ha alcanzado a México; no obstante, la comunidad científica, entre ellas enfermería ha generado de forma vertiginosa evidencia que puede ayudar a tomar decisiones en la atención de la población afectada. El presente documento tiene la finalidad de describir la experiencia de enfermería ante el COVID-19 como un punto clave para la prevención, control y mitigación de la pandemia. Con base en las recomendaciones emitidas por la Secretaría de Salud, la evidencia clínica y los recursos disponibles en las instituciones de salud se ha iniciado y mantenido las medidas de prevención de la enfermedad tanto en la comunidad como en las instituciones de salud. La reconversión de los hospitales y los protocolos de atención adaptados a nuestro contexto están tratando de fortalecer el control y mitigación de la enfermedad.

Towards an anxiety and stress recognition system for academic environments based on physiological features.

BACKGROUND AND OBJECTIVE: Traditional methods to determine stress and anxiety in academic environments consist of the application of questionnaires, but the main disadvantage is that the results depend on the students' self-perception. Being able to detect anxiety-related stress levels in a simple and objective way contributes greatly to dealing with low performance and school drop-out by students. METHODS: The main contribution of this study is to identify the physiological features that could be used as predictors of stressful activities and states of anxiety in academic environments using an Arduino board and low-cost sensors. A test with 21 students was conducted, and a stress-inducing protocol was proposed and 21 physiological features of five signals were analyzed. In addition, the State-Trait Anxiety Inventory (STAI) was used to assess the level of anxiety for each student. Four classifiers were compared to find the physiological feature subset that provides the best accuracy to identify states of stress and anxiety. RESULTS: The stress due to activities performed by students can be identified with an accuracy greater than 90% (Kappa = 0.84) using the k-Nearest Neighbors classifier, using data from heart rate, skin temperature and oximetry signals and four physiological features. Meanwhile, the identification of anxiety was achieved with an accuracy greater than 95% (Kappa = 0.90) using the SVM classifier with data from the galvanic skin response (GSR) signal and three physiological features. CONCLUSIONS: The results provide a clue that anxiety detection in academic environments could be done using the analysis of physiological signals instead of STAI test scores. Besides, the results suggest that physiological features could be used to develop stress recognition systems to help teachers to identify the stressful tasks in an academic environment or to develop anxiety recognition systems to help students to control their level of anxiety when they are performing either academic tasks or exams.

Brownian-particle motion used to characterize mechanical properties of lipid vesicles.

We present a method that takes advantage of the Brownian motion of a colloidal particle to experimentally determine the area stretch modulus of giant liposomes in solution, in regard to the continuum mechanics theory of elasticity. The characteristic parameters of the corresponding model are measured by using the three-dimensional version of digital video microscopy. Such an approach makes use of the diffraction pattern generated by fluorescent spheres found below the focal plane of the microscope objective, allowing the spatial location of the sphere and, thus, the reconstruction of its trajectory. When this particle probe is localized in the neighborhood of a bilayer membrane, its motion causes an elastic distortion of the membrane that is quantifiable. More importantly, such deformation is related to the mentioned modulus, whose measured values are consistent with experimental data obtained through well-known techniques.

Enfermería ante el COVID-19, un punto clave para la prevención, control y mitigación de la pandemia / Nursing before COVID-19, a key point for the prevention, control and mitigation of the pandemic

Resumen El brote de SARS-CoV-2 o COVID-19 originado en China ha alcanzado a México; no obstante, la comunidad científica, entre ellas enfermería ha generado de forma vertiginosa evidencia que puede ayudar a tomar decisiones en la atención de la población afectada. El presente documento tiene la finalidad de describir la experiencia de enfermería ante el COVID-19 como un punto clave para la prevención, control y mitigación de la pandemia. Con base en las recomendaciones emitidas por la Secretaría de Salud, la evidencia clínica y los recursos disponibles en las instituciones de salud se ha iniciado y mantenido las medidas de prevención de la enfermedad tanto en la comunidad como en las instituciones de salud. La reconversión de los hospitales y los protocolos de atención adaptados a nuestro contexto están tratando de fortalecer el control y mitigación de la enfermedad.

Abstract The SARS-CoV-2 or COVID-19 outbreak originated in China has reached Mexico. However, the scientific community, including nursing, has generated vertiginous evidence that can help make decisions in the care of the affected population. The purpose of this study is to describe the nursing experience before COVID-19 as a key point for the prevention, control and mitigation of the pandemic. Based on the recommendations issued by the Ministry of Health, the clinical evidence and the resources available in the health institutions, the disease prevention measures have been initiated and maintained both in the community and in the health institutions. The reconversion of hospitals and care protocols adapted to our context are trying to strengthen the control and mitigation of the disease.

Experimental Determination of High-Order Bending Elastic Constants of Lipid Bilayers.

We present a method to describe the formation of small lipid vesicles in terms of three bending elastic constants that can be experimentally measured. Our method combines a general expression of the elastic free energy of the bilayer and the thermodynamic description of molecular aggregation. The resulting model requires the size distribution of liposomes, which is determined from the X-ray scattered intensity spectra of vesicular dispersions. By using two different preparation methods, we studied a series of vesicular solutions made of distinct lipids and we obtained their corresponding bending elastic constants that are consistent with known bending rigidities.

Endoscopia digestiva en la etapa neonatal: Revisión de la literatura / Digestive endoscopy in the neonatal period: Literature review

Objetivo: Evaluar la eficacia de la endoscopia digestiva diagnóstica y terapéutica en el periodo neonatal. Material y métodos: Se realizó una revisión electrónica en bases de datos MEDLINE y PubMed entre los años 1984-2009 con las siguientes palabras bronchoscopy, endoscopy neonatal. Se incluyeron artículos de revisión bibliográfica y descripción de series de casos. Para el análisis de la información se describen los diferentes artículos. Resultados: Se obtuvieron 850 artículos de endoscopia pediátrica, de los cuales sólo 20 fueron elegidos y revisados para el reporte de resultados de los recién nacidos. Las variables estudiadas fueron indicación, contraindicaciónes, hallazgos y manejo terapéutico. Conclusión: La endoscopia es una herramienta segura y efectiva para el diagnóstico y tratamiento de las diversas entidades patológicas del recién nacido, siempre y cuando se realice por un equipo multidisciplinario.

Objective: To assess the effectiveness of diagnostic and therapeutic bronchoscopy in pediatric age, with emphasis on neonatal period. Material and methods: We performed an electronic review of internet databases MEDLINE and PUBMED, from 1984-2009. The search used the following keywords bronchoscopy, endoscopy and neonatal. The types of study included were: literature review and description of series of cases. For the information analysis, the different articles were described. Results: Of the 850 articles found, only 20 were selected and reviewed. The variables studied were indication, contraindication, findings and therapeutic management. Conclusion: Bronchoscopy is a safe and effective tool for diagnosis and treatment of several pathologies of the new born, when it was carried out by a multidisciplinary team.

Estudio de caso en un paciente postoperado de cirugía cardiovascular con mediastinitis / Study of a case in a patient postoperated of cardiovascular surgery with mediastinitis", "_i": "

Los modelos y teorías de enfermería, así como el proceso deatención de enfermería (PAE) le han proporcionado a estaprofesión una identidad propia y un carácter científico, estableciendocomo único objeto de estudio: el cuidado de la saludde la persona, familia o comunidad. El número creciente de cirugíascardiovasculares que requieren esternotomía se ha vistosólo parcialmente limitado por sus complicaciones. De ellasla mediastinitis post-quirúrgica (MPQ) es una de las másgraves por su alta tasa de morbimortalidad y por que su tratamientodemanda habitualmente de procedimientos quirúrgicosadicionales, además de costosas terapias antimicrobianasy tiempos prolongados de hospitalización, lo que causa un impactosevero en la esfera biopsicosocial de los pacientes y susfamiliares.

Models and Theories of Nursing and the Nursing Care Process(PCN) have provided the identity and profession ascientific character, setting as the sole object of study healthcare of the person, family or community. he increasing numberof cardiovascular surgery requiring sternotomy has beenonly partially limited by its complications. Of these post-surgicalmediastinitis (MPQ) is one of the most serious becauseof its high rate of morbidity and mortality. Its treatmentusually demands additional surgical procedures in additionto costly and prolonged antimicrobial therapy in hospitalwhich causes a severe impact on the biopsychosocial field ofpatients and their families.

Towards cryopreservation of Greenshell mussel (Perna canaliculus) oocytes.

Cryopreservation is a powerful tool for selective breeding in aquaculture as it enables genetic material from selected stock to be stored and crossed at will. The aim of this study was to develop a method for cryopreserving oocytes of the Greenshelltrade mark mussel (Perna canaliculus), New Zealand's main aquaculture species. The ability of oocytes to be fertilized post-thawing was used as the criterion for success in initial experiments and then subsequently, the ability of frozen oocytes to develop further to D-stage larvae was assessed. Ethylene glycol, propylene glycol, dimethyl sulphoxide and glycerol were evaluated at a range of concentrations with and without the addition of 0.2M trehalose using post-thaw fertilization as the endpoint. Ethylene glycol was most effective, particularly when used in combination with trehalose. A more detailed investigation revealed that ethylene glycol at 9% or 10% in the presence of 0.2-0.4M trehalose afforded the best protection. In experiments varying sperm to egg ratio and egg density in post-thaw fertilization procedures, D-larval yield averaged less than 1%. Following these results, a detailed experiment was conducted to determine the damaging steps in the cryopreservation process. Fertilization losses occurred at each step whereas D-larval yield approximately halved following CPA addition and was almost zero following cooling to -10 degrees C. Cryomicroscopy studies and fertilization results suggest that the inability of oocytes to develop to D-larvae stage after cooling to -10 degrees C and beyond are most likely related to some form of chilling injury rather than extracellular ice triggering intracellular ice formation. Further research is needed to determine the causes of this injury and to reduce CPA toxicity and/or osmotic effects.

Cholesterol addition and removal in pacific oyster oocytes does not improve cryopreservation success.

The optimal cholesterol content in cells could provide the benefit of lowering or eliminating the lipid phase transition temperature, while maintaining membrane fluidity and strength; thus, making cells less sensitive to chilling injury and more amenable to cryopreservation. Such effects were shown in some gametes and embryos of certain mammalian species, however, some other cell types, benefited from cholesterol removal. The experiments developed in this study aimed to determine the effect of incubating Pacific oyster (Crassostrea gigas) oocytes in cholesterol-addition or removal solutions prior to cryopreservation on their post-thaw fertilization ability. The results showed a positive association of cholesterol with the oocytes when assessed by fluorescent microscopy. However, this uptake was not reflected by an increase in cholesterol as determined by colorimetric analysis or in the post-thaw fertilization rate of treated oocytes. It is presumed either that oyster oocytes already contain a substantial amount of cholesterol or other lipids in their plasma membranes and do not benefit from any additional cholesterol or there is no lipid phase transition temperature in oyster oocytes.

Determination of the membrane permeability characteristics of Pacific oyster, Crassostrea gigas, oocytes and development of optimized methods to add and remove ethylene glycol.

In order for cryopreservation to become a practical tool for aquaculture, optimized protocols must be developed for each species and cell type. Knowledge of a cell's osmotic tolerance and membrane permeability characteristics can assist in optimized protocol development. In this study, these characteristics were determined for Pacific oyster oocytes and modified methods for loading and unloading ethylene glycol (EG) were tested. Oocytes were found to behave as ideal osmometers and their osmotically inactive fraction (V(b)) was calculated to be 0.48. Oocytes exposed to NaCl solutions of 0.6 to 2.3 Osm fertilized at rates equivalent to oocytes left in seawater. This corresponds to volume changes of +27.3 and -38.1+/-1.2%. The permeability of the oocytes to water (L(p)) was determined to be 3.8+/-0.4 x 10(-2), 5.7+/-0.8 x 10(-2), and 13.2+/-1.3 x 10(-2) microm min(-1)atm(-1), when measured at temperatures of 5, 10 and 20 degrees C. The respective EG permeability values (P(s)) were 9.5+/-0.1 x 10(-5), 14.6+/-1.2 x 10(-5), and 41.7+/-2.4 x 10(-5) cm min(-1). The activation energies for L(p) and P(s) were determined to be 14.5 and 17.5 kcal mol(-1), respectively. Different models for EG loading and unloading from oocytes were developed and tested. Post-thaw fertilization did not differ significantly between a published step addition method and single step addition at 20 degrees C. This represents a considerable reduction in handling. The results of this study demonstrate that the cryobiological characteristics of a given cell type should be taken into account when developing cryopreservation methods.

Survival of Pacific oyster, Crassostrea gigas, oocytes in relation to intracellular ice formation.

The effect of IIF in Pacific oyster oocytes was studied using cryo and transmission electron microscopy (TEM). The viability of oocytes at each step of a published cryopreservation protocol was assessed in an initial experiment. Two major viability losses were identified; one when oocytes were cooled to -35 degrees C and the other when oocytes were plunged in liquid nitrogen. Although the cryomicroscope showed no evidence of IIF in oocytes cooled with this protocol, TEM revealed that these oocytes contained ice crystals and were at two developmental stages when frozen, prophase and metaphase I. To reduce IIF, the effect of seven cooling programmes involving cooling to -35 or -60 degrees C at 0.1 or 0.3 degrees C min(-1) and holding for 0 or 30 min at -35 or -60 degrees C was evaluated on post-thaw fertilization rate of oocytes. Regardless of the cooling rate or holding time, the fertilization rate of oocytes cooled to -60 degrees C was significantly lower than that of oocytes cooled to -35 degrees C. The overall results indicated that observations of IIF obtained from cryomicroscopy are limited to detection of larger amounts of ice within the cells. Although the amount of cellular ice may have been reduced by one of the programmes, fertilization was reduced significantly; suggesting that there is no correlation between the presence of intracellular ice and post-thaw fertilization rate. Therefore, oyster oocytes may be more susceptible to the effect of high solute concentrations and cell shrinkage than intracellular ice under the studied conditions.

Ketoconazol champú al 2 / Ketoconazole 2

Se efectúa el análisis de tratamiento de 30 pacientes que presentaban caspa y dermatitis seborreica. Dieciséis de ellos fueron tratados durante 4 semanas con champú que contiene ketoconazol al 2% y el grupo control (14 pacientes) recibió champú sin ketoconazol. El estudio se efectuó a doble-ciego. Los pacientes que recibieron champú con ketoconazol al 2% demostraron mejoría clínica más rápida, y desaparición total de Pityrosporum ovale en las lesiones, a las 4 semanas de tratamiento. Los pacientes que recibieron placebo no sólo no mejoraron clínicamente sino que en un 64,2% demostraron la permanencia de Pityrosporum ovale en las lesiones. Se destacan las condiciones terapéuticas y cosméticas del champú que contiene ketoconazol al 2%.

Ketoconazol champú al 2 / Ketoconazole 2

Se efectúa el análisis de tratamiento de 30 pacientes que presentaban caspa y dermatitis seborreica. Dieciséis de ellos fueron tratados durante 4 semanas con champú que contiene ketoconazol al 2% y el grupo control (14 pacientes) recibió champú sin ketoconazol. El estudio se efectuó a doble-ciego. Los pacientes que recibieron champú con ketoconazol al 2% demostraron mejoría clínica más rápida, y desaparición total de Pityrosporum ovale en las lesiones, a las 4 semanas de tratamiento. Los pacientes que recibieron placebo no sólo no mejoraron clínicamente sino que en un 64,2% demostraron la permanencia de Pityrosporum ovale en las lesiones. Se destacan las condiciones terapéuticas y cosméticas del champú que contiene ketoconazol al 2%. (AU)