RESUMEN
Maternal nutritional restrictions during late gestation could lead to fetal hypoglycemia. Glucose levels in the fetal sheep regulate circulating insulin-like growth factor 1 (IGF1) levels, which stimulate cell proliferation and differentiation of reproductive organs after binding to its own receptor or estrogen receptors. The objective of this study was to determine the effects of subnutrition of ewes during the last trimester of gestation on the serum glucose/IGF1 levels and development of reproductive organs in their lambs. Pregnant ewes carrying singletons were randomly assigned to restricted (R ewes, nâ¯=â¯8) or control (C ewes, nâ¯=â¯8) groups (4 lambs of each gender/group) and fed with 50% or 100% of metabolic energy requirements from â¼100 days of gestation to term (â¼147 days), respectively. Blood samples from lambs were taken on the first day after born and once at week for serum glucose and IGF1 determination. Lambs were euthanatized at 2 months of age, reproductive organs were weighted and tissue samples were collected from them for histology and to measure mRNA expression of IGF1 and its receptor (IGF1R) by qRT-PCR. Pre-partum glucose levels in R ewes were significantly lower compared to C ewes (pâ¯<â¯.05). Compared to lambs born from C ewes, lambs born from R ewes showed lower serum levels of glucose and IGF1 during the first week of age (pâ¯<â¯.05). At 2 month of age, these lambs had significant lower uterine and testicular weight and lower ovarian, uterine and testicular mRNA expressions of IGF1 and its receptor (pâ¯<â¯.05). Histological findings showed that diameter of secondary and tertiary follicles in ovaries and number of endometrial glands in uterus of females, or number of Sertoli cells and seminiferous tubules and diameter, perimeter and tubular area in testicles of males were significantly lower in lambs born from R ewes compared to the respective organs of lambs born from the C ewes (pâ¯<â¯.05). In conclusion, these results demonstrate that maternal subnutrition during late gestation affects IGF1 levels during fetal life and impairs reproductive development in the neonatal lamb, which could have permanent negative consequences in the future reproductive performance of the offspring.
Asunto(s)
Genitales/crecimiento & desarrollo , Desnutrición/veterinaria , Complicaciones del Embarazo/veterinaria , Fenómenos Fisiologicos de la Nutrición Prenatal , Enfermedades de las Ovejas/fisiopatología , Ovinos/metabolismo , Animales , Glucemia , Estradiol/sangre , Femenino , Factor I del Crecimiento Similar a la Insulina/metabolismo , Masculino , Desnutrición/complicaciones , Embarazo , Ovinos/crecimiento & desarrolloRESUMEN
Proeulia triquetra Obraztsov (Lepidoptera: Tortricidae) is an occasional pest in fruit orchards in central-southern Chile. In order to develop species-specific lures for detection and monitoring of this species, we identified the female-produced sex pheromone. (Z)-11-Tetradecenyl acetate (Z11-14:OAc), (E)-9-dodecenyl acetate (E9-12:OAc), and (E)-11-Tetradecenyl acetate (E11-14:OAc) were identified as biologically active compounds present in female pheromone glands by solvent extraction of the gland and analysis of the extracts by gas chromatography-electroantennographic detection and gas chromatography-mass spectrometry. In field tests, lures baited with synthetic Z11-14:OAc and E9-12:OAc in a 10:1 ratio were highly attractive to males of the species.
Asunto(s)
Ácidos Grasos Monoinsaturados/química , Lepidópteros , Atractivos Sexuales , Animales , Chile , Ácidos Grasos Monoinsaturados/farmacología , Femenino , Masculino , Mariposas NocturnasRESUMEN
OBJECTIVE: To evaluate the association between sociodemographic factors and self-reported use of mouthwashes in a city in the south of Brazil over a time span of 13 years. METHODS: The study involves a comparison of two household surveys conducted in 1996 and 2009. Respectively, 850 and 982 households were included in each year. The mother of the family answered to a structured questionnaire to provide sociodemographic data. Self-reported use of mouthwash was evaluated in a binary manner (yes/no). Multivariate Poisson regression models were used to estimate proportion ratios (PR) and 95% confidence intervals (95% CI). RESULTS: The proportion of mouthwash use increased significantly from 10.8% to 24.2% after 13 years. The probability of using mouthwashes increased more than two times from 1996 to 2009 (PR = 2.25 95% CI 1.80-2.82). Higher probabilities for using mouthwashes were observed in households with higher income (PR = 2.63 95% CI 1.97-3.51) and in those households where the mothers had higher educational levels (PR = 1.46 95% CI 1.05-2.03). CONCLUSION: Higher family income and higher maternal educational level were associated with self-reported use of mouthwashes in a higher proportion of households.
Asunto(s)
Antisépticos Bucales/uso terapéutico , Higiene Bucal/estadística & datos numéricos , Autoinforme , Adulto , Factores de Edad , Brasil , Estudios Transversales , Escolaridad , Composición Familiar , Femenino , Humanos , Renta/estadística & datos numéricos , Persona de Mediana Edad , Madres/educación , Probabilidad , Encuestas y Cuestionarios , Salud Urbana/estadística & datos numéricosRESUMEN
We present five cases of Syringomyelia, three of which are associated with Arnold-Chiari malformation, establishing a comparison between the diagnostic effectivity of CT scan and Nuclear Magnetic Resonance (NMR). In all five cases the lesion was detected by NMR, but not by CT scan. The advantages of NMR over CT scan were evaluated concluding that NMR is more sensible and should be of first choice in the diagnosis of Syringomyelia, permitting also, a better study of the extension of the cavities as well as its control after surgical treatment.