A pro-BMP function exerted by Rhodnius prolixus short gastrulation reveals great diversity in the role of BMP modulators during embryonic patterning.

Dorsal-ventral (DV) patterning is regulated by the bone morphogenetic pathway (BMP) in Bilateria. In insect DV patterning, the Toll pathway also plays a role, in addition to BMPs. Variations in the relative importance of each pathway for DV patterning have been reported using single species of coleopteran, hymenopteran, hemipteran and orthopteran insects. To investigate if the molecular control of DV patterning is conserved inside an insect order, the emergent model hemiptera species Rhodnius prolixus was studied. We found that R. prolixus BMP pathway controls the entire DV axis, with a broader effect respective to Toll, as shown for the hemiptera Oncopeltus fasciatus. Different from O. fasciatus, the unique R. prolixus short gastrulation (sog) and the twisted gastrulation (tsg) orthologues do not antagonize, but rather favour embryonic BMP signalling. Our results reinforce the hypothesis that hemiptera rely preferentially on BMPs for DV patterning but that, surprisingly, in R. prolixus Sog and Tsg proteins exert only a positive role to establish a dorsal-to-ventral BMP gradient. Since sog has been reported to be lost from orthopteran and hymenopteran genomes, our results indicate that Sog's role to modify BMP activity varies greatly in different insect species.

Effect of Simulated Annual At-home Bleaching on Susceptibility to Staining, Translucency, and Whiteness Variations of Computer-aided Design and Computer-aided Manufacturing Monolithic Materials.

The effect of annual at-home bleaching on the color, translucency, and whiteness properties of computer-aided design and computer-aided manufacturing (CAD-CAM) monolithic materials requires investigation. The aim of the present in vitro study was to evaluate the effect of simulated annual at-home bleaching (consisting of daily applications for 10 hours a day for 14 days), for up to 3 years, on susceptibility to staining (ΔE00), translucency (ΔTP00), and whiteness (ΔWID) variations and on topography of CAD-CAM monolithic materials. Disks from the Lava Ultimate (LU), Vita Enamic (VE), IPS Empress CAD (EMP), and IPS e.max CAD (EMAX) were allocated as follows: 1) nonbleached or 2) bleached with 10% carbamide peroxide. After reading the CIE L*a*b* coordinates at baseline (R0), specimens were bleached or not and subsequently immersed for a 1-year simulated period in coffee before the next reading (R1). This process was repeated two more times, resulting in R2 and R3. The ΔE00, ΔTP00, and ΔWID between R1, R2, and R3 in relation to R0 were calculated. The surface topography was analyzed by scanning electron microscopy. In general, bleaching increased the staining susceptibility of all materials when compared to the nonbleached groups and that of the LU, VE, and EMAX over the years. Bleaching decreased the translucency of the VE in all years and over the years. When compared to the nonbleached groups, bleaching decreased the whiteness of the LU and EMAX and increased the whiteness of the EMP, while the VE was not affected. In the LU, the whiteness decreased over the years in both treatments, whereas the other materials were not affected with time. All materials showed progressive topographic changes over the years. The simulated annual at-home bleaching with 10% carbamide peroxide adversely affected the topography and the optical and/or colorimetric properties of the evaluated materials.

Effect of Successive In-office Bleaching Sessions on the Surface Properties, Substance Loss, Biaxial Flexural Strength, and Reliability of CAD-CAM Monolithic Materials.

Little is known about the effects of in-office bleaching on the surface properties and on other aspects of CAD-CAM monolithic materials, especially when successive bleaching sessions are conducted. This study evaluated the effect of up to three successive in-office bleaching sessions on the microhardness, roughness, topography, substance loss, biaxial flexural strength (BFS), and reliability of CAD-CAM monolithic materials. Disks from Lava Ultimate (LU), Vita Enamic (VE), and IPS Empress CAD (EMP) were fabricated and submitted to one, two, and three bleaching sessions. In-office bleaching was performed with 40% hydrogen peroxide and each session consisted of three applications of 20 minutes each. The analyses regarding the aforementioned parameters were assessed before and after the first, second, and third bleaching sessions, except for BFS, which was evaluated at the baseline and after the third session. Weibull modulus was calculated from the BFS data. Bleaching decreased the microhardness of LU and VE from the third and second sessions, respectively, and affected neither the roughness nor the BFS of the three materials. However, in LU, it decreased the reliability and caused topographic changes, which were accentuated by the increase in the number of sessions. In general, LU and EMP showed, respectively, the highest and the lowest substance losses. Successive in-office bleaching sessions with the use of the protocol of the present study should be avoided in LU and VE.

Hyperglycemia alters N-glycans on colon cancer cells through increased production of activated monosaccharides.

Diabetes Mellitus (DM) is both, correlated and a known risk factor for colorectal cancer (CRC). Besides favoring the incidence of CRC, DM also accelerates its progression, worsening its prognosis. Previously, hyperglycemia, the DM hallmark, has been shown to lead to aberrant glycosylation of CRC cells, heightening their malignancy both in vivo and in vitro. Here we use mass spectrometry to elucidate the composition and putative structures of N-glycans expressed by MC38 cultured in normoglycemic (LG) and hyperglycemic-like conditions (HG). N-glycans, 67, were identified in MC38 cells cultured in LG and HG. The cells grown in HG showed a greater abundance of N-glycans when compared to LNG cells, without changes in the proportion of sialylated, fucosylated and mannosylated N-glycans. Among the identified N-glycans, 16 were differentially expressed, mostly mannosylated and fucosylated, with a minority of them being sialylated. Metabolomics analysis indicates that the alterations observed in the N-glycosylation may be mostly due to increase of the activated monosaccharides pool, through an increased glucose entrance into the cells. The alterations found here corroborate data from the literature regarding the progression of CRC, advocating for development or repositioning of effective treatments against CRC in diabetic patients.

Avaliação do ponto de congelamento do leite cru por espectroscopia de infravermelho com transformada de Fourier como método de triagem / [Fourier Transform Infrared spectroscopy (FTIR) as a screening method for freezing point in raw milk]

O objetivo deste estudo foi investigar a interferência do teor de gordura do leite no ponto de congelamento (PC) obtido pelo infravermelho com transformada de Fourier (FTIR). Uma fração de leite cru foi desnatada, obtendo-se creme e leite desnatado. O leite integral e o desnatado foram adicionados com água (0%, 2%, 5%, 10%, 15% e 20%). O leite desnatado remanescente foi adicionado com creme (5%, 10%, 15%, 20%) e água (0%, 2%, 5%, 10%, 15% e 20%). As amostras foram analisadas para PC (crioscópio eletrônico e FTIR) e composição (FTIR). O PC medido por FTIR foi altamente correlacionado com o método do crioscópio eletrônico (acima de 98,5%) e, como esperado, a adição de água foi significativa (P≤0,001) ao aumentar o PC do leite em ambos os métodos. No entanto, o alto teor de gordura no leite cru resultou na redução do PC ao se utilizar o FTIR, enquanto o baixo teor de gordura resultou em aumento do PC (P≤0,001). Uma vez que a adição de água causou o efeito inverso do alto teor de gordura no PC medido por FTIR, é importante considerar o teor de gordura do leite para evitar interpretações erradas do PC quando se utiliza o método FTIR.(AU)

The objective of this study was to investigate the interference of milk fat content upon the Freezing Point (FP) obtained by Fourier-transform infrared (FTIR). A fraction of raw milk was skimmed, obtaining cream and skim milk. Whole and skim milk were added with water (0, 2, 5, 10, 15, and 20%). The remaining skim milk was added with cream (5, 10, 15, 20%) and water (0, 2, 5, 10, 15, and 20%). Samples were analyzed for FP (thermistor cryoscope and FTIR) and composition (FTIR). FP measured by FTIR was highly correlated with the thermistor cryoscope method (above 98.5%) and, as expected, water addition was significant (P≤0.001) upon increasing milk FP in both methods. However, high fat content in raw milk resulted in decreasing FP when using FTIR, while low fat content resulted in increased FP (P≤0.001). Since water addition caused the inverse effect of high fat on FP measured by FTIR, it is important to regard the fat content of milk to avoid misinterpretation of FP when using the FTIR method.(AU)

Análise da estrutura populacional de ovinos deslanados do núcleo de conservação / Population structure analysis in sheep without wool in the conservation nucleus

Este estudo analisou a estrutura populacional de ovinos deslanados do núcleo de conservação do estado do Ceará, Brasil. Os parâmetros populacionais foram estimados com base nos dados genealógicos de indivíduos das raças Santa Inês (SI), Somalis (SO) e Morada Nova (MN), nascidos entre os anos de 2001 e 2014. Os parâmetros estimados foram: número de gerações completas equivalentes (GCE), intervalo entre gerações (IEG), número de fundadores (Nf), número efetivo de fundadores (fe), número efetivo de ancestrais (fa), coeficiente de endogamia (F) e índice de contribuição genética (ICG). O GCE médio foi de 1,82, 2,78 e 1,52 para SI, SO e MN, respectivamente. O IEG foi próximo entre as raças, 3,67 anos em média. O Nf para SI, SO e MN foi igual a 225, 194 e 153, respectivamente. As razões fe/fa foram distantes de 1 nas três populações, o que indica ocorrência de gargalo genético, principalmente para SO. Os coeficientes médios de endogamia foram de 1,81%, 0,78% e 0,78% para SI, SO e MN, respectivamente. O ICG foi de 3,32, 5,38 e 2,87 para SI, SO e MN, respectivamente. Os parâmetros populacionais estimados apontam que parte da genética original desses rebanhos foi perdida, principalmente na população da raça Somalis.(AU)

This study evaluated the population structure of sheep without wool from the conservation nucleus in Ceará State, Brazil. Population parameters were estimated on genealogical records of Santa Ines (SI), Somali (SO,) and Morada Nova (MN) breeds, that were born between 2001 and 2014. The following estimates were obtained: number of complete generation equivalents (GCE), generation intervals (IEG), number of founders (Nf), effective number of founders (fe), effective number of ancestors (fa), inbreeding coefficient (F), and genetic contribution index (ICG). Average GCE was 1.82, 2.78, and 1.52 for SI, SO, and MN respectively. Mean IEG was similar between breeds, 3.67 years. The Nf was 225, 194, and 153 for SI, SO, and MN respectively. The fe/fa ratios were different to 1, which is an indication of genetic bottleneck, mainly for SO. The average inbreeding coefficients were 1.81%, 0.78%, and 0.78% for SI, SO, and MN respectively. The ICG was 3.32, 5.38, and 2.87 for SI, SO, and MN respectively. Estimated population parameters indicate that part of the genetics of these breeds was lost, mainly in Somalis.(AU)

Análise da estrutura populacional de ovinos deslanados do núcleo de conservação / Population structure analysis in sheep without wool in the conservation nucleus

Este estudo analisou a estrutura populacional de ovinos deslanados do núcleo de conservação do estado do Ceará, Brasil. Os parâmetros populacionais foram estimados com base nos dados genealógicos de indivíduos das raças Santa Inês (SI), Somalis (SO) e Morada Nova (MN), nascidos entre os anos de 2001 e 2014. Os parâmetros estimados foram: número de gerações completas equivalentes (GCE), intervalo entre gerações (IEG), número de fundadores (Nf), número efetivo de fundadores (fe), número efetivo de ancestrais (fa), coeficiente de endogamia (F) e índice de contribuição genética (ICG). O GCE médio foi de 1,82, 2,78 e 1,52 para SI, SO e MN, respectivamente. O IEG foi próximo entre as raças, 3,67 anos em média. O Nf para SI, SO e MN foi igual a 225, 194 e 153, respectivamente. As razões fe/fa foram distantes de 1 nas três populações, o que indica ocorrência de gargalo genético, principalmente para SO. Os coeficientes médios de endogamia foram de 1,81%, 0,78% e 0,78% para SI, SO e MN, respectivamente. O ICG foi de 3,32, 5,38 e 2,87 para SI, SO e MN, respectivamente. Os parâmetros populacionais estimados apontam que parte da genética original desses rebanhos foi perdida, principalmente na população da raça Somalis.(AU)

This study evaluated the population structure of sheep without wool from the conservation nucleus in Ceará State, Brazil. Population parameters were estimated on genealogical records of Santa Ines (SI), Somali (SO,) and Morada Nova (MN) breeds, that were born between 2001 and 2014. The following estimates were obtained: number of complete generation equivalents (GCE), generation intervals (IEG), number of founders (Nf), effective number of founders (fe), effective number of ancestors (fa), inbreeding coefficient (F), and genetic contribution index (ICG). Average GCE was 1.82, 2.78, and 1.52 for SI, SO, and MN respectively. Mean IEG was similar between breeds, 3.67 years. The Nf was 225, 194, and 153 for SI, SO, and MN respectively. The fe/fa ratios were different to 1, which is an indication of genetic bottleneck, mainly for SO. The average inbreeding coefficients were 1.81%, 0.78%, and 0.78% for SI, SO, and MN respectively. The ICG was 3.32, 5.38, and 2.87 for SI, SO, and MN respectively. Estimated population parameters indicate that part of the genetics of these breeds was lost, mainly in Somalis.(AU)

Executive functions and memory in bipolar disorders I and II: new insights from meta-analytic results.

OBJECTIVE: To perform a systematic review and meta-analysis of executive functions (EF) and episodic memory in bipolar disorder (BD). METHODS: A literature search was conducted on three electronic databases. Results were combined using random-effects meta-analysis. RESULTS: A total of 126 studies (6424 patients with BDI, 702 with BDII, and 8276 controls) were included. BDI was associated with moderate to large impairments across all cognitive functions and BDII with small-to-medium impairments. Small significant differences were identified between BDI and BDII on all cognitive functions except inhibition. The Trail Making Test (TMT) (g = 0.74, 95% CI: 0.67-0.80), Hayling Test (g = 0.58, 95% CI: 0.34-0.81), Digit Span Total (g = 0.79, 95% CI: 0.57-1.01), and Category Fluency (g = 0.59, 95% CI: 0.45-0.72) tasks were most sensitive to cognitive impairment in BDI. The TMT (g = 0.65, 95% CI: 0.50-0.80) and Category Fluency (g = 0.56, 95% CI: 0.37-0.75) were also sensitive to cognitive alterations in patients with BDII. CONCLUSION: BD type I was associated with more severe and widespread impairments than BDII, which showed smaller impairments on all functions except inhibition, where impairments were larger. Education and (hypo)manic symptoms should be further investigated in future studies due to their possible influence on the neuropsychological profile of BD. The instruments identified in this review should be considered for inclusion in cognitive assessment batteries in BD.

Estimação de parâmetros genéticos para características de pesos e pesos metabólicos na desmama e pós-desmama em bovinos Brahman / Estimation of genetic parameters for weaning weights and metabolic weights characteristics and post weaning in Brahman cattle

Objetivou-se estimar herdabilidades e correlações de características ponderais com 36.505 animais, da Associação Brasileira de Criadores Zebu. O modelo incluiu efeito genético direto, materno, ambiente permanente, residual - aleatórios e efeitos de grupos contemporâneos - fixos. Os parâmetros foram estimados pelo método de máxima verossimilhança restrita (REML), utilizando-se software Wombat. Os resultados das herdabilidades variaram de 0,20 a 0,25 peso à desmama e ao sobreano; 0,16 a 0,20 peso metabólico não ajustado e ajustado à desmama, 0,21 a 0,25 peso metabólico ajustado à desmama e metabólico ajustado ao sobreano. As correlações genéticas entre peso à desmama e peso metabólico não ajustado à desmama, peso à desmama e peso metabólico ajustado à desmama são, respectivamente 0,76 e 1,00. A correlação genética entre peso ao sobreano e metabólico ao sobreano não ajustado, peso ao sobreano com metabólico sobreano ajustado foram 0,97 e 1,00. Correlação genética entre peso à desmama e ao sobreano foi 0,72, peso metabólico não ajustado à desmama e metabólico não ajustado ao sobreano 0,54, peso metabólico ajustado à desmama e metabólico ajustado ao sobreano foi 0,71. Correlações genéticas entre peso à desmama e metabólico ajustado à desmama e peso ano com metabólico ano ajustado foram 1,00 e 1,00. Portanto, utilização de peso metabólico sem ajuste de idade pode viesar estimativas de parâmetros genéticos.(AU)

The objective of this study was to estimate heritability and correlations of 36,505 animals, belonging to the Brazilian Association of Zebu Breeders. They were estimated by Restricted Maximum Likelihood method (REML) using Wombat software. The model included the direct additive genetic effect, maternal, permanent maternal environment and residual as random and fixed effects of contemporary group. The results of heritability ranged from 0,20 to 0,25 for weaning weight and yearling; 0,16 to 0,20 for real metabolic weaning, 0,21 to 0,25 for metabolic adjusted weight at weaning and yearling metabolic adjusted. The genetic correlations between weaning weight with metabolic real, weaning weight adjusted with metabolic are respectively 0,76 and 1,00. The genetic correlation between yearling weight and metabolic real, yearling weight adjusted metabolic were 0,97 and 1,00. Genetic correlations between weaning weight and yearling was 0,72, real metabolic weight at weaning and yearling real metabolic was 0,54, adjusted metabolic weight at weaning and yearling metabolic adjusted was 0,71. Genetic correlations between weight at weaning and adjusted metabolic and weight-adjusted metabolic year were 1,00 and 1,00. Therefore, the use of metabolic weight without age adjustment can warn the estimates.(AU)

Estimação de parâmetros genéticos para características de pesos e pesos metabólicos na desmama e pós-desmama em bovinos Brahman / Estimation of genetic parameters for weaning weights and metabolic weights characteristics and post weaning in Brahman cattle

Objetivou-se estimar herdabilidades e correlações de características ponderais com 36.505 animais, da Associação Brasileira de Criadores Zebu. O modelo incluiu efeito genético direto, materno, ambiente permanente, residual - aleatórios e efeitos de grupos contemporâneos - fixos. Os parâmetros foram estimados pelo método de máxima verossimilhança restrita (REML), utilizando-se software Wombat. Os resultados das herdabilidades variaram de 0,20 a 0,25 peso à desmama e ao sobreano; 0,16 a 0,20 peso metabólico não ajustado e ajustado à desmama, 0,21 a 0,25 peso metabólico ajustado à desmama e metabólico ajustado ao sobreano. As correlações genéticas entre peso à desmama e peso metabólico não ajustado à desmama, peso à desmama e peso metabólico ajustado à desmama são, respectivamente 0,76 e 1,00. A correlação genética entre peso ao sobreano e metabólico ao sobreano não ajustado, peso ao sobreano com metabólico sobreano ajustado foram 0,97 e 1,00. Correlação genética entre peso à desmama e ao sobreano foi 0,72, peso metabólico não ajustado à desmama e metabólico não ajustado ao sobreano 0,54, peso metabólico ajustado à desmama e metabólico ajustado ao sobreano foi 0,71. Correlações genéticas entre peso à desmama e metabólico ajustado à desmama e peso ano com metabólico ano ajustado foram 1,00 e 1,00. Portanto, utilização de peso metabólico sem ajuste de idade pode viesar estimativas de parâmetros genéticos.(AU)

The objective of this study was to estimate heritability and correlations of 36,505 animals, belonging to the Brazilian Association of Zebu Breeders. They were estimated by Restricted Maximum Likelihood method (REML) using Wombat software. The model included the direct additive genetic effect, maternal, permanent maternal environment and residual as random and fixed effects of contemporary group. The results of heritability ranged from 0,20 to 0,25 for weaning weight and yearling; 0,16 to 0,20 for real metabolic weaning, 0,21 to 0,25 for metabolic adjusted weight at weaning and yearling metabolic adjusted. The genetic correlations between weaning weight with metabolic real, weaning weight adjusted with metabolic are respectively 0,76 and 1,00. The genetic correlation between yearling weight and metabolic real, yearling weight adjusted metabolic were 0,97 and 1,00. Genetic correlations between weaning weight and yearling was 0,72, real metabolic weight at weaning and yearling real metabolic was 0,54, adjusted metabolic weight at weaning and yearling metabolic adjusted was 0,71. Genetic correlations between weight at weaning and adjusted metabolic and weight-adjusted metabolic year were 1,00 and 1,00. Therefore, the use of metabolic weight without age adjustment can warn the estimates.(AU)

Identification and Perceived Intensity of Facial Expressions of Emotion in Bipolar Disorder and Major Depression.

OBJECTIVE: This study aimed to examine the accuracy in identifying facial expressions and the perceived intensity of emotions of happiness, sadness, disgust, anger, fear, surprise in bipolar disorder (BD), and major depressive disorder (MDD). METHOD: Ninety-three adult participants (n = 30 BD, 80% female; n = 18 MDD, 72.2% female; n = 45 C, 46.7% female) completed a facial expression task. Stimuli in the task were first presented for 200 ms, followed by 500 ms, and 1000 ms. Participants were asked to identify the emotion expressed by each face and judge its intensity on a Likert scale. Accuracy and perceived intensity of expressions corresponding to each emotion were compared between groups using a repeated measures ANCOVA, with length of stimulus presentation as a within-subjects variable. RESULTS: Expressions of sadness were rated more intensely by patients with BD, and expressions of anger by those with MDD, than control participants. Subjects with BD were less accurate than control participants in identifying expressions of disgust. An interaction effect was also identified for the detection of sadness, whereby patients with BD were significantly less accurate when expressions were shown for 200 ms. CONCLUSIONS: The increased intensity with which emotions were perceived by patients with BD and MDD has important repercussions for patient functioning and clinical practice. A tendency to overestimate the intensity of certain facial expressions in mood disorders may lead patients to interpret social cues erroneously and engage in dysfunctional behaviors and cognitive patterns. Future studies should focus on this variable in addition to the accuracy of emotion identification.

Safety of everolimus plus exemestane in patients with hormone-receptor-positive, HER2-negative locally advanced or metastatic breast cancer: results of phase IIIb BALLET trial in Spain.

BACKGROUND: Everolimus with exemestane has shown promising activity in patients with hormone-receptor (HR)-positive HER2-negative endocrine-resistant advanced breast cancer. It is necessary, therefore, to characterize the safety profile of this new combination in the real-world clinical setting and in the broadest possible population. PATIENTS AND METHODS: Post-menopausal women with HR-positive HER2-negative advanced breast cancer progressing after prior non-steroidal aromatase inhibitors (NSAIs) were included. The objectives of this analysis were to evaluate the safety profile of this combination in a subset of Spanish patients in the BALLET trial and to characterize grade 3 and 4 adverse events (AEs) in routine clinical practice in Spain. RESULTS: Between September 2012 and July 2013, 429 patients (20% of the overall study population) were included in the BALLET study in 52 hospitals in Spain, of whom 100 (23%) were ≥ 70 years. The median treatment duration was 3.14 and 3.03 months for exemestane and everolimus, respectively. The most common reasons for discontinuation of treatment were local reimbursement of everolimus (43%), followed by disease progression (31%) and the incidence of AEs (15%). The most frequent AEs causing permanent discontinuation were pneumonitis (4%), asthenia (2%) and stomatitis (2%). Overall, 87% of patients experienced at least one AE of any grade, 30% of patients at least one grade 3 AE and 2% of patients a grade 4 AE. CONCLUSION: The safety profile in Spanish patients of the BALLET trial is consistent with the results obtained in the overall population of the trial, as well as in previous clinical trials.

A soluble inorganic pyrophosphatase from the cattle tick Rhipicephalus microplus capable of hydrolysing polyphosphates.

Polyphosphates have been found in all cell types examined to date and play diverse roles depending on the cell type. In eukaryotic organisms, polyphosphates have been investigated mainly in mammalian cells, and only a few studies have addressed arthropods. Pyrophosphatases have been shown to regulate polyphosphate metabolism. However, these studies were restricted to trypanosomatids. Here we focus on the tick Rhipicephalus microplus, a haematophagous ectoparasite that is highly harmful to cattle. We produced a recombinant R. microplus pyrophosphatase (rRmPPase) with the aim of investigating its kinetic parameters using polyphosphates as substrate. Molecular docking assays of RmPPase with polyphosphates were also carried out. The kinetic and Hill coefficient parameters indicated that rRmPPase has a greater affinity, higher catalytic efficiency and increased cooperativity for sodium phosphate glass type 15 (polyP15 ) than for sodium tripolyphosphate (polyP3 ). Through molecular docking, we found that polyP3 binds close to the Mg2+ atoms in the catalytic region of the protein, participating in their coordination network, whereas polyP15 interactions involve negatively charged phosphate groups and basic amino acid residues, such as Lys56, Arg58 and Lys193; polyP15 has a more favourable theoretical binding affinity than polyP3 , thus supporting the kinetic data. This study shows, for the first time in arthropods, a pyrophosphatase with polyphosphatase activity, suggesting its participation in polyphosphate metabolism.

Measurements of femoral angles, femur length, and hip width in cat radiographs / Medidas dos ângulos femorais, comprimento do fêmur e largura do quadril em radiografias de gatos

Femoral angle, femur length, and hip width were measured in radiographs of 92 intact domestic cats, males and females of mixed breed from the Center for Zoonosis Control of the Federal District. The animals showed no trauma, orthopedic diseases or angular deformities and had closed physeal lines. Accordingly, we measured aLPFA (anatomical lateral proximal femoral angle, aLDFA (anatomical lateral distal femoral angle), mLPFA (mechanical lateral proximal femoral angle), mLDFA (mechanical lateral distal femoral angle), IA (femoral inclination angle), FL (femur length) and HW (hip width) using ventrodorsal radiographs, with both hindlimbs in a single exposure to an X-ray beam centered on the hip. The mean values of the variables were: mLPFA: 82.5±3.62°; aLPFA: 80.1±4.29°; mLDFA: 96.1±3.51° (males) and 97.3±2.05° (females); aLDFA: 94,3±3.43°; IA: 136.6±3.86°; FL: 12.9±0.55cm (males) and 13.4±0.66cm (females); and HW: 3.1cm±0.23 (males) and 3.5±0.26cm (females). These values will serve as a reference for the diagnosis of angular deformities and as support for planning corrective osteotomies in domestic cats.(AU)

Foram realizadas mensurações radiográficas dos ângulos femorais e medidas lineares do quadril e do fêmur em 92 gatos domésticos, machos e fêmeas, SRD, oriundos do Centro de Controle de Zoonoses do Distrito Federal, livres de traumas, doenças ortopédicas e deformidades angulares em membros pélvicos, portadores de linhas fiseais fechadas, não castrados. Para tanto, foram obtidos os ângulos aLPFA (ângulo anatômico lateral proximal do fêmur), aLDFA (ângulo anatômico lateral distal do fêmur), mLPFA (ângulo mecânico lateral proximal do fêmur), mLDFA (ângulo mecânico lateral distal do fêmur), AI (ângulo de inclinação do fêmur), CF (comprimento femoral) e LQ (largura de quadril), empregando radiografias em projeções ventrodorsais, com ambos os membros em uma única exposição ao feixe de raios-X, centrada no quadril. Após a apuração dos resultados, os valores médios obtidos foram: mLPFA: 82,5°±3,62, aLPFA: 80,1°±4,29; mLDFA: 96,1°±3.51 (machos) e 97,3°±2,05 (fêmeas), aLDFA: 94,3,6°±3,43; AI: 136,6°±3,86; CF: 136,6°±3,86; 12,9cm±0,55 (machos) e 13,4cm±0,66 (fêmeas) e LQ: 3,1cm±0,23 (machos) e 3,5cm±0,26 (fêmeas), respectivamente. Esses valores servem como referenciais para diagnósticos de deformidades angulares e apoio para planejamento de osteotomias corretivas em felinos domésticos.(AU)

Measurements of femoral angles, femur length, and hip width in cat radiographs / Medidas dos ângulos femorais, comprimento do fêmur e largura do quadril em radiografias de gatos

Femoral angle, femur length, and hip width were measured in radiographs of 92 intact domestic cats, males and females of mixed breed from the Center for Zoonosis Control of the Federal District. The animals showed no trauma, orthopedic diseases or angular deformities and had closed physeal lines. Accordingly, we measured aLPFA (anatomical lateral proximal femoral angle, aLDFA (anatomical lateral distal femoral angle), mLPFA (mechanical lateral proximal femoral angle), mLDFA (mechanical lateral distal femoral angle), IA (femoral inclination angle), FL (femur length) and HW (hip width) using ventrodorsal radiographs, with both hindlimbs in a single exposure to an X-ray beam centered on the hip. The mean values of the variables were: mLPFA: 82.5±3.62°; aLPFA: 80.1±4.29°; mLDFA: 96.1±3.51° (males) and 97.3±2.05° (females); aLDFA: 94,3±3.43°; IA: 136.6±3.86°; FL: 12.9±0.55cm (males) and 13.4±0.66cm (females); and HW: 3.1cm±0.23 (males) and 3.5±0.26cm (females). These values will serve as a reference for the diagnosis of angular deformities and as support for planning corrective osteotomies in domestic cats.(AU)

Foram realizadas mensurações radiográficas dos ângulos femorais e medidas lineares do quadril e do fêmur em 92 gatos domésticos, machos e fêmeas, SRD, oriundos do Centro de Controle de Zoonoses do Distrito Federal, livres de traumas, doenças ortopédicas e deformidades angulares em membros pélvicos, portadores de linhas fiseais fechadas, não castrados. Para tanto, foram obtidos os ângulos aLPFA (ângulo anatômico lateral proximal do fêmur), aLDFA (ângulo anatômico lateral distal do fêmur), mLPFA (ângulo mecânico lateral proximal do fêmur), mLDFA (ângulo mecânico lateral distal do fêmur), AI (ângulo de inclinação do fêmur), CF (comprimento femoral) e LQ (largura de quadril), empregando radiografias em projeções ventrodorsais, com ambos os membros em uma única exposição ao feixe de raios-X, centrada no quadril. Após a apuração dos resultados, os valores médios obtidos foram: mLPFA: 82,5°±3,62, aLPFA: 80,1°±4,29; mLDFA: 96,1°±3.51 (machos) e 97,3°±2,05 (fêmeas), aLDFA: 94,3,6°±3,43; AI: 136,6°±3,86; CF: 136,6°±3,86; 12,9cm±0,55 (machos) e 13,4cm±0,66 (fêmeas) e LQ: 3,1cm±0,23 (machos) e 3,5cm±0,26 (fêmeas), respectivamente. Esses valores servem como referenciais para diagnósticos de deformidades angulares e apoio para planejamento de osteotomias corretivas em felinos domésticos.(AU)

Measurements of femoral angles, femur length, and hip width in cat radiographs

ABSTRACT Femoral angle, femur length, and hip width were measured in radiographs of 92 intact domestic cats, males and females of mixed breed from the Center for Zoonosis Control of the Federal District. The animals showed no trauma, orthopedic diseases or angular deformities and had closed physeal lines. Accordingly, we measured aLPFA (anatomical lateral proximal femoral angle, aLDFA (anatomical lateral distal femoral angle), mLPFA (mechanical lateral proximal femoral angle), mLDFA (mechanical lateral distal femoral angle), IA (femoral inclination angle), FL (femur length) and HW (hip width) using ventrodorsal radiographs, with both hindlimbs in a single exposure to an X-ray beam centered on the hip. The mean values of the variables were: mLPFA: 82.5±3.62°; aLPFA: 80.1±4.29°; mLDFA: 96.1±3.51° (males) and 97.3±2.05° (females); aLDFA: 94,3±3.43°; IA: 136.6±3.86°; FL: 12.9±0.55cm (males) and 13.4±0.66cm (females); and HW: 3.1cm±0.23 (males) and 3.5±0.26cm (females). These values will serve as a reference for the diagnosis of angular deformities and as support for planning corrective osteotomies in domestic cats.

RESUMO Foram realizadas mensurações radiográficas dos ângulos femorais e medidas lineares do quadril e do fêmur em 92 gatos domésticos, machos e fêmeas, SRD, oriundos do Centro de Controle de Zoonoses do Distrito Federal, livres de traumas, doenças ortopédicas e deformidades angulares em membros pélvicos, portadores de linhas fiseais fechadas, não castrados. Para tanto, foram obtidos os ângulos aLPFA (ângulo anatômico lateral proximal do fêmur), aLDFA (ângulo anatômico lateral distal do fêmur), mLPFA (ângulo mecânico lateral proximal do fêmur), mLDFA (ângulo mecânico lateral distal do fêmur), AI (ângulo de inclinação do fêmur), CF (comprimento femoral) e LQ (largura de quadril), empregando radiografias em projeções ventrodorsais, com ambos os membros em uma única exposição ao feixe de raios-X, centrada no quadril. Após a apuração dos resultados, os valores médios obtidos foram: mLPFA: 82,5°±3,62, aLPFA: 80,1°±4,29; mLDFA: 96,1°±3.51 (machos) e 97,3°±2,05 (fêmeas), aLDFA: 94,3,6°±3,43; AI: 136,6°±3,86; CF: 136,6°±3,86; 12,9cm±0,55 (machos) e 13,4cm±0,66 (fêmeas) e LQ: 3,1cm±0,23 (machos) e 3,5cm±0,26 (fêmeas), respectivamente. Esses valores servem como referenciais para diagnósticos de deformidades angulares e apoio para planejamento de osteotomias corretivas em felinos domésticos.

Accuracy of genomic selection for age at puberty in a multi-breed population of tropically adapted beef cattle.

Genomic selection is becoming a standard tool in livestock breeding programs, particularly for traits that are hard to measure. Accuracy of genomic selection can be improved by increasing the quantity and quality of data and potentially by improving analytical methods. Adding genotypes and phenotypes from additional breeds or crosses often improves the accuracy of genomic predictions but requires specific methodology. A model was developed to incorporate breed composition estimated from genotypes into genomic selection models. This method was applied to age at puberty data in female beef cattle (as estimated from age at first observation of a corpus luteum) from a mix of Brahman and Tropical Composite beef cattle. In this dataset, the new model incorporating breed composition did not increase the accuracy of genomic selection. However, the breeding values exhibited slightly less bias (as assessed by deviation of regression of phenotype on genomic breeding values from the expected value of 1). Adding additional Brahman animals to the Tropical Composite analysis increased the accuracy of genomic predictions and did not affect the accuracy of the Brahman predictions.

Estimates of genetic parameters for reproductive traits in Brahman cattle breed.

This study was designed to estimate genetic parameters for the following traits of Brahman cattle in Brazil: age at first calving (AFC), calving interval (CI), rebreeding (REB), and stayability (STAY). For REB, the value 1 was assigned to heifers that rebred and calved after first calving and the value 0 was assigned to heifers that failed to rebreed after first calving. Likewise, for STAY, the value 1 was assigned to cows that calved at least 3 times by the time they reach 6 yr of age; otherwise, the value 0 was assigned. A bivariate analysis was used to estimate covariances components by using linear animal model for CI and AFC and threshold animal model for REB and STAY. The mean h(2) were 0.10, 0.02, 0.22, and 0.10 for AFC, CI, REB, and STAY, respectively. The genetic correlations were ­0.13 between AFC and CI, ­0.35 between AFC and REB, ­0.57 between AFC and STAY, and 0.32 between REB and STAY, which reveal that cows that remain productive for longer periods in the herd also start breeding younger and present greater chances to REB. The selection of Brahman cattle for reproductive traits, such as AFC, CI, REB, and STAY, will render low magnitude and long-term responses.