RESUMEN
Quantitative analysis of amino acids in blood and urine is primarily indicated for the diagnosis of amino acid disorders. The high-performance liquid chromatography (HPLC) technique is frequently used for this detection. The frequency of sample collection on filter paper has been increasing exponentially, and there are many advantages attributed to processing biological samples in this way. The aim of this study was to validate a quantitative analysis of amino acids by HPLC in blood and urine collected on filter paper and to establish reference values in the neonatal period. Dried blood and dried urine samples of respectively 58 and 45 healthy newborns (2-9 days) were collected. Pre-treatment and extraction of samples were done according to the literature. Separation and analysis of amino acids were carried out by HPLC with fluorescence detection. The developed method demonstrated excellent separation, linearity, limits of detection and quantification, repeatability and recovery. The reference values for 17 amino acids were defined in dried blood and urine samples of newborns. This work presents a simple, fast and effective method for the simultaneous analysis of 17 amino acids in blood and urine collected on filter paper in a single run. The reference values were established and validated.
Asunto(s)
Aminoácidos , Cromatografía Líquida de Alta Presión/métodos , Pruebas con Sangre Seca/métodos , Tamizaje Neonatal , Aminoácidos/sangre , Aminoácidos/orina , Femenino , Humanos , Recién Nacido , Límite de Detección , Modelos Lineales , Masculino , Errores Innatos del Metabolismo/diagnóstico , Tamizaje Neonatal/métodos , Tamizaje Neonatal/normas , Valores de Referencia , Reproducibilidad de los ResultadosRESUMEN
The activity of Eremanthus erythropappus oil (EO) and some of its compounds and their potential synergistic interaction with ampicillin against different strains of Staphylococcus aureus were investigated. Determination of chemical composition of EO by gas chromatography-mass spectrometry (GC/MS) and bioguided chemical fractionation led to the identification of beta-bisabolene as the main active compound. A synergistic bactericidal activity of EO or beta-bisabolene with ampicillin against Staphylococcus aureus was observed in a time-kill assay. EO and beta- bisabolene have the potential to restore the effectiveness of ampicillin against resistant S. aureus.
