Testing and optimizing metabarcoding of iDNA from dung beetles to sample mammals in the hyperdiverse Neotropics.

Over the past few years, insects have been used as samplers of vertebrate diversity by assessing the ingested-derived DNA (iDNA), and dung beetles have been shown to be a good mammal sampler given their broad feeding preference, wide distribution and easy sampling. Here, we tested and optimized the use of iDNA from dung beetles to assess the mammal community by evaluating if some biological and methodological aspects affect the use of dung beetles as mammal species samplers. We collected 403 dung beetles from 60 pitfall traps. iDNA from each dung beetle was sequenced by metabarcoding using two mini-barcodes (12SrRNA and 16SrRNA). We assessed whether dung beetles with different traits related to feeding, nesting and body size differed in the number of mammal species found in their iDNA. We also tested differences among four killing solutions in preserving the iDNA and compared the effectiveness of each mini barcode to recover mammals. We identified a total of 50 mammal OTUs (operational taxonomic unit), including terrestrial and arboreal species from 10 different orders. We found that at least one mammal-matching sequence was obtained from 70% of the dung beetle specimens. The number of mammal OTUs obtained did not vary with dung beetle traits as well as between the killing solutions. The 16SrRNA mini-barcode recovered a higher number of mammal OTUs than 12SrRNA, although both sets were partly non-overlapping. Thus, the complete mammal diversity may not be achieved by using only one of them. This study refines the methodology for routine assessment of tropical mammal communities via dung beetle 'samplers' and its universal applicability independently of the species traits of local beetle communities.

Pervasive gaps in Amazonian ecological research.

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%-18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost.

Dung beetles from two sustainable-use protected forests in the Brazilian Amazon.

Background: The Amazon Forest is one of the world's most biodiverse ecosystems and yet its protected areas are understudied concerning insects and other invertebrates. These organisms are essential for tropical forests due to their ecological processes, with some species being very sensitive to habitat disturbances. Dung beetles (Coleoptera, Scarabaeidae, Scarabaeinae) have been used as bioindicators for more than 30 years and were surveyed to assess the insect biodiversity of two sustainable-use forest reserves in the Brazilian Amazon. New information: We report inventories of dung beetles from two Amazonian forest reserves in Pará State, Brazil: the Tapajós National Forest and the Carajás National Forest. Surveys were carried out with baited-pitfall traps installed in 2010, 2016, 2017 and 2019. We collected a total of 3,772 individuals from 19 genera and 96 species. We highlight the importance of Amazonian protected areas as refugia for insect biodiversity, particularly dung beetles, which contribute to many key ecosystem processes.

Strong floristic distinctiveness across Neotropical successional forests.

Forests that regrow naturally on abandoned fields are important for restoring biodiversity and ecosystem services, but can they also preserve the distinct regional tree floras? Using the floristic composition of 1215 early successional forests (≤20 years) in 75 human-modified landscapes across the Neotropic realm, we identified 14 distinct floristic groups, with a between-group dissimilarity of 0.97. Floristic groups were associated with location, bioregions, soil pH, temperature seasonality, and water availability. Hence, there is large continental-scale variation in the species composition of early successional forests, which is mainly associated with biogeographic and environmental factors but not with human disturbance indicators. This floristic distinctiveness is partially driven by regionally restricted species belonging to widespread genera. Early secondary forests contribute therefore to restoring and conserving the distinctiveness of bioregions across the Neotropical realm, and forest restoration initiatives should use local species to assure that these distinct floras are maintained.

Linking land-use and land-cover transitions to their ecological impact in the Amazon.

Human activities pose a major threat to tropical forest biodiversity and ecosystem services. Although the impacts of deforestation are well studied, multiple land-use and land-cover transitions (LULCTs) occur in tropical landscapes, and we do not know how LULCTs differ in their rates or impacts on key ecosystem components. Here, we quantified the impacts of 18 LULCTs on three ecosystem components (biodiversity, carbon, and soil), based on 18 variables collected from 310 sites in the Brazilian Amazon. Across all LULCTs, biodiversity was the most affected ecosystem component, followed by carbon stocks, but the magnitude of change differed widely among LULCTs and individual variables. Forest clearance for pasture was the most prevalent and high-impact transition, but we also identified other LULCTs with high impact but lower prevalence (e.g., forest to agriculture). Our study demonstrates the importance of considering multiple ecosystem components and LULCTs to understand the consequences of human activities in tropical landscapes.

Tracking the impacts of El Niño drought and fire in human-modified Amazonian forests.

With humanity facing an unprecedented climate crisis, the conservation of tropical forests has never been so important - their vast terrestrial carbon stocks can be turned into emissions by climatic and human disturbances. However, the duration of these effects is poorly understood, and it is unclear whether impacts are amplified in forests with a history of previous human disturbance. Here, we focus on the Amazonian epicenter of the 2015-16 El Niño, a region that encompasses 1.2% of the Brazilian Amazon. We quantify, at high temporal resolution, the impacts of an extreme El Niño (EN) drought and extensive forest fires on plant mortality and carbon loss in undisturbed and human-modified forests. Mortality remained higher than pre-El Niño levels for 36 mo in EN-drought-affected forests and for 30 mo in EN-fire-affected forests. In EN-fire-affected forests, human disturbance significantly increased plant mortality. Our investigation of the ecological and physiological predictors of tree mortality showed that trees with lower wood density, bark thickness and leaf nitrogen content, as well as those that experienced greater fire intensity, were more vulnerable. Across the region, the 2015-16 El Niño led to the death of an estimated 2.5 ± 0.3 billion stems, resulting in emissions of 495 ± 94 Tg CO2 Three years after the El Niño, plant growth and recruitment had offset only 37% of emissions. Our results show that limiting forest disturbance will not only help maintain carbon stocks, but will also maximize the resistance of Amazonian forests if fires do occur.

Improving the spatial-temporal analysis of Amazonian fires.

There is a growing interest in Amazonian fires, accompanied by a substantial increase in research in the subject. Here, we list five common misunderstandings about Amazonian climate, vegetation, fires and the deforestation process to help to support future research.

Quantifying and addressing the prevalence and bias of study designs in the environmental and social sciences.

Building trust in science and evidence-based decision-making depends heavily on the credibility of studies and their findings. Researchers employ many different study designs that vary in their risk of bias to evaluate the true effect of interventions or impacts. Here, we empirically quantify, on a large scale, the prevalence of different study designs and the magnitude of bias in their estimates. Randomised designs and controlled observational designs with pre-intervention sampling were used by just 23% of intervention studies in biodiversity conservation, and 36% of intervention studies in social science. We demonstrate, through pairwise within-study comparisons across 49 environmental datasets, that these types of designs usually give less biased estimates than simpler observational designs. We propose a model-based approach to combine study estimates that may suffer from different levels of study design bias, discuss the implications for evidence synthesis, and how to facilitate the use of more credible study designs.

Climatic and local stressor interactions threaten tropical forests and coral reefs.

Tropical forests and coral reefs host a disproportionately large share of global biodiversity and provide ecosystem functions and services used by millions of people. Yet, ongoing climate change is leading to an increase in frequency and magnitude of extreme climatic events in the tropics, which, in combination with other local human disturbances, is leading to unprecedented negative ecological consequences for tropical forests and coral reefs. Here, we provide an overview of how and where climate extremes are affecting the most biodiverse ecosystems on Earth and summarize how interactions between global, regional and local stressors are affecting tropical forest and coral reef systems through impacts on biodiversity and ecosystem resilience. We also discuss some key challenges and opportunities to promote mitigation and adaptation to a changing climate at local and global scales. This article is part of the theme issue 'Climate change and ecosystems: threats, opportunities and solutions'.

Clarifying Amazonia's burning crisis.

This article clarifies the different types of fire in the Amazon, their different drivers and the positive feedbacks that can lead to more fires in the region. It then explores evidence regarding the peak in active fire detections in August 2019, showing that these were linked to the highest levels of deforestation since 2008. Finally, we examine the solutions needed to reduce the prevalence of uncontrolled or illegal fire in the Brazilian Amazon.

Efeitos da massagem terapêutica em recém-nascidos prematuros na unidade de terapia intensiva neonatal: uma revisão sistemática / Effects of therapeutic massage on premature newborn in the neonatal intensive therapy unit: a systematic review

INTRODUÇÃO: A Unidade de Terapia Intensiva Neonatal (UTIN) é o local para recém-nascidos de risco que necessitam de cuidados contínuos. A massagem terapêutica é um toque sistemático das mãos humanas que contribui para o ganho de peso, diminui as respostas à dor e diminui a permanência no hospital. OBJETIVO: Revisar os efeitos sistêmicos da massagem terapêutica em prematuros Na Unidade de Terapia Intensiva Neonatal. METODOLOGIA: Esta é uma revisão sistemática. Foi realizada uma busca nas bases de dados Pubmed, Scielo e Lilacs, sem restrições relacionadas ao ano de publicação. Apenas ensaios clínicos randomizados foram incluídos neste estudo, com uma amostra de indivíduos de ambos os sexos, com menos de 28 dias de idade e abordando massoterapia relacionada a respostas sistêmicas em prematuros na unidade de terapia intensiva neonatal. Foram excluídos os estudos relacionados ao exercício físico, uso de medicamentos, estimulação oral e efeitos gastrointestinais relacionados à massagem. Foram utilizados os seguintes descritores: "massagem" AND "recém-nascido prematuro" OR "prematuro" OR "prematuro" AND "unidades de terapia intensiva neonatal". RESULTADOS: Dos seis artigos incluídos nesta revisão, cinco apresentaram resultados satisfatórios sobre os efeitos sistêmicos da massagem terapêutica em recém-nascidos prematuros, como ganho de peso, diminuição da permanência na UTIN, melhora da função do sistema nervoso parassimpático e aumento da citotoxicidade celular de células assassinas naturais. Apenas um estudo relata que a massagem terapêutica não induz o sono. CONCLUSÃO: A massagem terapêutica produz efeitos benéficos sistêmicos em prematuros internados na Unidade de Terapia Intensiva Neonatal.

INTRODUCTION: The Neonatal Intensive Care Unit (NICU) is the place for at-risk newborns who need ongoing care. Massage therapy is a systematic touch by human hands that contributes to weight gain, decreases pain responses and decreases hospital stay. OBJECTIVE: To review the systemic effects of massage therapy on premature infants in the Neonatal Intensive Care Unit. METHODOLOGY: This is a systematic review. A search was performed in the PubMed, SciELO and LILACS database, with no restrictions related to the year of publication. Only randomized clinical trials were included in this study, with a sample of individuals of both sexes, under 28 days of age and addressing massage therapy related to systemic responses in premature infants in the neonatal intensive care unit. Studies that were related to physical exercise, medication use, oral stimulation and gastrointestinal effects related to massage were excluded. The following descriptors were used: "massage" AND "premature newborn" OR "premature" OR "preterm" AND "neonatal intensive care units". RESULTS: Of the six articles included in this literature review, five showed satisfactory results on the systemic effects of massage therapy on premature newborns, such as weight gain, decreased length of stay in the NICU, improvement in parasympathetic nervous system function and increased cell cytotoxicity of natural killer cells. Only one study reports that massage therapy does not induce sleep. CONCLUSION: Massage therapy produces systemic beneficial effects in preterm infants admitted to the Neonatal Intensive Care Unit.

Tree growth and stem carbon accumulation in human-modified Amazonian forests following drought and fire.

Human-modified forests are an ever-increasing feature across the Amazon Basin, but little is known about how stem growth is influenced by extreme climatic events and the resulting wildfires. Here we assess for the first time the impacts of human-driven disturbance in combination with El Niño-mediated droughts and fires on tree growth and carbon accumulation. We found that after 2.5 years of continuous measurements, there was no difference in stem carbon accumulation between undisturbed and human-modified forests. Furthermore, the extreme drought caused by the El Niño did not affect carbon accumulation rates in surviving trees. In recently burned forests, trees grew significantly more than in unburned ones, regardless of their history of previous human disturbance. Wood density was the only significant factor that helped explain the difference in growth between trees in burned and unburned forests, with low wood-density trees growing significantly more in burned sites. Our results suggest stem carbon accumulation is resistant to human disturbance and one-off extreme drought events, and it is stimulated immediately after wildfires. However, these results should be seen with caution-without accounting for carbon losses, recruitment and longer-term changes in species composition, we cannot fully understand the impacts of drought and fire in the carbon balance of human-modified forests.This article is part of a discussion meeting issue 'The impact of the 2015/2016 El Nino on the terrestrial tropical carbon cycle: patterns, mechanisms and implications'.

Quantifying immediate carbon emissions from El Niño-mediated wildfires in humid tropical forests.

Wildfires produce substantial CO2 emissions in the humid tropics during El Niño-mediated extreme droughts, and these emissions are expected to increase in coming decades. Immediate carbon emissions from uncontrolled wildfires in human-modified tropical forests can be considerable owing to high necromass fuel loads. Yet, data on necromass combustion during wildfires are severely lacking. Here, we evaluated necromass carbon stocks before and after the 2015-2016 El Niño in Amazonian forests distributed along a gradient of prior human disturbance. We then used Landsat-derived burn scars to extrapolate regional immediate wildfire CO2 emissions during the 2015-2016 El Niño. Before the El Niño, necromass stocks varied significantly with respect to prior disturbance and were largest in undisturbed primary forests (30.2 ± 2.1 Mg ha-1, mean ± s.e.) and smallest in secondary forests (15.6 ± 3.0 Mg ha-1). However, neither prior disturbance nor our proxy of fire intensity (median char height) explained necromass losses due to wildfires. In our 6.5 million hectare (6.5 Mha) study region, almost 1 Mha of primary (disturbed and undisturbed) and 20 000 ha of secondary forest burned during the 2015-2016 El Niño. Covering less than 0.2% of Brazilian Amazonia, these wildfires resulted in expected immediate CO2 emissions of approximately 30 Tg, three to four times greater than comparable estimates from global fire emissions databases. Uncontrolled understorey wildfires in humid tropical forests during extreme droughts are a large and poorly quantified source of CO2 emissions.This article is part of a discussion meeting issue 'The impact of the 2015/2016 El Niño on the terrestrial tropical carbon cycle: patterns, mechanisms and implications'.

Drought-induced Amazonian wildfires instigate a decadal-scale disruption of forest carbon dynamics.

Drought-induced wildfires have increased in frequency and extent over the tropics. Yet, the long-term (greater than 10 years) responses of Amazonian lowland forests to fire disturbance are poorly known. To understand post-fire forest biomass dynamics, and to assess the time required for fire-affected forests to recover to pre-disturbance levels, we combined 16 single with 182 multiple forest census into a unique large-scale and long-term dataset across the Brazilian Amazonia. We quantified biomass, mortality and wood productivity of burned plots along a chronosequence of up to 31 years post-fire and compared to surrounding unburned plots measured simultaneously. Stem mortality and growth were assessed among functional groups. At the plot level, we found that fire-affected forests have biomass levels 24.8 ± 6.9% below the biomass value of unburned control plots after 31 years. This lower biomass state results from the elevated levels of biomass loss through mortality, which is not sufficiently compensated for by wood productivity (incremental growth + recruitment). At the stem level, we found major changes in mortality and growth rates up to 11 years post-fire. The post-fire stem mortality rates exceeded unburned control plots by 680% (i.e. greater than 40 cm diameter at breast height (DBH); 5-8 years since last fire) and 315% (i.e. greater than 0.7 g cm-3 wood density; 0.75-4 years since last fire). Our findings indicate that wildfires in humid tropical forests can significantly reduce forest biomass for decades by enhancing mortality rates of all trees, including large and high wood density trees, which store the largest amount of biomass in old-growth forests. This assessment of stem dynamics, therefore, demonstrates that wildfires slow down or stall the post-fire recovery of Amazonian forests.This article is part of a discussion meeting issue 'The impact of the 2015/2016 El Niño on the terrestrial tropical carbon cycle: patterns, mechanisms and implications'.

The future of hyperdiverse tropical ecosystems.

The tropics contain the overwhelming majority of Earth's biodiversity: their terrestrial, freshwater and marine ecosystems hold more than three-quarters of all species, including almost all shallow-water corals and over 90% of terrestrial birds. However, tropical ecosystems are also subject to pervasive and interacting stressors, such as deforestation, overfishing and climate change, and they are set within a socio-economic context that includes growing pressure from an increasingly globalized world, larger and more affluent tropical populations, and weak governance and response capacities. Concerted local, national and international actions are urgently required to prevent a collapse of tropical biodiversity.

Does selective logging stress tropical forest invertebrates? Using fat stores to examine sublethal responses in dung beetles.

The increased global demand for tropical timber has driven vast expanses of tropical forests to be selectively logged worldwide. While logging impacts on wildlife are predicted to change species distribution and abundance, the underlying physiological responses are poorly understood. Although there is a growing consensus that selective logging impacts on natural populations start with individual stress-induced sublethal responses, this literature is dominated by investigations conducted with vertebrates from temperate zones. Moreover, the sublethal effects of human-induced forest disturbance on tropical invertebrates have never been examined. To help address this knowledge gap, we examined the body fat content and relative abundance of three dung beetle species (Coleoptera: Scarabaeinae) with minimum abundance of 40 individuals within each examined treatment level. These were sampled across 34 plots in a before-after control-impact design (BACI) in a timber concession area of the Brazilian Amazon. For the first time, we present evidence of logging-induced physiological stress responses in tropical invertebrates. Selective logging increased the individual levels of fat storage and reduced the relative abundance of two dung beetle species. Given this qualitative similarity, we support the measurement of body fat content as reliable biomarker to assess stress-induced sublethal effects on dung beetles. Understanding how environmental modification impacts the wildlife has never been more important. Our novel approach provides new insights into the mechanisms through which forest disturbances impose population-level impacts on tropical invertebrates.

First report on dung beetles in intra-Amazonian savannahs in Roraima, Brazil / Primeiro registro de besouros rola-bosta em savanas intra-amazônicas de Roraima, Brasil

This is the first study to address the dung beetle (Coleoptera: Scarabaeidae: Scarabaeinae) diversity in intra-Amazonian savannahs in the state of Roraima, Brazil. Our aim was to survey the dung beetle fauna associated with these savannahs (regionally called 'lavrado'), since little is known about the dung beetles from this environment. We conducted three field samples using pitfall traps baited with human dung in savannah areas near the city of Boa Vista during the rainy seasons of 1996, 1997, and 2008. We collected 383 individuals from ten species, wherein six have no previous record in intra-Amazonian savannahs. The most abundant species were Ontherus appendiculatus (Mannerheim, 1829), Canthidium aff. humerale (Germar, 1813), Dichotomius nisus (Olivier, 1789), and Pseudocanthon aff. xanthurus (Blanchard, 1846). We believe that knowing the dung beetles diversity associated with the intra-Amazonian savannahs is ideal for understanding the occurrence and distribution of these organisms in a highly threatened environment, it thus being the first step towards conservation strategy development.

Este é o primeiro estudo da diversidade de besouros rola-bosta (Coleoptera: Scarabaeidae: Scarabaeinae) nas savanas intra-amazônicas de Roraima, Brasil. Nosso objetivo foi inventariar a fauna de besouros associada com savanas (conhecidas regionalmente como 'lavrado'), uma vez que pouco se sabe sobre esses besouros nesse ambiente. Nós amostramos em savanas próximas a cidade de Boa Vista durante a estação chuvosa nos anos 1996, 1997 e 2008, utilizando armadilhas pitfall iscadas com fezes humanas. Coletamos 383 indivíduos de dez espécies, das quais seis não haviam ocorrência prévia para savanas intra-amazônicas. As espécies mais abundantes foram Ontherus appendiculatus (Mannerheim, 1829), Canthidium aff. humerale (Germar, 1813), Dichotomius nisus (Olivier, 1789) e Pseudocanthon aff. xanthurus (Blanchard, 1846). Nós acreditamos que conhecendo a diversidade de besouros associada ès savanas intra-Amazônicas se torna ideal para o entendimento da ocorrência e distribuição desses organismos em um ambiente altamente ameaçado, sendo o primeiro passo para a tomada de estratégias de conservação.

Os sentidos do sentido: uma leitura fenomenológica / The meanings of meaning: a phenomenological reading / Los sentidos del sentido: una lectura fenomenologica

Neste artigo, procedemos a uma leitura fenomenológica da noção de sentido e suas múltiplas significações. Partindo de uma primeira visada às definições apresentadas ao termo nos verbetes dos dicionários comuns, tal multiplicidade de significações é discutida à luz do conceito husserliano de "intencionalidade" e compreendida a partir da proposta merleau-pontyana de "reabilitação do sensível". Retomamos, então, o termo sentido desde suas acepções físicas e sensoriais até aquelas de cunho idealizado, relacional e teleológico, considerando-as como um conjunto expresso num único termo e que aponta para uma vida consciente baseada no campo da experiência corporal pré-predicativa desdobrando-se em experiência reflexiva, intersubjetiva e transcendental. Desta forma, o vocábulo sentido mostra-se como uma espécie de multiplicidade unificada e, por isso, considerado como que paradigmático: pura "mostração" do processo perceptivo, diante do qual se tem a contradição-continuidade da imanência (o dado imediatamente) e da transcendência (o que vai além do imediatamente dado). Discutimos as implicações desse entendimento para uma psicologia que se queira eficaz no seu processo de compreender a experiência humana fundamental em sua inserção no mundo da vida...

In this article we carried out a phenomenological reading of the notion of meaning and its multiple meanings. Starting from an initial target to the definitions provided in the dictionaries term, such a multiplicity of meanings is discussed in light of the Husserlian concept of "intentionality" and understood from the Merleau-Ponty propose about "rehabilitation of the sensible." Getting back the term direction from its physical and sensory meanings to those of idealized nature, relational and teleological, considering them as a whole expressed in a single term and points to a conscious life based in the field of body experience prepredicative unfolding in reflective experience, intersubjective and transcendental. Thus, the word order shows up as a kind of multiplicity unified and, therefore, considered that paradigm: pure "showing" the perceptual process, before which one has the contradiction-continuity of immanence (the immediately data) and transcendence (what goes beyond the immediately given). We discuss the implications of this understanding to a psychology that is effective in the process of understanding the fundamental human experience inserted in the living world...

En este texto, llevamos a cabo una lectura fenomenológica del concepto sentido y sus múltiples significados. Partiendo de un primero enfoque sobre las definiciones del término en los artículos de los diccionarios comunes, la multiplicidad de significados es examinada a la luz de la noción intencionalidad en Husserl y comprendida desde la propuesta de "rehabilitación de lo sensible" de Merleau-Ponty. Tomamos entonces el sentido del término desde sus significados sensoriales y físicos a los de naturaleza idealizada, teleológico y relacional, considerándolos como un conjunto que se expresa en un solo término y que apunta a una vida consciente fundada en el terreno de la experiencia pre-predicativa del cuerpo, con desdoblamientos en los terrenos reflexivo, intersubjetivo y trascendental. De esta manera, el sentido de la palabra se muestra como una especie de multiplicidad unificado y por lo tanto, lo consideramos paradigmático: es una demonstración del proceso perceptual, en lo cual tenemos la contradicción-continuidad de la inmanencia (lo inmediatamente dado) y la trascendencia (que va más allá de lo que se da de modo inmediato). Analizaremos las implicaciones de este entendimiento para una psicología que quisiera ser eficaz en su proceso de comprensión de la experiencia humana fundamental insertada en el mundo de la vida...