Integrative multi-omics analyses of date palm (Phoenix dactylifera) roots and leaves reveal how the halophyte land plant copes with sea water.

Date palm (Phoenix dactylifera L.) is able to grow and complete its life cycle while being rooted in highly saline soils. Which of the many well-known salt-tolerance strategies are combined to fine-tune this remarkable resilience is unknown. The precise location, whether in the shoot or the root, where these strategies are employed remains uncertain, leaving us unaware of how the various known salt-tolerance mechanisms are integrated to fine-tune this remarkable resilience. To address this shortcoming, we exposed date palm to a salt stress dose equivalent to seawater for up to 4 weeks and applied integrative multi-omics analyses followed by targeted metabolomics, hormone, and ion analyses. Integration of proteomic into transcriptomic data allowed a view beyond simple correlation, revealing a remarkably high degree of convergence between gene expression and protein abundance. This sheds a clear light on the acclimatization mechanisms employed, which depend on reprogramming of protein biosynthesis. For growth in highly saline habitats, date palm effectively combines various salt-tolerance mechanisms found in both halophytes and glycophytes: "avoidance" by efficient sodium and chloride exclusion at the roots, and "acclimation" by osmotic adjustment, reactive oxygen species scavenging in leaves, and remodeling of the ribosome-associated proteome in salt-exposed root cells. Combined efficiently as in P. dactylifera L., these sets of mechanisms seem to explain the palm's excellent salt stress tolerance.

Shoot chloride translocation as a determinant for NaCl tolerance in Vicia faba L.

Faba bean (Vicia faba L.) is sensitive to salinity. While toxic effects of sodium (Na+) are well studied, toxicity aspects of chloride (Cl-) and the underlying tolerance mechanisms to Cl- are not well understood. For this reason, shoot Cl- translocation and its effect as potential determinant for tolerance was tested. Diverse V. faba varieties were grown hydroponically and stressed with 100 mM NaCl until necrotic leaf spots appeared. At this point, biomass formation, oxidative damage of membranes as well as Na+, Cl- and potassium concentrations were measured. The V. faba varieties contrasted in the length of the period they could withstand the NaCl stress treatment. More tolerant varieties survived longer without evolving necrosis and were less affected by inhibitory effects on photosynthesis. The concentration of Cl- at the time point of developing leaf necrosis was in the same range irrespective of the variety, while that of Na+ varied. This indicates that Cl- concentrations, and not Na+ concentrations are critical for the formation of salt necrosis in faba bean. Tolerant varieties profited from lower Cl- translocation to leaves. Therefore, photosynthesis was less affected in those varieties with lower Cl-. This mechanism is a new trait of interest for salt tolerance in V. faba.