"Pick your team wisely": A case study of a long-standing research-practice partnership.

Research-practice partnerships (RPP) provide opportunities to connect researchers, practitioners, and/or community development professionals in meaningful ways, thereby improving the quality of research, evaluation, and practice in communities. Yet, there is still much to learn about how successful partnerships between researchers and practitioners develop. Historically, research on this topic has emphasized challenges that prevent RPP from developing to their highest potential, with limited information available on ways to promote RPP and how to ensure RPP are mutually beneficial for participants. However, research on partnerships more generally, such as multisector partnerships, can contribute meaningfully to the RPP field. We present an in-depth case study on a successful, long-term partnership. In this study, we pull from the broader partnership literature to explore contexts that are likely to promote partnerships, as well as the activities that can strengthen RPP. Findings focus on addressing ways to promote RPP and highlight the importance of preexisting factors, such as a culture that values both researcher and practitioner expertise, history, and chemistry between partners. Additionally, non-professional space and time, productive disagreements, clear but evolving roles, and shared power were found to strengthen collaboration within the partnership. Specific advice for anyone considering joining an RPP is also discussed.

Acceptability factors for wildlife approach in park and protected area settings.

Human-wildlife conflicts are a challenge in parks and protected areas around the world. Facilitating quality wildlife viewing experiences that minimize negative impacts to people and animals is often complicated by distance-related human behaviors. The purpose of this study was to examine how people's distance-related norms varied as a function of proximity from wildlife, wildlife species, and physical landscape features. By using virtual reality technology and simulations, this study assessed how physical landscape features (i.e., an open field, deadfall, and a paved road) impact wildlife viewing norms and also compared norms among three different wildlife species (i.e., bison, bear, and elk). Data were analyzed with repeated measures ANOVA to explore how these factors influenced acceptability ratings of distances between people and wildlife. Results revealed a significant interaction between distance to wildlife and landscape features. Recommendations for improving the management of humanwildlife conflicts and future research directions are discussed.

Mutualism Wildlife Value Orientations Predict Support for Messages About Distance-Related Wildlife Conflict.

Wildlife value orientations (WVOs) are used to address human-wildlife conflicts by developing value-framed messaging. However, little is known about whether WVOs (i.e., mutualism, domination) are useful in distance-related wildlife safety communication strategies. This study explores the predictive ability of mutualism and domination value orientations on study participants' levels of agreement with distance-related safety messages. Distance-related wildlife safety messages were crafted to appeal to the four WVO typologies (i.e., traditionalist, mutualist, pluralist, and distanced). Undergraduate students were asked to rate their levels of agreement with a series of distance-related messages. Confirmatory factor analyses were used to create domination and mutualism dimension scores. Multiple regressions predicted levels of agreement with each of the messages from domination and mutualism. Study results indicated that in this sample of undergraduate student participants, only mutualism values predicted distance-related wildlife safety message agreement. This information can be used to help frame communications about distance-related wildlife issues.

Arthrogenic neuromusculature inhibition: a foundational investigation of existence in the hip joint.

BACKGROUND: Patients and athletes with diminished gluteal muscle activation are thought to have 'gluteal inhibition'. This may be a component of arthrogenic neuromuscular inhibition, which has been well documented in the knee and generalized to all joints. While clinical evidence surrounding gluteal inhibition increases, supportive research is non-existent. This study investigated whether arthrogenic neuromuscular inhibition occurred about the hip following instillation of intra-articular fluid during functional hip extension tasks. METHODS: Data was collected in a biomechanics laboratory (control) and hospital setting (intervention). Nine healthy individuals (4M/5F) comprised the control group. The intervention group contained twelve patients (4M/8F) with hip pathology requiring a magnetic resonance arthrogram (capsular distension via intra-articular fluid injection) procedure. The participants performed a pelvic bridge (PB) and active hip extension (EXT) before and after the control time or injection. Peak EMG from the gluteus maximus (GM) was collected bilaterally. FINDINGS: The findings of this study provide substantial support for arthrogenic inhibition following hip intra-articular fluid instillation during functional tasks. Two-way repeated measures ANOVA revealed a significant group by session interaction effect (PB,EXT: affected/unaffected=0.0192/0.9654 P=0.05, <0.0001/0.0826 P=0.05). Tukey post hoc revealed decreases in ipsilateral peak GM EMG following intervention were significant (0.0238/<0.0001 P=0.025). No changes were observed in the control group. INTERPRETATION: These concepts are of clinical importance to both patient and athletic populations. Understanding the role of gluteal inhibition in the injury process is essential to the development of rehabilitation and prevention protocols. Restoration and promotion of optimal recruitment patterns are crucial to enhancing athletic performance.

Comparison of recruitment efforts targeted at primary care physicians versus the community at large for participation in Alzheimer disease clinical trials.

Inefficient and delayed recruitment into clinical trials in Alzheimer disease are major obstacles impeding progress in the discovery of more effective therapeutic strategies to combat this disease. Despite widespread recognition of this problem, limited empirical data demonstrating the effectiveness of specific recruitment strategies are available to guide recruitment endeavors. This study was designed to evaluate the effectiveness of recruitment efforts targeting either the primary care health professionals (PCPs) or patients and families with a community grass-roots outreach event. The primary outcome measure was actual study recruitment and participation in the 4 months postintervention. No research subjects were recruited from the PCP intervention, whereas 69 subjects were recruited into clinical studies from the community grass-roots outreach event activity (0% vs. 28%, P<0.0001, Fisher exact test). Barriers to recruitment success in the PCP arm included a perception of perceived harm to subjects from research participation and fear of losing patients through clinical research participation. Our results suggest that outreach efforts directed at the potential study subject/caregiver are not only cost-effective but are able to easily accomplish the desired result of direct recruitment into clinical research studies.

Red blood cell arginase suppresses Jurkat (T cell) proliferation by depleting arginine.

BACKGROUND: Transfusion of packed red blood cells (PRBC) suppresses immunity, but the mechanisms are incompletely understood. PRBCs contain arginase, an enzyme which converts arginine to ornithine and depletes arginine in vitro. Arginine depletion suppresses proliferation of Jurkat T cells in other models. We hypothesize that PRBC arginase-mediated arginine depletion will suppress proliferation of T cells. METHODS: A transfusion model was designed adding PRBC to culture RPMI media with or without an irreversible arginase blocker (nor-NOHA), incubating for 6-48 hours and then removing the PRBCs. Amino acid concentrations in the media were measured using liquid chromatography mass spectrometry. T cells were then added to the pre-conditioned media, cultured for 24 hours, and proliferation was measured. RESULTS: PRBC depleted arginine significantly and increased ornithine in media compared to baseline PRBC treated wells and significantly decreased T cell proliferation. These effects were enhanced with volume of PRBC exposure. Nor-NOHA inhibition of arginase restored T cell proliferation in PRBC treated cultures. CONCLUSIONS: Jurkat T cell proliferation was impaired by PRBC in clinically relevant volumes. The mechanism influencing T cell impairment appears to result from arginine depletion by arginase. Arginine depletion by PRBC arginase may be a novel mechanism for immunosuppression after transfusion.

Molecular characterization of mesophilic and thermophilic sulfate reducing microbial communities in expanded granular sludge bed (EGSB) reactors.

The microbial communities established in mesophilic and thermophilic expanded granular sludge bed reactors operated with sulfate as the electron acceptor were analyzed using 16S rRNA targeted molecular methods, including denaturing gradient gel electrophoresis, cloning, and phylogenetic analysis. Bacterial and archaeal communities were examined over 450 days of operation treating ethanol (thermophilic reactor) or ethanol and later a simulated semiconductor manufacturing wastewater containing citrate, isopropanol, and polyethylene glycol 300 (mesophilic reactor), with and without the addition of copper(II). Analysis, of PCR-amplified 16S rRNA gene fragments using denaturing gradient gel electrophoresis revealed a defined shift in microbial diversity in both reactors following a change in substrate composition (mesophilic reactor) and in temperature of operation from 30 degrees C to 55 degrees C (thermophilic reactor). The addition of copper(II) to the influent of both reactors did not noticeably affect the composition of the bacterial or archaeal communities, which is in agreement with the very low soluble copper concentrations (3-310 microg l(-1)) present in the reactor contents as a consequence of extensive precipitation of copper with biogenic sulfides. Furthermore, clone library analysis confirmed the phylogenetic diversity of sulfate-reducing consortia in mesophilic and thermophilic sulfidogenic reactors operated with simple substrates.

The medical emergency team and rapid response system: finding, treating, and preventing hypoglycemia.

Administrative and quality improvement processes that occurred in response to one patient's series of critical hypoglycemic events ultimately contributed to systematic improvements in patient safety.

Anastrozole therapy in recurrent ovarian adult granulosa cell tumors: a report of 2 cases.

BACKGROUND: Ovarian sex cord stromal tumors are frequently hormonally active, and adult granulosa cell tumors often demonstrate estrogen receptor positivity. Thus, hormonal agents have been evaluated as potential treatments for advanced stage or recurrent adult granulosa cell tumors. CASE: Two cases of patients with recurrent adult granulosa cell tumors are presented. Each patient received multiple treatment modalities including chemotherapy and had previously progressed on leuprolide. Both patients were started on anastrozole with subsequent normalization of inhibin B levels and clinical exams. They have been maintained on treatment for 14 and 18 months, respectively, and have tolerated the drug without difficulty. CONCLUSION: Aromatase inhibitors may be a viable treatment option for women with advanced stage or recurrent ovarian adult granulosa cell tumors.

Quantifying muscle patterns and spine load during various forms of the push-up.

PURPOSE: This study was conducted to quantify the normalized amplitudes of the abdominal wall and back extensor musculature during a variety of push-up styles. We also sought to quantify their impact on spinal loading by calculating spinal compression and torque generation in the L4-5 area. METHODS: Ten university-age participants, nine males and one female, in good to excellent condition, volunteered to participate in this study. All participants were requested to perform a maximum of 12 different push-up exercises, three trials per exercise. Surface electromyographic data (EMG) were collected bilaterally on rectus abdominis, external oblique, internal oblique, latissimus dorsi, and erector spinae muscles, and unilaterally (right side) on pectoralis major, triceps brachii, biceps brachii, and anterior deltoid muscles. Spine kinetics were obtained using an anatomically detailed model of the torso/spine. RESULTS: This study revealed that more dynamic push-ups (i.e., ballistic, with hand movement) required more muscle activation and higher spine load, whereas placing labile balls under the hands only resulted in modest increases in spine load. Right rectus abdominis (RA) activation was significantly higher than left RA activation during the left hand forward push-up and vice versa for the right hand forward push-up (P < 0.001). External oblique (EO) demonstrated the same switch in dominance during staggered hand push-ups (P < 0.01). The one-arm push-up resulted in the highest spine compression. Skilled participants showed greater synchronicity with peak muscle activation (plyometric type of contractions) during ballistic push-ups. CONCLUSION: These data will help guide exercise selection for individuals with differing training objectives and injury history.