RESUMEN
Activity-guided fractionation of the dichloromethane extract from leaves of Piper fulvescens, using an agar overlay bioautographic method, led to the isolation of three antifungal neolignans identified as conocarpan, eupomatenoid 5 and eupomatenoid 6. The minimal inhibitory concentration of these three neolignans against five fungi strains were determined. Conocarpan showed the widest activity, whereas eupomatenoid 6 was the most active against dermatophytes.
Asunto(s)
Antifúngicos/farmacología , Piperaceae , Benzofuranos/química , Benzofuranos/aislamiento & purificación , Benzofuranos/farmacología , Candida albicans/efectos de los fármacos , Cryptococcus neoformans/efectos de los fármacos , Furanos/química , Furanos/aislamiento & purificación , Furanos/farmacología , Medicina Tradicional , Pruebas de Sensibilidad Microbiana , Microsporum/efectos de los fármacos , Paraguay , Fenoles/química , Fenoles/aislamiento & purificación , Fenoles/farmacología , Extractos Vegetales/química , Extractos Vegetales/farmacología , Hojas de la Planta/química , Saccharomyces cerevisiae/efectos de los fármacos , Trichophyton/efectos de los fármacosRESUMEN
The antifungal activity of aqueous, dichloromethane and methanol extracts from 14 Paraguayan plants used in traditional medicine for the treatment of skin diseases was assayed in vitro by the agar disk diffusion method against 11 fungal strains comprising several filamentous fungi and yeasts. Among them, the dichloromethane extracts of Acanthospermum australe, Calycophyllum multiflorum, Geophila repens and Tabebuia avellanedae, as well as the aqueous and methanol extracts of the latter, showed the highest activity.
Asunto(s)
Antifúngicos/farmacología , Hongos/efectos de los fármacos , Medicina Tradicional , Extractos Vegetales/farmacología , Antifúngicos/aislamiento & purificación , Pruebas de Sensibilidad Microbiana , Paraguay , Extractos Vegetales/aislamiento & purificaciónRESUMEN
The dichloromethane extract from the dried flowers of Heterotheca inuloides Cass. was investigated on several pharmacological models of inflammation in vivo and in vitro. It showed anti-inflammatory activity on the croton oil-induced oedema test in mouse ear, at 1 mg/ear. The compound isolated from this extract, 7-hydroxy-3,4-dihydrocadalin, showed anti-inflammatory effect on the same experimental model (ED50 of 0.9 mumol/ear), as well as on COX-1 and COX-2 catalysed prostaglandin biosynthesis assays, with IC50 values of 22 microM and 526 microM, respectively. No effect was observed on carrageenan-induced oedema and on fMLP/PAF-induced exocytosis of human neutrophils. The COX-1 inhibitory effect showed by 7-hydroxy-3,4-dihydrocadalin might be related to the anti-inflammatory activity on the topical oedema induced by croton oil.
