RESUMEN
Adrenocortical disturbances are expected in canine ehrlichiosis due to the immunological challenges caused by infection and consequent inflammation. Thus, this study aimed to evaluate the occurrence of adrenocortical hormonal alterations in dogs naturally infected with Ehrlichia canis (n = 21) as positively confirmed by the presence of anti-E. canis antibodies (Dot-ELISA) and nested PCR (nPCR). Serum dehydroepiandrosterone sulfate (DHEA-S) concentrations were assessed via ELISA before and one hour after ACTH stimulation. Another 10 healthy dogs were subjected to the same stimulation protocol and used as controls. The results revealed that baseline and post-ACTH DHEA-S concentrations were significantly greater in sick dogs, regardless of gender, and this finding illustrates the stress induced by naturally acquired ehrlichiosis in dogs.
Asunto(s)
Sulfato de Deshidroepiandrosterona/sangre , Enfermedades de los Perros/microbiología , Ehrlichia canis/fisiología , Ehrlichiosis/veterinaria , Hormona Adrenocorticotrópica/administración & dosificación , Animales , Enfermedades de los Perros/metabolismo , Perros , Ehrlichiosis/metabolismo , Ehrlichiosis/microbiología , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Masculino , Reacción en Cadena de la Polimerasa/veterinariaRESUMEN
Hemoplasmas are bacteria that infect erythrocytes, attaching to the red blood cell. There is a need for more reports of hemoplasma infection prevalence and molecular characterization among cats in Brazil since there are only few published reports. The present work aimed to detect and molecularly characterize the presence of hemotrophic mycoplasmas in domestic cats with outdoor access from São Luís, Maranhão, Brazil. Twenty cats (10%) were positive for Candidatus M. haemominutum, five (2.5%) for M. haemofelis, and four (2.%) for M. turicensis based on 16S rRNA gene PCRs. Five cats (2.5%) were co-positive for Candidatus M. haemominutum and M. haemofelis. PCR diagnosis was confirmed by sequencing; and phylogenetic analysis was based on 16S rRNA and rnpb genes.
Asunto(s)
Animales , Gatos , Análisis de Secuencia de ADN/métodos , Análisis de Secuencia de ARN/métodos , Gatos , Eritrocitos/patología , Genes de ARNr , Técnicas In Vitro , Infecciones por Mycoplasma , Mycoplasma/genética , Mycoplasma/aislamiento & purificación , Filogenia , Diagnóstico , Métodos , MétodosRESUMEN
Piroplasmosis in donkeys has been recognized as a serious problem of major economic importance, since the affected animals manifest loss of appetite and decreased working capacity. The present work is aimed at detecting infection or exposure of donkeys in São Paulo, Brazil to Theileria (T.) equi and Babesia (B.) caballi using molecular and serological approaches. EDTA-blood and serum samples were collected from 88 donkeys (Equus asinus). From 88 sampled donkeys, 65 (73.86%; 95% confidence interval, PI=63.41, 82.65) and 82 (93.2%; 95% confidence interval, PI=85.75, 97.46) animals showed IgG antibodies to T. equi (by ELISA) and B. caballi (by IFAT), respectively. Twenty-eight (31.81%; 95% confidence interval, PI=22.3, 42.61) and 18 (20.45%; 95% confidence interval, PI=12.6, 30.39) donkeys were positive to T. equi and B. caballi nested PCR assays, respectively. The results indicated that T. equi and B. caballi are prevalent among donkeys in Brazil.
Asunto(s)
Babesia/aislamiento & purificación , Babesiosis/veterinaria , Theileria/aislamiento & purificación , Theileriosis/parasitología , Animales , Babesia/clasificación , Babesiosis/epidemiología , Babesiosis/parasitología , Brasil/epidemiología , Equidae , Estudios Seroepidemiológicos , Pruebas Serológicas , Theileria/clasificación , Theileriosis/epidemiologíaRESUMEN
Hemoplasmas are bacteria that infect erythrocytes, attaching to the red blood cell. There is a need for more reports of hemoplasma infection prevalence and molecular characterization among cats in Brazil since there are only few published reports. The present work aimed to detect and molecularly characterize the presence of hemotrophic mycoplasmas in domestic cats with outdoor access from São Luís, Maranhão, Brazil. Twenty cats (10%) were positive for Candidatus M. haemominutum, five (2.5%) for M. haemofelis, and four (2.%) for M. turicensis based on 16S rRNA gene PCRs. Five cats (2.5%) were co-positive for Candidatus M. haemominutum and M. haemofelis. PCR diagnosis was confirmed by sequencing; and phylogenetic analysis was based on 16S rRNA and rnpb genes.
