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J Agric Food Chem ; 67(2): 688-698, 2019 Jan 16.
Artículo en Inglés | MEDLINE | ID: mdl-30575387

RESUMEN

This study assessed 16 different honey samples in order to select the best one for therapeutic purposes. First, a study of honey's main bioactive compounds was carried out. Then phenolic profiles were determined and specific compounds quantified using a HPLC system coupled to a mass spectrometer. Then, antioxidant activity, by three in vitro methods, and antibacterial activity against reference strains and clinical isolates were evaluated. Great variability among samples was observed regarding ascorbic acid (between 0.34 ± 0.00 and 75.8 ± 0.41 mg/100 g honey; p < 0.001), total phenolic compounds (between 23.1 ± 0.83 and 158 ± 5.37 mg/100 g honey; p < 0.001), and total flavonoid contents (between 1.65 ± 0.11 and 5.93 ± 0.21 mg/100 g honey; p < 0.001). Forty-nine different phenolic compounds were detected, but only 46 of them were quantified by HPLC. The concentration of phenolic compounds and the phenolic profiles varied widely among samples (between 1.06 ± 0.04 and 18.6 ± 0.73 mg/100 g honey; p < 0.001). Antioxidant activity also varied significantly among the samples. All honey varieties exhibited antibacterial activity against both reference and clinical strains (effective concentrations ranged between 0.05 and 0.40 g/mL depending on the honey sample and bacteria tested). Overall, samples with better combinations of bioactive properties were avocado and chestnut honeys.


Asunto(s)
Antibacterianos/química , Antioxidantes/química , Miel/análisis , Antibacterianos/farmacología , Antioxidantes/farmacología , Ácido Ascórbico/análisis , Ácido Ascórbico/farmacología , Bacterias/efectos de los fármacos , Cromatografía Líquida de Alta Presión , Flavonoides/química , Flavonoides/farmacología , Miel/clasificación , Espectrometría de Masas , Fenoles/química , Fenoles/farmacología
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