RESUMEN
OBJECTIVE: Study on the karyotypes analysis of Ferula fukanensis. METHOD: The young roots were treated with 0.000 2 mol x L(1) 8-Hydroxyquinoline for 3 h, carnoy's for 3 h, 1 mol x L(-1) HCl in 5 min,carbol fuchsin coloration for 2 min and the treated roots were utilizied to make the plate for observation. RESULT: The fukanensis is diploid. The chromosome number of somatic cells was 2n = 22. The karyotype formula is 2n = 2x = 20 = 16m + 4sm. The 4th and 10th are submetacentric, and the others are metacentric. CONCLUSION: The karyotype of F. fukanensis belongs to "2A" type of stebbins', and it is a primitive species.
Asunto(s)
Cromosomas de las Plantas/genética , Ferula/genética , Plantas Medicinales/genética , Centrómero , Diploidia , Cariotipificación , Raíces de Plantas/genética , Semillas/genéticaRESUMEN
In this paper we reviewed the development of tissue culture, current situations of virus-free plantlets industrialization and the way to deal with the situations, application prospects of Siraitia grosvenorii so as to give some advice for its further study and application.