Emerging role of exosome-derived non-coding RNAs in tumor-associated angiogenesis of tumor microenvironment.

The tumor microenvironment (TME) is an intricate ecosystem that is actively involved in various stages of cancer occurrence and development. Some characteristics of tumor biological behavior, such as proliferation, migration, invasion, inhibition of apoptosis, immune escape, angiogenesis, and metabolic reprogramming, are affected by TME. Studies have shown that non-coding RNAs, especially long-chain non-coding RNAs and microRNAs in cancer-derived exosomes, facilitate intercellular communication as a mechanism for regulating angiogenesis. They stimulate tumor growth, as well as angiogenesis, metastasis, and reprogramming of the TME. Exploring the relationship between exogenous non-coding RNAs and tumor-associated endothelial cells, as well as their role in angiogenesis, clinicians will gain new insights into treatment as a result.

Analysis of risk factors for recurrence and prognosis of adhesive small bowel obstruction.

OBJECTIVE: The recurrence of adhesive small bowel obstruction (ASBO) limits the effectiveness of clinical treatments, making its significant clinical issues. Clinical features, perioperative parameters, and postoperative outcomes were retrospectively analyzed, to provide basis for screening the high risk factors in patients of recurrent ASBO. METHODS: A review of medical records of patients with ASBO at Chongqing University Central Hospital, from 1 January 2015 to 31 December 2019 was conducted. We compared the clinical characteristics, intra-operative findings, and history surgery of ASBO "relapse-free" and relapsing patients. Logistic proportional hazard model was used to identify recurrence risks. RESULTS: Based on specified inclusion and exclusion criteria, a total of 279 patients were included in this study. Participants' mean age was 63.0 (13.1) years; 49.4% (138 of 279) of them were male. Using multivariate Logistic regression analysis, the history of emergency abdominal surgery (hazard ratio, 0.241, p < 0.0001) was significantly associated with recurrence, as were multiple abdominal surgeries (hazard ratio, 0.250, p < 0.0001) and diabetes mellitus (hazard ratio, 0.182, p < 0.0001). Patients with recurrence, who underwent surgery had longer operative times, blood loss, and a higher incidence of wound complications than those without recurrence. CONCLUSION: The history of emergency abdominal surgery, multiple abdominal surgeries, and diabetes mellitus independently increased the chances of ASBO recurrence. Patients with ASBO recurrence had a higher incidence of postoperative complications.

Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum).

The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity. While gut microbiota plays several roles in fish: well-being, promoting health and growth, resistance to bacterial invasion, regulation of energy absorption, and lipid metabolism. However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits. This study was carried out to evaluate the impacts of supplemented three autochthonous strains, Bacillus sp. RCS1, Pantoea agglomerans RCS2, and Bacillus cereus RCS3 mixture diet on cobia fish (Rachycentron canadum). Also, chromatography, mass spectrometry and high throughput sequencing were combined to explore composition and metabolite profile of gut microbiota in juvenile cobia fed with supplemented diet. In the trial group, juvenile cobia received diets supplemented with 1 × 1012 CFU mL-1 autochthonous strains for ten weeks and a control diet without supplementation. Juvenile cobia receiving diets supplementation exhibited significantly improved growth than those without additives (control). Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group. Similarly, digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities were higher in supplemented diet with an indigenous isolates mixture. Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference. On the other hand, glucose was significantly (P < 0.05) higher in the group without supplementation. On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference. Gut bacterial composition was altered in fish receiving supplemented diet with autochthonous strains. Metabolomics also revealed that some metabolic pathways were considerably enriched in fish fed with supplemented diet; pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed that differentially expressed metabolites were involved in galactose metabolism, tryptophan metabolism, carbohydrate digestion and absorption, purine metabolism, and ABC transporters. Functional analysis of bacterial community showed that differences in enriched metabolic pathways generally comprised carbohydrate and its metabolites, nucleotide and its metabolites, amino acid and its metabolites, heterocyclic compounds, and tryptamines, cholines, pigments. The current investigation results showed that autochthonous strains mixture has significantly enhanced the growth, survival, and innate and adaptive immunities of juvenile cobia.

Properties of mesons in a strong magnetic field.

By extending the [Formula: see text]-derivable approach in the Nambu-Jona-Lasinio model to a finite magnetic field we calculate the properties of pion, [Formula: see text], and [Formula: see text] mesons in a magnetic field at finite temperature not only in the quark-antiquark bound state scheme but also in the pion-pion scattering resonant state scenario. Our calculation as a result makes manifest that the masses of [Formula: see text] and [Formula: see text] meson can be nearly degenerate at the pseudo-critical temperature which increases with increasing magnetic field strength, and the [Formula: see text] mass ascends suddenly at almost the same critical temperature. Meanwhile the [Formula: see text] mesons' masses decrease with the temperature but increase with the magnetic field strength. We also check the Gell-Mann-Oakes-Renner relation and find that the relation can be violated clearly with increasing temperature, and the effect of the magnetic field becomes pronounced around the critical temperature. With different criteria, we analyze the effect of the magnetic field on the chiral phase transition and find that the pseudo-critical temperature of the chiral phase cross, [Formula: see text], is always enhanced by the magnetic field. Moreover, our calculations indicate that the [Formula: see text] mesons will get melted as the chiral symmetry has not yet been restored, but the [Formula: see text] meson does not disassociate even at very high temperature. Particularly, it is the first to show that there does not exist a vector meson condensate in the QCD vacuum in the pion-pion scattering scheme.

Mechanomyography is more sensitive than EMG in detecting age-related sarcopenia.

The aim of this work was to investigate the effects of age-related sarcopenia on the time and frequency domain properties of lower extremity muscles' electromyographic and mechanomyographic activities. Healthy elderly (n=10, 64.5+/-4.5yr) and young (n=10, 22.6+/-2.8yr) were recruited as participants. Participants' lean thigh volumes (LTV) and 1 RM (one repetition maximum) leg strength of quadriceps and maximum speed knee extension with different load levels (45%, 60% and 75% 1 RM) were recorded. The root mean square (RMS) and the mean frequency (MF) of the surface electromyography (EMG(RMS), EMG(MF)) and mechanomyography (MMG(RMS), MMG(MF)) signals were collected at vastus lateralis during concentric contraction with different intensity levels. Compared to the young, the elderly had significantly less LTV, absolute and relative maximal force, as well as absolute and relative maximal power (p<.05). EMG(MF) of the elderly and the young increased monotonically from 45% to 75% 1 RM testing conditions. While the MMG(RMS) of the young increased with testing intensities, the MMG(RMS) of the elderly increased only from 45% to 60% but leveled off from 60% to 75% 1 RM testing conditions. The results indicate the declines of muscle mass, force and power production capacity with aging. The observations could be explained by neuromuscular performance and change of MU activation patterns may result from age-related sarcopenia. Aging affected muscle power more than muscle strength, which could be due to fast fiber reduction. This is supported by our observations that the MMG(RMS) differences between the young and the elderly across all three intensity level where EMG(RMS) was only different at the greatest intensity. We suggest that MMG could be used as an important measurement in studying muscle contraction in age-related sarcopenia.

Distinguishing newly born strange stars from neutron stars with g-mode oscillations.

The gravity-mode (g-mode) eigenfrequencies of newly born strange quark stars (SQSs) and neutron stars (NSs) are studied. It is found that the eigenfrequencies in SQSs are much lower than those in NSs by almost 1 order of magnitude, since the components of a SQS are all extremely relativistic particles while nucleons in a NS are nonrelativistic. We therefore propose that newly born SQSs can be distinguished from the NSs by detecting the eigenfrequencies of the g-mode pulsations of supernovae cores through gravitational radiation by LIGO-class detectors.