Semi-automated assay of erythrocyte Cu-Zn superoxide dismutase activity.

A semi-automated enzymatic colorimetric method for determining Cu-Zn superoxide dismutase (SOD) in erythrocytes using an FP9 parallel analyzer is described. The method is based on the reaction described by McCord and Fridovich (xanthine/xanthine oxidase, ferricytochrome C), which permits kinetic measurements to be determined colorimetrically for the assay of enzyme activity. The coefficients of variation for within- and between-run analyses were less than 6%. The limit of linearity is 3.5-times the mean value of the normal range while recovery of added SOD ranged from 91 to 120%. The method, which is both reliable and simple, allows rapid, simultaneous measurements of multiple samples.

[Free radicals in clinical biology. Origin, pathogenic effect and defense mechanisms]. / Les radicaux libres en biologie clinique: origine, rôle pathogène et moyens de défense.

Free radicals are chemical species with an unpaired electron on their outer orbit. This review discusses the various endogenous and exogenous sources of free radicals; toxicity of free radicals, especially for DNA and cell membranes; a number of pathophysiologic states related to overproduction of free radicals; the natural protective systems--involving enzymes or scavengers--which prevent free radical-induced cell injury, and therapeutic trials of antioxidants for improving protection against free radicals in a number of conditions.