RESUMEN
Drill monkeys (Mandrillus leucophaeus) are an endangered species whose indigenous viral flora is largely unknown. We report here the isolation and characterization of both a cytomegalovirus (DrCMV) and a foamy virus (SFV-drl) from drill monkeys. Phylogenetic analysis of DNA sequence data placed the DrCMV within a primate CMV clade, and showed that SFV-drl was closely related to baboon foamy viruses. ELISA analysis demonstrated that DrCMV shared common epitopes with other primate CMVs but was distinct from human and baboon CMVs. ELISA testing of sera from wild-born and captive-bred drills indicated that the prevalence of antibodies reactive with these two viruses was 93.2% for DrCMV and 20.3% for SFV-drl.
Asunto(s)
Anticuerpos Antivirales/sangre , Citomegalovirus/aislamiento & purificación , Enfermedades de los Monos/virología , Papio/virología , Spumavirus/aislamiento & purificación , Animales , Animales Salvajes , Animales de Zoológico , Citomegalovirus/inmunología , Infecciones por Citomegalovirus/veterinaria , Infecciones por Citomegalovirus/virología , ADN Polimerasa Dirigida por ADN/genética , Datos de Secuencia Molecular , Infecciones por Retroviridae/veterinaria , Infecciones por Retroviridae/virología , Análisis de Secuencia de ADN , Spumavirus/inmunología , Proteínas del Envoltorio Viral/genéticaRESUMEN
The drill monkey has been shown by serology and PCR to harbor a unique simian immunodeficiency virus (SIVdrl). A pol sequence, amplified from uncultured peripheral blood cells, is most closely related to the equivalent SIV sequences from the red-capped mangabey (SIVrcm), the sabaeus African green monkey (SIVagmSAB), and the chimpanzee (SIVcpz) and to the human immunodeficiency virus type 1 (HIV-1) sequence of humans. It is as yet unclear whether SIVdrl has a mosaic genome like SIVrcm and SIVagmSAB, is a member of the SIVcpz/HIV-1 lineage, or represents a novel primate lentivirus lineage.