Sensitivity of populations of bats (Mammalia: Chiroptera) in relation to human development in northern Paraná, southern Brazil.

Most natural forests have been converted for human use, restricting biological life to small forest fragments. Many animals, including some species of bats are disappearing and the list of these species grows every day. It seems that the destruction of the habitat is one of its major causes. This study aimed to analyze how this community of bats was made up in environments with different sizes and quality of habitat. Data from studies conducted in the region of Londrina, Parana, Brazil, from 1982 to 2000 were used. Originally, this area was covered by a semi deciduous forest, especially Aspidosperma polyneuron (Apocynaceae), Ficus insipida (Moraceae), Euterpe edulis (Arecaceae), Croton floribundus (Euforbiaceae), and currently, only small remnants of the original vegetation still exist. The results showed a decline in the number of species caught in smaller areas compared to the largest remnant. In about 18 years of sampling, 42 species of bats were found in the region, representing 67% of the species that occur in Paraná and 24.4% in Brazil. There were two species of Noctilionidae; 21 of Phyllostoma; 11 Vespertilionidae and eight Molossidae. Eight of these were captured only in the largest fragment, Mata dos Godoy State Park (680 ha). Ten species had a low capture rate in the smaller areas with less than three individuals. Of the total sampled, 14 species were found in human buildings, and were able to tolerate modified environments, foraging and even using them as shelter. As the size of the forest area increases, there is a greater variety of ecological opportunities and their physical conditions become more stable, i.e., conditions favorable for growth and survival of a greater number of species. Forest fragmentation limits and creates subpopulations, preserving only long-lived K-strategist animals for some time, where the supporting capacity of the environment is a limiting factor. The reduction of habitats, species and genetic diversity resulting from human activities are endangering the future adaptability in natural ecosystems, which promotes the disappearance of low adaptive potential species.

An AAV2/5 vector enhances safety of gene transfer to the mouse salivary gland.

This study was designed to improve AAV-mediated gene transfer to the murine submandibular salivary glands. Our first aim was to utilize AAV pseudotype vectors, containing the genetic elements of the canonical AAV2, packaged within capsids of AAV serotypes 5, 8, and 9. Having determined that this pseudotyping increased the efficiency of gene transfer to the glands by several orders of magnitude, we next asked whether we could reduce the gene transfer inoculum of the pseudotype while still achieving gene transfer comparable with that achieved with high-dose AAV2. Having achieved gene transfer comparable with that of AAV2 using a pseudotype vector (AAV2/5) at a 100-fold lower dose, our final objective was to evaluate the implications of this lower dose on two pre-clinical parameters of vector safety. To evaluate systemic toxicity, we measured AAV vector sequestration in the liver using qPCR, and found that the 100-fold lower dose reduced the vector recovered from the liver by 300-fold. To evaluate salivary gland function, we undertook whole-proteome profiling of salivary gland lysates two weeks after vector administration and found that high-dose (5 × 109) AAV altered the expression level of ~32% of the entire salivary gland proteome, and that the lower dose (5 × 107) reduced this effect to ~7%.

Origin of a polyploid accession of Brachiaria humidicola (Poaceae: Panicoideae: Paniceae).

Brachiaria humidicola, a species adapted to poorly drained and infertile acid soils, is widely used throughout the tropics. Cytological characterization of 54 accessions of B. humidicola for breeding purposes revealed 2n = 36, 42, and 54 chromosomes. One accession (H030), with 2n = 42 chromosomes, showed a different meiotic behavior. In most accessions from the genus Brachiaria previously studied, the basic chromosome number is x = 9, but the putative basic number in H030 appears to be x = 6. Since six univalent chromosomes were found in diakinesis and metaphase I, and these behaved as laggards in anaphase I, it was hypothesized that both genitors were derived from x = 6, and that this accession is a heptaploid 2n = 7x = 42. The parental genomes did not have the same meiotic behavior, particularly during anaphase, when one genome consisting of six univalents remained as laggards and underwent sister-chromatid segregation. At telophase, 18 segregated chromosomes were found at each pole. The laggard genome did not reach the poles at telophase I or II in time to be included in the nucleus and was eliminated as micronuclei.