RESUMEN
We herein present a case of right triquetral fracture with associated pisiform and flexor carpi ulnaris subluxation in a 29-year-old man. Initial radiography showed a right triquetral fracture. Computed tomography and magnetic resonance imaging demonstrated a triquetral fracture with a subluxated pisiform. Open reduction and lag screw fixation of the right triquetrum was performed, with good subsequent recovery of function. Although triquetral fracture with subluxation of the pisotriquetral joint is uncommon, attending clinicians should bear in mind the possibility of such an injury when managing traumatic ulnar-sided wrist pain.
Asunto(s)
Fracturas Óseas/complicaciones , Accidentes por Caídas , Adulto , Fijación de Fractura/métodos , Fracturas Óseas/diagnóstico por imagen , Humanos , Imagen por Resonancia Magnética , Masculino , Músculo Esquelético/diagnóstico por imagen , Músculo Esquelético/fisiopatología , Tendones/patología , Tomografía Computarizada por Rayos X , Resultado del Tratamiento , Articulación de la Muñeca/patologíaRESUMEN
The 30K proteins are involved with important functions in the growth and development of Bombyx mori. In this study, the synthesis and regulation of 30K proteins were examined during the degeneration of Bombyx silk glands. On day 3 of the fifth instar, the protein level of 30Kc19 was low, whereas the silk proteins were rapidly synthesized. However, synthesis and accumulation of the 30Kc19 protein significantly increased at the prepupal stage and on day 1 of the pupal stage. At this stage, the silk gland cells were filled with 30Kc19 and genomic DNA. Moreover, the transcript levels of the 30K-encoding genes, including 30Kc6, 30Kc12, 30Kc19 and 30Kc23 were up-regulated during the degeneration of the Bombyx silk glands. During the time that the levels of the 30Kc19 protein were significantly up-regulated, it is notable that the transcript levels of the BmAtg8, BmAtg6 and BmDronc genes dramatically increased to regulate the programmed cell death of this gland. On day 1 of the pupal stage, intense fragmentation of genomic DNA occurred in the silk gland cells, and the putative active form of caspase was detected in the cytoplasm, showing the complete degradation of the silk glands in one day. In conclusion, the 30K proteins are synthesized in high concentrations, while proteolysis mediates silk gland degeneration in Bombyx by a caspase-dependent pathway. We propose that the 30K proteins may be nutrients and energy vectors to be absorbed by the developing tissues of pupae or moths.
Asunto(s)
Bombyx/crecimiento & desarrollo , Bombyx/genética , Proteínas de Insectos/genética , Animales , Bombyx/metabolismo , Caspasas/genética , Caspasas/metabolismo , Muerte Celular , Glándulas Exocrinas/crecimiento & desarrollo , Glándulas Exocrinas/metabolismo , Regulación del Desarrollo de la Expresión Génica , Proteínas de Insectos/metabolismo , Larva/genética , Larva/crecimiento & desarrollo , Larva/metabolismo , Especificidad de Órganos , Pupa/genética , Pupa/crecimiento & desarrollo , Pupa/metabolismo , Análisis de Secuencia de ProteínaRESUMEN
The characteristic features of airway hyperreactivity disease are non-specific hyperresponsiveness, insensitive to spasmolysant and damage of epithelium. In our experiment, we measured the tension change of isolated trachea smooth muscle of guinea pig in reacting to acetylcholine and verapamil. It confirmed that (1) the removal of epithelium increases the reactivity of preparations to acetylcholine and reduces the effect of verapamil, (2) in above process the epithelium-derived relaxing factor (EpDRF) had participated in airway hyperreactivity and (3) EpDRF was not derived from metabolism of cyclooxygenase.