RESUMEN
Peste-des-Petits-Ruminants (PPR) or goat plague is an important viral disease of sheep and goats caused by the small ruminant morbilli virus or PPR virus (PPRV). Long non coding RNAs (lncRNA) and circular RNAs (circRNA) play a pivotal role in several biological processes including regulation of virus-host interactions. The present study explored the expression of lncRNA, circRNA and their functions in PPRV infected B-lymphocyte (B95a) cells. The results revealed a total of 4531 lncRNA and 2348 circRNA expression in both mock and PPRV infected samples. Analysis of differentially expressed (DE) RNA identified 123 DE-lncRNA and 39 DE-circRNA as significantly dysregulated. Functional analysis of cis-target genes of DE-lncRNA indicated activation of TCF dependent WNT signaling and PKN1 stimulated transcription process. Interactions (sponging) of microRNA (miRNA) revealed 344 DE-lncRNA-miRNA and 93 DE-circRNA-miRNA pairs. The competing endogenous RNA (ceRNA) network of lncRNA/circRNA-miRNA-mRNA in PPRV infected B95a cells was represented by 69 ceRNA pairs. We validated the DE-circRNA by targeted amplification and sequencing of back spliced junctions (BSJs). The present study revealed a profile of lncRNA, circRNA and their potential ceRNA network in PPRV infection. The results provide insight for better understanding of PPRV-host interactions.
Asunto(s)
Enfermedades de las Cabras , MicroARNs , Peste de los Pequeños Rumiantes , Virus de la Peste de los Pequeños Rumiantes , ARN Largo no Codificante , Enfermedades de las Ovejas , Animales , Linfocitos B , Callithrix/genética , Cabras , MicroARNs/genética , Peste de los Pequeños Rumiantes/genética , Virus de la Peste de los Pequeños Rumiantes/genética , ARN Circular/genética , ARN Largo no Codificante/genética , OvinosRESUMEN
Escherichia coli causes diarrhea and extraintestinal infections in humans and animals. Here, we report the draft genome sequences of Escherichia coli strains 360/16 and 646, isolated from neonatal calves.
RESUMEN
AIMS AND OBJECTIVES: We aimed to compare the hemodynamic effects of levosimendan and dobutamine in patients undergoing mitral valve surgery on cardiopulmonary bypass (CPB). MATERIALS AND METHODS: Sixty patients were divided into 2 groups of 30 each. Group-L patients received levosimendan 0.1 µg/kg/min and Group-D patients received dobutamine 5 µg/kg/min while weaning off CPB. Additional inotrope and/or vasoconstrictor were started based on hemodynamic parameters. Hemodynamic data were collected at the end and at 30 minutes after CPB, thereafter at 6, 12, 24, and 36 hours post-CPB. Mean arterial pressure (MAP), central venous pressure (CVP), heart rate (HR), cardiac index (CI), systemic vascular resistance index (SVRI), and lactate levels were measured. RESULTS: Group-L showed increased requirement of inotropes and vasoconstrictors. The SVRI, CVP, and MAP were reduced more in Group-L. The CI was low in Group-L in the initial period when compared to Group-D. Later Group-L patients showed a statistically significant increase in CI even after 12 hrs of discontinuation of levosimendan infusion. The HR was increased more in Group-D. Lactate levels, intensive care unit stay, and duration of ventilation were similar in both groups. CONCLUSIONS: Levosimendan 0.1 µg/kg/min compared to dobutamine 5 µg/kg/min showed more vasodilation and lesser inotropic activity in patients undergoing mitral valve surgery for mitral stenosis. Levosimendan compared to dobutamine showed a statistically significant increase in CI even after 12 hrs of discontinuation. The requirement of another inotrope or vasopressor was frequent in levosimendan group.
Asunto(s)
Cardiotónicos/farmacología , Dobutamina/farmacología , Implantación de Prótesis de Válvulas Cardíacas/métodos , Hemodinámica/efectos de los fármacos , Hidrazonas/farmacología , Estenosis de la Válvula Mitral/cirugía , Válvula Mitral/cirugía , Piridazinas/farmacología , Adulto , Presión Arterial/efectos de los fármacos , Gasto Cardíaco/efectos de los fármacos , Puente Cardiopulmonar , Presión Venosa Central/efectos de los fármacos , Método Doble Ciego , Femenino , Paro Cardíaco Inducido , Frecuencia Cardíaca/efectos de los fármacos , Humanos , Hipotermia Inducida , Ácido Láctico/sangre , Masculino , Estudios Prospectivos , Simendán , Resultado del Tratamiento , Resistencia Vascular/efectos de los fármacosRESUMEN
This study was designed to study the efficacy of intravenous dexmedetomidine for attenuation of cardiovascular responses to laryngoscopy and endotracheal intubation in patients with coronary artery disease. Sixty adult patients scheduled for elective off-pump coronary artery bypass surgery were randomly allocated to receive dexmedetomidine (0.5 mcg/kg) or normal saline 15 min before intubation. Patients were compared for hemodynamic changes (heart rate, arterial blood pressure and pulmonary artery pressure) at baseline, 5 min after drug infusion, before intubation and 1, 3 and 5 min after intubation. The dexmedetomidine group had a better control of hemodynamics during laryngoscopy and endotracheal intubation. Dexmedetomidine at a dose of 0.5 mcg/kg as 10-min infusion was administered prior to induction of general anaesthesia attenuates the sympathetic response to laryngoscopy and intubation in patients undergoing myocardial revascularization. The authors suggest its administration even in patients receiving beta blockers.
