Development of cytochrome b primers for mitotyping of barbels (Barbus spp.).

Primers were developed that allow for rapid, reliable and inexpensive screening of cytochrome b by analysis of single-stranded conformational polymorphisms. Twenty different haplotypes were identified from six species of Iberian Barbus. These primers proved useful for population and species level studies, and could also be valuable in population genetic and phylogenetic studies of other cyprinin fishes.